WO2003087344A1 - Nouvelle souches capables de produire un acide linoleique conjugue, composition encapsulee comprenant cet acide et procedes de fabrication correspondant - Google Patents

Nouvelle souches capables de produire un acide linoleique conjugue, composition encapsulee comprenant cet acide et procedes de fabrication correspondant Download PDF

Info

Publication number
WO2003087344A1
WO2003087344A1 PCT/KR2003/000742 KR0300742W WO03087344A1 WO 2003087344 A1 WO2003087344 A1 WO 2003087344A1 KR 0300742 W KR0300742 W KR 0300742W WO 03087344 A1 WO03087344 A1 WO 03087344A1
Authority
WO
WIPO (PCT)
Prior art keywords
cla
strain
strains
cbg
present
Prior art date
Application number
PCT/KR2003/000742
Other languages
English (en)
Inventor
So-Mi Kim
Deok-Kun Oh
Dae-Heoun Baek
Hong-Sig Sin
Si-Ho Park
Yu-Jin Lee
Soo-Jong Um
Young-Soy Rho
Jong-Sup Park
Dong-Myong Kim
Original Assignee
Chebigen Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chebigen Inc. filed Critical Chebigen Inc.
Priority to AU2003221135A priority Critical patent/AU2003221135A1/en
Priority to JP2003584288A priority patent/JP4108613B2/ja
Publication of WO2003087344A1 publication Critical patent/WO2003087344A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04B—TRANSMISSION
    • H04B3/00—Line transmission systems
    • H04B3/54—Systems for transmission via power distribution lines
    • H04B3/56—Circuits for coupling, blocking, or by-passing of signals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C11/00—Milk substitutes, e.g. coffee whitener compositions
    • A23C11/02—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
    • A23C11/10—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
    • A23C11/103—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins containing only proteins from pulses, oilseeds or nuts, e.g. nut milk
    • A23C11/106—Addition of, or treatment with, microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1234—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/60—Drinks from legumes, e.g. lupine drinks
    • A23L11/65—Soy drinks
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12—Fatty acids or derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/30—Encapsulation of particles, e.g. foodstuff additives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/745—Bifidobacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06Q—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES; SYSTEMS OR METHODS SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES, NOT OTHERWISE PROVIDED FOR
    • G06Q20/00—Payment architectures, schemes or protocols
    • G06Q20/04—Payment circuits
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06Q—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES; SYSTEMS OR METHODS SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES, NOT OTHERWISE PROVIDED FOR
    • G06Q20/00—Payment architectures, schemes or protocols
    • G06Q20/08—Payment architectures
    • G06Q20/20—Point-of-sale [POS] network systems
    • G06Q20/202—Interconnection or interaction of plural electronic cash registers [ECR] or to host computer, e.g. network details, transfer of information from host to ECR or from ECR to ECR
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06Q—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES; SYSTEMS OR METHODS SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES, NOT OTHERWISE PROVIDED FOR
    • G06Q20/00—Payment architectures, schemes or protocols
    • G06Q20/30—Payment architectures, schemes or protocols characterised by the use of specific devices or networks
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06Q—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES; SYSTEMS OR METHODS SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES, NOT OTHERWISE PROVIDED FOR
    • G06Q20/00—Payment architectures, schemes or protocols
    • G06Q20/30—Payment architectures, schemes or protocols characterised by the use of specific devices or networks
    • G06Q20/34—Payment architectures, schemes or protocols characterised by the use of specific devices or networks using cards, e.g. integrated circuit [IC] cards or magnetic cards
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/535—Pseudocatenulatum
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/13—Brevibacterium
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04B—TRANSMISSION
    • H04B2203/00—Indexing scheme relating to line transmission systems
    • H04B2203/54—Aspects of powerline communications not already covered by H04B3/54 and its subgroups
    • H04B2203/5429—Applications for powerline communications

