JPS63247643A - Method for measuring suspended bacteria - Google Patents
Method for measuring suspended bacteriaInfo
- Publication number
- JPS63247643A JPS63247643A JP62079788A JP7978887A JPS63247643A JP S63247643 A JPS63247643 A JP S63247643A JP 62079788 A JP62079788 A JP 62079788A JP 7978887 A JP7978887 A JP 7978887A JP S63247643 A JPS63247643 A JP S63247643A
- Authority
- JP
- Japan
- Prior art keywords
- bacteria
- air
- sample
- sample air
- pulses
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims description 7
- 238000002189 fluorescence spectrum Methods 0.000 claims description 4
- 230000005284 excitation Effects 0.000 abstract description 7
- 238000005259 measurement Methods 0.000 abstract description 3
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 abstract description 3
- 229910052753 mercury Inorganic materials 0.000 abstract description 3
- 238000001228 spectrum Methods 0.000 abstract 3
- 238000005070 sampling Methods 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 description 7
- 239000002245 particle Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000009474 immediate action Effects 0.000 description 1
- 238000006241 metabolic reaction Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
本発明は、空気中に浮遊する細菌のｈ↑測方法に関する
ものである。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a method for measuring h↑ of bacteria floating in the air.
［従来の技術］
従来、空気中に浮″ｆ１″ｇる細菌を測定する手段とし
ては、例えばスリットナンプラー、アンダーセン瞥ナン
プラー等があり、それ等は吸引採取した空気を培養基を
入れたシャーレに吹付けて、サンプル空気中の細菌を培
養し、培養した細菌のコロニー数を測定するものである
。[Prior Art] Conventionally, as a means of measuring bacteria floating in the air, there are, for example, a slit sample plate and an Andersen plate sample plate, which blow collected air into a petri dish containing a culture medium. It is used to culture bacteria in sample air and measure the number of colonies of cultured bacteria.
［発明が解決しようとする問題点］
ところで、前記従来の如く採取したサンプル空気中の細
菌を培養するためには、２４時間〜４８時間を要し、細
菌のコロニー数を測定するまでに時間がかかりすぎると
いう問題点があり、このことは環境改善のための対応措
置を直ちに行なうことができないということになる。[Problems to be Solved by the Invention] By the way, it takes 24 to 48 hours to culture the bacteria in the air sample collected in the conventional manner, and it takes a long time to measure the number of bacterial colonies. The problem is that it takes too long, which means that immediate action cannot be taken to improve the environment.
［問題点を解決するための手段］
本発明は、前記従来の問題点を解決すべくなされたもの
であって、その要旨とするところは、所定の吸引量にて
採取中のナンプル空気に紫外線を照射して、サンプル空
気中の細菌を励起蛍光発光させ、このときの蛍光スペク
トルを電気信号に変換し、この変換された電気信号にお
けるパルス数と、パルス波高値と、パルス幅とから、サ
ンプル空気中の細菌を計測することにある。[Means for Solving the Problems] The present invention has been made to solve the above-mentioned conventional problems, and the gist thereof is to apply ultraviolet rays to the sample air being collected at a predetermined suction amount. The bacteria in the sample air are irradiated to excite the bacteria to emit fluorescence, and the fluorescence spectrum at this time is converted into an electrical signal.The number of pulses, pulse height value, and pulse width of the converted electrical signal are used to determine the sample size. The purpose is to measure bacteria in the air.
し作用］
細菌は主成分がほとんど蛋白質であって、代謝反応をも
った有は物であり、本発明は紫外線を照射したときの物
質の残光特性と、蛍光スペクトル分析とによって一義的
に定まるパルス数と、パルス波高値と、パルス幅とから
、サンプル空気中の細菌数をリアルタイムに胴側できる
。Bacteria are creatures whose main components are mostly proteins and have metabolic reactions, and the present invention is uniquely determined by the afterglow characteristics of substances when irradiated with ultraviolet rays and fluorescence spectrum analysis. The number of bacteria in the sample air can be determined in real time from the pulse number, pulse height value, and pulse width.
［実施例］ 次に本発明方法の実施例を図面に基づいて説明する。[Example] Next, an embodiment of the method of the present invention will be described based on the drawings.
