WO2003066896A3 - Verfahren zum nachweis von mutationen - Google Patents

Verfahren zum nachweis von mutationen Download PDF

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Publication number
WO2003066896A3
WO2003066896A3 PCT/EP2003/001258 EP0301258W WO03066896A3 WO 2003066896 A3 WO2003066896 A3 WO 2003066896A3 EP 0301258 W EP0301258 W EP 0301258W WO 03066896 A3 WO03066896 A3 WO 03066896A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligos
linkage
complex
mutations
analysis
Prior art date
Application number
PCT/EP2003/001258
Other languages
English (en)
French (fr)
Other versions
WO2003066896A2 (de
Inventor
Christian Albrecht
Filipp Oesterhelt
Boris Steipe
Hermann Gaub
Hauke Clausen-Schaumann
Michael Cieplik
Original Assignee
Nanotype Gmbh
Christian Albrecht
Filipp Oesterhelt
Boris Steipe
Hermann Gaub
Hauke Clausen-Schaumann
Michael Cieplik
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE2002105419 external-priority patent/DE10205419A1/de
Priority claimed from DE2002107918 external-priority patent/DE10207918A1/de
Application filed by Nanotype Gmbh, Christian Albrecht, Filipp Oesterhelt, Boris Steipe, Hermann Gaub, Hauke Clausen-Schaumann, Michael Cieplik filed Critical Nanotype Gmbh
Priority to AU2003244501A priority Critical patent/AU2003244501A1/en
Priority to EP03737328A priority patent/EP1565569A2/de
Publication of WO2003066896A2 publication Critical patent/WO2003066896A2/de
Publication of WO2003066896A3 publication Critical patent/WO2003066896A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Es wird ein Verfahren zum Nachweis von Mutationen in einer Nucleinsäure beschrieben, das umfasst, dass a) eine Mehrzahl zu analysierender Nucleinsäure-Stränge mit mindestens zwei Arten von Oligonucleotiden, Rezeptoroligos und Sondenoligos, in Kontakt gebracht wird unter solchen Bedingungen, dass die Oligos mit der zu analysierenden DNA hybridisieren unter Bildung eines Probenkomplexes und eines Referenzkomplexes, so dass eine beidseitig immobilisierte oder immobilisierbare Verkettung von Probekomplex und Referenzkomplex vorliegt, b) anschliessend gegebenenfalls die Verkettung immobilisiert, c) an die Verkettung eine Zugkraft angelegt wird, bis sich einer der Komplexe trennt und d) bestimmt wird, welcher der Komplexe getrennt wurde.
PCT/EP2003/001258 2002-02-09 2003-02-07 Verfahren zum nachweis von mutationen WO2003066896A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003244501A AU2003244501A1 (en) 2002-02-09 2003-02-07 Method for the detection of mutations
EP03737328A EP1565569A2 (de) 2002-02-09 2003-02-07 Verfahren zum nachweis von mutationen

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10205419.3 2002-02-09
DE2002105419 DE10205419A1 (de) 2002-02-09 2002-02-09 Verfahren zum Nachweis von Mutationen
DE2002107918 DE10207918A1 (de) 2002-02-23 2002-02-23 Verfahren zum Nachweis von Mutationen
DE10207918.8 2002-02-23

Publications (2)

Publication Number Publication Date
WO2003066896A2 WO2003066896A2 (de) 2003-08-14
WO2003066896A3 true WO2003066896A3 (de) 2004-03-25

Family

ID=27735663

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/001258 WO2003066896A2 (de) 2002-02-09 2003-02-07 Verfahren zum nachweis von mutationen

Country Status (3)

