WO2002049416A1

WO2002049416A1 - Liquid spawn inoculation device

Info

Publication number: WO2002049416A1
Application number: PCT/JP2001/010562
Authority: WO
Inventors: Yuichi Maruyama; Masaji Osabe; Masahiko Fujisawa
Original assignee: Nippon Seiki Co.,Ltd
Priority date: 2000-12-19
Filing date: 2001-12-03
Publication date: 2002-06-27
Also published as: JP2002186354A; CN1404358A; KR20020079855A

Abstract

A liquid spawn inoculation device, comprising a transfer mechanism (2) for feeding a container (9) having cultivating containers (8) orderly stored therein in a plurality of rows in vertical and horizontal directions from an inlet side to an outlet side while intermittently feeding the cultivating containers (8), cover opening and closing mechanisms (12) opening and closing the covers (7) of the cultivating containers (8) in the container (9) fed intermittently by the transfer mechanism (2) for each lateral row and disposed, for each lateral row, in a plurality of rows apart specified distances from each other, and liquid spawn feed mechanisms (13) disposed in a plurality of rows to feed a specified amount of liquid spawn (B) into the cultivating containers (8) for each lateral row with the covers (7) opened by the cover opening and closing mechanisms (12) in the plurality of rows, whereby a stable spawn filling can be performed while increasing the efficiency of an inoculating operation and maintaining the amount of liquid spawn (B) fed into the cultivating containers (8) generally at a constant.

Description

Technical field

TECHNICAL FIELD The present invention relates to an inoculum inoculation device for inoculating a culture medium filled in a mushroom culture container with a mushroom inoculum. Background technology ''

Conventionally, as a method of artificially cultivating mushrooms using a culture medium containing a sawdust or rice bran as a main component and a nutrient source, a culture medium is filled into a cultivation container, sterilized, and the enoki mushroom is placed in the filled cultivation container. After inoculating seeds of mushrooms such as mushrooms and mushrooms, the cultivation container is covered with a lid and closed, and the bacteria are cultured under predetermined temperature and humidity conditions to industrially cultivate. Let's do it.

In this case, when inoculating the inoculum, the inoculation can be carried out by placing each container on a transport conveyor without removing the cultivation containers one by one from the container in order to increase the efficiency of the inoculation work. An apparatus has been proposed in, for example, Japanese Patent Application Laid-Open No. 63-25859 / 19 and Japanese Patent Application Laid-Open No. 63-258850 / 1988.

In this inoculation device, the inoculum itself is generally grown in a seed culture bottle in a culture medium containing nutrients mainly composed of sawdust and rice bran, and the inoculum is housed. The seed bottle is turned upside down and attached to the body frame side.In this state, the seed bottle is rotated, and the seed bottle is rotated while rotating the extraction shaft provided with the extraction blade from the mouth of the seed bottle. The seeds are extracted by the extraction blade while rotating the extraction shaft slowly while rotating the extraction shaft, and the seeds extracted into the culture vessel via a hopper etc. It is configured to be filled.

In addition, in the above-mentioned conventional technology, the filling amount of the inoculum generally sets the rotation time of the extraction shaft according to the type of the mushroom to be cultivated. Depending on the difference between the size and the outer diameter of the torso, the amount of inoculation of the inoculum may differ, and since the inoculum itself in the inoculum bottle is solid, The size of the inoculum (sawdust) ejected at the time of extraction may vary, and as a result, the amount of the inoculum may vary, particularly when the amount of the inoculum is less than a predetermined amount. In this case, it is expected that the hypha of the inoculum will not easily propagate in the cultivation container, and the culturing will take a long time.

In order to avoid this, it is conceivable to detect the amount of inoculated bacteria by using, for example, a photoelectric sensor to detect the amount of inoculated bacteria.However, the detection accuracy varies due to contamination of the sensor and its mounting portion. Some say that it will.

Further, in the above-described conventional technique, the lids of a plurality of cultivation containers are opened in a container in a column and a row, respectively, by lid opening / closing means, and the container is moved forward together with the cultivation containers while the lids are open. The seed is transported to the installation position of the inoculum filling mechanism, the inoculum extracted by the inoculum filling mechanism is filled in the cultivation container and inoculated, and after the inoculation, the lid is closed while the container is retracted and transferred. A series of inoculum inoculation work is completed through the process.

However, it takes time to switch between each process, such as opening the lid of the cultivation container, filling the inoculum, and closing the lid of the cultivation container.As a result, if the lid opening time is longer during inoculation of the inoculum, However, there is also a problem that germs floating in the external atmosphere invade the cultivation container, so that they can be easily removed.

Therefore, the present invention solves the above-mentioned problems of the conventional example, and aims to improve the efficiency of inoculation work while maintaining a stable supply of seed bacteria to be supplied to the cultivation container while maintaining a stable amount. It is an object of the present invention to provide a liquid inoculum inoculation device capable of performing the inoculation of the inoculum. Disclosure of the invention

In the invention according to the first aspect of the present invention, a transport mechanism for supplying a container containing a plurality of cultivation containers arranged in rows and columns from the loading side and transporting the containers to the unloading side while intermittently transferring the cultivation containers. The lids of the cultivation containers in the container intermittently transferred by the transport mechanism are opened and closed in units of horizontal rows, and the lids arranged in a plurality of rows at predetermined intervals with the horizontal row units as a set. An opening / closing mechanism, and a predetermined amount of liquid inoculum is placed in front of each horizontal row of cultivation containers whose lids are opened by the plural rows of lid opening / closing mechanisms. With the provision of a liquid seed supply mechanism arranged in a plurality of rows that respectively supply the cultivation containers, the lids of the cultivation containers housed in the container are arranged in horizontal rows, and A predetermined amount is set for each horizontal row unit of cultivation containers, each of which is opened by a lid opening / closing mechanism arranged in a plurality of rows at predetermined intervals as a set, and the lids are opened by the plurality of rows of lid opening / closing mechanisms. Liquid inoculum is supplied and inoculated via liquid inoculum supply mechanisms arranged in multiple rows in the cultivation container, and after inoculation, a plurality of rows of cultivation containers in a horizontal row unit with open lids after inoculation The lids are closed by the lid opening / closing mechanisms arranged in a plurality of rows at the mouth of the cultivation container, and the filling and inoculation efficiency can be increased. Nire The opening and closing mechanism of the lid is opened and closed by the lid opening and closing mechanism for the cultivation containers arranged in a plurality of rows at predetermined intervals with other horizontal row units not inoculated as a set. The inoculation operation and the closing operation of the lid by the lid opening and closing mechanism are performed by a series of repetitive operations, so that the cultivation containers stored in the container are inoculated successively in multiple rows of cultivation containers in one row unit. After the inoculation step is completed, the cultivation container in the container is transferred to the unloading side by the transfer mechanism.

