WO2004042085A3 - Methode de quantification d'un rapport entre au moins deux sequences d'acides nucleiques - Google Patents

Methode de quantification d'un rapport entre au moins deux sequences d'acides nucleiques Download PDF

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Publication number
WO2004042085A3
WO2004042085A3 PCT/NL2003/000780 NL0300780W WO2004042085A3 WO 2004042085 A3 WO2004042085 A3 WO 2004042085A3 NL 0300780 W NL0300780 W NL 0300780W WO 2004042085 A3 WO2004042085 A3 WO 2004042085A3
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WO
WIPO (PCT)
Prior art keywords
interest
nucleic acids
determining
nucleic acid
ratio
Prior art date
Application number
PCT/NL2003/000780
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English (en)
Other versions
WO2004042085A2 (fr
Inventor
Eveline Catherina A Timmermans
Gemen Bob Van
Original Assignee
Primagen Holding Bv
Eveline Catherina A Timmermans
Gemen Bob Van
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP02079673A external-priority patent/EP1418241A1/fr
Application filed by Primagen Holding Bv, Eveline Catherina A Timmermans, Gemen Bob Van filed Critical Primagen Holding Bv
Priority to JP2004549721A priority Critical patent/JP2006505268A/ja
Priority to AU2003279621A priority patent/AU2003279621A1/en
Priority to NZ540144A priority patent/NZ540144A/en
Publication of WO2004042085A2 publication Critical patent/WO2004042085A2/fr
Publication of WO2004042085A3 publication Critical patent/WO2004042085A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Oncology (AREA)
  • AIDS & HIV (AREA)
  • Microbiology (AREA)
  • Communicable Diseases (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

L'invention concerne une méthode de quantification d'un rapport initial de quantité d'au moins deux acides nucléiques d'intérêt dans un échantillon au moyen d'une réaction d'amplification des acides nucléiques multiplex, consistant:- à amplifier lesdits acides nucléiques d'intérêt dans ladite réaction d'amplification; - à mesurer la quantité d'au moins deux acides nucléiques d'intérêt dans au moins deux repères temporels différents de ladite réaction; - à déterminer à partir d'au moins deux desdites mesures le taux d'amplification desdits acides nucléiques d'intérêt; - à comparer lesdits taux à une référence; et - à déterminer à partir de ladite comparaison ledit rapport initial de quantité desdits acides nucléiques d'intérêt dans ledit échantillon. Au moins un facteur variable de ladite réaction d'amplification d'acides nucléiques est, de préférence, ajusté afin d'atteindre des niveaux détectables de tous les acides nucléiques d'intérêt avant d'atteindre une limite d'amplification et/ou de détection d'un ou de plusieurs acides nucléiques d'intérêt. La méthode de l'invention permet de déterminer de petites différences entre les rapports. Une mesure directe du rapport initial des acides nucléiques peut, de plus, être déterminée. La méthode de l'invention est, par exemple, adaptée pour déterminer le fonctionnement d'un organisme cellulaire, pour déterminer la stadification d'une maladie, et pour déterminer l'activité thérapeutique et/ou les éventuels effets secondaires d'un médicament ou d'un composé candidat.
PCT/NL2003/000780 2002-11-08 2003-11-07 Methode de quantification d'un rapport entre au moins deux sequences d'acides nucleiques WO2004042085A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2004549721A JP2006505268A (ja) 2002-11-08 2003-11-07 少なくとも２つの核酸配列間の比率を定量化する方法
AU2003279621A AU2003279621A1 (en) 2002-11-08 2003-11-07 Method for quantifying a ratio between at least two nucleic acid sequences
NZ540144A NZ540144A (en) 2002-11-08 2003-11-07 Method for quantifying a ratio between at least two nucleic acid sequences

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US42505502P 2002-11-08 2002-11-08
EP02079673.6 2002-11-08
EP02079673A EP1418241A1 (fr) 2002-11-08 2002-11-08 Procédé de quantifier le rapport de deux séquences d'acides nucléiques
US60/425,055 2002-11-08

