WO2003095630A3 - Peau bioartificielle - Google Patents

Peau bioartificielle Download PDF

Info

Publication number
WO2003095630A3
WO2003095630A3 PCT/EP2003/004784 EP0304784W WO03095630A3 WO 2003095630 A3 WO2003095630 A3 WO 2003095630A3 EP 0304784 W EP0304784 W EP 0304784W WO 03095630 A3 WO03095630 A3 WO 03095630A3
Authority
WO
WIPO (PCT)
Prior art keywords
skin
genetically modified
protein
fibroblasts
modified fibroblasts
Prior art date
Application number
PCT/EP2003/004784
Other languages
German (de)
English (en)
Other versions
WO2003095630A2 (fr
Inventor
Hans-Guenther Machens
Original Assignee
Hans-Guenther Machens
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hans-Guenther Machens filed Critical Hans-Guenther Machens
Priority to AU2003239844A priority Critical patent/AU2003239844A1/en
Priority to EP03732323A priority patent/EP1504087A2/fr
Publication of WO2003095630A2 publication Critical patent/WO2003095630A2/fr
Publication of WO2003095630A3 publication Critical patent/WO2003095630A3/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/33—Fibroblasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A61K38/185—Nerve growth factor [NGF]; Brain derived neurotrophic factor [BDNF]; Ciliary neurotrophic factor [CNTF]; Glial derived neurotrophic factor [GDNF]; Neurotrophins, e.g. NT-3
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/18—Growth factors; Growth regulators
    • A61K38/1858—Platelet-derived growth factor [PDGF]
    • A61K38/1866—Vascular endothelial growth factor [VEGF]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/39—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/091—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells melanocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts

Abstract

L'invention concerne une peau bioartificielle présentant une matrice poreuse sur laquelle se trouvent des fibroblastes isogènes ou autologues qui expriment au moins une protéine angiogénétique, une protéine neurotrophe et au moins une protéine stabilisant la jonction épidermo-dermale ainsi que la formation d'un épiderme conforme au coloris, un mélange de kératinocytes et mélanocytes isogènes ou autologènes, leur procédé de production et leur utilisation.
PCT/EP2003/004784 2002-05-07 2003-05-07 Peau bioartificielle WO2003095630A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003239844A AU2003239844A1 (en) 2002-05-07 2003-05-07 Bioartificial skin comprising genetically modified fibroblasts
EP03732323A EP1504087A2 (fr) 2002-05-07 2003-05-07 Peau bioartificielle

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10220472A DE10220472A1 (de) 2002-05-07 2002-05-07 Bioartifizielle Haut
DE10220472.1 2002-05-07

Publications (2)

Publication Number Publication Date
WO2003095630A2 WO2003095630A2 (fr) 2003-11-20
WO2003095630A3 true WO2003095630A3 (fr) 2004-03-04

Family

ID=29285189

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/004784 WO2003095630A2 (fr) 2002-05-07 2003-05-07 Peau bioartificielle

Country Status (4)

Country Link
EP (1) EP1504087A2 (fr)
AU (1) AU2003239844A1 (fr)
DE (1) DE10220472A1 (fr)
WO (1) WO2003095630A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2263382B1 (es) 2005-05-16 2007-11-16 Fundacion Para La Investigacion Biomedica Del Hospital Gregorio Marañon Matriz artificial de gel de fibrina endotelizada superproductora de factores proangiogenicos.
CN101955908B (zh) * 2010-07-22 2013-01-23 中国医学科学院皮肤病研究所 用于检测物质致敏性和药物筛选的人工表皮体外模型