In the first aspect of the present invention, in the second aspect of the present invention, a lid opening / closing mechanism that opens and closes the lid of the cultivation container in a single horizontal row is provided in two rows at a predetermined interval. In addition to disposing the liquid inoculum supply mechanism in two rows in accordance with the lid opening and closing mechanism, the lids are simultaneously opened in two rows in a horizontal row unit, and the liquid After filling and inoculating the liquid inoculum by the inoculum supply mechanism, the lid can be closed with respect to the two rows of cultivation containers opened by the lid opening and closing mechanism, thereby minimizing invasion efficiency while minimizing invasion of various bacteria. it can.

Further, in the second aspect of the present invention, the invention according to the third aspect, wherein the lids of the cultivation containers arranged in a unit of a horizontal row of a front row intermittently transferred by the operation of the transport mechanism, The lids of the cultivation containers aligned in the second row are opened and closed by the lid opening and closing mechanism arranged in two rows with the lids of the cultivation containers aligned in the eyes, and then the cultivation containers aligned in the second row of the cultivation containers intermittently transferred by the transport mechanism. The lid and the lids of the cultivation containers arranged in the fourth row are opened and closed by the lid opening and closing mechanism arranged in two rows, so that it is suitable for intermittent transfer of the transport mechanism. In addition, there is an effect that the liquid inoculum can be efficiently inoculated into the cultivation container in the container.

In the second aspect of the present invention, in the invention according to the fourth aspect, the number of columns of the cultivation containers housed in the container is an even number N, and the lid of the cultivation container positioned at the front row is closed. Based on the installation position of the first lid opening and closing mechanism that opens and closes in horizontal rows, the positional relationship of the second lid opening and closing mechanism that opens and closes the cultivation vessel lid in horizontal rows,

Even if the number of cultivation containers accommodated in the container changes, the installation position of the lid opening / closing mechanism can be set to the number of cultivation containers. By setting them together, the cultivation containers can be automatically inoculated in two rows at predetermined intervals in horizontal rows while the cultivation containers in the container are intermittently transferred.

In Claims 1 to 4, in the invention according to Claim 5, the opening operation of the lid opening / closing mechanism and the movement of the liquid seed supply mechanism are moved onto the mouth of the cultivation container. And the closing operation of the lid opening and closing mechanism and the moving operation of detaching the liquid inoculum supply mechanism from the mouth of the cultivation container are operated in synchronization with each other. However, it is possible to improve the efficiency of inoculation of the liquid inoculum, and to minimize the opening time of the lid of the cultivation container to suppress invasion of various bacteria.

Further, in claim 1 to claim 5, in the invention of claim 6, according to the invention of claim 6, the lid opening / closing mechanism is set to the lid closing position of the cultivation container according to the lid opening / closing operation of the lid opening / closing mechanism. A lid opening / closing drive mechanism for reciprocating between a lid open position and a liquid reciprocating mechanism for reciprocating the liquid inoculum supply mechanism between a position separated from an opening position of the cultivation container and a position on the opening of the cultivation container. By also using the inoculum supply drive mechanism, a series of operations such as opening and closing of the lid and inoculation of liquid inoculum can be performed stably.

Further, in Claims 1 to 6, in the invention according to Claim 7, in order to supply the liquid inoculum to the liquid inoculum supply mechanism, a seed inoculated by storing the liquid inoculum is provided. With the storage container, liquid inoculum can be supplied sequentially from the inoculum storage container, reducing the number of cumbersome operations of replenishing and replacing inoculum. This has the effect of increasing the efficiency of inoculation work. BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 is a schematic diagram of the entire configuration of a liquid inoculum inoculation apparatus showing a first embodiment of the present invention, and FIG. 2 is a front view showing a main part of the liquid inoculum inoculation apparatus of FIG. Fig. 3 is a front view of the main part showing the operation state of the lid opening / closing mechanism and the liquid seed supply mechanism of the liquid seed inoculation device of Fig. 2, and Fig. 4 is a view of the liquid seed supply mechanism of Fig. 3. Fig. 5 is a front view of a main part showing a state in which the spray nozzle is moved downward toward the culture vessel side.Fig. 5 shows a state in which the liquid inoculum is supplied from the spray nozzle after the liquid seed supply mechanism is moved toward the culture vessel side. FIG. 6 is a front view of a main part showing a state in which the lid is closed, FIG. 6 is a side view showing a main part of the liquid inoculum supply mechanism when the lid is closed corresponding to FIG. 2, and FIG. FIG. 8 is a side view showing the main part of the liquid seed supply mechanism when the lid is opened, corresponding to FIG. Fig. 9 is a side view of a main part showing a state in which the injection nozzle of the seed-type supply mechanism is moved toward the cultivation container side, and Fig. 9 is a liquid seed germ by the injection nozzle of the liquid-type seed supply apparatus corresponding to Fig. 5. FIG. 10 is a side view showing a main part of a lid opening / closing mechanism corresponding to FIG. 2, and FIG. 11 is a side view showing a main part of the lid opening / closing mechanism shown in FIG. Fig. 12 is a side view showing an operation state (lid-clamped state) of the movable clamping member in the opening / closing mechanism. Fig. 12 is a main part showing a state in which the lid of the cultivation container is removed from Fig. 11 lid-clamped state. Fig. 13 is a schematic view of the inoculation process, showing that the liquid inoculum is inoculated into the cultivation container in the front row and the cultivation vessel in the third row, respectively, based on the cultivation vessel in the front row. Fig. 14 shows the cultivation vessels in the second and fourth rows based on the cultivation vessels in the second row. Fig. 15 is a schematic diagram of the inoculation process showing the state of inoculation of the liquid inoculum respectively in Fig. 15. Fig. 15 is a schematic diagram showing the container transportation state after the completion of the inoculation process of all the cultivation containers stored in the container. FIG. 16 is a front view showing a main part of a liquid inoculum inoculation apparatus according to a second embodiment of the present invention, and FIG. 17 is based on a cultivation container in the front row in the second embodiment. FIG. 18 is a schematic view of an inoculation process showing a state in which a liquid inoculum is inoculated in the cultivation container in the front row and the cultivation container in the fourth row, respectively. Inoculate liquid bacterium into the cultivation containers in the second and fifth rows, respectively, based on the cultivation containers in FIG. 19 is a schematic diagram of an inoculation process showing a state in which a third row of cultivation vessels and a sixth row of cultivation vessels are based on the third row of cultivation vessels in the second embodiment. FIG. 20 is a schematic view of an inoculation process showing a state in which a liquid inoculum is inoculated, and FIG. 20 is a schematic view of a container after completion of the inoculation process of all the cultivation containers housed in the container according to the second embodiment. It is the schematic which shows a conveyance state. BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, a first embodiment of the liquid inoculum inoculation apparatus according to the present invention will be described with reference to FIGS. 1 to 15. A transport mechanism 2 consisting of a conveyor is provided over almost the entire length of the machine frame 1 of the liquid inoculum inoculation device main body. The transport mechanism 2 is provided so that it can be started and stopped by operating a switch on the operation panel 3. A plurality of sprocket wheels 4 are respectively supported on both ends of the machine frame 1, and an endless chain 5 is stretched in parallel between the sprocket wheels 4 provided at both ends of the machine frame 1. The rotation force of the motor 6 is transmitted to the sprocket wheel 4 on the source side.

