WO2002038811A3 - Assay for the detection and quantitation of hemimethylation - Google Patents

Assay for the detection and quantitation of hemimethylation Download PDF

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Publication number
WO2002038811A3
WO2002038811A3 PCT/US2001/047141 US0147141W WO0238811A3 WO 2002038811 A3 WO2002038811 A3 WO 2002038811A3 US 0147141 W US0147141 W US 0147141W WO 0238811 A3 WO0238811 A3 WO 0238811A3
Authority
WO
WIPO (PCT)
Prior art keywords
methylation
cpg dinucleotide
disclosed
novo
rates
Prior art date
Application number
PCT/US2001/047141
Other languages
French (fr)
Other versions
WO2002038811A2 (en
Inventor
Peter A Jones
Gangning Liang
Yashitaka Tomigahara
Peter W Laird
Original Assignee
Univ Southern California
Peter A Jones
Gangning Liang
Yashitaka Tomigahara
Peter W Laird
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Southern California, Peter A Jones, Gangning Liang, Yashitaka Tomigahara, Peter W Laird filed Critical Univ Southern California
Priority to AU2002233988A priority Critical patent/AU2002233988A1/en
Priority to JP2002541125A priority patent/JP2004535757A/en
Priority to CA002428245A priority patent/CA2428245A1/en
Priority to US10/416,075 priority patent/US20040072197A1/en
Priority to EP01984994A priority patent/EP1379685A2/en
Publication of WO2002038811A2 publication Critical patent/WO2002038811A2/en
Publication of WO2002038811A3 publication Critical patent/WO2002038811A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Abstract There is disclosed a method to measure the fraction of DNA molecules that ishemi-methylated at a specific CpG dinucleotide in a particular DNA sequence, in a pool of DNA molecules having mixed DNA methylation states at said CpG dinucleotide. There is also disclosed methods to calculate the rates of de novo-, and maintenance-methylation per cell division that occur at said CpG dinucleotide. Additionally, there is disclosed methylation kits for use in measuring and quantitating hemi-methylation, and for calculating the rates of de novo-, and maintenance-methylation.
PCT/US2001/047141 2000-11-08 2001-11-08 Assay for the detection and quantitation of hemimethylation WO2002038811A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2002233988A AU2002233988A1 (en) 2000-11-08 2001-11-08 Assay for the detection and quantitation of hemimethylation
JP2002541125A JP2004535757A (en) 2000-11-08 2001-11-08 A new assay for detection and quantification of hemimethylation
CA002428245A CA2428245A1 (en) 2000-11-08 2001-11-08 A new assay for the detection and quantitation of hemimethylation
US10/416,075 US20040072197A1 (en) 2001-11-08 2001-11-08 Assay for the detection and quantitation of hemimethylation
EP01984994A EP1379685A2 (en) 2000-11-08 2001-11-08 Assay for the detection and quantitation of hemimethylation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24719100P 2000-11-08 2000-11-08
US60/247,191 2000-11-08

Publications (2)

Publication Number Publication Date
WO2002038811A2 WO2002038811A2 (en) 2002-05-16
WO2002038811A3 true WO2002038811A3 (en) 2003-10-23

Family

ID=22933958

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/047141 WO2002038811A2 (en) 2000-11-08 2001-11-08 Assay for the detection and quantitation of hemimethylation

Country Status (5)

Country Link
EP (1) EP1379685A2 (en)
JP (1) JP2004535757A (en)
AU (1) AU2002233988A1 (en)
CA (1) CA2428245A1 (en)
WO (1) WO2002038811A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060134643A1 (en) 2000-06-19 2006-06-22 Kurt Berlin Bisulfite conversion of DNA
DE10347399B4 (en) * 2003-10-09 2005-09-15 Epigenomics Ag Bisulfite conversion for detection of cytosine methylation in DNA by optimized purification
US6649347B2 (en) * 2000-06-23 2003-11-18 Chromatin, Inc. Use of methylated nucleic acid segments for isolating centromere DNA
DE10346363B4 (en) * 2003-09-30 2005-09-29 Epigenomics Ag Method for methylation analysis of DNA
JP4571643B2 (en) * 2003-10-09 2010-10-27 エピゲノミクス アーゲー DNA conversion by improved bisulfite.
AU2004284456B2 (en) 2003-10-21 2012-04-19 Orion Genomics Llc Methods for quantitative determination of methylation density in a DNA locus
DE102005011398A1 (en) * 2005-03-03 2006-09-14 Epigenomics Ag Method for the investigation of cytosine methylations in DNA

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997046705A1 (en) * 1996-06-03 1997-12-11 The Johns Hopkins University School Of Medicine Methylation specific detection
WO1998056952A1 (en) * 1997-06-09 1998-12-17 University Of Southern California A cancer diagnostic method based upon dna methylation differences

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997046705A1 (en) * 1996-06-03 1997-12-11 The Johns Hopkins University School Of Medicine Methylation specific detection
WO1998056952A1 (en) * 1997-06-09 1998-12-17 University Of Southern California A cancer diagnostic method based upon dna methylation differences

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
MASS M J ET AL: "ARSENIC ALTERS CYTOSINE METHYLATION PATTERNS OF THE PROMOTER OF THETUMOR SUPPRESSOR GENE P53 IN HUMAN LUNG CELLS: A MODEL FOR A MECHANISM OF CARCINOGENESIS", MUTATION RESEARCH, AMSTERDAM, NL, vol. 386, no. 3, 1997, pages 263 - 277, XP001022349, ISSN: 0027-5107 *
SINGER-SAM J ET AL: "A quantitative HpaII-PCR assay to measure methylation of DNA from a small number of cells", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 3, no. 18, 1990, pages 687, XP002080182, ISSN: 0305-1048 *
WOODCOCK D M ET AL: "Asymmetric methylation in the hypermethylated CpG promoter region of the human L1 retrotransposon", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 272, no. 12, 21 March 1997 (1997-03-21), pages 7810 - 7816, XP002228796, ISSN: 0021-9258 *
ZHOU Y ET AL: "AN APPARENT CASE OF NONSYMMETRICAL AND SUSTAINED STRAND-SPECIFIC HEMIMETHYLATION IN DC8 GENE OF CARROT", GENOME, OTTAWA, CA, vol. 41, no. 23, 1998, pages 23 - 33, XP001135029, ISSN: 0831-2796 *

Also Published As

Publication number Publication date
AU2002233988A1 (en) 2002-05-21
CA2428245A1 (en) 2002-05-16
EP1379685A2 (en) 2004-01-14
JP2004535757A (en) 2004-12-02
WO2002038811A2 (en) 2002-05-16

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