WO2002088673A3 - Detector for airborne biological particles - Google Patents

Detector for airborne biological particles Download PDF

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Publication number
WO2002088673A3
WO2002088673A3 PCT/GB2002/002027 GB0202027W WO02088673A3 WO 2002088673 A3 WO2002088673 A3 WO 2002088673A3 GB 0202027 W GB0202027 W GB 0202027W WO 02088673 A3 WO02088673 A3 WO 02088673A3
Authority
WO
WIPO (PCT)
Prior art keywords
particles
source
detector
airborne
biological particles
Prior art date
Application number
PCT/GB2002/002027
Other languages
French (fr)
Other versions
WO2002088673A2 (en
Inventor
Paul Henry Kaye
Original Assignee
Univ Hertfordshire
Paul Henry Kaye
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0110762A external-priority patent/GB0110762D0/en
Priority claimed from GB0206157A external-priority patent/GB0206157D0/en
Application filed by Univ Hertfordshire, Paul Henry Kaye filed Critical Univ Hertfordshire
Publication of WO2002088673A2 publication Critical patent/WO2002088673A2/en
Publication of WO2002088673A3 publication Critical patent/WO2002088673A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state
    • G01N1/24—Suction devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
    • G01N15/1427—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement with the synchronisation of components, a time gate for operation of components, or suppression of particle coincidences
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/22—Devices for withdrawing samples in the gaseous state
    • G01N1/2202—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling
    • G01N2001/222—Other features
    • G01N2001/2223—Other features aerosol sampling devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/019—Biological contaminants; Fouling
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1477—Multiparameters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Signal Processing (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

This invention describes a method and apparatus for the detection of airborne biological particles. The detection is based upon the measurement of fluorescence spectra produced by individual airborne particles when they are excited by a single or a sequence of two or more wavelengths of electromagnetic radiation. The apparatus in one aspect comprises a zone through which air to be analysed flows, in use; a source of illumination to illuminate / irradiate airborne particles present in the zone and to excite the particles to fluoresce, the source being a source of non-monochromatic light; and a detector to detect fluorescence from the particles as an indicator of the presence of biological particles. The source is suitably a pulsed xenon source and the detector an array that captures linear spectra for each particle in the airflow.
PCT/GB2002/002027 2001-05-02 2002-05-02 Detector for airborne biological particles WO2002088673A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0110762A GB0110762D0 (en) 2001-05-02 2001-05-02 Low cost monitor/detector for airborne biological particles
GB0110762.2 2001-05-02
GB0206157.0 2002-03-15
GB0206157A GB0206157D0 (en) 2002-03-15 2002-03-15 A fluorescence spectrum system for detection of airborne biological particles

Publications (2)

Publication Number Publication Date
WO2002088673A2 WO2002088673A2 (en) 2002-11-07
WO2002088673A3 true WO2002088673A3 (en) 2003-02-20

Family

ID=26246027

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/002027 WO2002088673A2 (en) 2001-05-02 2002-05-02 Detector for airborne biological particles

Country Status (2)

Country Link
GB (1) GB2378752A (en)
WO (1) WO2002088673A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2419406B (en) * 2003-06-26 2007-04-18 Secr Defence Improvements to fluid borne particle analysers
GB0326669D0 (en) * 2003-11-15 2003-12-17 Partrac Ltd Apparatus for collecting fluid borne particles
DE102004031197A1 (en) * 2004-06-28 2006-01-19 Schweiger, Gustav, Prof. Dipl.-Ing. Dr.techn. Biological origin substance small quantities identification device measures angle of dependence of light diffused in-elastically on bio-material and compares with reference measurements
US7772579B2 (en) 2006-05-18 2010-08-10 Massachusetts Institute Of Technology Method and apparatus for simultaneously measuring a three dimensional position of a particle in a flow
WO2008010870A2 (en) 2006-05-18 2008-01-24 Massachusetts Institute Of Technology Method and apparatus for measuring a position of a particle in a flow
US9546953B2 (en) 2007-07-30 2017-01-17 Spherea Gmbh Method and apparatus for real-time analysis of chemical, biological and explosive substances in the air
JP5647108B2 (en) * 2008-06-10 2014-12-24 エックストラリス・テクノロジーズ・リミテッド Particle detection system and particle detection method using particle detector
CN101813628B (en) * 2010-04-08 2012-07-25 长春理工大学 Manufacture method of ultraviolet biological chip integrated sensor
FI20105645A0 (en) * 2010-06-07 2010-06-07 Environics Oy APPARATUS AND METHOD FOR DETECTING BIOLOGICAL MATERIAL
CN103095200B (en) * 2012-12-28 2015-11-04 中国科学院微电子研究所 A kind of whirligig and the single-particle test macro based on this whirligig
KR102203201B1 (en) 2014-02-27 2021-01-14 엘지전자 주식회사 An air cleaning system and a method controlling the same
CN106680249B (en) * 2015-11-10 2019-03-19 中国科学院大连化学物理研究所 The online microalgae chlorophyll fluorescence measurement module of flow type

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5110204A (en) * 1990-11-06 1992-05-05 Trustees Of Princeton University Velocity measurement by the vibrational tagging of diatomic molecules
US5434667A (en) * 1988-09-15 1995-07-18 Eastman Kodak Company Characterization of particles by modulated dynamic light scattering
US5491642A (en) * 1993-12-03 1996-02-13 United Technologies Corporation CCD based particle image direction and zero velocity resolver
WO2000019195A1 (en) * 1998-09-29 2000-04-06 The United States Of America, Represented By The Secretary Of The Army Environmental material ticket reader (emtr) and environmental material ticket (emt) system
EP1158292A2 (en) * 2000-05-23 2001-11-28 Wyatt Technology Corporation Aerosol hazard characterization and early warning network

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62111680A (en) * 1985-11-11 1987-05-22 Matsushita Seiko Co Ltd Counter for microorganism
JPS63247643A (en) * 1987-04-02 1988-10-14 Kondo Kogyo Kk Method for measuring suspended bacteria
US5422719A (en) * 1993-11-12 1995-06-06 Auburn International, Inc. Multi-wave-length spectrofluorometer
WO1998041876A1 (en) * 1997-03-17 1998-09-24 Tsi Incorporated System for detecting fluorescing components in aerosols
US6194731B1 (en) * 1998-11-12 2001-02-27 The United States Of America As Represented By The Secretary Of The Air Force Bio-particle fluorescence detector
US6249341B1 (en) * 1999-01-25 2001-06-19 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5434667A (en) * 1988-09-15 1995-07-18 Eastman Kodak Company Characterization of particles by modulated dynamic light scattering
US5110204A (en) * 1990-11-06 1992-05-05 Trustees Of Princeton University Velocity measurement by the vibrational tagging of diatomic molecules
US5491642A (en) * 1993-12-03 1996-02-13 United Technologies Corporation CCD based particle image direction and zero velocity resolver
WO2000019195A1 (en) * 1998-09-29 2000-04-06 The United States Of America, Represented By The Secretary Of The Army Environmental material ticket reader (emtr) and environmental material ticket (emt) system
EP1158292A2 (en) * 2000-05-23 2001-11-28 Wyatt Technology Corporation Aerosol hazard characterization and early warning network

Also Published As

Publication number Publication date
WO2002088673A2 (en) 2002-11-07
GB2378752A (en) 2003-02-19
GB0210116D0 (en) 2002-06-12

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