Then, a cultivation container 8 filled with a culture medium C such as sawdust or rice bran and sealed with a lid 7 is transferred, for example, a plastic container 9 containing four columns and four rows, and a conveyor having a conveyor at the start end side. When the transport mechanism 2 is started, the container 9 is transported toward the transfer end side by running the tune 5 when the transport mechanism 2 is started.

The transport mechanism 2 provided in the machine frame 1 transfers a container 9 in which a plurality of cultivation containers 8 are arranged in rows and columns from the loading side to an area for inoculating the inoculum, and in the inoculation area of the inoculum. The cultivation container 8 is configured to be intermittently transferred together with the container 9. In this case, as a means for stopping the container 9 sent via the transport mechanism 2 when the cultivation container 8 reaches the appropriate inoculum inoculation area, in this embodiment, a stopper mechanism provided with a stopper pin 1 OA is used. 10 is provided, and a container positioning mechanism 11 for positioning and holding the cultivation container 8 when opening and closing the lid 7 while adjusting the position of the cultivation container 8 in the container 9 is provided.

Further, a stopper machine of the container 9 for stopping the container 9 at a predetermined position. The interval between the stop pins 1 OA provided in the structure 10 is arranged in two rows in accordance with the interval between the cultivation containers 8 arranged in a column of the cultivation containers 8 stored in the container 9. Each stopper pin 10A of the mechanism 10 is provided so as to be able to move up and down via an air cylinder 1OB which is a driving means, and each stopper pin 1OA is independently directed upward from the transfer surface of the transfer mechanism 2. It is always energized.

The cultivation container 8 positioned and arranged in the inoculum inoculation area by the container positioning mechanism 11 is provided with a lid opening and closing mechanism 12 for opening and closing the lid 7 of the cultivation container 8 at a position above the inoculum inoculation area. In this embodiment, a lid opening / closing mechanism 12 for opening / closing a horizontal row of lids 7 arranged at the front row of a cultivation container 8 containing four columns and four rows in a container 9 is provided. A lid opening / closing mechanism 12 for opening and closing the lid 7 of the cultivation container 8 arranged in the third row based on the front row in the container 9 is arranged in two rows.

Also, by operating the lid opening / closing mechanisms 12 arranged in two rows, the horizontal row unit 藎 7 arranged at the front row position of the cultivation container 8 by the operation of the cultivation container 8 and the horizontal row unit lid 7 arranged in the third row At the time of opening, the culture medium C in the cultivation vessel 8 in the front row and the third row aligned in the front row and the third row in which the lid 7 is opened in the horizontal row aligned in the front row and the third row. The liquid inoculum B, which is arranged in two rows in each row unit, is sprayed through the liquid inoculum 13 through the co-supply mechanism 13 to fill and inoculate the open cultivation container 8 with the liquid inoculum 8. After this inoculation, the lid 7 is closed to the cultivation container 8 by the return operation of the lid opening / closing mechanism 12, and then the container 9 is intermittently transferred by the transport mechanism 2, and the cultivation container 8 in the container 9 is transferred. The lids 7 of the cultivation containers 8 arranged in the second and fourth rows of the cultivation containers 8 are opened by the lid opening / closing mechanisms 12 arranged in the two rows. In addition to the operation, the liquid inoculum 機構 is similarly sprayed by the liquid inoculum supply mechanism 13 arranged in two rows and inoculated into the cultivation container 8 and all the cultivation containers 8 accommodated in the container 9 are stored. After the inoculation of the culture medium C is completed, the transport mechanism 2 transfers the container 9 to the unloading side.

In addition, the liquid inoculum supply mechanisms 13 arranged in two rows for supplying the liquid inoculum of the present invention into the cultivation container 8 include, in each liquid inoculum supply mechanism 13, an upper side through which the cultivation container 8 passes. Located in the same number as the four cultivation containers 8 in the horizontal row A liquid inoculum injection cylinder 15 equipped with an injection nozzle 14 is provided, and a flexible pipe 16 is connected to each of the liquid inoculum injection cylinders 15 and the pipe 16 It is connected and fixed to the inoculum container 17 for accommodating the inoculum B. The inside of the liquid inoculum injection cylinder 15 is opened and closed by a jet (not shown) to supply and shut off the liquid inoculum B described above. A valve 18 is provided, and a tube 18 for supplying air for opening and closing the injection opening / closing valve is attached and fixed to a liquid-type fungus injection cylinder 15 provided with an injection nozzle 14.

In this embodiment, as shown in FIG. 1, in order to supply the liquid inoculum B from the inoculum storage container 17 to the injection nozzle 14 via the pipe 16, a compressor or the like was used. The pressurized gas supply mechanism 19 is configured to apply air pressure to the inoculum storage container 17 through the filter 20, and the liquid provided in the liquid inoculation cylinder 15 (not shown) In order to operate the injection on-off valve for supplying and shutting off the inoculum B, a pressurized gas supply mechanism 19 A consisting of a compressor or the like for supplying air to the injection on-off valve is provided. The tube 18 is connected via a filter 2 OA between 19 A and the liquid seed injection cylinder 15 provided with an injection opening / closing valve, and the filter 2 OA is connected to the liquid seed injection cylinder 15. Supply of pressurized gas It is interposed a solenoid valve D to perform blocking. In this case, each filter 20, 2 OA is provided for purifying the air sent from the pressurized gas supply mechanism 19, 19 A, and various bacteria are contained in the seed storage container 17. It is configured so as not to get into the inside or to prevent various bacteria from entering the inside of the liquid seed injecting cylinder 15.

In addition, in accordance with the number of the cultivation containers 8 in one horizontal unit, the liquid seed injection cylinder 15 having four injection nozzles 14 provided in the liquid seed supply mechanism 13 is provided with a nozzle fixing member 2. The nozzle mounting member 22 is detachably fixed to a plate-shaped nozzle mounting member 22 via the base plate 1, and the nozzle mounting member 22 is detachably mounted and fixed to a support plate 23, and the support plate 23 is an air cylinder. The air cylinder 24 is fixed on a cylinder support plate 25, and is fixed to a lower end of the cylinder support plate 25. 5 A moves horizontally along the guide rod 26 provided on the body frame 1 side. It is arranged to be able to reciprocate.