Definitions

  • the present invention relates to novel strains capable of producing conjugated linoleic acid (hereinafter referred to as CLA).
  • CLA which is a conjugated isomer of linoleic acid (hereinafter referred to as
  • LA an essential fatty acid
  • an essential fatty acid is a natural fatty acid found in a small amount in milk or muscle of ruminants.
  • CLA has conjugated double bonds at positions cis-9 and trans-11 or at positions trans-10 and cis-12, in intra and trans configuration. Particularly, by a conjugated double bond at cis-9 and trans-11 positions, physiological activities useful to human bodies are expressed.
  • CLA is known to show reduction in development of the artery sclerosis
  • CLA may be usefully used as an effective ingredient of functional food and medicaments.
  • CLA is mainly contained in animal food, particularly at a large amount in ruminant animals. It is shown that beef contains 2.9 ⁇ 4.3 mg CLA/fat, lamb meat contains 5.6 mg CLA/fat and marine products contain as little as 0.3-0.6 mg CLA/fat. For dairy products, milk contains 5.5 mg CLA/fat and cheese contains 3 ⁇ 7 rag CLA/fat. With respect to human beings' daily CLA intake, it is presumed that oriental people who principally keep a vegetable diet intake 0.1 g of CLA per day and western people who eat more meat intake 0.4 g of CLA per day.
  • the conventional methods include methods for chemically synthesizing CLA from LA, such as urea addition, molecular distillation, HPLC, etc., and there are several isolated microorganism able to convert LA into CLA.
  • the chemical synthesis methods have problems, such that they require expensive equipments or too much time is taken for processes.
  • conventional chemical synthesis methods produce a kind of CLA along with various kinds of isomers, it is very inefficient to perform production of a kind of CLA by such chemical synthesis methods. Therefore, the most efficient method for producing CLA is to produce CLA by a microorganism, followed by isolation.
  • Representative microorganisms capable of producing CLA includes microorganisms in the bowels such as Lacto bacillus, Propionibacterium, Butyrivibrio fibrisolvens etc. and are usefully used as an effective ingredient in functional food or medicaments such as probiotics and feed which are fed to animals in many countries.
  • Korean Patent Application No. 10-2001-0047292 disclosed a method for adding pure cis-9 and trans-11 type CLA which is synthesized by a microorganism of Lactobacillus genus to food. However, only an infinitesimal amount of CLA was found in an actual product.
  • a CLA producing microorganism in order to be used as an effective ingredient in food or medicaments, it should survive at a high level similar to that upon production while the product is distributed and also should maintain a high viability and activity in the stomach and bowels after intake into a body of an animal including human beings. In addition, it should be excellent in resistance to antibiotics to hold a predominant position in the competition with harmful bacteria in the bowels, including superbacteria.
  • the present invention has been made in view of the above problems, and it is an object of the present invention to provide novel strains which can produce conjugated linoleic acid, have excellent resistance to acids and antibiotics and can indirectly produce CLA when added to food and medicaments.
  • the above and other objects can be accomplished by the provision of novel strains capable of producing CLA.
  • the strains are characterized in that they are isolated from feces of infants and they can convert LA to CLA.
  • the strains include Bifidobacterium breve CBG-C2, Bifidobacterium pseudocartenulatum CBG-C4 and Enterococcus faecium CBG-C5.
  • strains can be isolated as follow:
  • Strains are isolated from feces of Korean infants and randomly selected 300 colonies are cultured in a medium with LA as a substrate. The medium is extracted with hexane and the extracts were measured for their absorbance to screen strains which have excellent CLA productivity.
  • the strains screened by the above procedures were once again examined whether they can produce CLA from LA, by subjecting fatty acids produced by the strains to gas chromatography (GC).
  • GC gas chromatography
  • the strains thus isolated were three types, designated CBG-C2, CBG-C4 and CBG-C5, respectively and were deposited at Korean Agricultural Culture Collection in the National Institute of Agricultural Biotechnology under Accession numbers KACC 91001, KACC 91003 and KACC 91002, respectively, on April 3, 2002. Also, CBG-C2 (KCTC 10462BP) and CBG-C4 (KCTC 10208BP) were deposited at the Korean Collection for Type Cultures (KCTC) in Korea Research Institute of Bioscience and Biotechnology on March 25, 2002 and April 10, 2003, respectively.
  • the strains obtained by the above-described method are excellent in CLA productivity and have strong resistance to acids such as stomach acid and bile, and antibiotics.
  • CLA produced by microorganisms according to the present invention comprises only the stereostructures of cis-9 and trans-11, which are isomers having various physiological activities, as conventionally known to the art.
  • Fatty acids and acyglycerol containing these structures can be widely used for development of dairy products from various animals, dairy products from vegetable materials, fermented food and functional health food, probiotics and the like. Therefore, the strains according to the present invention can be effectively used in biosynthesis of isomers of CLA by biological methods such as immobilization.
  • the strains according to the present invention can be added to food and medicaments as not only a live strain but also a killed strain. This is because the strains according to the present invention can release CLA to a culture fluid or reaction fluid while accumulating a large amount of CLA in the strains.
  • the strain which are cultured in a medium containing LA may be added as an effective ingredient to various compositions such as food and medicaments so that CLA is indirectly produced and it is thus possible to promote development of functional products containing CLA in a large amount by resolve the conventional problems in that CLA cannot be directly added to food and medicaments.
  • the present invention provides a food or pharmaceutical composition containing the strain.
  • the compositions according to the present invention contain at least one selected from Bifidobacterium breve CBG-C2, Bifidobacterium pseudocartenulatum CBG-C4 and Enterococcus faecium CBG-C5 as an effective ingredient.
  • the composition may comprise an additional effective ingredient such as an organic acid to enhance CLA production rate and improve growth stability of strain.
  • an organic acid in the composition according to the present invention may be CLA which is chemically synthesized or is isolated and purified from microorganisms.
