WO2003104420A3 - Detection electrochimique de polymorphismes touchant un nucleotide unique (snp) - Google Patents

Detection electrochimique de polymorphismes touchant un nucleotide unique (snp) Download PDF

Info

Publication number
WO2003104420A3
WO2003104420A3 PCT/US2003/018093 US0318093W WO03104420A3 WO 2003104420 A3 WO2003104420 A3 WO 2003104420A3 US 0318093 W US0318093 W US 0318093W WO 03104420 A3 WO03104420 A3 WO 03104420A3
Authority
WO
WIPO (PCT)
Prior art keywords
extension reaction
nucleic acid
single nucleotide
primer
snps
Prior art date
Application number
PCT/US2003/018093
Other languages
English (en)
Other versions
WO2003104420A2 (fr
Inventor
Werner G Kuhr
Sara A Brazill
Original Assignee
Univ California
Werner G Kuhr
Sara A Brazill
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Werner G Kuhr, Sara A Brazill filed Critical Univ California
Priority to AU2003251424A priority Critical patent/AU2003251424A1/en
Priority to EP03757435A priority patent/EP1543153A2/fr
Publication of WO2003104420A2 publication Critical patent/WO2003104420A2/fr
Publication of WO2003104420A3 publication Critical patent/WO2003104420A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne de nouveaux procédés de détection de polymorphismes touchant un nucléotide unique (SNP). Dans une forme de réalisation, le procédé consiste à i) utiliser une amorce d'acide nucléique complémentaire à une séquence d'acide nucléique adjacente à l'emplacement du nucléotide unique comprenant ledit polymorphisme touchant un nucléotide unique, ladite amorce étant d'une longueur suffisante pour initier la transcription par une polymérase d'acide nucléique; ii) effectuer une réaction d'allongement d'amorce au moyen d'une polymérase d'acide nucléique, un terminateur de chaîne pour produire au moins un produit de réaction d'allongement, le ou les constituants de ladite réaction d'allongement de l'amorce étant marqués avec un marqueur à activité redox, de sorte qu'au moins un produit de réaction d'allongement produit par la réaction d'allongement d'amorce soit marqué avec ledit marqueur à activité redox, la détection d'un produit de réaction d'allongement plus long que l'amorce d'acide nucléique indiquant la présence dudit polymorphisme touchant un nucléotide unique dans ledit acide nucléique cible.
PCT/US2003/018093 2002-06-07 2003-06-06 Detection electrochimique de polymorphismes touchant un nucleotide unique (snp) WO2003104420A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003251424A AU2003251424A1 (en) 2002-06-07 2003-06-06 Electrochemical detection of single nucleotide polymorphisms (snps)
EP03757435A EP1543153A2 (fr) 2002-06-07 2003-06-06 Detection electrochimique de polymorphismes touchant un nucleotide unique (snp)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16559302A 2002-06-07 2002-06-07
US10/165,593 2002-06-07

Publications (2)

Publication Number Publication Date
WO2003104420A2 WO2003104420A2 (fr) 2003-12-18
WO2003104420A3 true WO2003104420A3 (fr) 2004-05-13

Family

ID=29732079

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/018093 WO2003104420A2 (fr) 2002-06-07 2003-06-06 Detection electrochimique de polymorphismes touchant un nucleotide unique (snp)

Country Status (3)

Country Link
EP (1) EP1543153A2 (fr)
AU (1) AU2003251424A1 (fr)
WO (1) WO2003104420A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2543116A1 (fr) * 2003-10-27 2005-05-19 Genelabs Technologies, Inc. Procedes de preparation de derives 7-(2'-.szlig.-d-ribofuranosyl substitue)-4-(nr2r3)-5-(ethyn-1-yl substitue)-pyrrolo[2,3-d]pyrimidine
US20090233280A1 (en) * 2005-12-28 2009-09-17 Canon Kabushiki Kaisha Method of acquiring information regarding base sequence and information reading device for the same
EA027558B1 (ru) * 2011-05-19 2017-08-31 Эйджена Байосайенс, Инк. Способ обнаружения мультиплексных нуклеиновых кислот
CN104232773A (zh) * 2014-06-11 2014-12-24 国家体育总局体育科学研究所 一种用于运动性心脏意外筛查的snp复合检测体系
CN114540470A (zh) 2015-04-24 2022-05-27 基纳生物技术有限公司 用于次要等位基因和多态性的鉴定和定量的多重化方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5608063A (en) * 1986-07-02 1997-03-04 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
US6212093B1 (en) * 2000-01-14 2001-04-03 North Carolina State University High-density non-volatile memory devices incorporating sandwich coordination compounds
US6290839B1 (en) * 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
US6361671B1 (en) * 1999-01-11 2002-03-26 The Regents Of The University Of California Microfabricated capillary electrophoresis chip and method for simultaneously detecting multiple redox labels