In the inoculum inoculation area, each of the lid opening / closing mechanisms 12 for opening / closing the lids 7 of the cultivation containers 8 arranged in two rows is described in Japanese Patent Application Laid-Open No. HEI 5-11035 which was previously proposed by the present applicant. 4 As shown in Japanese Patent Publication No. 2 and the like, a pair of fixed clamping members that allow the lids 7 of the culture vessels 8 positioned and arranged in a horizontal row to be clamped from the upper surface side and the lower surface side thereof. A movable clamping member 28 comprising a clamping shaft 27 and a clamping shaft is provided, and the movable clamping member 28 can be moved forward and backward, and a horizontal line is provided between the movable clamping member 28 and the fixed clamping member 27. An air cylinder 29 is provided as a clamping member opening / closing means for clamping and releasing each unit lid. Each of the cylinders 29 is mounted and fixed on a cylinder holding plate 30 provided along the width direction of the machine frame 1 of the inoculation device main body, and an inverted T-shape is attached to an end of the cylinder holding plate 30. The transmission / moving means 31 comprising a link arm member is attached and fixed, and the fixed clamping member 27, the movable clamping member 28 and the air cylinder 29 attached to the cylinder holding plate 30 are the link arm members. The cultivation container 8 is provided so as to be movable between a lid closing position and a lid opening position of the cultivation container 8 via a transmission moving means 3 1. In this case, the link arm member 3 1 Is provided rotatably via a support shaft 32 provided in the machine frame 1. Further, a drive cylinder 33 is provided below the weeding mechanism 2, and the drive cylinder 3 3 The tip of the rod 34 provided on the link arm member is 31 and is connected and fixed to the end of the link arm member 31 and to the cylinder support plate 25 which is reciprocally movable along the guide rod 26. Both ends of the connection port 35 are pivotally connected to and fixed to the piece 25 A side which is fixedly mounted.

In this embodiment, the drive cylinder 33 reciprocates the lid opening and closing mechanism 12 between the lid closing position and the lid opening position of the cultivation container 8 according to the lid opening and closing operation of the lid opening and closing mechanism 12. A lid opening / closing drive means, and a seed supply supply drive means for reciprocating the liquid inoculum supply mechanism 13 between a position where the liquid inoculum supply mechanism 13 is detached from the mouth position of the cultivation container 8 and a position on the mouth of the cultivation container 8. By this, the opening operation of the lid opening / closing mechanism 12 and the liquid seed supply mechanism 13 are moved onto the mouth of the cultivation container 8. And the closing operation of the lid opening / closing mechanism 12 and the moving operation of detaching the liquid inoculum supply mechanism 13 from the mouth position of the cultivation container 8 are operated in synchronization with each other. It is configured so that:

In the first embodiment, in the liquid inoculum inoculation device, a lid opening and closing mechanism 12 for opening and closing the lid 7 of the cultivation container 8 in a horizontal row unit with reference to the substantially central position of the main body of the inoculum inoculation device, The liquid inoculum supply mechanism 13 that injects and inoculates the liquid inoculum B into the cultivation vessel 8 in one row unit is a set, and the two sets of lid opening and closing mechanisms 1 2 and 1 2 are symmetrical with the liquid inoculum supply mechanism 1. 3 and 13 are provided. In other words, in the inoculum inoculation area, the lid opening and closing mechanisms 12 and 12 arranged in two rows and the liquid insemination supply mechanisms 13 and 13 arranged in two rows are provided. In some cases, the lid opening / closing mechanism and the liquid inoculum supply mechanism having the same configuration may be provided in a pair without being provided symmetrically in some cases.

Next, the operation of the liquid inoculum inoculation device in the above-described configuration will be described. First, a container 9 containing a predetermined number of cultivation containers 8 with lids 7 is placed on the transport mechanism 2 and the container 9 is transported. When the container 9 reaches the seed inoculation area, the transport mechanism 2 transports the container 9. The front end side of the container 9 is regulated and stopped by the stopper pin 1 OA provided on the stopper mechanism 10 which moves up and down so as to be able to move up and down on the feeding surface. As shown in the figure, a series of welding processes are activated when detection is performed by a sensor. (See Fig. 2.) In this case, the position of the shoulder of the cultivation container 8 in the container 9 is regulated in advance by the container positioning mechanism 11, or linked to a series of inoculation processes at the time of inoculation. When the lid 7 is opened and closed, the cultivation container 8 is positioned and held.

When the cultivation container 8 is positioned and fixed, the air cylinder 29 operates to shift from the state shown in FIG. 10 to the state shown in FIG. 11, and the cultivation container positioned and arranged in the horizontal row located at the front row. 8 and the lids 7 of the cultivation containers 8 positioned in the third row and arranged in a horizontal row, are pressed from their upper surfaces by the respective movable clamping members 28 and provided on the lower surface. The lid 7 is clamped and held between the fixed clamping member 27 and the movable clamping member 28.

Next, positioning by the container positioning mechanism 11 was performed, and the lid 7 was sandwiched and held. Later, as shown in FIG. 3, with the operation of the drive cylinder 33, the link arm member 31.1 rotates with the movement of the rod 34 from the support shaft 32 as a starting point, and this link arm The lid 7 is rotated to the open position by the lid opening / closing mechanism 12 attached to the member 3 1, and the connection port 3 attached to the end of the link arm member 3 1 is synchronized with this lid opening operation. 5, a piece 25A attached to and fixed to the cylinder support plate 25 moves along a guide rod 26 provided on the main body frame 1 side. Therefore, the four sprays 14 provided on the liquid seed supply mechanism 13 are transferred to the position of the mouth of the cultivation container 8, that is, right above the cultivation container 8 from which the lid 7 is removed. (See Fig. 3, Fig. 7, Fig. 12, etc.)

Subsequently, the operation of the air cylinder 24 causes the injection nozzle 14 to approach or be inserted into the mouth of the cultivation container 8. (See FIGS. 4 and 8.) Then, as shown in FIGS. 5, 9 and 13, when the lid 7 of the cultivation container 8 is opened by the lid opening / closing mechanism 12, the lid Ί The liquid inoculum B is sprayed through the liquid inoculum supply mechanism 13 into the culture medium C of the cultivation vessel 8 in a horizontal row in which the seeds are opened, and the liquid inoculum B is sprayed in the open cultivation vessel 8 in a row unit. Is filled and inoculated. At this time, the internal pressure in the inoculum storage container 17 is increased by applying air pressure to the inoculum storage container 17 by a pressurized gas supply mechanism 19 such as a compressor. The inoculum B is pushed out to the pipe 16 on the side of the injection nozzle 14, and when the inoculum B injects, that is, not shown, opens and closes the injection opening / closing valve provided inside the injection nozzle 4. For this purpose, air is supplied to the tube 18 by the pressurized gas supply mechanism 19 して and the injection opening / closing valve is opened, whereby the liquid bacteria B is injected from the injection nozzle 14.