  • composition according to the present invention may comprise any one selected from the strains according to the present invention and CLA at the same time.
  • the composition according to the present invention may further contain various adjuvant components in addition to the effective ingredient, when needed.
  • the food composition according to the present invention may comprise vitamins such as vitamin A, vitamin Bl, vitamin B2, vitamin B3, vitamin B6 and vitamin B12, folic acid, vitamin C, vitamin D3, vitamin E and the like, minerals such as copper, calcium, iron, magnesium, potassium, zinc and the like, or lactic acid bacteria and the like.
  • a heath drink composition may comprise an additional component such as a flavor or natural carbohydrates, like other drinks.
  • the flavor includes natural sweetening agents such as thaumatin and stevia extract and synthetic sweetening agents such as saccharin and aspartame.
  • the natural carbohydrate includes monosaccharides such as glucose, fructose and the like, disaccharides such as maltose, sucrose and the like, polysaccharides such as dextrin, cyclodextrin and the like, and sucrose alcohols such as xylitol, sorbitol, erythritol and the like.
  • the usage or intake of the pharmaceutical composition according to the present invention is preferably 60 to 130 uM (Cancer Epidemiol. Biol. Prev. 2000. 9:689-696) and the usage or intake of the food composition according to the present invention is preferably 3.4 to 6 g/day (J. Nutr. 2000. 130:2943-2948), though it may be adjusted, as needed.
  • the composition is stably absorbed into a body regardless of the intake.
  • the usage or intake can be adjusted according to various factors including the type and amount of an effective ingredient and other components contained in the composition, formulation type and age, body weight, general health condition, sex and diet of a patient, administration time, administration route and release rate of the composition, treatment duration, simultaneously used drugs.
  • the composition Upon administration to a human body, the composition would not show side effects, as compared to conventional food and medicaments containing chemically synthesized CLA.
  • the composition may be formulated by combining at least one pharmaceutically acceptable or edible carrier with the effective ingredient.
  • the pharmaceutically acceptable or edible carrier which can be used in the present invention includes a saline solution, sterile water, Ringer's solution, glucose solution, maltodextrin solution, glycerol, ethanol and a mixture of one or more thereof and may be combined with a conventional additive such as an antioxidant, a buffering agent, a bacteriostatic agent and the like, when needed.
  • the composition can be formulated into injections such as aqueous solutions, suspensions, emulsions, pills, capsules, granules or tablets by further adding a diluting agent, a dispersing agent, a surfactant, a binder and a lubricant.
  • the composition may be preferably formulated by a proper method known to the art, or a method described in Remington's Pharmaceutical Science (the latest), Mack Publishing Company, Easton PA, according to diseases or components.
  • composition according to the present invention can be formulated in the form of granules, powders, coated tablets, tablets, capsules, liquids and solutions, extracts, suppositories, syrups, juices, suspensions, emulsions, release-sustained formulation of an active compound and the like.
  • a capsule formulation of the composition According to the present invention, there is provided a capsule formulation of the composition.
  • the capsule formulation comprises a coating material and a core material enclosed in the coating material.
  • the core material of the capsule formulation comprises preferably both any one selected from the strains according to the present invention and CLA.
  • water- soluble polysaccharides which are excellent in absorption, dispersion and adhesion are usable.
  • the water-soluble polysaccharides which can be used in the present invention is at least one selected from the group consisting of starch, agar, carageenan, alginic acid, sodium alginate, polymethacrylate, wheat protein, soy protein, cellulose derivatives such as methylcellulose, hydroxylpropylcellulose, hydroxyl-propylmethylcellulose and the like; gums such as xanthan gum, arabic gum, locust bean gum, guar gum, tamarind gum, tara gum, karaya gum, tragacanth gum, ghatti gum and the like; and gellan, xanthan, pectin (LM, HM type, dextran, glucan, glucomannan, arabino galactan, furcelleran, pullulan, glucosamine, gelatin and casein.
  • starch agar, carageenan, alginic acid, sodium alginate, polymethacrylate, wheat protein, soy protein, cellulose derivatives
  • the amount of the coating material can be adjusted according to the final use and purpose and is preferably 1 to 80 % by weight based on the weight of the core.
  • the capsule may further comprise substances which are commonly used in a coating material to improve release control effect or to increase solubility.
  • the capsule may further comprise an emulsifying agent, a protective agent and plasticizer, as needed.
  • the capsule formulation may be produced by using one of methods which are commonly used for encapsulation.
  • a method for preparing the capsule according to the present invention is performed by a typical encapsulation method, which comprises a process for emulsification, in which strains which are obtained by culturing the strain according to the present invention, an organic acid and a coating material for capsule are dispersed in a solvent with emulsion stability, a process for production of capsule membrane, in which the emulsified dispersion is stirred to form capsule membrane, and a process for curing, in which a curing agent and a reagent are added to cure the capsule membrane.
  • the capsule formulation thus obtained can protect the strain from outer circumstances by antioxidation and thus, can be stored stably at a low temperature for a long period of time.
  • fatty acids such as CLA are unstable to heat, enzymes, acids, alkalis, microorganisms and the like.
  • microencapsulation by a proper coating material may improve storage stability and convenience for internal use.
  • the capsule formulation maintains a live strain number at a uniform level in the bowels under acid conditions by regular release control.
  • the capsule fonnulation can increase specific gravity and dispersibility in the aqueous phase of the body by microencapsulation to further enhance the bioavailability, thereby improving applicability to preserved food, milk and drinks, and dairy products.
  • the capsule formulation allows the strains according to the present invention to grow stably in the bowels.
