WO2002048317A3 - Verfahren und vorrichtung zur herstellung von biologischem gewebe in einer wachstumskammer - Google Patents

Verfahren und vorrichtung zur herstellung von biologischem gewebe in einer wachstumskammer Download PDF

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Publication number
WO2002048317A3
WO2002048317A3 PCT/EP2001/014522 EP0114522W WO0248317A3 WO 2002048317 A3 WO2002048317 A3 WO 2002048317A3 EP 0114522 W EP0114522 W EP 0114522W WO 0248317 A3 WO0248317 A3 WO 0248317A3
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WO
WIPO (PCT)
Prior art keywords
growth chamber
stimuli
growth
biological tissue
producing biological
Prior art date
Application number
PCT/EP2001/014522
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English (en)
French (fr)
Other versions
WO2002048317A2 (de
Inventor
Joerg Bauer
Original Assignee
Joerg Bauer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Joerg Bauer filed Critical Joerg Bauer
Priority to US10/450,478 priority Critical patent/US20040096430A1/en
Priority to EA200300662A priority patent/EA005322B1/ru
Priority to EP01270594A priority patent/EP1341900A2/de
Publication of WO2002048317A2 publication Critical patent/WO2002048317A2/de
Publication of WO2002048317A3 publication Critical patent/WO2002048317A3/de

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
    • A61L27/3645—Connective tissue
    • A61L27/365—Bones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/0077—Special surfaces of prostheses, e.g. for improving ingrowth
    • A61F2002/0086—Special surfaces of prostheses, e.g. for improving ingrowth for preferentially controlling or promoting the growth of specific types of cells or tissues
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    • A61F2/02—Prostheses implantable into the body
    • A61F2/28—Bones
    • A61F2002/2817—Bone stimulation by chemical reactions or by osteogenic or biological products for enhancing ossification, e.g. by bone morphogenetic or morphogenic proteins [BMP] or by transforming growth factors [TGF]
    • A—HUMAN NECESSITIES
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    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
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    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
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    • A61F2002/30001—Additional features of subject-matter classified in A61F2/28, A61F2/30 and subgroups thereof
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    • A61F2002/30004—Material related properties of the prosthesis or of a coating on the prosthesis the prosthesis being made from materials having different values of a given property at different locations within the same prosthesis
    • A61F2002/30057—Material related properties of the prosthesis or of a coating on the prosthesis the prosthesis being made from materials having different values of a given property at different locations within the same prosthesis made from both cortical and cancellous adjacent parts
    • A—HUMAN NECESSITIES
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    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
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    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
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    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
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    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
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    • A61F2/46—Special tools or methods for implanting or extracting artificial joints, accessories, bone grafts or substitutes, or particular adaptations therefor
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    • A61F2310/00—Prostheses classified in A61F2/28 or A61F2/30 - A61F2/44 being constructed from or coated with a particular material
    • A61F2310/00005—The prosthesis being constructed from a particular material
    • A61F2310/00179—Ceramics or ceramic-like structures
    • A61F2310/00293—Ceramics or ceramic-like structures containing a phosphorus-containing compound, e.g. apatite
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12N2533/10—Mineral substrates
    • C12N2533/18—Calcium salts, e.g. apatite, Mineral components from bones, teeth, shells
    • C—CHEMISTRY; METALLURGY
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    • C12N2533/40—Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
    • C—CHEMISTRY; METALLURGY
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    • C12N2533/50—Proteins
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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Rheumatology (AREA)
  • Urology & Nephrology (AREA)
  • Vascular Medicine (AREA)
  • Materials For Medical Uses (AREA)
  • Prostheses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Die Erfindung betrifft ein Verfahren und eine Vorrichtung zur Herstellung von Gewebe in einer Wachstumskammer zur Verpflanzung in oder an einen menschlichen oder tierischen Körper. Besonders vorteilhaft ist die Erfindung zur Züchtung von strukturierten funktionellen Knochen einsetzbar. Es werden biologische Zellen auf ein Wachstumsgerüst aufgebracht und beides in einer Wachstumskammer angeordnet. Die strukturierte und funktionelle Züchtung wird durch die gezielte, insbesondere zeitlich oder örtlich verschiedene oder verschiedenartige Einwirkung von biologisch wirksamen Reizen, z.B. mechanischen, elektrischen, magnetischen, chemischen, olfaktorischen, akustischen und/oder optischen Reize erzielt. Es hat sich gezeigt, dass die Ausübung von Reizen, welche denjenigen entsprechen, welchen entsprechendes natürliches Gewebe im Körper natürlicherweise ausgesetzt ist, am geeignetsten ist.
PCT/EP2001/014522 2000-12-12 2001-12-11 Verfahren und vorrichtung zur herstellung von biologischem gewebe in einer wachstumskammer WO2002048317A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/450,478 US20040096430A1 (en) 2000-12-12 2001-12-11 Method and device for producing biological tissue in a growth chamber
EA200300662A EA005322B1 (ru) 2000-12-12 2001-12-11 Способ и устройство для получения биологической ткани в камере роста
EP01270594A EP1341900A2 (de) 2000-12-12 2001-12-11 Verfahren und vorrichtung zur herstellung von biologischem gewebe in einer wachstumskammer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10061704.2 2000-12-12
DE10061704A DE10061704A1 (de) 2000-12-12 2000-12-12 Verfahren und Vorrichtung zur Herstellung von biologischem Gewebe in einer Wachstumskammer