After the liquid inoculum B is filled with a predetermined amount by the injection nozzle 14, the injection nozzle 14 is separated from the mouth position of the cultivation container 8 by the return operation of the air cylinder 24, and thereafter the driving cylinder 33 When the injection nozzle 14 retreats and returns from the position of the cultivation container 8 by the return operation of the rod 3 4, the horizontal position where the liquid inoculum B has been inoculated by the rotation return operation of the lid opening and closing mechanism 12. The lid 7 of the row of cultivation containers 8 is fitted.

Therefore, by sequentially operating a series of steps by the opening and closing mechanism 12 and the liquid inoculum supply mechanism 13, the foremost position in the inoculum inoculation area with respect to the transfer direction of the container 9 is The cultivation container 8 in the row and the third cultivation container 8 (the J-th cultivation container 8) can be inoculated in units of one row each.

Then, when a series of inoculation steps based on the cultivation container 8 in the front row was completed, the stopper pin 1 OA that stopped and held the front end of the container 9 once dropped downward via the air cylinder 10 B. Later, the container 9 is returned upward and the transport mechanism 2 is operated, so that the container 9 is transferred in the unloading direction. Due to the transfer operation of the container 9, the stop pin 10 A, which initially stopped and held the front end of the container 9, also tried to return upward, but was stopped and held until now by the transfer operation of the container 9. The upper end of the stopper pin 1 OA abuts against the bottom of the container 9. However, since the upward biasing force of the stopper pin 1OA is set to be weak, the container 9 can be transported smoothly without hindering the transport, and the next row of the stopper mechanism 10 The front end side of the container 9 is stopped and held by another stopper pin 10 A provided in the container 9.

Also, when the container 9 is stopped, a series of inoculation processes based on the second row of the cultivation container 8 are performed by the lid opening / closing mechanism 12 and the liquid inoculum inoculation mechanism 13 as described above. The cultivation containers 8 in the second row and the cultivation containers 8 in the fourth row can be inoculated as shown in FIG. 14, so that all of the cultivation containers 8 in the container 9 can be inoculated.

When the inoculation process of all the cultivation containers 8 accommodated in the container 9 is completed, as shown in FIG. 15, the stopper pins 1OA of the stopper mechanism 10 are moved downward by the air cylinder 10B. After returning to the upper position, the container 9 containing the inoculated cultivation container 8 is transferred to the unloading side by the transport mechanism 2 and the plant i 咅 container 8 to be newly inoculated is stored. The inoculation operation can be continuously performed by supplying the container 9 from the loading side of the transport mechanism 2.

Therefore, the lid 7 is opened by the lid opening / closing mechanism 12 arranged sequentially in a plurality of rows (two rows in the embodiment) with respect to the cultivation containers 8 in units of one horizontal row, and the liquid seed B is inoculated by the liquid seed supply mechanism 13. The cultivation containers 8 contained in the container 9 can be continuously inoculated in a row by repeating the closing operation of the lid 7 by the operation and the lid opening / closing mechanism 1 2. Inoculation efficiency of B can be improved In addition, since the opening and closing operation and the inoculation operation of the lid 7 of the cultivation container 8 are performed in one horizontal row, the opening time of the lid 7 of the cultivation container 8 can be reduced as much as possible, and the invasion of various bacteria can be suppressed. Is also possible.

FIGS. 16 to 20 show a second embodiment of the present invention. The basic configuration of the first embodiment is almost the same as that of the first embodiment described above. The container 9 containing a plurality of cultivation containers 8 arranged in rows and columns is transferred from the loading side to the area where the inoculum is to be inoculated, and the cultivation containers 8 are together with the container 9 in the inoculation area of the inoculum. Is transported intermittently by the transport mechanism 2. In this case, the cultivation containers 8 are arranged and stored in the container 9 in six columns and four rows, and when the cultivation containers 8 reach an appropriate inoculum inoculation area, they are transferred via the transport mechanism 2. A stop mechanism 10 for stopping the container 9 to be sent is provided, and the stop mechanism 10 is provided with stop pins 10 OA arranged in three rows in the transport direction.

The interval between the stoppins 1 OA arranged in three rows is arranged in accordance with the interval between the cultivation containers 8 arranged in a column of the cultivation containers 8 accommodated in the container 9. The pins 1 OA are provided so as to be constantly and independently urged toward the upper side of the transport mechanism 2, and are also provided via an air cylinder 1 OB which is a driving means as in the first embodiment described above. It can be moved up and down. '

Further, in the second embodiment, a lid opening / closing mechanism for opening and closing a horizontal row of lids 7 arranged in the front row of a cultivation container 8 containing six columns and four rows in a container 9 is also provided. And a lid opening / closing mechanism 12 that opens and closes the lid 7 of the cultivation container 8 arranged in the fourth row based on the front row in the container 9, is arranged in two rows. Inoculation by spraying liquid inoculum B to the position of the front row of the cultivation container 8 and the position of the fourth row based on the front row in the container 9 according to the lid opening and closing mechanisms 12 arranged in two rows The inoculum supply mechanism is configured to be arranged in two rows.

In the second embodiment, in the liquid inoculum inoculation apparatus, a lid opening / closing mechanism 12 that opens and closes the lid 7 of the cultivation container 8 in one horizontal row, and the liquid inoculum B is injected into the cultivation container 8 in one horizontal row. The same type of lid opening and closing machine as a set of the liquid inoculum supply mechanism 13 to be inoculated Structure 12 and liquid inoculum supply mechanism 13 are installed in duplicate.

In the second embodiment having the above-described configuration, the lids 7 are arranged in a row and arranged in the front row of the cultivation container 8 by the operation of the lid opening and closing mechanisms 12 arranged in two rows. At the time of opening with the lid 7 of the horizontal row unit aligned in the front row, the front row and the fourth row aligned with the system IJ As shown in Fig. 17, the culture medium C in the culture vessel 8 is supplied through the injection nozzle 14 of the liquid seed supply mechanism 13 in which the liquid seed bacteria B are arranged in two rows for each row. Cultivation in the cultivation container 8 by filling and inoculating the opened cultivation container 8 with the liquid inoculum B, and inoculating the lid 7 by the return operation of the lid opening / closing mechanism 12 after this inoculation. A series of inoculation processes based on container 8 is completed.