  • the strains which have been grown in the bowels can continuously produce CLA, whereby it is possible to provide inhibition of development of the artery sclerosis, reduction of body fat, improvement of immunity, anticancer effect, growth promoting effect and the like.
  • the strains according to the present invention hold dominant species in the bowels, thereby acting as probiotics to inhibit growth of harmful microorganisms in the bowels. Therefore, it is possible to continuously activate and improve the bowel function.
  • composition comprising the capsule formulation includes, for example, dairy products (milk, soy milk, processed milk), fermented milk (liquid type yogurt, curd type yogurt), fermented food (Kimchii, soy and bean paste), animal feed, health supplementary food and the like.
  • Food compositions containing the strains according to the present invention as an effective ingredient include animal feed, fermented food such as various kinds of Kimchii, soy and bean pastes and fermented dairy products such as yogurt and cheese and can be effectively expected to prevent cancers, to enhance immunity and to reduce body fat.
  • compositions containing the strains according to the present invention as effective ingredients can be used in the prevention and treatment of diseases inhibited by CLA, such as cancers, artery sclerosis, diabetes and obesity.
  • Fig. 1 is a view showing the result of a HPLC assay to confirm the element composition of fatty acids produced by the strains according to the present invention
  • Fig. 2 is a view showing the growth conditions of the strains according to the present invention in a medium with linoleic acid (LA) added, the CLA production and the change in pH of the medium;
  • LA linoleic acid
  • Fig. 3 is a view showing the growth conditions of the strains according to the present invention in a medium without linoleic acid (LA) and the CLA production;
  • Fig. 4 is a view for comparison of the amounts of CLA distributed in the medium and the strains, respectively, when the strains according to the present invention are cultured in a medium with linoleic acid (LA) added;
  • LA linoleic acid
  • Fig. 5 is a view for comparison of the amounts of CLA distributed in the medium and the strains, respectively, when the strains according to the present invention are cultured in a medium without linoleic acid (LA); and
  • Fig. 6 is a view showing the result of measuring the growth level (a) and CLA production (b) of the strains according to the present invention which have been cultured in media, to which glucose, fructose, lactose and sucrose, respectively, were added as a carbon source. Examples
  • Example 1 Isolation, identification and characterization of strain
  • Feces of infants were collected to isolate the strains according to the present invention.
  • LA was well emulsified in Tween 80 (0.5 %, w/v), filtered through a cotton filter and added to the MRS medium.
  • Tween 80 0.5 %, w/v
  • the sterilized culture vessel was filled with the MRS medium without any room. About 300 colonies were randomly selected from the cultured medium.
  • the culture fluid was extracted with hexane and the extract was measured for absorbance at 233 nm to determine the amount of produced CLA, which was then con"ected by the amount of strain, that is, the absorbance at 600 nm to compare the relative amount of produced CLA.
  • the measurement of the live strain number was performed by plate- culturing in the MRS agar medium by 10 times dilution, placing the medium in an anaerobic tank (Difco, USA) with a gas pack for 48 hours and thereafter, counting the number of produced colonies.
  • CBG-C2 three strains which were excellent in the CLA productivity were selected and designated CBG-C2, CBG-C4 and CBG-C5, respectively.
  • the strains were identified for their genus and species by analysis of their 16S rRNA sequence.
  • the CBG-C2 strain belongs to Bifidobacterium breve
  • the CBG-C4 strain belongs to Bifidobacterium pseudocartenulatum
  • the CBG-C5 strain belongs to Enterococcus faecium.
  • CBG-C2 Korean Agricultural Culture Collection in the National Institute of Agricultural Biotechnology under Accession numbers KACC 91001 (CBG-C2), KACC 91003 (CBG-C4) and KACC 91002 (CBG-C5), respectively, on April 3, 2002.
  • CBG-C2 KCTC 10462BP
  • CBG- C4 KCTC 10208BP
  • Each strain was cultured in a medium containing LA (500 ⁇ glml) for 48 hours and centrifuged.
  • the strain was suspended in a medium or distilled water, mixed with isopropyl alcohol in a volume of twice the volume of the strain and the vigorously stirred. Then, a 1.5 volume of hexane was added and thoroughly mixed for 3 minutes by shaking. The resulting mixture was centrifuged at 3000 rpm for 5 minutes and the supernatant was measured for absorbance at 233 nm.
  • the extracted fatty acids were methylesterified according to the method described in American Oil Chemists's Society: Official Method and Recommended Practoces pf AOCS, 4th. ed.(1989) to form a sample for the GC analysis.
  • the conditions of GC were as follows: the GC DS-6200 (DONAM) with
  • the column was HP-FFAP capillary column (30m ⁇ 0.25mm, thickness 0.25/.H1), the oven temperature was 210 ° C , the injector temperature was 250 ° C and the detector temperature was 270 ° C .
  • the carrier gas was helium and eluted at a flow rate of 1 mi/min, and the split ratio was 50:1.
  • the peak areas were determined using an integrating meter (Model 3390A, Hewlett-packard, USA) equipped to the apparatus.
  • the identification of CLA was performed through comparison with the retention time of a reference material and the content of CLA was determined by the ratio of the area of CLA to the area of a reference material. The results are shown in Fig. 1. As shown in Fig. 1, all the strains showed LA peaks and CLA peaks.
  • strains according to the present invention could produce CLA using LA as a substrate.
  • the strains showed increase in the amount of produced CLA when entering to the log phase and produced the highest amount of CLA just prior to the stationary phase.
  • the pHs of the media were about 6.5 at the initial stage and drops as the time went on, whereby it was about 4.2 at about 50 hours.
  • each strain which had been activated by pre-cultivation was inoculated at 1% into the MRS medium without LA, dispensed in a 20 mi test tube and cultured. During cultivation, a growth curve was made and from 18 hours after beginning the cultivation, every 6 to 12 hours, the pH of the medium was measured.
  • the strain was collected and suspended with a Tris-HCl buffer solution in a volume of 10 times the volume of the strain and subjected to an enzyme reaction to examine the change in the factor of CLA isomerase according to the change of the growth curve.
  • LA was added to the enzyme reaction at a concentration of 10O ⁇ g/mi.