第１図に示す如く、超高圧水銀灯１からの光を集光レン
ズ２にて集光すると共に、励起フィルター３を介して、
サンプル空気導入管路４の中心線と直交するように、サ
ンプル空気導入管路４中における紫外線励起部５へ焦点
を合わせる。なお、６は光トラップである。As illustrated in FIG.
The ultraviolet ray excitation section 5 in the sample air introduction pipe 4 is focused so as to be perpendicular to the center line of the sample air introduction pipe 4. Note that 6 is an optical trap.
一方、前記ナンプル空気導入管路４の管路端末４ｂに、
流ｌＨｆ７、ニードルバルブ８を介して吸引ポンプ９を
連結し、サンプル空気導入管路４の′す”ンプル空気導
入口４ａから前記紫外線励起部５内へ、外部、例えば室
内の空気を吸引し、この吸引したサンプル空気に紫外線
を照射して、サンプル空気中の細菌を励起蛍光発光させ
る。On the other hand, at the pipe terminal 4b of the Nampuru air introduction pipe 4,
A suction pump 9 is connected to the flow lHf7 through a needle valve 8, and external, for example, indoor air is sucked into the ultraviolet excitation unit 5 from the sample air introduction port 4a of the sample air introduction conduit 4. The sucked sample air is irradiated with ultraviolet light to excite bacteria in the sample air to cause them to emit fluorescence.
そして、第２図に示す如く、前記紫外線励起部５におけ
る光軸上の焦点位置と直角な、散乱光の影響を受けない
位置に設置された集光レンズ１０によって、前記励起蛍
光発光された細菌からの蛍光スペクトルを集光すると共
に、分光器１１に導入して分光し、更にフォトマルチプ
ライヤ−１２により電気信号に変換し、この変換された
電気信号を増幅器１３を介して波形分析器１４へ入力さ
け、この波形分析器１４によりパルス数と、パルス波高
値と、パルス幅とを同時に測定し、これ等パルス数と、
パルス波高値と、パルス幅との測定値から、サンプル空
気中の細菌をバ゛１測する。As shown in FIG. 2, the bacteria emitted from the excited fluorescent light are detected by a condenser lens 10 installed at a position not affected by scattered light and perpendicular to the focal position on the optical axis of the ultraviolet excitation unit 5. At the same time, the fluorescence spectra from Upon input, the waveform analyzer 14 simultaneously measures the number of pulses, the pulse height value, and the pulse width, and calculates the number of pulses and the pulse width.
Bacteria in the sample air are determined from the pulse height and pulse width measurements.
実験例
試験的大腸菌発生源（３，０ｘ１０８個／ｄ＞から大腸
菌を閉空間に粒子として発生させ、この大腸菌の粒子を
含む空気をアンダーセンサンプラ−（吸引量２８．３℃
／―）とスリットリンプラー（吸引量３３．３℃／―）
で採取し、細菌数を測定したところ、６７個／１００ｆ
ｌ〜１０２５個／１００４！であった。Experimental Example Escherichia coli was generated as particles in a closed space from a test source of Escherichia coli (3,0 x 108 particles/d), and the air containing the Escherichia coli particles was collected using an Andersen sampler (suction volume 28.3°C).
/-) and slit rinser (suction amount 33.3℃/-)
When the number of bacteria was measured, it was 67/100f.
l~1025 pieces/1004! Met.
同一の大腸菌発生源（３，０ＸＩＯ８個／ｄ）から本発
明方法の実施例におけるサンプル空気導入管路へ大腸菌
（生菌）を吸引１２５２７分にて導入し、超高圧水銀灯
（２５０Ｗ＞の光により大腸菌を励起蛍光発光させ、フ
ォトマルチプライヤ−の印加電圧を一４００Ｖにし、波
形分析器（オシロスコープ）にて１分間のパルス数を目
視により胴側したところ、約５０〜６０パルスであった
。Escherichia coli (live bacteria) was sucked into the sample air introduction conduit in the example of the method of the present invention from the same source of Escherichia coli (3,0XIO8 pieces/d) for 12,527 minutes, and then exposed to light from an ultra-high pressure mercury lamp (250 W). E. coli was excited to emit fluorescence, the voltage applied to the photomultiplier was set to 1,400 V, and the number of pulses per minute was visually measured using a waveform analyzer (oscilloscope), and the number was approximately 50 to 60 pulses.
