WO2002044313A1 - Encapsulation de composes dans des vesicules - Google Patents

Encapsulation de composes dans des vesicules Download PDF

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Publication number
WO2002044313A1
WO2002044313A1 PCT/DK2001/000798 DK0100798W WO0244313A1 WO 2002044313 A1 WO2002044313 A1 WO 2002044313A1 DK 0100798 W DK0100798 W DK 0100798W WO 0244313 A1 WO0244313 A1 WO 0244313A1
Authority
WO
WIPO (PCT)
Prior art keywords
vesicle
vesicles
compounds
composition
enzyme
Prior art date
Application number
PCT/DK2001/000798
Other languages
English (en)
Inventor
Thomas Hoenger Callisen
Original Assignee
Novozymes A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes A/S filed Critical Novozymes A/S
Priority to DE60141167T priority Critical patent/DE60141167D1/de
Priority to AU2002220522A priority patent/AU2002220522A1/en
Priority to AT01998143T priority patent/ATE455839T1/de
Priority to EP01998143A priority patent/EP1358308B1/fr
Publication of WO2002044313A1 publication Critical patent/WO2002044313A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D17/00—Detergent materials or soaps characterised by their shape or physical properties
    • C11D17/0039—Coated compositions or coated components in the compositions, (micro)capsules
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38672—Granulated or coated enzymes

Definitions

  • the invention relates to compositions comprising compounds encapsulated in synthetic polymer based vesicles, such as detergent compositions.
  • composition comprising a surfactant and at least one compound encapsulated in a vesicle, wherein the vesicle comprises at least 50% of a synthetic polymer as a vesicle-forming agent.
  • Di- block-co-polymers self-associate as bilayer phases while tri-block-co-polymers, e.g., composed of a hydrophobic central domain connected to hydrophilic domains at each end, form single-molecular smectic layers with a hydrophobic core.
  • Increasing the width of the interior hydrophobic core would typically bring about improved properties with respect to water permeability of the vesicular structure.
  • the stability of the layers is balanced by the choice of subunits, which make up the co-polymers.
  • the average diameter of the polymer-based vesicles are 0.1 ⁇ m to 500 ⁇ m, preferably 0.1 ⁇ m to 100 ⁇ m, more preferably 0.1 ⁇ m to 10 ⁇ m.
  • the vesicles may be uni-lamellar or have an onion-like structure, such as a multi- lamellar structure.
  • Polymer-based vesicles are particularly suitable for use in surfactant solutions, as compared to lipid vesicles (liposomes), due to a better stability towards surfactants and lower water permeability. Polymer-based vesicles are also referred to as polymersomes (in contrast to liposomes).
  • oxidoreductases examples include GluzymeTM (enzyme available from Novozymes A/S).
  • Suitable surfactants for being incorporated in the composition may be non-ionic (including semi-polar), anionic, cationic and/or zwitterionic.
  • the surfactants are preferably anionic or non-ionic.
  • the surfactants are typically present in the composition at a concentration of from 1% to 90% (preferably 5% to 60%, more preferably 10% to 50%) by weight, but the composition may also contain close to 100% surfactant or consist of 100% surfactant.
  • the pH of the composition is pH 4-11 , preferably pH 6- 11 , more preferably in the alkaline range (such as pH 7-10), and particularly pH 8-10, as determined in a 1 % aqueous solution.
  • Suitable cellulases include those of bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Suitable cellulases include cellulases from the genera Bacillus, Pseudomonas, Humicola, Fusarium,
  • the detergent may be a liquid detergent, and the liquid detergent may be aqueous (typically containing up to 70 % water and 0-30 % organic solvent) or non-aqueous.
  • the detergent may contain 0-65 % of a detergent builder or complexing agent such as zeolite, diphosphate, triphosphate, phosphonate, carbonate, citrate, nitrilotriacetic acid, ethylenediaminetetraacetic acid, diethylenetriaminepentaacetic acid, alkyl- or alkenylsuccinic acid, soluble silicates or layered silicates (e.g. SKS-6 from Hoechst).
  • a detergent builder or complexing agent such as zeolite, diphosphate, triphosphate, phosphonate, carbonate, citrate, nitrilotriacetic acid, ethylenediaminetetraacetic acid, diethylenetriaminepentaacetic acid, alkyl- or alkenylsuccinic acid, soluble silicates or layered silicates (e.g. SKS-6 from Hoechst).
  • Chemicals used as buffers and substrates were commercial products of at least reagent grade.
  • the extruded vesicles were places in dialysis tubes (with a molecular cut-off of 100 kDa, product number 235071 from Spectra). Buffer without the substance intended for encapsulation was used as exterior medium.
  • the concentrated vesicle suspensions were analyzed by differential scanning calorimetry to ascertain the properties of the vesicles and the amount of encapsulated enzyme.
  • the encapsulated amylase was also quantified according to a standard alpha-amylase assay (the Phadebas method).
  • the suspensions containing the enriched encapsulated substance, and the control solution are added separately to solutions containing from 0.1 %(w/w) to 10 %(w/w) of various mixtures of linear alkylbenzenesulphonate (Marlon AS3 obtainable from H ⁇ ls) and alcohol ethoxylate (Neodol 25-7 obtainable from Shell Chemicals), ranging from exclusively linear alkylbenzenesulphonate to exclusively alcohol ethoxylate.
  • Bacillus Lentus subtilisin 309 protease (available as Savinase® from Novozymes A/S, Denmark) is added and the samples are incubated for another 24 hours. This is done to degrade

