WO2004038367A3 - Tests biologiques utilisant des gradients formes dans des systemes microfluidiques - Google Patents

Tests biologiques utilisant des gradients formes dans des systemes microfluidiques Download PDF

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Publication number
WO2004038367A3
WO2004038367A3 PCT/US2003/033146 US0333146W WO2004038367A3 WO 2004038367 A3 WO2004038367 A3 WO 2004038367A3 US 0333146 W US0333146 W US 0333146W WO 2004038367 A3 WO2004038367 A3 WO 2004038367A3
Authority
WO
WIPO (PCT)
Prior art keywords
biological assays
microfluidic systems
present
gradients formed
gradients
Prior art date
Application number
PCT/US2003/033146
Other languages
English (en)
Other versions
WO2004038367A2 (fr
Inventor
Gregory L Kirk
Enoch Kim
Emanuele Ostuni
Olivier Schueller
Paul Sweetnam
Original Assignee
Surface Logix Inc
Gregory L Kirk
Enoch Kim
Emanuele Ostuni
Olivier Schueller
Paul Sweetnam
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Surface Logix Inc, Gregory L Kirk, Enoch Kim, Emanuele Ostuni, Olivier Schueller, Paul Sweetnam filed Critical Surface Logix Inc
Priority to JP2004546909A priority Critical patent/JP2006507815A/ja
Priority to EP03781348A priority patent/EP1578194A4/fr
Priority to AU2003287170A priority patent/AU2003287170A1/en
Priority to CA002503186A priority patent/CA2503186A1/fr
Publication of WO2004038367A2 publication Critical patent/WO2004038367A2/fr
Publication of WO2004038367A3 publication Critical patent/WO2004038367A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nanotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • Materials Engineering (AREA)
  • Microbiology (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Composite Materials (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un dispositif de suivi de chimiotaxie ou de chimio-invasion. Elle concerne aussi un système de test flexible et de nombreux tests pouvant être utilisés afin de tester des systèmes et des interactions biologiques. On utilise à cette fin des gradients d'écoulement laminaire qui miment les situations de gradient présentes in vivo.
PCT/US2003/033146 2002-10-22 2003-10-21 Tests biologiques utilisant des gradients formes dans des systemes microfluidiques WO2004038367A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2004546909A JP2006507815A (ja) 2002-10-22 2003-10-21 ミクロ流体システム内に形成された勾配を使用した生物学的アッセイ
EP03781348A EP1578194A4 (fr) 2002-10-22 2003-10-21 Tests biologiques utilisant des gradients formes dans des systemes microfluidiques
AU2003287170A AU2003287170A1 (en) 2002-10-22 2003-10-21 Biological assays using gradients formed in microfluidic systems
CA002503186A CA2503186A1 (fr) 2002-10-22 2003-10-21 Tests biologiques utilisant des gradients formes dans des systemes microfluidiques

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US41997602P 2002-10-22 2002-10-22
US41998002P 2002-10-22 2002-10-22
US60/419,980 2002-10-22
US60/419,976 2002-10-22

Publications (2)

Publication Number Publication Date
WO2004038367A2 WO2004038367A2 (fr) 2004-05-06
WO2004038367A3 true WO2004038367A3 (fr) 2004-11-11

Family

ID=32179774

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2003/033146 WO2004038367A2 (fr) 2002-10-22 2003-10-21 Tests biologiques utilisant des gradients formes dans des systemes microfluidiques
PCT/US2003/033177 WO2004038368A2 (fr) 2002-10-22 2003-10-21 Dispositif et procede pour surveiller la migration des leucocytes

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2003/033177 WO2004038368A2 (fr) 2002-10-22 2003-10-21 Dispositif et procede pour surveiller la migration des leucocytes

Country Status (5)