Publications (2)

Publication Number Publication Date
WO2002048317A2 WO2002048317A2 (de) 2002-06-20
WO2002048317A3 true WO2002048317A3 (de) 2002-11-14

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PCT/EP2001/014522 WO2002048317A2 (de) 2000-12-12 2001-12-11 Verfahren und vorrichtung zur herstellung von biologischem gewebe in einer wachstumskammer

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US (1) US20040096430A1 (de)
EP (1) EP1341900A2 (de)
DE (1) DE10061704A1 (de)
EA (1) EA005322B1 (de)
WO (1) WO2002048317A2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10151822B9 (de) * 2001-10-17 2004-10-21 Jürgen Dr. Heubach Vorrichtung und Verfahren zur elektrischen und mechanischen Stimulierung von Zellen und/oder Geweben
DE10349484A1 (de) * 2003-10-21 2005-05-25 Universität Leipzig Verfahren und Bioreaktor zum Kultivieren und Stimulieren von dreidimensionalen, vitalen und mechanisch widerstandsfähigen Zelltransplantaten
US20070026517A1 (en) * 2004-10-19 2007-02-01 Ronny Schulz Method and bioreactor for the cultivation and stimulation of three-dimensional, vitally and mechanically reistant cell transplants
AU2007212110A1 (en) * 2006-02-02 2007-08-16 Innovative Bio Therapies An extracorporeal cell-based therapeutic device and delivery system
US20090081296A1 (en) * 2006-02-02 2009-03-26 Humes H David Extracorporeal cell-based therapeutic device and delivery system
DE102007006843A1 (de) 2007-02-12 2008-08-14 Bioregeneration Gmbh Verfahren und Stützstruktur zum Kultivieren lebender Zellen
AU2009327485A1 (en) * 2008-06-18 2010-06-24 Cytopherx, Inc. Methods for propagation of renal precursor cells
WO2010019643A2 (en) * 2008-08-15 2010-02-18 Innovative Biotherapies, Inc. An extracorporeal cell-based therapeutic device and delivery system
DE102009057698A1 (de) * 2009-12-03 2011-06-09 Martin-Luther-Universität Halle-Wittenberg Zweikammerkultivierungssystem mit elastischer, poröser Membran zur uniaxialen mechanischen Stimulation von Epithelzellen und Epithelzellverbänden für die Testung endogener und exogener Faktoren
US8883210B1 (en) 2010-05-14 2014-11-11 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US9352003B1 (en) 2010-05-14 2016-05-31 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US10130736B1 (en) 2010-05-14 2018-11-20 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
DE102011018365A1 (de) * 2011-04-20 2012-10-25 Bernhard Hildebrandt Biologischer, implantierbarer Gelenkersatz
US8834928B1 (en) 2011-05-16 2014-09-16 Musculoskeletal Transplant Foundation Tissue-derived tissugenic implants, and methods of fabricating and using same
JP6047159B2 (ja) * 2011-08-02 2016-12-21 東京エレクトロン株式会社 電場印加装置を用いて組織を構築するシステム及び方法
DE102012101078A1 (de) * 2012-02-09 2013-10-17 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Stimulationszelle und Verfahren zur in vitro Stimulation von Zellen oder Geweben
CA2986702C (en) 2015-05-21 2023-04-04 David Wang Modified demineralized cortical bone fibers
US10413913B2 (en) 2017-02-15 2019-09-17 Tokyo Electron Limited Methods and systems for dielectrophoresis (DEP) separation