Then, when a series of inoculation steps based on the cultivation container 8 in the front row was completed, the stopper pin 1 OA that stopped and held the front end of the container 9 once dropped downward via the air cylinder 10 B. Later, the container 9 is intermittently transferred by the transport mechanism 2 by returning upward and the transport mechanism 2 being operated intermittently. At this time, the stopper pin 1 OA, which stopped and held the front end of the container 9 in the first inoculation step, returns upward, and the stopper pin 1 OA comes into contact with the bottom surface of the container 9, but the stopper pin 1 OA Since the upward biasing force is set to be weak, it can be intermittently transported without hindering the transport of the container 9, and the other stop pins 1 disposed in the next row of the stopper mechanism 10 The front end of the container 9 is stopped and held by the OA (stopper pin 1 OA located at the center). (See Fig. 18)

When the container 9 is stopped, the lids 7 of the cultivation containers 8 arranged in the second and fifth rows of the cultivation containers 8 in the container 9 are respectively arranged in two rows. As shown in Fig. 18, the liquid inoculum B is moved to the second row and the second row by the injection nozzles 14 of the liquid inoculum supply mechanism 13 arranged in two rows in the same manner as shown in Fig. 18. By spraying and inoculating into the cultivation containers 8 arranged in the fifth row, and then closing the lid 7 by the return operation of the lid opening / closing mechanism 12, the cultivation vessels 8 in the second row are used as a reference. A series of inoculation steps is completed. '

When a series of inoculation processes based on the cultivation containers 8 in the second row is completed, the stoma pin 1OA, which stopped and held the front end of the container 9, was once similarly lowered downward. After returning, the container 9 is returned upward, and the transport mechanism 2 is operated, whereby the container 9 is intermittently transported by the transport mechanism 2. At this time, the stopper pin 1 OA located on the front side and the center side comes into contact with the bottom surface of the container 9 when returning upward, but the upward biasing force of the stopper pin 1 OA is weak. Because of this setting, it is possible to intermittently transport the container 9 without hindering the transport of the container 9 itself.By this transport, the front end side of the container 9 is stopped and held by the stop pin 1 OA arranged in the front row Is done. (See Fig. 19)

In addition, when the container 9 is stopped by the stop pin 1 OA located in the front row in the transport direction, the cultivation aligned in the third column of the cultivation container 8 in the container 9 and the sixth column in the last row. The lids 7 of the containers 8 are opened and closed by the respective lid opening / closing mechanisms 12 arranged in two rows, and as shown in FIG. 19, the respective liquid seed supply mechanisms similarly arranged in two rows. 13 Inject the liquid inoculum B into the cultivation containers 8 arranged in the third and sixth columns by using the injection nozzles 14 and inoculate them, then inoculate the lid 7 and then open and close the lid 7 1 2 As a result, a series of inoculation processes based on the third row of cultivation vessels 8 is completed, and as a result, all cultivation vessels 8 contained in the container 9 are closed. The inoculation work process for culture medium C is completed.

When the inoculation process is completed for the culture medium C of all the cultivation containers 8 contained in the container 9, the stopper pin 10A is lowered and the container 9 is moved to the unloading side by the operation of the transport mechanism 2. In addition, by supplying a container 9 containing a cultivation container 8 to be newly inoculated from the loading side of the transport mechanism 2, the inoculation operation can be continuously performed. (See Fig. 20)

Therefore, also in the second embodiment, similarly to the first embodiment, the lids by the lid opening and closing devices 12 arranged in a plurality of rows (two rows in the embodiment) are sequentially arranged for the cultivation containers 8 in one horizontal row. The cultivation containers 8 stored in the container 9 based on the repetition of the opening operation of 7, the inoculation operation of the liquid inoculum B by the liquid inoculum supply mechanism 13 and the closing operation of the lid 7 by the lid opening and closing mechanism 1 2 The inoculation operation of the liquid inoculum B can be improved, and the opening and closing operation of the lid 7 of the cultivation container 8 and the inoculation operation can be performed in one horizontal row. The lid 7 of the cultivation container 8 The release time can be reduced as much as possible, and the invasion of various bacteria can be suppressed. In the above-described embodiment, the number of cultivation containers 8 to be stored in the container 9 is set to four columns and four rows in the first embodiment, and is set to six columns and four rows in the second embodiment. The number of pieces stored in the container may differ due to differences in the size and shape of the cultivation container and lid, and the size and shape of the container, as described in the examples. As described above, in the lid opening / closing mechanism 12 arranged in two rows in one horizontal row, an even number N in the column is desirable as the number of pieces accommodated in the container 9 in the liquid inoculum inoculation apparatus according to the present invention.

In other words, in the two-row lid opening / closing mechanism 12 or the liquid inoculum supply mechanism 13, the inoculation work is always performed in even-numbered rows, so the number of columns arranged for inoculation is switched by matching the even number N If the number of cultivation containers 8 is odd, the injection operation of the liquid inoculum supply mechanism 13 that operates on the rearmost side during the final process in the container 9 is possible. It can be covered by stopping only

Further, as in the above-described embodiments, in the lid opening / closing mechanism 12 arranged in two rows, the number of columns of the cultivation containers 8 stored in the container 9 is an even number N, and the cultivation containers 8 are arranged in the front row. The second lid that opens and closes the lid 7 of the cultivation container 8 in units of horizontal rows based on the installation position of the first lid opening and closing mechanism 12 that opens and closes the lid 7 of the cultivation container 8 in units of horizontal rows By setting the positional relationship of the opening / closing mechanism 12 to the position of the column of (N / 2) +1, even if the number of columns of the cultivation containers 8 accommodated in the container 9 is changed, the lid is not changed. When the installation position of the opening / closing mechanism 12 is the number of columns of the cultivation containers 8, for example, N = 8, the installation position of the second lid opening / closing mechanism 12 is (8/2) + 1 = 5, that is, By setting the cultivation container 8 corresponding to the fifth row, the cultivation container 8 in the container 9 is intermittently transferred while the cultivation container 8 is being moved horizontally. It can automatically perform two rows Dzu' inoculation operations at predetermined intervals in the unit.

It should be noted that the present invention is not limited to the above-described embodiment, but various modifications can be made within the scope of the present invention. As a means of intermittently setting transfer and stop while sending out the container 9 by the transport mechanism 2 to inoculate based on 8 Although the container 9 was stopped by the stop mechanism 10 that stops at the end, the operation of the transport mechanism 2 was performed only by controlling the rotation of the motor without using the stopper mechanism 10. The stop position may be set to be determined.