  • results obtained from the case where the strains were cultured in media containing and the results obtained from the case where the strains were cultured in media without containing LA are useful to understand the growth stage of the strains showing the highest growth factor per unit strain in development of products by fermentation of microorganisms in the presence of a substrate and in indirect production CLA by adding the strains as an effective ingredient, respectively.
  • each strain was inoculated into a medium containing LA and measured for the strain level and CLA production at 18, 24, 36 and 48 hours. The results are shown in Fig. 4.
  • each strain was inoculated into a medium without LA and cultured for 18, 24, 36 and 48 hours. After cultivation, the strain was collected mixed with a buffer solution containing LA. The mixture solution was left for 1 hour for reaction and measured for the distribution of produced CLA. The results are shown in Fig. 5.
  • strains according to the present invention can secret the produced CLA to a medium while accumulating in their body.
  • strains are useful to be added as an effective ingredient to food and medicaments for indirect production of CLA.
  • a carbon source which is the most suitable for each strain is glucose for CBG-C2 and lactose and sucrose for CBG-C4, and when the medium does not contain LA, it is lactose for CBG-C2 and lactose and sucrose for CBG-C4.
  • CBG-C2 and CBG-C4 had the highest CLA production when using glucose as a carbon source while CBG-C5 was not affected by the carbon source.
  • the pH resistance, bile salt resistance and antibiotic resistance of the strains according to the present invention were confirmed as follows. In order to examine the use of the novel strains which had been isolated and identified according to the present invention in application to products fermented by inoculating the CLA producing lactic acid bacteria or additives using CLA contained in the strains, growth features of the strains were compared.
  • the strains were cultured in a medium containing LA and a medium without containing LA and the change of each medium in pH was observed. The results are shown in Fig. 2 and Fig.3.
  • the strain according to the present invention could grow and propagate at pH in the range of 3.0 to 6.0 and particularly, the CBG-C4 strain showed the most excellent pH resistance.
  • strains according to the present invention can grow and propagate in a wide range from weak alkalinity to weak acidity, particularly in the stomach under acidic conditions resulting from the secretion of stomach acid.
  • the strains could satisfactorily grow and propagate to the bile concentration of 1000 ⁇ g/ i, whereas the control of Bacillus subtilis could no grow even at 100 ⁇ g/mt. Therefore, it was noted that the strain according to the present invention could stably grow and propagate in the gastrointestinal tract where the bile is secreted.
  • the strains which had been cultured overnight in a 20 mi test tube were diluted in the MRS medium to form a solid medium. Lactobacillus reuteri was used as control. Antibiotics used in this experiment were ampicillin, tetracycline, streptomycin, rifamycin and kanamycin, each of which was prepared at concentrations of 50 ⁇ g/mi, 1000 ⁇ g/mi and 10000 ⁇ g/ml.
  • the strains according to the present invention showed resistance to total 2 or 3 antibiotics.
  • CBG-C4 shows most excellent resistance to antibiotics at high levels, such as kanamycin at 1000 ⁇ g/mi and tetracycline at 10000 ⁇ g/mi.
  • strains according to the present invention have resistance to various kinds of antibiotics at a wide range of concentration.
  • Capsules and dairy products containing the strains according to the present invention were prepared as follows.
  • a coating material for microencapsulation of conjugated linoleic acid a mixture comprising 4 types of vegetable polysaccharides, gellan, xanthan, starch and agar, in a concentration of 1 to 5% (w/v) was prepared.
  • Sorbitan monostearate having a HLB (hydrophilic lipophilic balance) value of 4.7 as an emulsifying agent was added to the mixture, heated at 60 ° C so that it was completely dissolved, sterilized by heating and cooled down to 40 ° C to form an aqueous mixture solution.
  • sorbitan monostearate was treated at a concentration of 0.01 to 1% (w/v) and the coating material and the strain was mixed in a ratio of 7:3(w/w) and homogenized.
  • the homogenized strain suspension was treated with cooled water at 10 °C by spraying to form microcapsules.
  • the microcapsules were added in an amount of 1% (w/v) to prepare Kimchii which is one of the low-temperature fermented products.
  • the microcapsules were added in an amount of 4.2% (w/v) and for curd type yogurt and fermented soy milk, the microcapsules were added in an amount of 7.3% (w/v).
  • Each product was stored in a refrigerator kept at a low temperature of 4 ° C or 10 ° C .
  • the dairy products prepared as described above were subjected to an experiment to examine the preservation of microcapsules. As a result, it was shown that when stored in a refrigerator at 4 ° C and 10 ° C for 7 to 14 days, strains were reduced by about lO 1""2 strains/mi. Such reduction rate is very insignificant, judging from a common basis.
  • the capsules according to the present invention could stably maintain the number of strains even when stored for a long period of time and have excellent storage stability.
  • the strains according to the present invention can produce CLA from LA at a high efficiency and show outstanding resistance to stomach acids, bile salts, and antibiotics.
  • strains according to the present invention can accumulate CLA in their body after production and thus have effect to make CLA indirectly produced.
  • composition comprising the strain according to the present invention may be prepared in the form of a capsule comprising the strain according to the present invention in a coating material comprising a water-soluble polysaccharide and be usefully used in functional food and medicaments.
  • strains according to the present invention can be effectively used to biosynthesize not only CLA but also isomers of CLA in large quantities by a biological method such as immobilization.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Business, Economics & Management (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Genetics & Genomics (AREA)
  • Accounting & Taxation (AREA)
  • Biotechnology (AREA)
  • Nutrition Science (AREA)
  • General Physics & Mathematics (AREA)
  • Theoretical Computer Science (AREA)
  • General Business, Economics & Management (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Strategic Management (AREA)
  • Diabetes (AREA)
  • General Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Agronomy & Crop Science (AREA)
  • Botany (AREA)
  • Computer Networks & Wireless Communication (AREA)
  • Molecular Biology (AREA)
  • Epidemiology (AREA)