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Detergent Compositions (AREA)
  • Manufacturing Of Micro-Capsules (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

Selon l'invention, des vésicules formées à partir de polymères synthétiques sont utilisées pour encapsuler des composés afin de protéger ces composés de l'environnement chimique dans lequel ils sont utilisés.
PCT/DK2001/000798 2000-12-01 2001-11-30 Encapsulation de composes dans des vesicules WO2002044313A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE60141167T DE60141167D1 (de) 2000-12-01 2001-11-30 Verkapselung von verbindungen in vesikeln
AU2002220522A AU2002220522A1 (en) 2000-12-01 2001-11-30 Encapsulation of compounds in vesicles
AT01998143T ATE455839T1 (de) 2000-12-01 2001-11-30 Verkapselung von verbindungen in vesikeln
EP01998143A EP1358308B1 (fr) 2000-12-01 2001-11-30 Encapsulation de composes dans des vesicules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200001810 2000-12-01
DKPA200001810 2000-12-01

Publications (1)

Publication Number Publication Date
WO2002044313A1 true WO2002044313A1 (fr) 2002-06-06

Family

ID=8159884

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2001/000798 WO2002044313A1 (fr) 2000-12-01 2001-11-30 Encapsulation de composes dans des vesicules

Country Status (5)

Country Link
EP (1) EP1358308B1 (fr)
AT (1) ATE455839T1 (fr)
AU (1) AU2002220522A1 (fr)
DE (1) DE60141167D1 (fr)
WO (1) WO2002044313A1 (fr)

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3576760A (en) * 1969-06-13 1971-04-27 Nat Patent Dev Corp Water soluble entrapping
EP0586303A1 (fr) * 1992-09-01 1994-03-09 L'oreal Composition cosmétique ou pharmaceutique comprenant en association une peroxydase et un agent anti-oxygène singulet
DE4238779A1 (de) * 1992-11-12 1994-05-19 Max Delbrueck Centrum Lyotrophe Mesophasen und ihre Anwendung in der Pharmazie, der Kosmetik und der Dermatologie
US5385959A (en) * 1992-04-29 1995-01-31 Lever Brothers Company, Division Of Conopco, Inc. Capsule which comprises a component subject to degradation and a composite polymer
EP0653485A1 (fr) * 1993-11-12 1995-05-17 Unilever N.V. Composition détergente
EP0724876A2 (fr) * 1995-01-13 1996-08-07 Dow Corning Corporation Vésicules de résine de siloxane MQ et piégeage
WO1997000623A1 (fr) * 1995-06-21 1997-01-09 Capsulis Vehicules de principes actifs a base de tensioactifs non ioniques et leurs applications notamment dans les domaines alimentaire, cosmetique et pharmaceutique
DE19537415A1 (de) * 1995-10-09 1997-04-10 New Standard Gmbh Micro Wirkstoff-Perlen
WO1997024177A1 (fr) * 1995-12-29 1997-07-10 Allied Colloids Limited Particules contenant une enzyme et concentre detergent liquide
WO1998002144A1 (fr) * 1996-07-16 1998-01-22 Capsulis Compositions contenant au moins un acide nucleique
WO1999027907A1 (fr) * 1997-12-01 1999-06-10 Capsulis Procede perfectionne pour eviter la degradation d'un principe actif

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3576760A (en) * 1969-06-13 1971-04-27 Nat Patent Dev Corp Water soluble entrapping
US5385959A (en) * 1992-04-29 1995-01-31 Lever Brothers Company, Division Of Conopco, Inc. Capsule which comprises a component subject to degradation and a composite polymer
EP0586303A1 (fr) * 1992-09-01 1994-03-09 L'oreal Composition cosmétique ou pharmaceutique comprenant en association une peroxydase et un agent anti-oxygène singulet
DE4238779A1 (de) * 1992-11-12 1994-05-19 Max Delbrueck Centrum Lyotrophe Mesophasen und ihre Anwendung in der Pharmazie, der Kosmetik und der Dermatologie
EP0653485A1 (fr) * 1993-11-12 1995-05-17 Unilever N.V. Composition détergente
EP0724876A2 (fr) * 1995-01-13 1996-08-07 Dow Corning Corporation Vésicules de résine de siloxane MQ et piégeage
WO1997000623A1 (fr) * 1995-06-21 1997-01-09 Capsulis Vehicules de principes actifs a base de tensioactifs non ioniques et leurs applications notamment dans les domaines alimentaire, cosmetique et pharmaceutique
DE19537415A1 (de) * 1995-10-09 1997-04-10 New Standard Gmbh Micro Wirkstoff-Perlen
WO1997024177A1 (fr) * 1995-12-29 1997-07-10 Allied Colloids Limited Particules contenant une enzyme et concentre detergent liquide
WO1998002144A1 (fr) * 1996-07-16 1998-01-22 Capsulis Compositions contenant au moins un acide nucleique
WO1999027907A1 (fr) * 1997-12-01 1999-06-10 Capsulis Procede perfectionne pour eviter la degradation d'un principe actif

Also Published As

Publication number Publication date
EP1358308B1 (fr) 2010-01-20
AU2002220522A1 (en) 2002-06-11
DE60141167D1 (de) 2010-03-11
ATE455839T1 (de) 2010-02-15
EP1358308A1 (fr) 2003-11-05

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