WO2004035790A1 - Utilisation d'un promoteur d'inhibiteur bifonctionnel d'alpha-amylase subtilisine pour diriger l'expression dans le tissu maternel de graines de plantes - Google Patents

Utilisation d'un promoteur d'inhibiteur bifonctionnel d'alpha-amylase subtilisine pour diriger l'expression dans le tissu maternel de graines de plantes Download PDF

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Publication number
WO2004035790A1
WO2004035790A1 PCT/AU2003/001381 AU0301381W WO2004035790A1 WO 2004035790 A1 WO2004035790 A1 WO 2004035790A1 AU 0301381 W AU0301381 W AU 0301381W WO 2004035790 A1 WO2004035790 A1 WO 2004035790A1
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WIPO (PCT)
Prior art keywords
plant
nucleic acid
asi
seed
protein
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PCT/AU2003/001381
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English (en)
Inventor
Agnelo Furtado
Robert James Henry
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Molecular Plant Breeding Nominees Ltd
Grains Research And Development Corporation
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Publication date
Application filed by Molecular Plant Breeding Nominees Ltd, Grains Research And Development Corporation filed Critical Molecular Plant Breeding Nominees Ltd
Priority to AU2003271420A priority Critical patent/AU2003271420B2/en
Publication of WO2004035790A1 publication Critical patent/WO2004035790A1/fr

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

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  • Health & Medical Sciences (AREA)
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  • Pharmacology & Pharmacy (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La présente invention concerne un procédé d'expression d'acide nucléique ou de protéine dans le tissu maternel d'une graine de plante, de manière fonctionnelle, sous le contrôle d'une séquence de régulation dérivée d'un gène d'inhibiteur bifonctionnel d'alpha-amylase subtilisine (par exemple, SEQ ID Nos: 1 or 2). De manière plus particulière, cette invention se rapporte à des méthodes de production d'une graine améliorée ou d'un produit du son amélioré du fait de l'expression d'une quelconque protéine hétérologue dans le tissu maternel sous le contrôle du promoteur ASI, tel que, par exemple, une graine ayant une protection renforcée contre un pathogène de plante transmis par la semence, notamment Fusarium graminearum (la gale) et le virus de la striure de l'orge; ou une graine ou un produit du son qui est utile en tant que vaccin oral du fait de l'expression d'une protéine antigénique du virus responsable des gastro-entérites transmissibles ou encore une graine ou un produit du son ayant une valeur nutritive accrue tel que du fourrage du fait de l'expression d'une protéine riche en soufre (protéine de noix du Brésil).
PCT/AU2003/001381 2002-10-17 2003-10-17 Utilisation d'un promoteur d'inhibiteur bifonctionnel d'alpha-amylase subtilisine pour diriger l'expression dans le tissu maternel de graines de plantes WO2004035790A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003271420A AU2003271420B2 (en) 2002-10-17 2003-10-17 Use of bifunctional alpha-amylase subtilisin inhibitor promoter to direct expression in the maternal tissue of a plant seed

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2002952143 2002-10-17
AU2002952143A AU2002952143A0 (en) 2002-10-17 2002-10-17 Use of bifunctional alpha-amylase/subtilisin inhibitor promoter to direct expression in the pericarp of plants

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WO2004035790A1 true WO2004035790A1 (fr) 2004-04-29

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AU (1) AU2002952143A0 (fr)
WO (1) WO2004035790A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006118816A3 (fr) * 2005-04-29 2007-04-12 Pioneer Hi Bred Int Element regulateur prefere du pericarpe
US20180153124A1 (en) * 2016-12-01 2018-06-07 Board Of Trustees Of Michigan State University Fusarium head blight resistance in plants
CN112980847A (zh) * 2021-04-21 2021-06-18 中国热带农业科学院橡胶研究所 一种橡胶树泛素基因启动子proHbUBI3及其克隆与应用
CN113025616A (zh) * 2021-04-21 2021-06-25 中国热带农业科学院橡胶研究所 一种橡胶树泛素基因启动子proHbUBI2及其克隆与应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000018926A1 (fr) * 1998-09-25 2000-04-06 The University Of Queensland UTILISATION D'UNE SEQUENCE PROMOTEUR INHIBITEUR BIFONCTIONNELLE D'α-AMYLASE SUBTILISINE DE L'ORGE POUR CONFERER UNE EXPRESSION DANS DES SEMENCES

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000018926A1 (fr) * 1998-09-25 2000-04-06 The University Of Queensland UTILISATION D'UNE SEQUENCE PROMOTEUR INHIBITEUR BIFONCTIONNELLE D'α-AMYLASE SUBTILISINE DE L'ORGE POUR CONFERER UNE EXPRESSION DANS DES SEMENCES

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PEKKARINEN A. I. ET AL.: "Purification and indentification of barley (Hordeum vulgare L.) proteins that inhibit the alkaline serine proteinases of fusarium culmordum", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 51, no. 6, 2003, pages 1710 - 1717 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006118816A3 (fr) * 2005-04-29 2007-04-12 Pioneer Hi Bred Int Element regulateur prefere du pericarpe
US7550579B2 (en) 2005-04-29 2009-06-23 Pioneer Hi-Bred International, Inc. Pericarp-preferred regulatory element
US7851614B2 (en) 2005-04-29 2010-12-14 Pioneer Hi-Bred International, Inc. Terminator from Zea mays lipid transfer protein 1 gene
US7897746B2 (en) 2005-04-29 2011-03-01 Pioneer Hi-Bred International, Inc. Pericarp-preferred promoter from maize lipid transfer protein gene
US20180153124A1 (en) * 2016-12-01 2018-06-07 Board Of Trustees Of Michigan State University Fusarium head blight resistance in plants
CN112980847A (zh) * 2021-04-21 2021-06-18 中国热带农业科学院橡胶研究所 一种橡胶树泛素基因启动子proHbUBI3及其克隆与应用
CN113025616A (zh) * 2021-04-21 2021-06-25 中国热带农业科学院橡胶研究所 一种橡胶树泛素基因启动子proHbUBI2及其克隆与应用
CN112980847B (zh) * 2021-04-21 2023-05-02 中国热带农业科学院橡胶研究所 一种橡胶树泛素基因启动子proHbUBI3及其克隆与应用
CN113025616B (zh) * 2021-04-21 2023-05-02 中国热带农业科学院橡胶研究所 一种橡胶树泛素基因启动子proHbUBI2及其克隆与应用

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