WO2003046161A3 - Verfahren zur ex vivo-expansion und -differenzierung von multipotenten stammzellen - Google Patents

Verfahren zur ex vivo-expansion und -differenzierung von multipotenten stammzellen Download PDF

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Publication number
WO2003046161A3
WO2003046161A3 PCT/EP2002/013142 EP0213142W WO03046161A3 WO 2003046161 A3 WO2003046161 A3 WO 2003046161A3 EP 0213142 W EP0213142 W EP 0213142W WO 03046161 A3 WO03046161 A3 WO 03046161A3
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stem cells
vivo
expansion
multipotent stem
differentiation
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PCT/EP2002/013142
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English (en)
French (fr)
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WO2003046161A2 (de
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Dieter Kurt Hossfeld
Walter Fiedler
Ursula Gehling
Sonja Loges
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Universitaetsklinikum Hamburg
Dieter Kurt Hossfeld
Walter Fiedler
Ursula Gehling
Sonja Loges
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Application filed by Universitaetsklinikum Hamburg, Dieter Kurt Hossfeld, Walter Fiedler, Ursula Gehling, Sonja Loges filed Critical Universitaetsklinikum Hamburg
Priority to EP02787775A priority Critical patent/EP1453951A2/de
Priority to AU2002352100A priority patent/AU2002352100A1/en
Priority to US10/497,101 priority patent/US20060051330A1/en
Publication of WO2003046161A2 publication Critical patent/WO2003046161A2/de
Publication of WO2003046161A3 publication Critical patent/WO2003046161A3/de

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Abstract

Die Erfindung betrifft ein Verfahren zur Expansion multipotenter Stammzellen ex vivo. Ferner betrifft die Erfindung ein zweistufiges Verfahren zur Expansion und Differenzierung von multipotenten Stammzellen ex vivo, bei dem die Stammzellen auf der ersten Stufe, d.h. während der Expansionsphase, gentransfiziert werden können. In der Phase erfolgt die Differenzierung der multipotenten Stammzellen wahlweise in Zellen der hämatopoetischen, endothelialen oder mesenchymalen Zellreihe. Auf diese Weise gewonnene Stamm- und Progenitorzellen sowie reife Zellen der hämatopoetischen, endothelialen und mesenchymalen Zellreihe lassen sich unter anderem für die Prophylaxe, Diagnostik und Therapie humaner Erkrankungen sowie zum Tissue Engineering einsetzen.
PCT/EP2002/013142 2001-11-30 2002-11-22 Verfahren zur ex vivo-expansion und -differenzierung von multipotenten stammzellen WO2003046161A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02787775A EP1453951A2 (de) 2001-11-30 2002-11-22 Verfahren zur ex vivo-expansion und -differenzierung von multipotenten stammzellen
AU2002352100A AU2002352100A1 (en) 2001-11-30 2002-11-22 Method for carrying out the ex vivo expansion and ex vivo differentiation of multipotent stem cells
US10/497,101 US20060051330A1 (en) 2001-11-30 2002-11-22 Method for carrying out the ex vivo expansion and ex vivo differentiation of multipotent stem cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10158680A DE10158680B4 (de) 2001-11-30 2001-11-30 Verfahren zur ex vivo-Expansion und -Differenzierung von multipotenten Stammzellen
DE10158680.9 2001-11-30

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WO2003046161A2 WO2003046161A2 (de) 2003-06-05
WO2003046161A3 true WO2003046161A3 (de) 2004-02-12

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US (1) US20060051330A1 (de)
EP (1) EP1453951A2 (de)
AU (1) AU2002352100A1 (de)
DE (1) DE10158680B4 (de)
WO (1) WO2003046161A2 (de)

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US20050265980A1 (en) * 2004-05-14 2005-12-01 Becton, Dickinson And Company Cell culture environments for the serum-free expansion of mesenchymal stem cells
WO2006086639A1 (en) * 2005-02-10 2006-08-17 Regents Of The University Of Minnesota Vascular/lymphatic endothelial cells
EP1924606A4 (de) * 2005-08-25 2010-01-13 Repair Technologies Inc Vorrichtungen, zusammensetzungen und verfahren zum schutz und zur reparatur von zellen und gewebe
WO2007117472A2 (en) * 2006-04-07 2007-10-18 Los Angeles Biomedical Research Institute At Harbor-Ucla Medical Center Adult bone marrow cell transplantation to testes creation of transdifferentiated testes germ cells, leydig cells and sertoli cells
BE1018748A3 (fr) * 2008-05-07 2011-08-02 Bone Therapeutics Sa Nouvelles cellules souches mesenchymateuses et cellules osteogeniques .
WO2012048275A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
JP6633522B2 (ja) 2013-11-16 2020-01-22 テルモ ビーシーティー、インコーポレーテッド バイオリアクターにおける細胞増殖
WO2015148704A1 (en) 2014-03-25 2015-10-01 Terumo Bct, Inc. Passive replacement of media
JP6830059B2 (ja) 2014-09-26 2021-02-17 テルモ ビーシーティー、インコーポレーテッド スケジュール化された細胞フィーディング
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
WO2017205667A1 (en) 2016-05-25 2017-11-30 Terumo Bct, Inc. Cell expansion
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11629332B2 (en) 2017-03-31 2023-04-18 Terumo Bct, Inc. Cell expansion
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
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DE10158680A1 (de) 2003-06-12
US20060051330A1 (en) 2006-03-09
AU2002352100A8 (en) 2003-06-10
DE10158680B4 (de) 2004-04-08
EP1453951A2 (de) 2004-09-08
WO2003046161A2 (de) 2003-06-05

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