WO2003010185A3 - Procede pour produire des polypeptides acyles - Google Patents

Procede pour produire des polypeptides acyles Download PDF

Info

Publication number
WO2003010185A3
WO2003010185A3 PCT/DK2002/000501 DK0200501W WO03010185A3 WO 2003010185 A3 WO2003010185 A3 WO 2003010185A3 DK 0200501 W DK0200501 W DK 0200501W WO 03010185 A3 WO03010185 A3 WO 03010185A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptides
certain
lysine
host cells
transformed host
Prior art date
Application number
PCT/DK2002/000501
Other languages
English (en)
Other versions
WO2003010185A2 (fr
Inventor
Per Balschmidt
Ivan Diers
Michi Egel-Mitani
Jan Markussen
Thomas Hoeeg-Jensen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Priority to AU2002355160A priority Critical patent/AU2002355160A1/en
Priority to JP2003515544A priority patent/JP2005503141A/ja
Priority to EP02750841A priority patent/EP1421102A2/fr
Publication of WO2003010185A2 publication Critical patent/WO2003010185A2/fr
Publication of WO2003010185A3 publication Critical patent/WO2003010185A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/006—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length of peptides containing derivatised side chain amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1077—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons

Abstract

La présente invention concerne un procédé pour produire des polypeptides dans des cellules hôtes transformées, par expression d'une molécule précurseur du polypeptide souhaité, ces polypeptides devant être acylés au cours d'une étape in vitro ultérieure. La présente invention concerne également des séquences d'ADN, des vecteurs et des cellules hôtes transformées destinés à un usage dans ce procédé. En outre, cette invention concerne certains précurseurs des polypeptides souhaités et certains procédés d'acylation. Cette invention concerne un procédé pour produire des polypeptides qui sont de préférence acylés en certains groupes ε-amino de lysine.
PCT/DK2002/000501 2001-07-24 2002-07-18 Procede pour produire des polypeptides acyles WO2003010185A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2002355160A AU2002355160A1 (en) 2001-07-24 2002-07-18 Method for making acylated polypeptides
JP2003515544A JP2005503141A (ja) 2001-07-24 2002-07-18 アシル化ポリペプチドを作出するための方法
EP02750841A EP1421102A2 (fr) 2001-07-24 2002-07-18 Procede pour produire des polypeptides acyles

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200101140 2001-07-24
DKPA200101140 2001-07-24

Publications (2)

Publication Number Publication Date
WO2003010185A2 WO2003010185A2 (fr) 2003-02-06
WO2003010185A3 true WO2003010185A3 (fr) 2004-03-25

Family

ID=8160636

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2002/000501 WO2003010185A2 (fr) 2001-07-24 2002-07-18 Procede pour produire des polypeptides acyles

Country Status (4)

Country Link
EP (1) EP1421102A2 (fr)
JP (1) JP2005503141A (fr)
AU (1) AU2002355160A1 (fr)
WO (1) WO2003010185A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8158586B2 (en) 2005-04-11 2012-04-17 Pharmagap Inc. Inhibitors of protein kinases and uses thereof
EP2298792A3 (fr) * 2005-08-05 2011-10-19 Pharmagap Inc. Peptides ciblés pour les isoformes de protéine kinase C et utilisations associées
EP1872790A1 (fr) 2006-06-26 2008-01-02 DeveloGen Aktiengesellschaft Nouvelle formulation pour augmenter la biodisponibilité de neurturine
US20100317057A1 (en) 2007-12-28 2010-12-16 Novo Nordisk A/S Semi-recombinant preparation of glp-1 analogues

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995035384A1 (fr) * 1994-06-17 1995-12-28 Novo Nordisk A/S Proteines comportant une extension a l'extremite n-terminale, exprimees par une levure
EP0712862A2 (fr) * 1994-11-17 1996-05-22 Eli Lilly And Company Procédé d'acylation sélective des groupements d'amino-épsilon
US5905140A (en) * 1996-07-11 1999-05-18 Novo Nordisk A/S, Novo Alle Selective acylation method
US6011007A (en) * 1993-09-17 2000-01-04 Novo Nordisk A/S Acylated insulin
WO2000055119A1 (fr) * 1999-03-17 2000-09-21 Novo Nordisk A/S Methode d'acylation de peptides et nouveaux agents d'acylation