Publications (2)

Publication Number Publication Date
WO2004042085A2 WO2004042085A2 (fr) 2004-05-21
WO2004042085A3 true WO2004042085A3 (fr) 2004-08-05

Family

ID=32313842

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2003/000780 WO2004042085A2 (fr) 2002-11-08 2003-11-07 Methode de quantification d'un rapport entre au moins deux sequences d'acides nucleiques

Country Status (6)

Country Link
JP (1) JP2006505268A (fr)
KR (1) KR20050088998A (fr)
AU (1) AU2003279621A1 (fr)
CA (1) CA2448769A1 (fr)
NZ (1) NZ540144A (fr)
WO (1) WO2004042085A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA013970B1 (ru) * 2005-03-24 2010-08-30 Тромбоджиникс Н.В. Антитела к плацентарному фактору роста и способы их применения
KR101414714B1 (ko) * 2007-11-20 2014-07-03 삼성전자주식회사 경쟁적 증폭 반응을 이용하여 표적 핵산을 검출하는 방법

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002046470A2 (fr) * 2000-12-04 2002-06-13 Primagen Holding B.V. Analyse d'organites cellulaires endosymbiontes et composes pouvant etre identifies par cette analyse

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002046470A2 (fr) * 2000-12-04 2002-06-13 Primagen Holding B.V. Analyse d'organites cellulaires endosymbiontes et composes pouvant etre identifies par cette analyse

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BAAR DE M P ET AL: "ONE-TUBE REAL-TIME ISOTHERMAL AMPLIFICATION ASSAY TO IDENTIFY AND DISTINGUISH HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 SUBTYPES A,B, AND C AND CIRCULATING RECOMBINANT FORMS AE AND AG", JOURNAL OF CLINICAL MICROBIOLOGY, WASHINGTON, DC, US, vol. 39, no. 5, May 2001 (2001-05-01), pages 1895 - 1902, XP001011572, ISSN: 0095-1137 *
CRAWFORD ERIN L ET AL: "Multiplex standardized RT-PCR for expression analysis of many genes in small samples.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. UNITED STATES 26 APR 2002, vol. 293, no. 1, 26 April 2002 (2002-04-26), pages 509 - 516, XP002249374, ISSN: 0006-291X *
MEIJERINK J ET AL: "A novel method to compensate for different amplification efficiencies between patient DNA samples in quantitative real-time PCR.", THE JOURNAL OF MOLECULAR DIAGNOSTICS: JMD. UNITED STATES MAY 2001, vol. 3, no. 2, May 2001 (2001-05-01), pages 55 - 61, XP002249372, ISSN: 1525-1578 *
MOUTOU CÉLINE ET AL: "Multiplex PCR combining deltaF508 mutation and intragenic microsatellites of the CFTR gene for pre-implantation genetic diagnosis (PGD) of cystic fibrosis.", EUROPEAN JOURNAL OF HUMAN GENETICS: EJHG. ENGLAND APR 2002, vol. 10, no. 4, April 2002 (2002-04-01), pages 231 - 238, XP002249373, ISSN: 1018-4813 *
VAN DEURSEN P B ET AL: "A novel quantitative multiplex NASBA method: application to measuring tissue factor and CD14 mRNA levels in human monocytes.", NUCLEIC ACIDS RESEARCH. ENGLAND 1 SEP 1999, vol. 27, no. 17, 1 September 1999 (1999-09-01), pages e15, XP002249371, ISSN: 1362-4962 *

Also Published As

Publication number Publication date
KR20050088998A (ko) 2005-09-07
WO2004042085A2 (fr) 2004-05-21
AU2003279621A1 (en) 2004-06-07
JP2006505268A (ja) 2006-02-16
NZ540144A (en) 2008-08-29
CA2448769A1 (fr) 2004-05-08

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