Country Link
EP (2) EP1558076A4 (fr)
JP (2) JP2006507815A (fr)
AU (2) AU2003287170A1 (fr)
CA (2) CA2503203A1 (fr)
WO (2) WO2004038367A2 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1773978T3 (da) * 2004-05-19 2014-05-26 Univ Pittsburgh Perfunderede, tredimensionelle celle/vævssygdomsmodeller
US7351423B2 (en) 2004-09-01 2008-04-01 Depuy Spine, Inc. Musculo-skeletal implant having a bioactive gradient
KR100873383B1 (ko) 2007-11-13 2008-12-10 이화여자대학교 산학협력단 선충의 주화성 연구용 미세 유체 소자
KR20110003526A (ko) 2008-04-08 2011-01-12 메사추세츠 인스티튜트 오브 테크놀로지 3차원 미세유체 플랫폼 및 이의 사용 방법
DE102009035502A1 (de) 2009-07-30 2011-02-03 Universitätsklinikum Jena Verfahren und Vorrichtung zur Erfassung der Bewegung und Anlagerung von Zellen und Partikeln an Zell-, Gewebe- und Implantatschichten bei der Simulation von Flussbedingungen
DE102009038520B4 (de) * 2009-08-25 2012-07-05 Universität Rostock Verfahren und Vorrichtung zur Zellprobendiagnostik
CN102580798B (zh) * 2012-02-28 2014-03-05 东北大学 一种微流控芯片溶液梯度稀释装置
CA2908622A1 (fr) * 2012-04-18 2013-10-24 Hemoshear, Llc Modele in vitro pour conditions pathologiques ou physiologiques
JP5910282B2 (ja) * 2012-04-20 2016-04-27 大日本印刷株式会社 細胞遊走解析方法および細胞培養物作成方法
KR101433091B1 (ko) * 2012-04-26 2014-08-25 한국과학기술원 박테리아의 주화성 분석용 마이크로 플루이딕 장치, 제조방법 및 이를 이용한 박테리아의 주화성 분석 방법
WO2015061372A1 (fr) 2013-10-21 2015-04-30 Hemoshear, Llc Modèle in vitro pour micro-environnement tumoral
WO2016065125A1 (fr) * 2014-10-22 2016-04-28 The General Hospital Corporation Procédé et appareil de visualisation d'objets à l'aide d'un rétroéclairage oblique
CN105080627A (zh) * 2015-08-25 2015-11-25 辽宁中医药大学 一种用于药物筛选的集成化微流控芯片及其应用方法
CN106497788A (zh) * 2016-11-28 2017-03-15 南方医科大学珠江医院 体外肝细胞异质性培养装置及其培养方法
JP6931943B2 (ja) * 2017-08-30 2021-09-08 イムプリメド インコーポレイティッドＩｍｐｒｉｍｅｄ，Ｉｎｃ． 化学的及び生化学的化合物の高処理量選別のための製品
CN111019828B (zh) * 2019-12-26 2021-12-21 武汉大学 一种开放式高通量微流控卵母细胞动态三维培养芯片及其应用
IL314493A (en) * 2022-01-28 2024-09-01 Glaxosmithkline Ip Dev Ltd A high-throughput assay for cell migration, chemotaxis and function
CN114887034B (zh) * 2022-07-14 2022-10-28 中山莱博瑞辰生物医药有限公司 LLP2A-Ale在制备用于治疗和/或预防外周血淋巴细胞减少症的药物中的用途

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998052691A1 (fr) * 1997-05-16 1998-11-26 Alberta Research Council Systeme microfluidique et ses utilisations
WO2000073799A1 (fr) * 1999-06-01 2000-12-07 Caliper Technologies Corp. Dosages a micro-echelle et dispositifs microfluidiques destines au controle des activites de transporteur, de gradient induit et de liaison
US20020168757A1 (en) * 2000-11-08 2002-11-14 Gregory Kirk Test device and method of making same

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5284753A (en) * 1991-03-20 1994-02-08 Neuro Probe, Inc. Multiple-site chemotactic test apparatus and method
US5460945A (en) * 1991-05-30 1995-10-24 Center For Blood Research, Inc. Device and method for analysis of blood components and identifying inhibitors and promoters of the inflammatory response
US6632619B1 (en) * 1997-05-16 2003-10-14 The Governors Of The University Of Alberta Microfluidic system and methods of use
US7326563B2 (en) * 2000-11-08 2008-02-05 Surface Logix, Inc. Device and method for monitoring leukocyte migration
WO2003078565A1 (fr) * 2002-03-12 2003-09-25 Surface Logix, Inc. Dispositif pour test de motilite et de chimiotaxie, et son procede d'utilisation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998052691A1 (fr) * 1997-05-16 1998-11-26 Alberta Research Council Systeme microfluidique et ses utilisations
WO2000073799A1 (fr) * 1999-06-01 2000-12-07 Caliper Technologies Corp. Dosages a micro-echelle et dispositifs microfluidiques destines au controle des activites de transporteur, de gradient induit et de liaison
US20020168757A1 (en) * 2000-11-08 2002-11-14 Gregory Kirk Test device and method of making same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JEON ET AL: "Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed ina microfabricated device", NATURE BIOTECH, vol. 20, 1 July 2002 (2002-07-01), pages 826 - 830, XP002978726 *
See also references of EP1578194A4 *

Also Published As

Publication number Publication date
CA2503203A1 (fr) 2004-05-06
WO2004038368A3 (fr) 2004-07-29
AU2003287170A1 (en) 2004-05-13
EP1578194A2 (fr) 2005-09-28
WO2004038367A2 (fr) 2004-05-06
EP1558076A4 (fr) 2007-03-21
WO2004038368A2 (fr) 2004-05-06
JP2006503581A (ja) 2006-02-02
JP2006507815A (ja) 2006-03-09
EP1578194A4 (fr) 2007-03-21
AU2003282950A1 (en) 2004-05-13
CA2503186A1 (fr) 2004-05-06
EP1558076A2 (fr) 2005-08-03

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