WO2003012447A3 - Nachweis lebender mikroorganismen, eukaryotischer zellen oder organellen - Google Patents

Nachweis lebender mikroorganismen, eukaryotischer zellen oder organellen Download PDF

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Publication number
WO2003012447A3
WO2003012447A3 PCT/DE2002/002702 DE0202702W WO03012447A3 WO 2003012447 A3 WO2003012447 A3 WO 2003012447A3 DE 0202702 W DE0202702 W DE 0202702W WO 03012447 A3 WO03012447 A3 WO 03012447A3
Authority
WO
WIPO (PCT)
Prior art keywords
organelles
identification
eukaryotic cells
living microorganisms
microorganisms
Prior art date
Application number
PCT/DE2002/002702
Other languages
English (en)
French (fr)
Other versions
WO2003012447A2 (de
Inventor
Rainer Feldbruegge
Christiane Zaborosch
Andreas Katerkamp
Maik Brinkmann
Original Assignee
Inst Chemo Biosensorik
Rainer Feldbruegge
Christiane Zaborosch
Andreas Katerkamp
Maik Brinkmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Chemo Biosensorik, Rainer Feldbruegge, Christiane Zaborosch, Andreas Katerkamp, Maik Brinkmann filed Critical Inst Chemo Biosensorik
Priority to AU2002320995A priority Critical patent/AU2002320995A1/en
Priority to DE10293412T priority patent/DE10293412D2/de
Publication of WO2003012447A2 publication Critical patent/WO2003012447A2/de
Publication of WO2003012447A3 publication Critical patent/WO2003012447A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/002—Electrode membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/255—Salmonella (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Inorganic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Die Erfindung betrifft eine Vorrichtung und ein Verfahren für de n Nachweis lebender Mikroorganismen, eukaryotischen Zellen oder Organellen in einem Probenvolumen. Die Erfindung ist insbesondere für den Nachweis lebender Zellen der Gattung Salmonella geeignet. Mit der Erfindung soll eine Nachweismöglichkeit für lebende Mikroorganismen, eukaryotische Zellen oder Organellen geschaffen werden, bei der der Zeit- und Kostenaufwand, bei gleichzeitig guter Nachweisgenauigkeit verringert werden kann. Zur Lösung dieser Aufgabe werden magnetisierbare Partikel verwendet, an deren Oberfläche für mindestens Mikroorganismus, eukaryotische Zellen oder Organellen spezifische Bindungsmoleküle gebunden sind. Die magnetisierbaren Partikel werden in das Probenvolumen zu einer lokalen Konzentration mit Hilfe eines Permanent- oder Elektromagneten gegeben und für den Nachweis mindestens ein Sensor zur Bestimmung von sich stoffwechselabhängig veranderbaren Stoffkonzentrationen oder der Bildung von Stoffwechselprodukten in den Mikroorganismen, eukaryotischen Zellen oder Organellen verwendet.
PCT/DE2002/002702 2001-07-23 2002-07-22 Nachweis lebender mikroorganismen, eukaryotischer zellen oder organellen WO2003012447A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002320995A AU2002320995A1 (en) 2001-07-23 2002-07-22 Identification of living microorganisms, eukaryotic cells or organelles
DE10293412T DE10293412D2 (de) 2001-07-23 2002-07-22 Vorrichtung und Verfahren für den Nachweis lebender Mikroorganismen, eukaryotischer Zeller oder Organellen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10136472.5 2001-07-23
DE2001136472 DE10136472A1 (de) 2001-07-23 2001-07-23 Vorrichtung und Verfahren für den Nachweis lebender Mikroorganismen, eukaryotischer Zellen oder Organellen

Publications (2)

Publication Number Publication Date
WO2003012447A2 WO2003012447A2 (de) 2003-02-13
WO2003012447A3 true WO2003012447A3 (de) 2003-08-07

Family

ID=7693204

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2002/002702 WO2003012447A2 (de) 2001-07-23 2002-07-22 Nachweis lebender mikroorganismen, eukaryotischer zellen oder organellen

Country Status (3)

Country Link
AU (1) AU2002320995A1 (de)
DE (2) DE10136472A1 (de)
WO (1) WO2003012447A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008097155A1 (en) * 2007-02-08 2008-08-14 Ge Healthcare Bio-Sciences Ab Media and method for cell separation
WO2010151131A2 (en) * 2009-06-26 2010-12-29 Nederlandse Organisatie Voor Toegepast- Natuurwetenschappelijk Onderzoek Tno Apparatus for detecting viable microorganisms or spores in a sample and use thereof.
DE102009043537B4 (de) * 2009-09-30 2012-03-22 Siemens Aktiengesellschaft Verfahren und Anordnung zur Bestimmung von Zell-Vitalitäten
CN106324052B (zh) * 2016-08-19 2019-07-19 李宗珍 一种检测压缩气体中的微生物的测试方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0441469A1 (de) * 1990-01-19 1991-08-14 Promega Corporation Immunspezifischer und Biolumineszenztest für zelluläres ATP
WO1992008805A1 (en) * 1990-11-15 1992-05-29 U-Gene Research B.V. Method and kit for demonstrating microorganisms
EP0605003A2 (de) * 1992-12-31 1994-07-06 Vicam, L.P. Testverfahren zum Nachweis der Anwesenheit von Bakterien
DE19714219A1 (de) * 1997-04-07 1998-10-08 Cornelius Prof Dr Friedrich Verfahren und Vorrichtung zur Aktivitätsbestimmung immobilisierter Mikroorganismen
WO2000044876A2 (de) * 1999-01-29 2000-08-03 Institut für Chemo- und Biosensorik Münster E.V. Verfahren, gefäss und vorrichtung zur überwachung der stoffwechselaktivität von zellkulturen in flüssigen medien

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994011078A1 (en) * 1992-11-16 1994-05-26 Immunivest Corporation Magnetic immobilization and manipulation of biological entities

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0441469A1 (de) * 1990-01-19 1991-08-14 Promega Corporation Immunspezifischer und Biolumineszenztest für zelluläres ATP
WO1992008805A1 (en) * 1990-11-15 1992-05-29 U-Gene Research B.V. Method and kit for demonstrating microorganisms
EP0605003A2 (de) * 1992-12-31 1994-07-06 Vicam, L.P. Testverfahren zum Nachweis der Anwesenheit von Bakterien
DE19714219A1 (de) * 1997-04-07 1998-10-08 Cornelius Prof Dr Friedrich Verfahren und Vorrichtung zur Aktivitätsbestimmung immobilisierter Mikroorganismen
WO2000044876A2 (de) * 1999-01-29 2000-08-03 Institut für Chemo- und Biosensorik Münster E.V. Verfahren, gefäss und vorrichtung zur überwachung der stoffwechselaktivität von zellkulturen in flüssigen medien

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Derwent World Patents Index; AN 1988-005166, XP002234086, B. MATIASSON: "Determination of microorganisms or monocellular bodies by measurement of metabolism..." *

Also Published As

Publication number Publication date
DE10293412D2 (de) 2004-07-01
WO2003012447A2 (de) 2003-02-13
DE10136472A1 (de) 2003-02-20
AU2002320995A1 (en) 2003-02-17

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