WO2003078577A8 - Clonage des genes du cytochrome p450 a partir de nicotiana - Google Patents

Clonage des genes du cytochrome p450 a partir de nicotiana

Info

Publication number
WO2003078577A8
WO2003078577A8 PCT/US2003/007430 US0307430W WO03078577A8 WO 2003078577 A8 WO2003078577 A8 WO 2003078577A8 US 0307430 W US0307430 W US 0307430W WO 03078577 A8 WO03078577 A8 WO 03078577A8
Authority
WO
WIPO (PCT)
Prior art keywords
nicotiana
cytochrome
cloning
genes
Prior art date
Application number
PCT/US2003/007430
Other languages
English (en)
Other versions
WO2003078577A9 (fr
WO2003078577A2 (fr
Inventor
Dongmei Xu
Original Assignee
Us Smokeless Tobacco Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Smokeless Tobacco Co filed Critical Us Smokeless Tobacco Co
Priority to JP2003576572A priority Critical patent/JP2005531297A/ja
Priority to MXPA04008785A priority patent/MXPA04008785A/es
Priority to AU2003220165A priority patent/AU2003220165A1/en
Priority to EP03716460A priority patent/EP1572911A4/fr
Priority to APAP/P/2004/003122A priority patent/AP2004003122A0/en
Priority to CA002477452A priority patent/CA2477452A1/fr
Priority to BRPI0308351-9A priority patent/BR0308351A/pt
Priority to KR10-2004-7014250A priority patent/KR20040091124A/ko
Publication of WO2003078577A2 publication Critical patent/WO2003078577A2/fr
Publication of WO2003078577A9 publication Critical patent/WO2003078577A9/fr
Publication of WO2003078577A8 publication Critical patent/WO2003078577A8/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
PCT/US2003/007430 2002-03-12 2003-03-12 Clonage des genes du cytochrome p450 a partir de nicotiana WO2003078577A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
JP2003576572A JP2005531297A (ja) 2002-03-12 2003-03-12 タバコ由来シトクロムｐ４５０遺伝子のクローニング
MXPA04008785A MXPA04008785A (es) 2002-03-12 2003-03-12 Clonacion de genes p450 de citocromos de nicotiana.
AU2003220165A AU2003220165A1 (en) 2002-03-12 2003-03-12 Cloning of cytochrome p450 genes from nicotiana
EP03716460A EP1572911A4 (fr) 2002-03-12 2003-03-12 Clonage des genes du cytochrome p450 a partir de nicotiana
APAP/P/2004/003122A AP2004003122A0 (en) 2002-03-12 2003-03-12 Cloning of cytochrome P450 genes from nicotiana
CA002477452A CA2477452A1 (fr) 2002-03-12 2003-03-12 Clonage des genes du cytochrome p450 a partir de nicotiana
BRPI0308351-9A BR0308351A (pt) 2002-03-12 2003-03-12 molécula de ácido nucleico isolada, planta transgênica, e, métodos para produzir uma planta transgênica e para selecionar uma planta contendo uma molécula de ácido nucleico
KR10-2004-7014250A KR20040091124A (ko) 2002-03-12 2003-03-12 니코티아나 사이토크롬 ｐ450 유전자의 클로닝

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36368402P 2002-03-12 2002-03-12
US60/363,684 2002-03-12

Publications (3)

Publication Number Publication Date
WO2003078577A2 WO2003078577A2 (fr) 2003-09-25
WO2003078577A9 WO2003078577A9 (fr) 2004-01-22
WO2003078577A8 true WO2003078577A8 (fr) 2008-01-10

Family

ID=28041798

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/007430 WO2003078577A2 (fr) 2002-03-12 2003-03-12 Clonage des genes du cytochrome p450 a partir de nicotiana

Country Status (10)