Abstract

L'invention concerne de nouvelles souches capables de produire un acide linoléique conjugué (CLA). Ces souches comprennent Bifidobacterium breve CBG-C2, Bifidobacterium pseudocartenulatum CBG-C4 et Enterococcus faecium CBG-C5. Ces souches ont une excellente production de CLA et sont capables de sécréter le CLA produit dans un milieu ou de l'accumuler dans des corps. Les souches en question manifestent une forte résistance aux antibiotiques ou à des acides tels que le suc gastrique ou le sel biliaire. Une composition contenant la souche se présente sous la forme d'une capsule, et le CLA encapsulé dans un matériau de revêtement comprend des polysaccharides hydrosolubles et peut s'utiliser dans des aliments ou médicaments fonctionnels.
PCT/KR2003/000742 2002-04-12 2003-04-12 Nouvelle souches capables de produire un acide linoleique conjugue, composition encapsulee comprenant cet acide et procedes de fabrication correspondant WO2003087344A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003221135A AU2003221135A1 (en) 2002-04-12 2003-04-12 New strains capable of producing conjugated linoleic acid, capsulated composition comprising them, and the preparation methods thereof
JP2003584288A JP4108613B2 (ja) 2002-04-12 2003-04-12 共役リノール酸を生産する新規の菌主、これを含有するカプセル剤及びその製造方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2002-0020057 2002-04-12
KR20020020057 2002-04-12

Publications (1)

Publication Number Publication Date
WO2003087344A1 true WO2003087344A1 (fr) 2003-10-23

Family

ID=29244725

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2003/000742 WO2003087344A1 (fr) 2002-04-12 2003-04-12 Nouvelle souches capables de produire un acide linoleique conjugue, composition encapsulee comprenant cet acide et procedes de fabrication correspondant

Country Status (4)

Country Link
JP (1) JP4108613B2 (fr)
KR (3) KR100516377B1 (fr)
AU (1) AU2003221135A1 (fr)
WO (1) WO2003087344A1 (fr)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1500706A1 (fr) * 2002-04-12 2005-01-26 Kabushiki Kaisha Yakult Honsha Procede de production d'acide gras conjugue et aliment /boisson obtenus grace a ce procede
WO2006070891A1 (fr) * 2004-12-28 2006-07-06 Meiji Seika Kaisha, Ltd. Nouvelle souche conferant des proprietes therapeutiques a des compositions de cellules hotes et bacteriennes
EP1789531A1 (fr) * 2004-08-16 2007-05-30 PL Bio Co., Ltd. Lactobacillus rhamnosus a activite de reduction de graisse corporelle et aliments les contenant
JP2008511312A (ja) * 2004-09-02 2008-04-17 ピーエル バイオ カンパニー リミテッド 体脂肪低下機能性ラクトバシラスプランタルムとこれを含有した食品（ｌａｃｔｏｂａｓｉｌｌｕｓｐｌａｎｔａｒｕｍｗｉｔｈｂｏｄｙ−ｆａｔｒｅｄｕｃｉｎｇａｃｔｉｖｉｔｙａｎｄｔｈｅｆｏｏｄｓｃｏｎｔａｉｎｉｎｇｔｈｅｍ）
EP1974734A1 (fr) * 2007-03-28 2008-10-01 Nestec S.A. Probiotiques pour la réduction du risque d'obésité
WO2009043856A2 (fr) * 2007-10-01 2009-04-09 University College Cork, National University Of Ireland, Cork Modulation de la composition en acides gras tissulaires d'un hôte par des bactéries de l'intestin humain
US8497114B2 (en) 2009-09-17 2013-07-30 Morinaga Milk Industry Co., Ltd. Anti-obesity agent, anti-obesity food or beverage, glucose tolerance-ameliorating agent, and food or beverage for amelioration of glucose tolerance
CN103981117A (zh) * 2013-12-24 2014-08-13 北京伟嘉人生物技术有限公司 一株高抗逆性屎肠球菌及其培养方法和应用
KR101540743B1 (ko) * 2014-04-28 2015-08-03 대한민국 공액리놀레산의 생산방법 및 콩 발효식품