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5608063A (en) * 1986-07-02 1997-03-04 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
US6290839B1 (en) * 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
US6361671B1 (en) * 1999-01-11 2002-03-26 The Regents Of The University Of California Microfabricated capillary electrophoresis chip and method for simultaneously detecting multiple redox labels
US6212093B1 (en) * 2000-01-14 2001-04-03 North Carolina State University High-density non-volatile memory devices incorporating sandwich coordination compounds

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
AHERN H.: "Biochemical, reagent kits offer scientists good return on investment", THE SCIENTIST, vol. 9, no. 15, 24 July 1995 (1995-07-24), pages 1 - 5, XP002921157 *

Also Published As

Publication number Publication date
WO2003104420A2 (fr) 2003-12-18
AU2003251424A8 (en) 2003-12-22
EP1543153A2 (fr) 2005-06-22
AU2003251424A1 (en) 2003-12-22

Similar Documents

Publication Publication Date Title
WO2004033629A3 (fr) Transcription inverse sur des microreseaux
WO2005089508A3 (fr) Detection de sequence d'adn par extension limitee d'amorce
WO2003046208A3 (fr) Determination de polypmorphisme parallele par amplification et correction d'erreur
HK1098792A1 (en) Base specific cleavage of methylation-specific amplification products in combination with mass analysis
WO2005044836A3 (fr) Composes nucleotidiques macromoleculaires et leurs procedes d'utilisation
ATE489477T1 (de) Verfahren zum nachweis von sequenzunterschieden
AU2009355009A8 (en) THD primer target detection
DK1330546T3 (da) Fremgangsmåde til amplifikation og eventuel karakterisering af nucleinsyrer
WO2004081186A8 (fr) Polymorphismes genetiques associes a la stenose, procedes de detection et utilisations associees
WO2006026388A3 (fr) Techniques d'amplification d'acide nucleique avec une seule amorce
CA2662837A1 (fr) Procedes et substances destines a isoler et a detecter de petits polynucleotides
SE0202867D0 (sv) New sequencing method
WO2007110656A3 (fr) Procédés et appareil de génotypage
WO2007004057A3 (fr) 4c
EP1256632A3 (fr) Criblage à haut rendement de polymorphismes
AU4228001A (en) Method for detecting cytosine methylation in dna samples
WO2012046981A3 (fr) Détection par pcr en temps réel de polymorphismes nucléotidiques simples
DE502004004202D1 (de) Verfahren zum nachweis von cytosin-methylierungen in dna
DE602004004988D1 (de) Methylierungsstatus-Detektionsassays mittels methylierungsspezifischer Primerextension (MSPE)
WO2003104420A3 (fr) Detection electrochimique de polymorphismes touchant un nucleotide unique (snp)
WO2006076010A3 (fr) Procedes et compositions permettant de detecter des bacteries au moyen d'une pcr multiplex
AU2003288105A1 (en) Microarray-based method for amplifying and detecting nucleic acids during a continuous process
DE69902799D1 (de) Von kreisförmiger Matrize abhängige Kettenreaktion
EP1178119A3 (fr) Procédé multiplex basé sur PCR pour la détermination des haplotypes
ATE455866T1 (de) Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2003757435

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2003757435

Country of ref document: EP

WWR Wipo information: refused in national office

Ref document number: 2003757435

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP

WWW Wipo information: withdrawn in national office

Ref document number: 2003757435

Country of ref document: EP