In the embodiment, as a detecting means for stopping the cultivation container 8 at a predetermined position in the inoculum inoculation area, in the embodiment, the position of the front end of the container 9 is read by the detection sensor to control the operation of the transport mechanism 2. A detection sensor is arranged in relation to the position where the cultivation container 8 is conveyed.The detection sensor detects the cultivation container 8, and when the cultivation container 8 reaches the predetermined inoculum inoculation area based on the detection signal. Alternatively, the transfer of the transfer mechanism 2 may be stopped.

In addition, a container positioning mechanism 11 is provided which can position and hold the cultivation container 8 when the lid 7 is opened and closed while adjusting the position of the cultivation container 8 in the container 9, and the cultivation container 8 is used as the positioning means. It may be a rail-shaped holding member that presses and holds the shoulder of the cultivation container 8 at least when the lid 7 is opened and closed when the arm 7 is opened and closed. What is necessary is that the regulation is maintained.

Further, as the lid opening / closing mechanism 12 in the embodiment, the lid 27 of the culture vessel 8 positioned and arranged in a horizontal line is a pair of fixed holding portions 27 ′ and a pair capable of holding the lid 7 from the upper and lower surfaces thereof. A movable clamping member 28 comprising a mounting shaft is provided, and the movable clamping member 28 is advanced and retreated, and each of the lids 7 in a horizontal row unit is clamped between the movable clamping member 28 and the fixed clamping member 27. An air cylinder 29 was provided to release and release the lid, but a lid opening / closing mechanism consisting of a pinching claw was applied, and a lid vertical movement means was provided to detach the lid 7 from the cultivation container 8. Alternatively, the lid 7 detached by the operation of the lid vertically moving means may be reciprocated between the lid closed position and the lid open position of the cultivation container 8. In addition, when the liquid inoculum supply mechanism 13 is reciprocated between a position where the liquid seed bacteria supply mechanism 13 is detached from the position of the mouth of the cultivation container 8 and a position above the mouth of the cultivation container, in the embodiment, the lid opening and closing mechanism 12 is operated. The injection nozzle 14 provided in the liquid inoculum supply mechanism 13 is arranged so as to be able to move back and forth in the horizontal direction along the guide rod 26 by the operation of the drive cylinder 33 so as not to hinder the operation. From the position directly above cultivation container 8 to cultivation container 8 Although the air cylinder 24 is provided so as to be able to reciprocate up and down so as to approach and separate from each other, in some cases, only the structure that reciprocates the liquid inoculum supply mechanism 13 horizontally may be adopted. The liquid inoculum supply mechanism 13 is moved up and down just above the cultivation container 8 in accordance with the positional relationship of the inoculum inoculation area in the horizontal row unit of the cultivation container 8 accommodated in the container 9. A structure may be adopted, and in that case, it is only necessary to set so as not to hinder the operation of the lid opening / closing mechanism 12.

Also, in each of the above-described embodiments, for maintenance, inspection, or sterilization treatment, the injection nozzle 14, the pipe 16, the tube 18, etc. on the side supplying the liquid inoculum B are replaced or cleaned. Since it is necessary (if necessary) to perform work and disinfection work, the system is configured so that it can be easily and manually replaced by fixing means such as a detachable knob.

In addition, by setting the setting position of the firing nozzle 14 so as to be adjustable according to the height dimension and size of the cultivation container 8 and the positional relationship of the cultivation container 8, the cultivation container 8 The liquid inoculum B can be sprayed well on the inoculation. When the liquid inoculum B in the inoculum storage container 17 runs out, the inoculum storage container 1 is switched to another inoculum storage container 17 filled with liquid inoculum B by switching tubes and pipes. Replacement of 7 can be performed satisfactorily.In some cases, a plurality of inoculum storage containers 17 are prepared, and when liquid inoculation B in the inoculum storage container 17 is exhausted, the valve can be easily switched by switching. It is also possible to do.

In each of the above-described embodiments, the lid opening / closing mechanism 12 includes a lid opening / closing drive unit that reciprocates between the lid closed position and the lid open position of the cultivation container 8, and the liquid seed supply mechanism 13 includes the cultivation container 8. And the drive cylinder 33 also serves as a liquid seed supply drive means for moving back and forth between the position separated from the mouth position of the cultivation container 8 and the position on the mouth of the cultivation container 8. The means may be provided individually, and may be operated by using a driving means such as a cam mechanism or a motor as the driving means.

As described in detail above, according to the present invention, a container containing a plurality of cultivation containers arranged in rows and columns is supplied from the loading side, and the cultivation containers are intermittently transferred to the unloading side. A transport mechanism for transporting the container, and a lid for the cultivation container in the container intermittently transported by the transport mechanism, which is opened and closed in a horizontal row unit, and at a predetermined interval with the horizontal row unit as a set. A lid opening / closing mechanism arranged in a plurality of rows, and a predetermined amount of liquid inoculum is supplied into the cultivation container to each cultivation vessel of each horizontal row whose lid is opened by the lid opening / closing mechanism of the plurality of rows. A liquid inoculum inoculation apparatus characterized by comprising a liquid inoculum supply mechanism arranged in a plurality of rows, wherein the lids are opened by a multi-row lid opening / closing mechanism with a set of cultivation vessels arranged in a row. A predetermined amount of liquid inoculum is supplied to each of the cultivation containers arranged in units of one horizontal row through the liquid inoculum supply mechanism arranged in a plurality of rows in the cultivation container and inoculated, and after the inoculation, the lid is closed. Open Since the shroud is closed by the lid opening and closing mechanisms arranged in a plurality of rows at the mouth of the cultivation container, the efficiency of filling and inoculating the liquid inoculum can be improved, and the opening time of the lid can be increased. By minimizing the length, it is possible to suppress the invasion of various bacteria.In addition, other horizontal units that are not inoculated due to the intermittent transfer of the transport mechanism are arranged as a set and arranged in multiple rows at predetermined intervals. The lid is opened and closed by the lid opening / closing mechanism, the liquid inoculum is inoculated by the liquid seed supply mechanism, and the lid is closed by the lid opening / closing mechanism by a series of repeated operations. The stored cultivation containers are capable of continuously inoculating a plurality of rows of cultivation containers in a set of one horizontal unit, and after the completion of the inoculation step, the transport mechanism. , The cultivation container in the container can be transferred to the unloading side satisfactorily.