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101156340B1 (ko) * 2009-06-17 2012-06-13 고려대학교 산학협력단 비피도박테리움 브레베 ｌｍｃ５２０ 균주를 이용한 공액리놀렌산의 생산방법
KR101446309B1 (ko) 2012-07-06 2014-10-07 (주)케비젠 공액리놀레산을 생산하는 비피도박테리움 애니말리스 균주 및 그 용도
CN105925514B (zh) * 2016-07-12 2019-04-09 江南大学 一株短双歧杆菌及其制备共轭亚油酸或共轭亚麻酸的应用
CN114317320B (zh) 2020-08-24 2022-12-27 汤臣倍健股份有限公司 一种短双歧杆菌207-1及其应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5674901A (en) * 1995-06-01 1997-10-07 Wisconsin Alumni Research Foundation Methods of treating animals to maintain or increase CD-4 and CD-8 cell populations
US5760082A (en) * 1994-08-29 1998-06-02 Wisconsin Alumni Research Foundation Dietetic foods containing conjugated linoleic acids
US6060304A (en) * 1995-06-01 2000-05-09 Wisconsin Alumni Research Foundation Method of producing conjugated fatty acids
US6242621B1 (en) * 1998-05-04 2001-06-05 Conlinco., Inc. Isomer enriched conjugated linoleic acid compositions
US6342619B2 (en) * 1999-04-01 2002-01-29 Michael C. Seidel Synthesis of conjugated fatty acid

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5760082A (en) * 1994-08-29 1998-06-02 Wisconsin Alumni Research Foundation Dietetic foods containing conjugated linoleic acids
US5760082C1 (en) * 1994-08-29 2001-03-06 Wisconsin Alumni Res Found Dietetic foods containing conjugated linoleic acids
US5674901A (en) * 1995-06-01 1997-10-07 Wisconsin Alumni Research Foundation Methods of treating animals to maintain or increase CD-4 and CD-8 cell populations
US6060304A (en) * 1995-06-01 2000-05-09 Wisconsin Alumni Research Foundation Method of producing conjugated fatty acids
US6242621B1 (en) * 1998-05-04 2001-06-05 Conlinco., Inc. Isomer enriched conjugated linoleic acid compositions
US6342619B2 (en) * 1999-04-01 2002-01-29 Michael C. Seidel Synthesis of conjugated fatty acid

Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1500706A4 (fr) * 2002-04-12 2005-08-03 Yakult Honsha Kk Procede de production d'acide gras conjugue et aliment /boisson obtenus grace a ce procede
EP1500706A1 (fr) * 2002-04-12 2005-01-26 Kabushiki Kaisha Yakult Honsha Procede de production d'acide gras conjugue et aliment /boisson obtenus grace a ce procede
EP1789531A1 (fr) * 2004-08-16 2007-05-30 PL Bio Co., Ltd. Lactobacillus rhamnosus a activite de reduction de graisse corporelle et aliments les contenant
EP1789531A4 (fr) * 2004-08-16 2008-03-19 Pl Bio Co Ltd Lactobacillus rhamnosus a activite de reduction de graisse corporelle et aliments les contenant
JP2008507991A (ja) * 2004-08-16 2008-03-21 ピーエル バイオ コーポレーション リミテッド 体脂肪低下機能性ラクトバシラスラムノサスとこれを含有した食品｛ｌａｃｔｏｂａｃｉｌｌｕｓｒｈａｍｎｏｓｕｓｗｉｔｈｂｏｄｙ‐ｆａｔｒｅｄｕｃｉｎｇａｃｔｉｖｉｔｙａｎｄｆｏｏｄｓｃｏｎｔａｉｎｉｎｇｔｈｅｍ｝
JP2008511312A (ja) * 2004-09-02 2008-04-17 ピーエル バイオ カンパニー リミテッド 体脂肪低下機能性ラクトバシラスプランタルムとこれを含有した食品（ｌａｃｔｏｂａｓｉｌｌｕｓｐｌａｎｔａｒｕｍｗｉｔｈｂｏｄｙ−ｆａｔｒｅｄｕｃｉｎｇａｃｔｉｖｉｔｙａｎｄｔｈｅｆｏｏｄｓｃｏｎｔａｉｎｉｎｇｔｈｅｍ）
CN101124318B (zh) * 2004-12-28 2013-01-09 明治制果药业株式会社 赋予宿主抗病性的新菌株及细菌细胞组合物
JPWO2006070891A1 (ja) * 2004-12-28 2008-06-12 明治製菓株式会社 宿主に対して抗病性を付与する新規な菌株および菌体組成物
WO2006070891A1 (fr) * 2004-12-28 2006-07-06 Meiji Seika Kaisha, Ltd. Nouvelle souche conferant des proprietes therapeutiques a des compositions de cellules hotes et bacteriennes
US8728794B2 (en) 2004-12-28 2014-05-20 Meiji Seika Pharma Co., Ltd. Strain conferring anti-disease properties to host and bacterial cell composition
EP1974734A1 (fr) * 2007-03-28 2008-10-01 Nestec S.A. Probiotiques pour la réduction du risque d'obésité
WO2008116700A1 (fr) * 2007-03-28 2008-10-02 Nestec S.A. Probiotiques pour la réduction du risque d'obésité
AU2008231922B2 (en) * 2007-03-28 2013-08-01 Société des Produits Nestlé S.A. Probiotics for reduction of risk of obesity
WO2009043856A2 (fr) * 2007-10-01 2009-04-09 University College Cork, National University Of Ireland, Cork Modulation de la composition en acides gras tissulaires d'un hôte par des bactéries de l'intestin humain
WO2009043856A3 (fr) * 2007-10-01 2009-07-16 Univ College Cork Nat Univ Ie Modulation de la composition en acides gras tissulaires d'un hôte par des bactéries de l'intestin humain
US8497114B2 (en) 2009-09-17 2013-07-30 Morinaga Milk Industry Co., Ltd. Anti-obesity agent, anti-obesity food or beverage, glucose tolerance-ameliorating agent, and food or beverage for amelioration of glucose tolerance
CN103981117A (zh) * 2013-12-24 2014-08-13 北京伟嘉人生物技术有限公司 一株高抗逆性屎肠球菌及其培养方法和应用
CN103981117B (zh) * 2013-12-24 2018-10-26 北京大伟嘉生物技术股份有限公司 一株高抗逆性屎肠球菌及其培养方法和应用
KR101540743B1 (ko) * 2014-04-28 2015-08-03 대한민국 공액리놀레산의 생산방법 및 콩 발효식품

