WO2003025182A1 - Dna for overexpression of growth hormone and utilization thereof - Google Patents

Dna for overexpression of growth hormone and utilization thereof Download PDF

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Publication number
WO2003025182A1
WO2003025182A1 PCT/JP2002/009155 JP0209155W WO03025182A1 WO 2003025182 A1 WO2003025182 A1 WO 2003025182A1 JP 0209155 W JP0209155 W JP 0209155W WO 03025182 A1 WO03025182 A1 WO 03025182A1
Authority
WO
WIPO (PCT)
Prior art keywords
dna
growth hormone
amino acid
linker
expression
Prior art date
Application number
PCT/JP2002/009155
Other languages
French (fr)
Japanese (ja)
Inventor
Seiji Sato
Masaaki Kondo
Toshiyuki Kudo
Kosuke Endo
Shigeaki Watanabe
Yoshihiro Waki
Masaya Yamanaka
Original Assignee
Itoham Foods Inc.
Udaka, Shigezo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Itoham Foods Inc., Udaka, Shigezo filed Critical Itoham Foods Inc.
Publication of WO2003025182A1 publication Critical patent/WO2003025182A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A DNA encoding a fused protein wherein a leader peptide (Leader) consisting of one or more amino acid residues from the N-terminus of a cell wall protein (CWP) of a bacillus, an amino acid sequence (Cleavage) to be used in enzymatic cleavage and an amino acid sequence (GH) of growth hormone, its functional fragment, variant or analog are appropriately ligated to nH consisting of a plural number of His residues and a linker (Linker). Another DNA further containing a DNA sequence enabling the expression of the above DNA, a vector containing this DNA allowing the expression, host cells containing this vector and a process for producing a recombinant growth hormone by using these host cells are further disclosed.
PCT/JP2002/009155 2001-09-13 2002-09-09 Dna for overexpression of growth hormone and utilization thereof WO2003025182A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001278534A JP2003079379A (en) 2001-09-13 2001-09-13 Dna for achieving high expression of growth hormone and use thereof
JP2001-278534 2001-09-13

Publications (1)

Publication Number Publication Date
WO2003025182A1 true WO2003025182A1 (en) 2003-03-27

Family

ID=19102886

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/009155 WO2003025182A1 (en) 2001-09-13 2002-09-09 Dna for overexpression of growth hormone and utilization thereof

Country Status (3)

Country Link
JP (1) JP2003079379A (en)
CN (1) CN1547610A (en)
WO (1) WO2003025182A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2251425B1 (en) 2004-07-06 2016-04-20 Kaneka Corporation Process for producing protein A-like protein with use of Brevibacillus genus bacterium
CN102389575B (en) * 2011-11-18 2013-01-23 武汉凯肽来生物科技有限公司 Genetic engineering oral DNA vaccine and preparation method and application
CN107142235A (en) * 2017-06-20 2017-09-08 江西嘉博生物工程有限公司 A kind of restructuring Brevibacillus brevis for expressing pig growth hormone gene and construction method and application
CN109957028B (en) * 2017-12-22 2023-04-07 丰益(上海)生物技术研发中心有限公司 Methods for increasing production of mature peptides of secreted proteins

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0534568A2 (en) * 1991-09-27 1993-03-31 ENIRICERCHE S.p.A. Human growth hormone analogues and their use
JPH11341991A (en) * 1998-03-31 1999-12-14 Itoham Foods Inc Dna coding for new fused protein and production of useful polypeptide through expression of the same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0534568A2 (en) * 1991-09-27 1993-03-31 ENIRICERCHE S.p.A. Human growth hormone analogues and their use
JPH11341991A (en) * 1998-03-31 1999-12-14 Itoham Foods Inc Dna coding for new fused protein and production of useful polypeptide through expression of the same

Also Published As

Publication number Publication date
JP2003079379A (en) 2003-03-18
CN1547610A (en) 2004-11-17

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