WO2002059353A3 - Amplification par reaction en chaine de la polymerase (pcr) asymetrique - Google Patents

Amplification par reaction en chaine de la polymerase (pcr) asymetrique Download PDF

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Publication number
WO2002059353A3
WO2002059353A3 PCT/CA2002/000057 CA0200057W WO02059353A3 WO 2002059353 A3 WO2002059353 A3 WO 2002059353A3 CA 0200057 W CA0200057 W CA 0200057W WO 02059353 A3 WO02059353 A3 WO 02059353A3
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WO
WIPO (PCT)
Prior art keywords
amplification
dna
reaction
approach
sequencing
Prior art date
Application number
PCT/CA2002/000057
Other languages
English (en)
Other versions
WO2002059353A2 (fr
Inventor
Qinyin Cai
Peng Ling
Jin-Hao Liu
Original Assignee
Bio S & T
Qinyin Cai
Peng Ling
Jin-Hao Liu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA002332610A external-priority patent/CA2332610A1/fr
Application filed by Bio S & T, Qinyin Cai, Peng Ling, Jin-Hao Liu filed Critical Bio S & T
Priority to AU2002231489A priority Critical patent/AU2002231489A1/en
Publication of WO2002059353A2 publication Critical patent/WO2002059353A2/fr
Publication of WO2002059353A3 publication Critical patent/WO2002059353A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne une technique d'amplification d'ADN qui permet une amplification de cibles d'ADN à séquences inconnues, comprenant des étapes de PCR successives. Dans une première réaction, on réalise l'amplification linéaire de l'acide nucléique cible en utilisant une ou plusieurs amorces spécialement prévues. Dans une deuxième réaction, on ajoute des amorces additionnelles et on réalise l'amplification exponentielle d'ADN double brin. Associée à des procédures de séquençage classiques, on peut utiliser cette technique pour un séquençage direct d'ADN génomique, ce qui évite d'avoir à sous-cloner de gros fragments. On peut aussi, grâce à cette technique rassembler des informations de séquençage à coûts bien moindre et avec beaucoup moins de main d'oeuvre qu'auparavant.
PCT/CA2002/000057 2001-01-26 2002-01-16 Amplification par reaction en chaine de la polymerase (pcr) asymetrique WO2002059353A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002231489A AU2002231489A1 (en) 2001-01-26 2002-01-16 Two-step amplification using a program primer followed by specific primers

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CA2,332,610 2001-01-26
CA002332610A CA2332610A1 (fr) 2001-01-26 2001-01-26 Amplification asymetrique de la reaction en chaine de la polymerase
US09/825,873 US20020155448A1 (en) 2001-01-26 2001-04-05 Asymmetrical PCR amplification
US09/825,873 2001-04-05

Publications (2)

Publication Number Publication Date
WO2002059353A2 WO2002059353A2 (fr) 2002-08-01
WO2002059353A3 true WO2002059353A3 (fr) 2003-08-21

Family

ID=25682361

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2002/000057 WO2002059353A2 (fr) 2001-01-26 2002-01-16 Amplification par reaction en chaine de la polymerase (pcr) asymetrique

Country Status (1)

Country Link
WO (1) WO2002059353A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10253337B4 (de) * 2002-11-14 2005-10-20 November Ag Molekulare Medizin Verfahren zum Nachweis einer Nukleinsäure
WO2006094360A1 (fr) * 2005-03-11 2006-09-14 Molecular Plant Breeding Nominees Ltd Procede destine a amplifier les acides nucleiques
EP1947196A4 (fr) * 2005-11-08 2009-09-09 Olympus Corp Procédé d'amplification de plusieurs séquences d'acides nucléiques en vue de leur différenciation
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
JP2008154467A (ja) * 2006-12-21 2008-07-10 Olympus Corp 核酸の増幅方法とこれを用いた核酸の解析方法
CN102732609B (zh) * 2011-04-08 2014-03-26 博奥生物有限公司 一种检测寡核苷酸与目的基因组的相似性的方法
HU229967B1 (hu) * 2011-12-20 2015-03-30 Kps Diagnosztika Zrt Eljárás töredezett nukleinsavak szekvenciájának meghatározására
US9139870B2 (en) 2012-08-30 2015-09-22 Gen-Probe Incorporated Multiphase nucleic acid amplification
GB201410646D0 (en) 2014-06-14 2014-07-30 Illumina Cambridge Ltd Methods of increasing sequencing accuracy
US10563196B2 (en) * 2014-10-17 2020-02-18 Mgi Tech Co., Ltd Primer for nucleic acid random fragmentation and nucleic acid random fragmentation method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5407799A (en) * 1989-09-14 1995-04-18 Associated Universities, Inc. Method for high-volume sequencing of nucleic acids: random and directed priming with libraries of oligonucleotides
WO1997030062A1 (fr) * 1996-02-14 1997-08-21 Akzo Nobel N.V. Isolation et amplification d'acides nucleiques
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5407799A (en) * 1989-09-14 1995-04-18 Associated Universities, Inc. Method for high-volume sequencing of nucleic acids: random and directed priming with libraries of oligonucleotides
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5731171A (en) * 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
WO1997030062A1 (fr) * 1996-02-14 1997-08-21 Akzo Nobel N.V. Isolation et amplification d'acides nucleiques

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BOOTH CLAIRE ET AL: "Quantitative amplification of single-stranded DNA (QAOS) demonstrates that cdc13-1 mutants generate ssDNA in a telomere to centromere direction.", NUCLEIC ACIDS RESEARCH, vol. 29, no. 21, 1 November 2001 (2001-11-01), pages 4414 - 4422, XP002242797, ISSN: 0305-1048 *
FRITZ J D ET AL: "A NOVEL 3' EXTENSION TECHNIQUE USING RANDOM PRIMERS IN RNA-PCR", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 19, no. 13, 11 July 1991 (1991-07-11), pages 3747, XP000371658, ISSN: 0305-1048 *
PODDAR SK: "Symmetric vs asymmetric PCR and molecular beacon probe in the detection of a target gene of adenovirus", MOLECULAR AND CELLULAR PROBES, ACADEMIC PRESS, LONDON, GB, vol. 14, February 2000 (2000-02-01), pages 25 - 32, XP000953250, ISSN: 0890-8508 *
TELENIUS H ET AL: "DEGENERATE OLIGONUCLEOTIDE-PRIMED PCR: GENERAL AMPLIFICATION OF TARGET DNA BY A SINGLE DEGENERATE PRIMER", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 13, 1992, pages 718 - 725, XP000199116, ISSN: 0888-7543 *
WELSH J ET AL: "FINGERPRINTING GENOMES USING PCR WITH ARBITRARY PRIMERS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 18, no. 24, 25 December 1990 (1990-12-25), pages 7213 - 7218, XP000310554, ISSN: 0305-1048 *

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