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512475A (en) * 1986-04-18 1996-04-30 Advanced Tissue Sciences, Inc. Three-dimensional skin cell and tissue culture system
WO1999045770A1 (fr) * 1998-03-09 1999-09-16 The Board Of Trustees Of The Leland Stanford Junior University Equivalent de peau et procedes de formation et d'utilisation
WO2000029553A1 (fr) * 1998-11-19 2000-05-25 Organogenesis Inc. Constructions tissulaires transgeniques et procedes de production et d'utilisation associes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512475A (en) * 1986-04-18 1996-04-30 Advanced Tissue Sciences, Inc. Three-dimensional skin cell and tissue culture system
WO1999045770A1 (fr) * 1998-03-09 1999-09-16 The Board Of Trustees Of The Leland Stanford Junior University Equivalent de peau et procedes de formation et d'utilisation
WO2000029553A1 (fr) * 1998-11-19 2000-05-25 Organogenesis Inc. Constructions tissulaires transgeniques et procedes de production et d'utilisation associes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
MACHENS, H.-G. ET AL.: "Bioartificial skin", CELLS TISSUES ORGANS, vol. 167, no. 2-3, 2000, pages 88 - 94, XP008022499 *
MACHENS, H.-G. ET AL.: "Gentherapeutische Möglichkeiten in der plastischen Chirurgie", DER CHIRURG, vol. 71, no. 2, February 2000 (2000-02-01), pages 152 - 158, XP002256227 *
MACHENS, H.-G. ET AL.: "Platelet-derived growth factor-AA-mediated functional angiogenesis in the rat epigastric island flap after genetic modification of fibroblasts is ischemia dependent", SURGERY, vol. 131, no. 4, April 2002 (2002-04-01), pages 393 - 400, XP008018381 *
SUPP, D.M. ET AL.: "Genetic modification of cultureed skin substitutes by transduction of human keratinocytes and fibroblasts with platelet-derived growth factor-A", WOUND REPAIR AND REGENERATION, vol. 8, no. 1, 2000, pages 26 - 35, XP001154286 *

Also Published As

Publication number Publication date
AU2003239844A1 (en) 2003-11-11
WO2003095630A2 (fr) 2003-11-20
AU2003239844A8 (en) 2003-11-11
EP1504087A2 (fr) 2005-02-09
DE10220472A1 (de) 2003-11-27

Similar Documents

Publication Publication Date Title
AU2002356861A1 (en) Fibrin membrane and methods for its preparation- application to artificial skin
DE69925553D1 (de) Tintenzusammensetzung zur erzeugung von lichtbeständigen bildern sowie tintensortiment
ATE247099T1 (de) Sulfonamid-derivate, verfahren zur ihrer herstellung und ihre verwendung als arzneimittel
ATE250399T1 (de) Gebrauch von autologen, dermalen fibroblasten zur reperatur von haut- oder weichgewebeschäden
DE60011243D1 (de) Verfahren zur herstellung von mit antioxidant gedoptem uhmwpe und daraus hergestelltes implantat
WO2001034646A3 (fr) Gelatines de recombinaison
ATE410998T1 (de) Verwendung einer proteinkinase a-inaktivierenden verbindung in einer kosmetisch verträglichen zusammensetzung zur aufhellung der haut
DE59807213D1 (de) 11beta-halogen-7alpha-substituierte-estratriene, verfahren zur herstellung pharmazeutischer präparate, die diese 11beta-halogen-7alpha-substituierte-estratriene enthalten, sowie deren verwendung zur herstellung von arzneimitteln
DE69729864D1 (de) Herstellung von laktamen aus aliphatischen alpha, omega-dinitrilen
DE69712610D1 (de) Erythramicin-derivate, ihre verfahren zur herstellung und ihre verwendung als arzneimittel
WO2007062386A3 (fr) Greffon de follicule capillaire issu de peau construite par genie tissulaire
DE69715210T2 (de) Modifiziertes mcm-56, seine herstellung und anwendung
TW200611711A (en) Cosmetic treatment system and methods
WO2001040177A3 (fr) Esters de vitamine d3 et utilisation de ces ceux-ci
DE69734464D1 (de) Verfahren zur expression und herstellung von rekombinantem, menschlichem beta-cis-interferon
DE68917154T2 (de) C-terminus-amidierungsenzym-zusammensetzung, verfahren zur herstellung und verwendung.
WO2002050111A3 (fr) Laminine 10 isolee
WO2003095630A3 (fr) Peau bioartificielle
WO2000066731A3 (fr) Laminine 5 recombinee
WO2001064837A8 (fr) NETRINE β ET SES UTILISATIONS
WO2000066732A3 (fr) Laminine 8 et ses procedes d'utilisation
TW200716979A (en) Screening methods for compounds that affect melanogenesis
DK0939769T3 (da) Vertebrat-Smoothened-proteiner
ATE550345T1 (de) Sfrp, mit sfrp-interagierende peptidmotive sowie verwendungsverfahren
DE69820078D1 (de) Druckfosgenierung von alkoholen zur herstellung von chloroformiaten

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2003732323

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2003732323

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP

WWW Wipo information: withdrawn in national office

Ref document number: 2003732323

Country of ref document: EP