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6011007A (en) * 1993-09-17 2000-01-04 Novo Nordisk A/S Acylated insulin
WO1995035384A1 (fr) * 1994-06-17 1995-12-28 Novo Nordisk A/S Proteines comportant une extension a l'extremite n-terminale, exprimees par une levure
EP0712862A2 (fr) * 1994-11-17 1996-05-22 Eli Lilly And Company Procédé d'acylation sélective des groupements d'amino-épsilon
US5905140A (en) * 1996-07-11 1999-05-18 Novo Nordisk A/S, Novo Alle Selective acylation method
WO2000055119A1 (fr) * 1999-03-17 2000-09-21 Novo Nordisk A/S Methode d'acylation de peptides et nouveaux agents d'acylation

Also Published As

Publication number Publication date
WO2003010185A2 (fr) 2003-02-06
JP2005503141A (ja) 2005-02-03
EP1421102A2 (fr) 2004-05-26
AU2002355160A1 (en) 2003-02-17

Similar Documents

Publication Publication Date Title
HUP0301723A2 (hu) Eljárás peptidek előállítására in vitro transzkripciós/transzlációs rendszer alkalmazásával
EP2065465A3 (fr) Procédé, construction et conjugué pour la préparation de plusieurs copies d'acides nucléiques
WO2002044362A1 (fr) Procede de depistage d'un remede
WO2006017325A3 (fr) Compositions de vecteurs aav et procédés pour l'expression accrue d'immunoglobulines utilisant celles-ci
WO2003014318A3 (fr) Procede pour traiter le diabete et d'autres troubles lies a la glycemie
CA2454264A1 (fr) Methode de production d'un polypeptide insulinotropique glp-1 (7-36) et/ou d'analogues de glp-1
WO2007011975A3 (fr) Methodes d'expression de proteines de recombinaison a partir de vecteurs lentiviraux
WO2003010186A3 (fr) Procede de production de polypeptides acyles
WO2003010185A3 (fr) Procede pour produire des polypeptides acyles
PL1675956T3 (pl) Minimalna sekwencja DNA działająca jako insulator chromatyny i jej zastosowanie do wyrażania białek
WO2003062370A3 (fr) Proteines multimeres et methodes de production et d'utilisation de ces proteines
WO2001047956A3 (fr) Procede permettant d'obtenir des proteines possedant de meilleures caracteristiques fonctionnelles
HK1054410A1 (en) In vitro evolution of nucleic acids and encoded polypeptide
HK1079798A1 (en) Process for decreasing aggregate levels of pegylated protein
JP2001514189A (ja) コラーゲン擬似物
WO2003040391A3 (fr) Procede de production et d'identification de domaines proteiques solubles
WO2002004630A3 (fr) Procédés de synthèse recombinatoire d"acides nucléiques
ATE503003T1 (de) Verfahren zur herstellung rekombinanter polypeptide
ZA9610456B (en) N-terminally extended proteins expressed in yeast
WO2003099854A3 (fr) Procede de production enzymatique de peptides glp-2 (1-34) et glp-2 (1-33)
AU2001240540A1 (en) Method for the manufacture of recombinant trypsin
CA2369973A1 (fr) Production de procarboxypeptidase b pancreatique, de ses isoformes et muteines et leur utilisation
EP0818537A3 (fr) Procédé de clonage et de production de l'endonucléase SapI dans E. coli
ATE380863T1 (de) Neue, aus bacillus licheniformis stammende aminopeptidase, die aminopeptidase codierendes gen, das gen enthaltender expressionsvektor, transformant sowie verfahren zur herstellung davon
AU2001247842A1 (en) A promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producingfungus rhizopus oryzae and a method of expressing a gene of interest in fungal species

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BY BZ CA CH CN CO CR CU CZ DE DM DZ EC EE ES FI GB GD GE GH HR HU ID IL IN IS JP KE KG KP KR LC LK LR LS LT LU LV MA MD MG MN MW MX MZ NO NZ OM PH PL PT RU SD SE SG SI SK SL TJ TM TN TR TZ UA UG UZ VN YU ZA ZM

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LU MC NL PT SE SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ UG ZM ZW AM AZ BY KG KZ RU TJ TM AT BE BG CH CY CZ DK EE ES FI FR GB GR IE IT LU MC PT SE SK TR BF BJ CF CG CI GA GN GQ GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2002750841

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2003515544

Country of ref document: JP

WWP Wipo information: published in national office

Ref document number: 2002750841

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642