WO2003070957A3 - Production de polypeptides de plantes - Google Patents

Production de polypeptides de plantes Download PDF

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Publication number
WO2003070957A3
WO2003070957A3 PCT/DK2003/000108 DK0300108W WO03070957A3 WO 2003070957 A3 WO2003070957 A3 WO 2003070957A3 DK 0300108 W DK0300108 W DK 0300108W WO 03070957 A3 WO03070957 A3 WO 03070957A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptide production
plant polypeptide
production
plant
interest
Prior art date
Application number
PCT/DK2003/000108
Other languages
English (en)
Other versions
WO2003070957A2 (fr
Inventor
Rikako Taira
Noriko Tsutsumi
Yuri Terui
Shinobu Takagi
Original Assignee
Novozymes As
Rikako Taira
Noriko Tsutsumi
Yuri Terui
Shinobu Takagi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As, Rikako Taira, Noriko Tsutsumi, Yuri Terui, Shinobu Takagi filed Critical Novozymes As
Priority to AU2003206684A priority Critical patent/AU2003206684A1/en
Publication of WO2003070957A2 publication Critical patent/WO2003070957A2/fr
Publication of WO2003070957A3 publication Critical patent/WO2003070957A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants

Abstract

L'invention concerne un procédé permettant d'augmenter l'expression et la production de polypeptides de plantes spécifiques dans des cellules hôtes de champignons filamenteux.
PCT/DK2003/000108 2002-02-20 2003-02-19 Production de polypeptides de plantes WO2003070957A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003206684A AU2003206684A1 (en) 2002-02-20 2003-02-19 Plant polypeptide production

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200200263 2002-02-20
DKPA200200263 2002-02-20
DKPA200200871 2002-06-07
DKPA200200871 2002-06-07

Publications (2)

Publication Number Publication Date
WO2003070957A2 WO2003070957A2 (fr) 2003-08-28
WO2003070957A3 true WO2003070957A3 (fr) 2003-12-24

Family

ID=27758691

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2003/000108 WO2003070957A2 (fr) 2002-02-20 2003-02-19 Production de polypeptides de plantes

Country Status (2)

Country Link
AU (1) AU2003206684A1 (fr)
WO (1) WO2003070957A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8812247B2 (en) 2006-06-29 2014-08-19 Dsm Ip Assets B.V. Method for achieving improved polypeptide expression

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004050695A2 (fr) * 2002-12-05 2004-06-17 Novozymes A/S Expression de polypeptides dans des champignons filamenteux
EP1685256A1 (fr) * 2003-10-31 2006-08-02 Novozymes A/S Procede de floculation d'un bouillon de fermentation comprenant un champignon
US7785872B2 (en) * 2004-12-08 2010-08-31 Simpson Biotech Co., Ltd. Nucleic acids for enhancing gene expression and use thereof
CA2595056A1 (fr) * 2005-01-24 2006-07-27 Dsm Ip Assets B.V. Procede de fabrication d'un compose d'interet dans une cellule fongique filamenteuse
WO2009005564A2 (fr) * 2007-06-29 2009-01-08 The Regents Of The University Of California Séquences nucléotidiques codant pour l'enzyme dégradant la cellulose et l'hémicellulose et ayant une cinétique traductionnelle raffinée, et procédé de production correspondant
CN103443268A (zh) * 2011-01-20 2013-12-11 诺维信公司 植物过氧化物酶在丝状真菌中的表达
CN104245937B (zh) 2012-04-17 2021-09-21 弗·哈夫曼-拉罗切有限公司 使用修饰的核酸表达多肽的方法
CN102776215B (zh) * 2012-06-13 2014-08-27 中国农业科学院生物技术研究所 优化的乳糖酶基因及其分泌表达方法和应用
US10724040B2 (en) 2015-07-15 2020-07-28 The Penn State Research Foundation mRNA sequences to control co-translational folding of proteins
CN107353327A (zh) 2017-03-30 2017-11-17 南京百斯杰生物工程有限公司 植酸酶在黑曲霉中表达
AU2019380861A1 (en) * 2018-11-13 2021-06-03 Kabushiki Kaisha Yakult Honsha Method for producing secreted beta-galactosidase
BR112021025806A2 (pt) 2019-06-28 2022-02-08 Hoffmann La Roche Métodos para produzir um anticorpo da subclasse igg1 humana cultivando uma célula cho e uso da remoção de sítios de splice de doador não pareados em uma parte de uma sequência de ácido nucleico de humano ou hamster