このパルス数を１００ｆｔ中の大腸菌数に換算すると、
約２００個／１００λ〜２４０個／１００２となり、前
記アンダーセンサンプラー、スリットサンプラーによる
場合の測定値内にあることが確認された。Converting this number of pulses to the number of E. coli in 100ft is:
It was confirmed that the value was about 200 pieces/100λ to 240 pieces/1002, which was within the measurement values obtained using the Andersen sampler and slit sampler.
「発明の効果］
以上述べた如く、本発明に係る浮遊細菌の計測方法にＪ
：れば、採取したサンプル空気中の細菌数をリアルタイ
ムに計測できるので、環境改善のための対応措置を直ち
に行なうことができる。“Effects of the Invention” As stated above, the method for measuring airborne bacteria according to the present invention
: Since the number of bacteria in the sampled air can be measured in real time, countermeasures can be taken immediately to improve the environment.
第１図は本発明方法の実施例を示す正面図的ブロック図
、第２図は同上の平面図的ブロック図である。
１・・・・・・・・・超高圧水銀灯、２・・・・・・・
・・集光レンズ、訃・・・・・・・・励起フィルター、
４・・・・・・・・・サンプル空気導入管路、５・・・
・・・・・・紫外線励起部、６・・・・・・・・・光ト
ラップ、７・・・・・・・・・流量計、 ８・・・・・
・・・・ニードルバルブ、９・・・・・・・・・吸引ポ
ンプ、 １０・・・・・・集光レンズ、１１・・・・・
・分光器、
１２・・・・・・フォトマルチプライヤー、１３・・・
・・・増幅器、 １４・・・・・・波形分析器昭和６２
年 ４月 ２日FIG. 1 is a front block diagram showing an embodiment of the method of the present invention, and FIG. 2 is a plan block diagram of the same. １・・・・・・・・・Ultra high pressure mercury lamp, ２・・・・・・・・・
...Condensing lens, Excitation filter, 4... Sample air introduction pipe, 5...
......Ultraviolet excitation section, 6......Light trap, 7...Flowmeter, 8...
...Needle valve, 9...Suction pump, 10...Condensing lens, 11...
・Spectrometer, 12...Photomultiplier, 13...
...Amplifier, 14...Waveform analyzer 1982
April 2nd
Claims (1)
して、サンプル空気中の細菌を励起蛍光発光させ、この
ときの蛍光スペクトルを電気信号に変換し、この変換さ
れた電気信号におけるパルス数と、パルス波高値と、パ
ルス幅とから、サンプル空気中の細菌を計測することを
特徴とする浮遊細菌の計測方法。The sample air being collected is irradiated with ultraviolet rays at a predetermined amount of suction, the bacteria in the sample air are excited and emit fluorescence, the fluorescence spectrum at this time is converted into an electrical signal, and the number of pulses in this converted electrical signal is calculated. A method for measuring airborne bacteria, characterized by measuring bacteria in sample air from pulse wave height values and pulse widths.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62079788A JPS63247643A (en) | 1987-04-02 | 1987-04-02 | Method for measuring suspended bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62079788A JPS63247643A (en) | 1987-04-02 | 1987-04-02 | Method for measuring suspended bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS63247643A true JPS63247643A (en) | 1988-10-14 |
Family
ID=13699952
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62079788A Pending JPS63247643A (en) | 1987-04-02 | 1987-04-02 | Method for measuring suspended bacteria |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63247643A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2378752A (en) * | 2001-05-02 | 2003-02-19 | Univ Hertfordshire | Optical detection of the individual airborne biological particles |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5766342A (en) * | 1980-04-28 | 1982-04-22 | Agency Of Ind Science & Technol | Optical measuring method for suspension particles in medium |
| JPS60230040A (en) * | 1984-04-27 | 1985-11-15 | Mitsubishi Electric Corp | Method for measuring number of mathane bacteria and methane forming activity |
-
1987
- 1987-04-02 JP JP62079788A patent/JPS63247643A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5766342A (en) * | 1980-04-28 | 1982-04-22 | Agency Of Ind Science & Technol | Optical measuring method for suspension particles in medium |
| JPS60230040A (en) * | 1984-04-27 | 1985-11-15 | Mitsubishi Electric Corp | Method for measuring number of mathane bacteria and methane forming activity |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2378752A (en) * | 2001-05-02 | 2003-02-19 | Univ Hertfordshire | Optical detection of the individual airborne biological particles |
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