In addition, the lid opening / closing mechanism that opens and closes the cultivation container lid in horizontal row units is arranged in two rows at a predetermined interval, and the liquid seed supply mechanism is arranged in two rows in accordance with this lid opening / closing mechanism. As a result, the lids were simultaneously opened in two rows in units of one horizontal row, the liquid inoculum was supplied and inoculated by the liquid inoculum supply mechanism arranged in two rows, and then opened by the lid opening and closing mechanism. The lid can be closed for the two rows of cultivation containers, which can increase the inoculation efficiency and minimize the invasion of various bacteria. At this time, two rows of cultivation vessel lids arranged in a row in the front row and intermittently transported by the operation of the transport mechanism and cultivation vessel lids arranged in the third row were arranged in two rows. Opening / closing operation is performed by the lid opening / closing mechanism, and then the plants arranged in the second row of the cultivation container intermittently transferred by the transport mechanism are arranged. The lid of the culture vessel and the lid of the culture vessel arranged in the fourth row are opened and closed by the lid opening / closing mechanism arranged in two rows, so that the inside of the container is synchronized with the intermittent transfer of the transport mechanism. Has the effect that the liquid inoculum can be efficiently inoculated into the cultivation container of the present invention. When the number of cultivation containers to be stored in the container is set to an even number N, the cultivation is performed based on the lid opening and closing mechanism in the first row, which opens and closes the cultivation container lids in horizontal rows. By setting the positional relationship of the lid opening and closing mechanism in the second row, which opens and closes the lids of the containers in horizontal rows, to the (NZ 2) + 1 column position, the cultivation vessels contained in the container Even if the number of columns changes, the position of the lid opening / closing mechanism is set in accordance with the number of columns in the cultivation container, so that the cultivation containers can be specified in horizontal rows while intermittently transferring the cultivation containers in the container. There is an effect that the seeding work can be automatically performed in two rows at intervals of.

In addition, the liquid inoculum supply mechanism moves in synchronization with the opening of the cultivation container along with the opening operation of the lid by the lid opening and closing mechanism, and then the liquid inoculum supply mechanism supplies the liquid inoculum to the cultivation container, and then opens and closes the lid. The inoculation work efficiency of the liquid inoculum can be improved by synchronizing the closing operation of the lid by the mechanism and the moving operation of detaching the liquid inoculum supply mechanism from the mouth position of the cultivation container, thereby increasing the efficiency of inoculation of the liquid inoculum. The opening time of the lid can be shortened as much as possible to suppress the invasion of various bacteria.

In addition, a lid opening / closing drive mechanism for reciprocating the lid opening / closing mechanism between the lid closing position and the lid opening position of the cultivation container in accordance with the lid opening / closing operation of the lid opening / closing mechanism, and a liquid inoculum supply mechanism for the mouth position of the cultivation container. A series of operations such as opening and closing of the lid and inoculation of liquid inoculum can be stably performed by the inoculum supply drive mechanism that reciprocates between the position where the inoculum is removed from the position and the position on the mouth of the cultivation container. This has the effect.

In addition, in order to supply the liquid inoculum to the liquid inoculum supply mechanism, the inoculum storage container storing the liquid inoculum is provided, so that the liquid inoculum can be sequentially supplied from the inoculum storage container. The number of cumbersome operations of replenishment and replacement can be reduced as compared with the conventional method, so that the inoculation can be performed while the supply of the inoculum can be stabilized and the efficiency of the inoculation can be increased. Industrial applicability The present invention relates to an inoculum inoculation device in which cultivation vessels are arranged vertically and horizontally in a container, and a culture medium inside the cultivation vessels arranged vertically and horizontally is inoculated with a fungus such as mushrooms. A liquid inoculum is used as the inoculum for mushrooms such as, eryngii, etc., and a plurality of rows can be inoculated simultaneously as a set of horizontal rows in the container with cultivation containers arranged vertically and horizontally in the container. A liquid that can stably fill the inoculum while maintaining the supply amount of the liquid inoculum to be supplied into the cultivation container almost constant while improving the inoculation work efficiency by configuring the inoculum in combination with An inoculum inoculation device can be provided.

Claims

The scope of the claims

1. A transport mechanism that supplies containers containing a plurality of cultivation containers arranged in rows and columns from the loading side, and transports the containers to the unloading side while intermittently transporting the cultivation containers, and the transport mechanism intermittently transfers the containers. A lid opening / closing mechanism that opens and closes the lids of the cultivation containers in the container in a horizontal row unit, and is arranged in a plurality of rows at a predetermined interval with the horizontal row unit as a set; A liquid inoculum supply mechanism arranged in a plurality of rows for supplying a predetermined amount of liquid inoculum to each horizontal one-row cultivation container whose lid is opened by the lid opening / closing mechanism, respectively. A liquid inoculum inoculation device, characterized in that:

2. The lid opening / closing mechanism that opens and closes the cultivation container in one horizontal row is arranged in two rows at a predetermined interval, and the liquid seed supply mechanism is arranged in two rows in accordance with the lid opening / closing mechanism. 2. The liquid inoculum inoculation device according to claim 1, wherein the device is provided.

3. The lid in which the lids of the cultivation containers arranged in a row in the front row and intermittently transported by the operation of the transport mechanism and the lids of the cultivation vessels arranged in the third row are arranged in two rows. The opening and closing mechanism is opened and closed by the opening and closing mechanism, and then, the cultivation container lids arranged in the second row of the cultivation vessels intermittently transferred by the transport mechanism and the cultivation vessel lids arranged in the fourth row are arranged in two rows. 3. The liquid inoculum inoculation device according to claim 2, wherein the device is opened and closed by a lid opening and closing mechanism provided.

4. The number of cultivation containers to be stored in the container is an even number N, and the cultivation containers are placed on the basis of the first row of lid opening / closing mechanisms that open and close the cultivation container lids in horizontal rows. The positional relationship of the lid opening / closing mechanism in the second row that opens and closes the lids in units of one horizontal row is set to the column position of (N / 2) +1.

5. The lid opening operation of the lid opening and closing mechanism and the operation of moving the liquid inoculum supply mechanism onto the mouth of the cultivation container operate in synchronization with each other, and the closing operation of the lid opening and closing mechanism and the liquid inoculum supply mechanism. The liquid inoculum according to any one of claims 1 to 4, characterized in that the liquid inoculation is operated in synchronization with a moving operation of detaching the cultivation container from a mouth position of the cultivation container. Inoculation device.

6. A lid opening / closing drive mechanism for reciprocating the lid opening / closing mechanism between the lid closing position and the lid opening position of the cultivation container in accordance with the lid opening / closing operation of the lid opening / closing mechanism, and a liquid inoculum supply mechanism for the mouth of the cultivation container. 6. The inoculum supply drive mechanism for reciprocatingly moving between the position separated from the position and the position on the mouth of the cultivation container, characterized in that it is used in combination with any of claims 1 to 5. Item 2. The liquid inoculum inoculation device according to item 1.

7. The method according to any one of claims 1 to 6, further comprising: a seed container for storing the liquid inoculum for supplying the liquid inoculum to the liquid inoculum supply mechanism. Item 2. The liquid inoculum inoculation device according to item 1.