Also Published As

Publication number Publication date
AU2003221135A1 (en) 2003-10-27
KR100516377B1 (ko) 2005-09-26
KR20050023364A (ko) 2005-03-09
KR100514592B1 (ko) 2005-09-13
JP4108613B2 (ja) 2008-06-25
JP2005522216A (ja) 2005-07-28
KR20030081180A (ko) 2003-10-17
KR20050023363A (ko) 2005-03-09
KR100514593B1 (ko) 2005-09-13

Similar Documents

Publication Publication Date Title
KR100514592B1 (ko) 공역화 리놀렌산을 생산하는 신규한 균주, 이를 함유하는 캡슐제 및 이를 이용한 기능성 식품
KR101401530B1 (ko) 공액리놀레산을 생산하는 비피도박테리움 롱검 균주 및 그 용도
Beheshtipour et al. Supplementation of Spirulina platensis and Chlorella vulgaris algae into probiotic fermented milks
EP3319620B1 (fr) Lactobacillus paracasei pour la production d'acide linoléique conjugué, préparations nutritionnelles et pharmaceutiques le contenant, et utilisations de ce derniers
KR20110081202A (ko) 높은 옥살산 분해능을 갖는 유산균
US10688138B2 (en) Conjugated linoleic acid-producing strains of probiotic bacteria and use thereof for the preparation of a food, dietetic or pharmaceutical composition
KR101731992B1 (ko) 면역 활성 및 안정성이 향상된 유산균을 배양하는 방법
JPWO2018003900A1 (ja) 栄養状態改善に使用するための組成物
KR101446309B1 (ko) 공액리놀레산을 생산하는 비피도박테리움 애니말리스 균주 및 그 용도
JP5603036B2 (ja) プロバイオティクス増殖促進剤
KR101655854B1 (ko) 공액리놀레산을 생산하는 락토바실러스 카제이 cbg-c16 균주 및 이의 용도
KR101191893B1 (ko) 초유로부터 분리한 공액리놀레산 생산 균주 및 그 용도
TWI734333B (zh) 減少體脂肪之益生菌株及其組合物與用途
AU2017287987A1 (en) Cartilage regeneration facilitating composition
KR101655851B1 (ko) 공액리놀레산을 생산하는 락토바실러스 애시도필러스 cbg-c13 균주 및 이의 용도
KR101655855B1 (ko) 공액리놀레산을 생산하는 락토코커스 락티스 cbg-c18 균주 및 이의 용도
KR101655853B1 (ko) 공액리놀레산을 생산하는 락토바실러스 루테리 cbg-c15 균주 및 이의 용도
KR101655852B1 (ko) 공액리놀레산을 생산하는 락토바실러스 람노서스 cbg-c14 균주 및 이의 용도
KR101664306B1 (ko) 공액리놀레산을 생산하는 락토바실러스 퍼멘텀 cbg-c17 균주 및 이의 용도
KR101655848B1 (ko) 공액리놀레산을 생산하는 비피도박테리움 비피덤 cbg-c12 균주 및 이의 용도
KR101655856B1 (ko) 공액리놀레산을 생산하는 스트렙토코커스 서모필러스 cbg-c19 균주 및 이의 용도
KR101727274B1 (ko) 젓갈로부터 분리된 공액리놀레산 생산 균주 및 이의 용도
KR101664310B1 (ko) 공액리놀레산을 생산하는 락토바실러스 가세리 cbg-c20 균주 및 이의 용도
KR101823634B1 (ko) 공액리놀레산을 생산하는 락토바실러스 헬베티커스 cbg-c23 균주 및 이의 용도
KR20030002688A (ko) 신규 미생물 비피도박테리움 브레베 ｌｍｃ7 비피더스균주, 동 균주를 이용한 공액이중결합 지방산의 생산방법및 동 균주를 이용한 발효유의 제조방법

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2003584288

Country of ref document: JP

122 Ep: pct application non-entry in european phase