Country Link
EP (1) EP1565569A2 (de)
AU (1) AU2003244501A1 (de)
WO (1) WO2003066896A2 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10126798A1 (de) 2001-06-01 2002-12-19 Nanotype Gmbh Verfahren zur Bestimmung einer Probe
KR100580644B1 (ko) 2004-02-16 2006-05-16 삼성전자주식회사 생물분자를 고체 기판상에 비공유적으로 고정화 하는 방법및 그에 의하여 제조되는 마이크로어레이
EP2390350A1 (de) 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Verfahren zur DNA-Sequenzierung durch Polymerisation
EP2390351A1 (de) 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Verfahren zur DNA-Sequenzierung durch Hybridisierung
FR2993282B1 (fr) 2012-07-13 2017-11-10 Expanscience Lab Procede d'identification de marqueurs moleculaires de la peau d'enfant
FR3001889B1 (fr) 2013-02-11 2021-02-12 Expanscience Lab Utilisation d'une composition comprenant un perseose d'avocat dans la protection des cellules souches epidermiques .
FR3011008B1 (fr) 2013-09-24 2017-12-29 Expanscience Lab Procedes d'evaluation des effets deleteres des uv sur la peau d'enfant
EP2886663A1 (de) 2013-12-19 2015-06-24 Centre National de la Recherche Scientifique (CNRS) Nanoporensequenzierung mit replikativen Polymerasen und Helicasen
FR3016373B1 (fr) 2014-01-10 2018-01-19 Laboratoires Expanscience Modele de peau de mammelon reconstitue
FR3019186B1 (fr) 2014-03-31 2019-06-07 Laboratoires Expanscience Procedes d'evaluation des effets deleteres de l'urine sur la peau d'enfant
FR3045669B1 (fr) 2015-12-16 2019-04-05 Laboratoires Expanscience Procedes d'evaluation des effets de la deshydratation sur la peau d'enfant
FR3053053B1 (fr) 2016-06-23 2018-07-13 Laboratoires Expanscience Modeles de la dermatite atopique juvenile
FR3059550B1 (fr) 2016-12-01 2020-01-03 Universite De Rouen Normandie Traitement des troubles causes par l'alcoolisation foetale (tcaf)
FR3068045B1 (fr) 2017-06-22 2021-06-04 Expanscience Lab Modeles de peau sensible reconstituee
FR3081707A1 (fr) 2018-05-30 2019-12-06 Universite De Rouen Normandie Traitement des troubles neurologiques causes par l'alcoolisation foetale
WO2020016459A1 (en) 2018-07-20 2020-01-23 Pierre Fabre Medicament Receptor for vista
FR3091351B1 (fr) 2018-12-27 2021-05-21 Univ Rouen Centre Hospitalier Biomarqueur de la maladie de fabry
FR3098405B1 (fr) 2019-07-12 2021-06-25 Expanscience Lab Composition comprenant des polyphénols de graines de passiflore, des peptides d’avocat et un extrait d’hamamélis et utilisation pour traiter et/ou prévenir les vergetures
EP3922641A1 (de) 2020-06-09 2021-12-15 Institut Gustave-Roussy Verfahren zum nachweis und zur behandlung von covid-patienten, die intensivpflege benötigen
FR3133198A1 (fr) 2022-03-04 2023-09-08 Pierre Fabre Dermo-Cosmetique Modele de peau reconstituee

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6342353B1 (en) * 1997-02-21 2002-01-29 Centre National De La Recherche Scientifique (Cnrs) Method for characterizing nucleic acid duplex
WO2002014862A2 (de) * 2000-08-11 2002-02-21 Nanotype Gmbh Verfahren und vorrichtung zur charakterisierung und/oder zum nachweis eines bindungskomplexes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6342353B1 (en) * 1997-02-21 2002-01-29 Centre National De La Recherche Scientifique (Cnrs) Method for characterizing nucleic acid duplex
WO2002014862A2 (de) * 2000-08-11 2002-02-21 Nanotype Gmbh Verfahren und vorrichtung zur charakterisierung und/oder zum nachweis eines bindungskomplexes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ALBRECHT CHRISTIAN ET AL: "DNA: a programmable force sensor.", SCIENCE. UNITED STATES 18 JUL 2003, vol. 301, no. 5631, 18 July 2003 (2003-07-18), pages 367 - 370, XP002256755, ISSN: 1095-9203 *
NOY A ET AL: "STRETCHING AND BREAKING DUPLEX DNA BY CHEMICAL FORCE MICROSCOPY", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 4, no. 7, 1 July 1997 (1997-07-01), pages 519 - 527, XP002071033, ISSN: 1074-5521 *
STRUNZ TORSTEN ET AL: "Dynamic force spectroscopy of single DNA molecules", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 96, no. 20, 28 September 1999 (1999-09-28), Sept. 28, 1999, pages 11277 - 11282, XP002256754, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
AU2003244501A1 (en) 2003-09-02
EP1565569A2 (de) 2005-08-24
WO2003066896A2 (de) 2003-08-14

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