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19500498A1 (de) * 1995-01-10 1996-07-11 Thomas Dr Med Eschenhagen Matrixkörper für die Messung isometrischer Kraftparameter von Zellgewebe, Verfahren zum Kultivieren von Muskelzellen in Kollagengel und zum gleichzeitigen Herstellen von Muskelgewebe enthaltenden Matrixkörpern für die Messung von isometrischen Kraftparametern an diesen Körpern, sowie Vorrichtung zur Durchführung des Verfahrens
EP0798374A1 (de) * 1996-03-01 1997-10-01 Matrix Medical B.V. Verfahren zur in vitro Herstellung von Knochen
WO1999033951A1 (en) * 1997-12-31 1999-07-08 Advanced Tissue Sciences, Inc. Apparatus and method for simulating in vivo conditions while seeding and culturing three-dimensional tissue constructs
DE19808055A1 (de) * 1998-02-27 1999-09-02 Adamietz Verfahren und Apparatur zur Herstellung von dreidimensionalen Gewebezellkulturen
DE19918435A1 (de) * 1998-07-23 2000-01-27 Roehm Gmbh Überzugs- und Bindemittel für orale oder dermale Arzneiformen
DE19842802A1 (de) * 1998-09-18 2000-03-23 Christoph Geissmaier Kultursystem zur optimierten Anregung des Wachstums und der Differenzierung von Knorpelzellen und/oder von Knochenzellen, Verfahren zur Herstellung des Kultursystems sowie Produkt unter Verwendung des Kultursystems
WO2000034442A2 (en) * 1998-12-11 2000-06-15 Advance Tissue Sciences, Inc. Application of shear flow stress to smooth muscle cells for the production of implantable structures

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5902741A (en) * 1986-04-18 1999-05-11 Advanced Tissue Sciences, Inc. Three-dimensional cartilage cultures
US5266480A (en) * 1986-04-18 1993-11-30 Advanced Tissue Sciences, Inc. Three-dimensional skin culture system
US5928945A (en) * 1996-11-20 1999-07-27 Advanced Tissue Sciences, Inc. Application of shear flow stress to chondrocytes or chondrocyte stem cells to produce cartilage
US6333029B1 (en) * 1999-06-30 2001-12-25 Ethicon, Inc. Porous tissue scaffoldings for the repair of regeneration of tissue

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19500498A1 (de) * 1995-01-10 1996-07-11 Thomas Dr Med Eschenhagen Matrixkörper für die Messung isometrischer Kraftparameter von Zellgewebe, Verfahren zum Kultivieren von Muskelzellen in Kollagengel und zum gleichzeitigen Herstellen von Muskelgewebe enthaltenden Matrixkörpern für die Messung von isometrischen Kraftparametern an diesen Körpern, sowie Vorrichtung zur Durchführung des Verfahrens
EP0798374A1 (de) * 1996-03-01 1997-10-01 Matrix Medical B.V. Verfahren zur in vitro Herstellung von Knochen
WO1999033951A1 (en) * 1997-12-31 1999-07-08 Advanced Tissue Sciences, Inc. Apparatus and method for simulating in vivo conditions while seeding and culturing three-dimensional tissue constructs
DE19808055A1 (de) * 1998-02-27 1999-09-02 Adamietz Verfahren und Apparatur zur Herstellung von dreidimensionalen Gewebezellkulturen
DE19918435A1 (de) * 1998-07-23 2000-01-27 Roehm Gmbh Überzugs- und Bindemittel für orale oder dermale Arzneiformen
DE19842802A1 (de) * 1998-09-18 2000-03-23 Christoph Geissmaier Kultursystem zur optimierten Anregung des Wachstums und der Differenzierung von Knorpelzellen und/oder von Knochenzellen, Verfahren zur Herstellung des Kultursystems sowie Produkt unter Verwendung des Kultursystems
WO2000034442A2 (en) * 1998-12-11 2000-06-15 Advance Tissue Sciences, Inc. Application of shear flow stress to smooth muscle cells for the production of implantable structures

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FENG J ET AL: "Promotion of osteogenesis by a piezoelectric biological ceramic", BIOMATERIALS, ELSEVIER SCIENCE PUBLISHERS BV., BARKING, GB, vol. 18, no. 23, 1 December 1997 (1997-12-01), pages 1531 - 1534, XP004098574, ISSN: 0142-9612 *
TANAKA SHIGEO M: "A new mechanical stimulator for cultured bone cells using piezoelectric actuator.", JOURNAL OF BIOMECHANICS, vol. 32, no. 4, April 1999 (1999-04-01), pages 427 - 430, XP002209561, ISSN: 0021-9290 *

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