Country Link
EP (1) EP1572911A4 (fr)
JP (2) JP2005531297A (fr)
KR (1) KR20040091124A (fr)
CN (1) CN101166824A (fr)
AP (1) AP2004003122A0 (fr)
AU (1) AU2003220165A1 (fr)
BR (1) BR0308351A (fr)
CA (1) CA2477452A1 (fr)
MX (1) MXPA04008785A (fr)
WO (1) WO2003078577A2 (fr)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7855318B2 (en) 2001-11-13 2010-12-21 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from Nicotiana
US7700834B2 (en) 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US7700851B2 (en) 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
JP5422099B2 (ja) * 2003-10-16 2014-02-19 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー タバコ属（Ｎｉｃｏｔｉａｎａ）からのチトクロムｐ４５０遺伝子のクローニング
EP2330191A1 (fr) 2003-10-16 2011-06-08 U.S. Smokeless Tobacco Company Clonage de gènes de cytochrome P450 à partir de Nicotiana
PL1694832T3 (pl) * 2003-10-16 2012-09-28 U S Smokeless Tobacco Company Llc Zastosowanie genu cytochromu p450 z Nicotiana
US8586837B2 (en) 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
ATE525463T1 (de) * 2004-04-29 2011-10-15 Us Smokeless Tobacco Co Genomisches tabaknikotindemethylaseklon und verwendung
CN101384710B (zh) * 2004-04-29 2012-05-02 美国无烟烟草有限责任公司 烟草烟碱脱甲基酶基因组克隆及其应用
JP5106103B2 (ja) * 2004-04-29 2012-12-26 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー タバコニコチンデメチラーゼゲノムクローンおよびその用途
JP4753937B2 (ja) * 2004-04-29 2011-08-24 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー Ｎｉｃｏｔｉａｎａ核酸分子およびその用途
AU2005243214B2 (en) * 2004-04-29 2011-07-07 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
AU2011232795B2 (en) * 2004-04-29 2015-05-28 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
CN103348912B (zh) * 2004-04-29 2016-07-13 美国无烟烟草有限责任公司 烟草属核酸分子及其应用
WO2006091194A1 (fr) 2005-02-23 2006-08-31 North Carolina State University Alteration de la teneur en alcaloide de tabac par modification des genes cytochrome p450 specifiques
ATE549399T1 (de) * 2006-12-13 2012-03-15 Dsm Sinochem Pharm Nl Bv Verfahren zur herstellung von pravastatin
US9370160B2 (en) 2006-12-15 2016-06-21 Altria Client Services Llc Tobacco inbred plants ALBEX1F and ALBEX1MS
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US8124851B2 (en) 2007-11-12 2012-02-28 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
CA2786813C (fr) 2010-01-15 2023-03-28 North Carolina State University Compositions et procedes pour reduire au minimum la synthese de nornicotine dans le tabac
US9096864B2 (en) 2011-02-28 2015-08-04 North Carolina State University Tobacco inbred plants NCBEX1F, NCBEX1MS, and NC EX90
US9603335B2 (en) 2013-01-11 2017-03-28 North Carolina State University Tobacco inbred plants K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, CMS NCTG-61 SRC and hybrid NC196 SRC
WO2014137802A1 (fr) 2013-03-05 2014-09-12 North Carolina State University Plantes autogames et hybrides de tabac et leurs utilisations
US9596823B2 (en) 2014-03-03 2017-03-21 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
EP3113604A1 (fr) 2014-03-03 2017-01-11 North Carolina State University Plantes hybrides et autogames de tabac et produits de tabac fabriqués à partir de celles-ci
EP3113603A1 (fr) 2014-03-03 2017-01-11 North Carolina State University Plantes de tabac autogames et hybrides et produits de tabac fabriqués à partir de celles-ci
KR102201082B1 (ko) 2018-10-30 2021-01-08 인하대학교 산학협력단 산소환원반응용 니켈-코발트 산화물의 산소결핍의 유도 방법 및 그 방법에 의한 니켈-코발트 산화물

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002244262B2 (en) * 2001-03-09 2007-07-12 University Of Kentucky Research Foundation Cytochrome P450s and uses thereof
MXPA05004016A (es) * 2002-10-16 2006-01-27 Us Smokeless Tobacco Co Clonacion de genes de citocromo p450 de nicotiana.

Also Published As

Publication number Publication date
JP2010131027A (ja) 2010-06-17
EP1572911A4 (fr) 2008-06-25
MXPA04008785A (es) 2005-09-30
AP2004003122A0 (en) 2004-09-30
BR0308351A (pt) 2007-04-17
CN101166824A (zh) 2008-04-23
EP1572911A2 (fr) 2005-09-14
KR20040091124A (ko) 2004-10-27
AU2003220165A8 (en) 2008-03-06
CA2477452A1 (fr) 2003-09-25
JP2005531297A (ja) 2005-10-20
WO2003078577A9 (fr) 2004-01-22
WO2003078577A2 (fr) 2003-09-25
AU2003220165A1 (en) 2003-09-29

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