Citations (6)

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Publication number Priority date Publication date Assignee Title
WO1987004464A1 (fr) * 1986-01-17 1987-07-30 Zymogenetics, Inc. Expression de genes eucaryotiques superieurs dans l'aspergillus
WO1993011249A1 (fr) * 1991-12-04 1993-06-10 Novo Nordisk A/S PROCEDE DE CLONAGE DE PROTEINES DANS LA LEVURE ET CELLULASE PROVENANT DE $i(HUMICOLA INSOLENS)
EP0684312A2 (fr) * 1994-04-21 1995-11-29 Urquima S.A. Préparation d'une protéine naturelle édulcorante
JPH09121870A (ja) * 1994-12-29 1997-05-13 Ajinomoto Co Inc 新規チオールプロテアーゼをコードするｄｎａおよびこれを用いた該チオールプロテアーゼの製造方法
WO1999051756A2 (fr) * 1998-04-02 1999-10-14 Urquima, S.A. Promoteur et produits de synthese destines a l'expression de proteines de recombinaison dans des champignons filamenteux
WO2000039322A1 (fr) * 1998-12-23 2000-07-06 Novozymes A/S Procedes permettant de produire des polypeptides dans des cellules mutantes d'aspergillus

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987004464A1 (fr) * 1986-01-17 1987-07-30 Zymogenetics, Inc. Expression de genes eucaryotiques superieurs dans l'aspergillus
WO1993011249A1 (fr) * 1991-12-04 1993-06-10 Novo Nordisk A/S PROCEDE DE CLONAGE DE PROTEINES DANS LA LEVURE ET CELLULASE PROVENANT DE $i(HUMICOLA INSOLENS)
EP0684312A2 (fr) * 1994-04-21 1995-11-29 Urquima S.A. Préparation d'une protéine naturelle édulcorante
JPH09121870A (ja) * 1994-12-29 1997-05-13 Ajinomoto Co Inc 新規チオールプロテアーゼをコードするｄｎａおよびこれを用いた該チオールプロテアーゼの製造方法
WO1999051756A2 (fr) * 1998-04-02 1999-10-14 Urquima, S.A. Promoteur et produits de synthese destines a l'expression de proteines de recombinaison dans des champignons filamenteux
WO2000039322A1 (fr) * 1998-12-23 2000-07-06 Novozymes A/S Procedes permettant de produire des polypeptides dans des cellules mutantes d'aspergillus

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
FAUS I ET AL: "Secretion of the sweet-tasting protein thaumatin by recombinant strains of Aspergillus niger var. awamori.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 49, no. 4, April 1998 (1998-04-01), pages 393 - 398, XP002205862, ISSN: 0175-7598 *
FAUS IGNACIO ET AL: "Expression of a synthetic gene encoding the sweet-tasting protein thaumatin in Escherichia coli.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 229, no. 1, 1996, pages 121 - 127, XP002205863, ISSN: 0006-291X *
HERZOG R W ET AL: "Expression of a synthetic protein-based polymer (elastomer) gene in Aspergillus nidulans.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 47, no. 4, 1997, pages 368 - 372, XP002205867, ISSN: 0175-7598 *
JUGE N ET AL: "Secretion, purification, and characterisation of barley alpha-amylase produced by heterologous gene expression in Aspergillus niger.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 49, no. 4, April 1998 (1998-04-01), pages 385 - 392, XP002205866, ISSN: 0175-7598 *
MORALEJO F J ET AL: "Overexpression and lack of degradation of thaumatin in an aspergillopepsin A-defective mutant of Aspergillus awamori containing an insertion in the pepA gene.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 54, no. 6, December 2000 (2000-12-01), pages 772 - 777, XP002205865, ISSN: 0175-7598 *
MORALEJO FRANCISCO-JOSE ET AL: "Thaumatin production in Aspergillus awamori by use of expression cassettes with strong fungal promoters and high gene dosage.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 65, no. 3, March 1999 (1999-03-01), pages 1168 - 1174, XP002205864, ISSN: 0099-2240 *
PATENT ABSTRACTS OF JAPAN vol. 1997, no. 09 30 September 1997 (1997-09-30) *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8812247B2 (en) 2006-06-29 2014-08-19 Dsm Ip Assets B.V. Method for achieving improved polypeptide expression

Also Published As

Publication number Publication date
WO2003070957A2 (fr) 2003-08-28
AU2003206684A8 (en) 2003-09-09
AU2003206684A1 (en) 2003-09-09

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