WO2003018834A3 - Essai de deplacement destine a la detection d'hybridations d'oligomeres d'acide nucleique - Google Patents

Essai de deplacement destine a la detection d'hybridations d'oligomeres d'acide nucleique Download PDF

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Publication number
WO2003018834A3
WO2003018834A3 PCT/DE2002/003122 DE0203122W WO03018834A3 WO 2003018834 A3 WO2003018834 A3 WO 2003018834A3 DE 0203122 W DE0203122 W DE 0203122W WO 03018834 A3 WO03018834 A3 WO 03018834A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid oligomer
detection
hybridization events
ligands
Prior art date
Application number
PCT/DE2002/003122
Other languages
German (de)
English (en)
Other versions
WO2003018834A2 (fr
Inventor
Gerhard Hartwich
Peter Frischmann
Ute Haker
Herbert Wieder
Original Assignee
Friz Biochem Gmbh
Gerhard Hartwich
Peter Frischmann
Ute Haker
Herbert Wieder
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10141691A external-priority patent/DE10141691A1/de
Application filed by Friz Biochem Gmbh, Gerhard Hartwich, Peter Frischmann, Ute Haker, Herbert Wieder filed Critical Friz Biochem Gmbh
Priority to AT02762252T priority Critical patent/ATE513058T1/de
Priority to EP02762252A priority patent/EP1554396B1/fr
Priority to US10/487,816 priority patent/US20060228703A1/en
Publication of WO2003018834A2 publication Critical patent/WO2003018834A2/fr
Publication of WO2003018834A3 publication Critical patent/WO2003018834A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé destiné à la détection d'hybridations d'oligomères d'acide nucléique, ledit procédé comprenant les étapes suivantes: mise à disposition d'une surface modifiée, la modification correspondant à la liaison d'au moins un type d'oligomères d'acide nucléique de ligature; mise à disposition de ligands d'oligomères d'acide nucléique signal; mise à disposition d'un échantillon comprenant des ligands d'oligomères d'acide nucléique; mise en contact d'une quantité définie des ligands d'oligomères d'acide nucléique signal avec la surface modifiée, et mise en contact de l'échantillon et des ligands d'oligomères d'acide nucléique qu'il contient, avec la surface modifiée; détection des ligands d'oligomères d'acide nucléique signal; et comparaison des valeurs obtenues pour la détection des ligands d'oligomères d'acide nucléique signal avec des valeurs de référence.
PCT/DE2002/003122 2001-08-25 2002-08-26 Essai de deplacement destine a la detection d'hybridations d'oligomeres d'acide nucleique WO2003018834A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AT02762252T ATE513058T1 (de) 2001-08-25 2002-08-26 Verdrängungsassay zur detektion von nukleinsäureoligomer-hybridisierungsereignissen
EP02762252A EP1554396B1 (fr) 2001-08-25 2002-08-26 Essai de deplacement destine a la detection d'hybridations d'oligomeres d'acide nucleique
US10/487,816 US20060228703A1 (en) 2001-08-25 2002-08-26 Displacement assay for the detection of nucleic acid oligomer hybridization events

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10141691A DE10141691A1 (de) 2001-08-25 2001-08-25 Verdrängungsassay zur Detektion von Ligat-Ligand-Assoziationsereignissen
DE10141691.1 2001-08-25
DEPCT/DE02/01269 2002-04-06
PCT/DE2002/001269 WO2003019194A2 (fr) 2001-08-25 2002-04-06 Essai de deplacement pour la detection d'evenements d'association ligat-ligand

Publications (2)

Publication Number Publication Date
WO2003018834A2 WO2003018834A2 (fr) 2003-03-06
WO2003018834A3 true WO2003018834A3 (fr) 2003-09-12

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2002/003122 WO2003018834A2 (fr) 2001-08-25 2002-08-26 Essai de deplacement destine a la detection d'hybridations d'oligomeres d'acide nucleique

Country Status (1)

