WO2001086285A3 - Direkter nachweis von einzelmolekülen - Google Patents

Direkter nachweis von einzelmolekülen Download PDF

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Publication number
WO2001086285A3
WO2001086285A3 PCT/EP2001/005408 EP0105408W WO0186285A3 WO 2001086285 A3 WO2001086285 A3 WO 2001086285A3 EP 0105408 W EP0105408 W EP 0105408W WO 0186285 A3 WO0186285 A3 WO 0186285A3
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WO
WIPO (PCT)
Prior art keywords
direct detection
individual molecules
microchannel
analyte
molecules
Prior art date
Application number
PCT/EP2001/005408
Other languages
English (en)
French (fr)
Other versions
WO2001086285A2 (de
Inventor
Rudolf Rigler
Original Assignee
Gnothis Holding Sa
Rudolf Rigler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gnothis Holding Sa, Rudolf Rigler filed Critical Gnothis Holding Sa
Priority to US10/240,788 priority Critical patent/US20040023229A1/en
Priority to EP01945119A priority patent/EP1281084A2/de
Priority to AU2001267428A priority patent/AU2001267428A1/en
Publication of WO2001086285A2 publication Critical patent/WO2001086285A2/de
Publication of WO2001086285A3 publication Critical patent/WO2001086285A3/de

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4412—Scattering spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
    • B01J2219/00286—Reactor vessels with top and bottom openings
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00279—Features relating to reactor vessels
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    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00414—Means for dispensing and evacuation of reagents using suction
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00436—Maskless processes
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00441—Maskless processes using lasers
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    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00511—Walls of reactor vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B01J2219/00277—Apparatus
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    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
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    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices

Abstract

Die Erfindung betrifft ein Verfahren zum direkten Nachweis eines Analyten in einer Probeflüssigkeit Während des Flusses in einem Mikrokanal und eine dafür geeignete Vorrichtung.
PCT/EP2001/005408 2000-05-12 2001-05-11 Direkter nachweis von einzelmolekülen WO2001086285A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/240,788 US20040023229A1 (en) 2000-05-12 2001-05-11 Direct detection of individual molecules
EP01945119A EP1281084A2 (de) 2000-05-12 2001-05-11 Direkter nachweis von einzelmolekülen
AU2001267428A AU2001267428A1 (en) 2000-05-12 2001-05-11 Direct detection of individual molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10023423A DE10023423B4 (de) 2000-05-12 2000-05-12 Direkter Nachweis von Einzelmolekülen
DE10023423.2 2000-05-12

Publications (2)

Publication Number Publication Date
WO2001086285A2 WO2001086285A2 (de) 2001-11-15
WO2001086285A3 true WO2001086285A3 (de) 2002-04-11

Family

ID=7641878

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/005408 WO2001086285A2 (de) 2000-05-12 2001-05-11 Direkter nachweis von einzelmolekülen

Country Status (5)

Country Link
US (1) US20040023229A1 (de)
EP (1) EP1281084A2 (de)
AU (1) AU2001267428A1 (de)
DE (1) DE10023423B4 (de)
WO (1) WO2001086285A2 (de)

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WO2009117033A2 (en) 2007-12-19 2009-09-24 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use
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CN202830041U (zh) * 2009-04-03 2013-03-27 Illumina公司 用于加热生物样本的设备
WO2011031377A1 (en) * 2009-09-09 2011-03-17 Helixis, Inc. Optical system for multiple reactions
AT508806B1 (de) * 2009-10-07 2013-06-15 Onkotec Gmbh Analysegerät und verfahren
DE102010049212A1 (de) * 2010-10-21 2012-04-26 Rudolf Grosskopf Simultane Fluoreszenzkorrelationsspektroskopie (sFCS)
KR102560611B1 (ko) * 2016-08-25 2023-07-27 엘지전자 주식회사 디스플레이 디바이스
EP3330763A1 (de) * 2016-12-05 2018-06-06 Gnothis AB Vorrichtung zur charakterisierung lumineszierender entitäten

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US20040023229A1 (en) 2004-02-05
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WO2001086285A2 (de) 2001-11-15
DE10023423B4 (de) 2009-03-05
EP1281084A2 (de) 2003-02-05

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