WO2003004697A3 - Verfahren zur detektion von mutagenen substanzen - Google Patents

Verfahren zur detektion von mutagenen substanzen Download PDF

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Publication number
WO2003004697A3
WO2003004697A3 PCT/EP2002/006990 EP0206990W WO03004697A3 WO 2003004697 A3 WO2003004697 A3 WO 2003004697A3 EP 0206990 W EP0206990 W EP 0206990W WO 03004697 A3 WO03004697 A3 WO 03004697A3
Authority
WO
WIPO (PCT)
Prior art keywords
revertants
mutations
developing
selection marker
reporter system
Prior art date
Application number
PCT/EP2002/006990
Other languages
English (en)
French (fr)
Other versions
WO2003004697A2 (de
Inventor
Georg Reifferscheid
Claudia Schmid
Original Assignee
Merck Patent Gmbh
Georg Reifferscheid
Claudia Schmid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh, Georg Reifferscheid, Claudia Schmid filed Critical Merck Patent Gmbh
Priority to JP2003510455A priority Critical patent/JP2004533260A/ja
Priority to AU2002310769A priority patent/AU2002310769A1/en
Priority to EP02735423A priority patent/EP1402057A2/de
Priority to US10/482,790 priority patent/US20050070006A1/en
Publication of WO2003004697A2 publication Critical patent/WO2003004697A2/de
Publication of WO2003004697A3 publication Critical patent/WO2003004697A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/142—Toxicological screening, e.g. expression profiles which identify toxicity

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Die Erfindung betrifft ein Verfahren und Mittel zum Schnellen Nachweis von Mutationen. Die erfindungsgemäss verwendeten bakteriellen Teststämme besitzen ein Reportersystem und einen Selektionsmarker. Als Selektionsmarker dient ein (Antibiotikum) Resistenzprotein, welches nach Mutation das selektive Wachstum der Revertanten und die gezielte Induktion des induzierbaren Reportersystems der wachsenden Revertanten ermöglicht. Als Reporter werden Proteine verwendet, die direkt oder indirekt ein Messsignal auslösen können. Der Nachweis der Mutationen erfolgt über das Reportersignal der wachsenden Revertanten, so dass die Auswertung bereits nach wenigen Stunden erfolgen kann.
PCT/EP2002/006990 2001-07-04 2002-06-25 Verfahren zur detektion von mutagenen substanzen WO2003004697A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2003510455A JP2004533260A (ja) 2001-07-04 2002-06-25 変異原性物質の検出方法
AU2002310769A AU2002310769A1 (en) 2001-07-04 2002-06-25 Method for detecting mutagenic substances
EP02735423A EP1402057A2 (de) 2001-07-04 2002-06-25 Verfahren zur detektion von mutagenen substanzen
US10/482,790 US20050070006A1 (en) 2001-07-04 2002-06-25 Method for detecting mutagenic substances

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10132280A DE10132280A1 (de) 2001-07-04 2001-07-04 Verfahren zur Detektion von mutagenen Substanzen
DE10132280.1 2001-07-04

Publications (2)

Publication Number Publication Date
WO2003004697A2 WO2003004697A2 (de) 2003-01-16
WO2003004697A3 true WO2003004697A3 (de) 2004-01-22

Family

ID=7690494

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/006990 WO2003004697A2 (de) 2001-07-04 2002-06-25 Verfahren zur detektion von mutagenen substanzen

Country Status (6)

Country Link
US (1) US20050070006A1 (de)
EP (1) EP1402057A2 (de)
JP (1) JP2004533260A (de)
AU (1) AU2002310769A1 (de)
DE (1) DE10132280A1 (de)
WO (1) WO2003004697A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11339420B2 (en) 2017-01-01 2022-05-24 The Regents Of The University Of California Fluorescence-based reporters for mutagenesis detection in E. coli
CN106636164A (zh) * 2017-01-18 2017-05-10 华南理工大学 一种遗传毒性物质检测载体及检测方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0649905A1 (de) * 1993-10-22 1995-04-26 Kabushiki Kaisha Toyota Chuo Kenkyusho Verfahren zum Nachweis von Mutagenen unter Verwendung eines Lumineszensgens
EP1217077A2 (de) * 2000-12-22 2002-06-26 Pfizer Products Inc. Neue bioluminiszente Untersuchungsverfahren sowie in diesen verwendbare Bakterienstämme

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0649905A1 (de) * 1993-10-22 1995-04-26 Kabushiki Kaisha Toyota Chuo Kenkyusho Verfahren zum Nachweis von Mutagenen unter Verwendung eines Lumineszensgens
EP1217077A2 (de) * 2000-12-22 2002-06-26 Pfizer Products Inc. Neue bioluminiszente Untersuchungsverfahren sowie in diesen verwendbare Bakterienstämme

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BOSWORTH D ET AL: "A FORWARD MUTATION ASSAY USING AMPICILLIN-RESISTANCE IN ESCHERICHIA-COLI DESIGNED FOR INVESTIGATING THE MUTAGENICITY OF BIOLOGICAL SAMPLES", MUTAGENESIS, vol. 2, no. 6, 1987, pages 455 - 468, XP009014185, ISSN: 0267-8357 *
JOSEPHY P DAVID: "The Escherichia coli lacZ reversion mutagenicity assay.", MUTATION RESEARCH, vol. 455, no. 1-2, 2000, pages 71 - 80, XP002249454, ISSN: 0027-5107 *
LEE C-C ET AL: "A REVERSE MUTAGENICITY ASSAY FOR ALKYLATING AGENTS BASED ON A POINT MUTATION IN THE BETA-LACTAMASE GENE AT THE ACTIVE SITE SERINE CODON", MUTAGENESIS, IRL PRESS, OXFORD, GB, vol. 9, no. 5, 1994, pages 401 - 405, XP009011129, ISSN: 0267-8357 *

Also Published As

Publication number Publication date
DE10132280A1 (de) 2003-01-23
WO2003004697A2 (de) 2003-01-16
AU2002310769A1 (en) 2003-01-21
EP1402057A2 (de) 2004-03-31
JP2004533260A (ja) 2004-11-04
US20050070006A1 (en) 2005-03-31

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