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WO (1) WO2003018834A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005031066A (ja) * 2003-04-28 2005-02-03 Canon Inc 固定化核酸プローブの処理方法、核酸の検出方法、核酸濃度分析方法、および核酸検出用キット
DE102007044664B4 (de) 2007-09-18 2012-01-05 Friz Biochem Gesellschaft Für Bioanalytik Mbh Verdrängungsassay zur Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen
DE102008002267B3 (de) 2008-06-06 2009-11-26 Friz Biochem Gesellschaft Für Bioanalytik Mbh Elektrisches Subtrat zum Einsatz als Träger von Biomolekülen
DE102009044795B3 (de) 2009-12-07 2011-04-07 Friz Biochem Gesellschaft Für Bioanalytik Mbh Kompetitionsassay zur Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen
DE102011056606B3 (de) 2011-12-19 2013-01-03 Friz Biochem Gesellschaft Für Bioanalytik Mbh Verfahren zur elektrochemischen Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993021530A1 (fr) * 1992-04-10 1993-10-28 Messerschmitt-Bölkow-Blohm Gmbh Procede et dispositif visant a augmenter la sensibilite et la selectivite pour les dosages immunologiques, les dosages d'interaction molecule-recepteur, adn complementaire - adn et molecule hote - molecule etrangere
WO1999051778A1 (fr) * 1998-04-08 1999-10-14 California Institute Of Technology Capteur electrochimique utilisant des fractions intercalaires a activite redox
WO1999057319A1 (fr) * 1998-05-06 1999-11-11 Clinical Micro Sensors Detection electronique d'acides nucleiques a l'aide de monocouches
WO2001007665A2 (fr) * 1999-07-26 2001-02-01 Clinical Micro Sensors, Inc. Determination de sequences d'acides nucleiques par detection electronique

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU738189B2 (en) 1996-06-07 2001-09-13 Ge Healthcare (Sv) Corp. Nucleic acid mediated electron transfer
DE19964220C2 (de) 1998-11-23 2003-07-03 Friz Biochem Gmbh Verfahren zur Herstellung einer modifizierten leitfähigen Oberfläche
SI1144685T1 (en) 1999-01-18 2003-10-31 Friz Biochem Gmbh Method for electrochemically detecting nucleic acid-oligomer hybridisation events

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993021530A1 (fr) * 1992-04-10 1993-10-28 Messerschmitt-Bölkow-Blohm Gmbh Procede et dispositif visant a augmenter la sensibilite et la selectivite pour les dosages immunologiques, les dosages d'interaction molecule-recepteur, adn complementaire - adn et molecule hote - molecule etrangere
WO1999051778A1 (fr) * 1998-04-08 1999-10-14 California Institute Of Technology Capteur electrochimique utilisant des fractions intercalaires a activite redox
WO1999057319A1 (fr) * 1998-05-06 1999-11-11 Clinical Micro Sensors Detection electronique d'acides nucleiques a l'aide de monocouches
WO2001007665A2 (fr) * 1999-07-26 2001-02-01 Clinical Micro Sensors, Inc. Determination de sequences d'acides nucleiques par detection electronique

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MARRAZZA G ET AL: "Disposable DNA electrochemical sensor for hybridization detection", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 14, 1999, pages 43 - 51, XP002113746, ISSN: 0956-5663 *
UMEK R M ET AL: "Bioelectronic detection of point mutations using discrimination of the H63D polymorphism of the Hfe gene as a model.", MOLECULAR DIAGNOSIS: A JOURNAL DEVOTED TO THE UNDERSTANDING OF HUMAN DISEASE THROUGH THE CLINICAL APPLICATION OF MOLECULAR BIOLOGY. UNITED STATES DEC 2000, vol. 5, no. 4, December 2000 (2000-12-01), pages 321 - 328, XP008018952, ISSN: 1084-8592 *
WANG J ET AL: "Nucleic-acid immobilization, recognition and detection at chronopotentiometric DNA chips", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 12, no. 7, 1997, pages 587 - 599, XP002113741, ISSN: 0956-5663 *

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Publication number Publication date
WO2003018834A2 (fr) 2003-03-06

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