WO2001007649A3 - Method for detecting microorganisms - Google Patents

Method for detecting microorganisms Download PDF

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Publication number
WO2001007649A3
WO2001007649A3 PCT/EP2000/006946 EP0006946W WO0107649A3 WO 2001007649 A3 WO2001007649 A3 WO 2001007649A3 EP 0006946 W EP0006946 W EP 0006946W WO 0107649 A3 WO0107649 A3 WO 0107649A3
Authority
WO
WIPO (PCT)
Prior art keywords
detecting microorganisms
microorganisms
detecting
kit
implementing
Prior art date
Application number
PCT/EP2000/006946
Other languages
German (de)
French (fr)
Other versions
WO2001007649A2 (en
Inventor
Jiri Snaidr
Claudia Beimfohr
Original Assignee
Vermicon Ag
Jiri Snaidr
Claudia Beimfohr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vermicon Ag, Jiri Snaidr, Claudia Beimfohr filed Critical Vermicon Ag
Priority to AU68255/00A priority Critical patent/AU6825500A/en
Publication of WO2001007649A2 publication Critical patent/WO2001007649A2/en
Publication of WO2001007649A3 publication Critical patent/WO2001007649A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

Abstract

The invention relates to a method for detecting microorganisms in a sample using a nucleic acid probe and to a kit for implementing said method.
PCT/EP2000/006946 1999-07-22 2000-07-20 Method for detecting microorganisms WO2001007649A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU68255/00A AU6825500A (en) 1999-07-22 2000-07-20 Method for detecting microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19934510A DE19934510B4 (en) 1999-07-22 1999-07-22 Method for the detection of microorganisms
DE19934510.4 1999-07-22

Publications (2)

Publication Number Publication Date
WO2001007649A2 WO2001007649A2 (en) 2001-02-01
WO2001007649A3 true WO2001007649A3 (en) 2001-08-09

Family

ID=7915754

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/006946 WO2001007649A2 (en) 1999-07-22 2000-07-20 Method for detecting microorganisms

Country Status (3)

Country Link
AU (1) AU6825500A (en)
DE (1) DE19934510B4 (en)
WO (1) WO2001007649A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10022304B4 (en) * 2000-05-08 2004-01-15 Vermicon Ag Methods for the identification, quantification and visualization of microorganisms
WO2004042082A1 (en) * 2002-11-06 2004-05-21 Kemira Oyj A method for monitoring the presence of harmful microorganisms in paper industry
DE10344057B3 (en) * 2003-09-23 2005-06-09 Vermicon Ag Method for the specific rapid detection of harmful microorganisms
AT512416B1 (en) 2012-02-13 2013-10-15 Greiner Bio One Gmbh ARRANGEMENT AND METHOD FOR DETECTING MICROORGANISMS IN A CULTURAL VESSEL
CN107089768A (en) * 2017-05-19 2017-08-25 严秋明 A kind of sewage disposal and the method and system for reclaiming water body resource
CN108896728A (en) * 2018-07-20 2018-11-27 马明昌 A kind of Intelligent water quality monitoring system based on new energy unmanned boat platform

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0336412A2 (en) * 1988-04-08 1989-10-11 SCLAVO S.p.A. Synthetic oligonucleotides useful for the determination of Chlamydia trachomatis in a biological sample
US5863767A (en) * 1993-06-29 1999-01-26 Chiron Corporation Truncated keratinocyte growth factor (KGF) having increased biological activity
DE19916610A1 (en) * 1998-05-22 1999-11-25 Creatogen Biosciences Gmbh Detecting antibiotic resistance in microorganisms by in situ characterization of probes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0336412A2 (en) * 1988-04-08 1989-10-11 SCLAVO S.p.A. Synthetic oligonucleotides useful for the determination of Chlamydia trachomatis in a biological sample
US5863767A (en) * 1993-06-29 1999-01-26 Chiron Corporation Truncated keratinocyte growth factor (KGF) having increased biological activity
DE19916610A1 (en) * 1998-05-22 1999-11-25 Creatogen Biosciences Gmbh Detecting antibiotic resistance in microorganisms by in situ characterization of probes

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AMANN R I ET AL: "PHYLOGENETIC IDENTIFICATION AND IN SITU DETECTION OF INDIVIDUAL MICROBIAL CELLS WITHOUT CULTIVATION", MICROBIOLOGICAL REVIEWS,US,AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC, vol. 59, no. 1, 1 March 1995 (1995-03-01), pages 143 - 169, XP002026194, ISSN: 0146-0749 *
BEIMFOHR CLAUDIA ET AL: "In situ identification of lactococci, enterococci and streptococci.", SYSTEMATIC AND APPLIED MICROBIOLOGY, vol. 16, no. 3, 1993, pages 450 - 456, XP000982317, ISSN: 0723-2020 *
BRAUN-HOWLAND ELLEN B ET AL: "Development of a rapid method for detecting bacterial cells in situ using 16S rRNA-targeted probes.", BIOTECHNIQUES, vol. 13, no. 6, 1992, pages 928 - 932, 934, XP002160427, ISSN: 0736-6205 *
DELONG E F ET AL: "PHYLOGENETIC STAINS RIBOSOMAL RNA-BASED PROBES FOR THE IDENTIFICATION OF SINGLE CELLS", SCIENCE (WASHINGTON D C), vol. 243, no. 4896, 1989, pages 1360 - 1363, XP002160429, ISSN: 0036-8075 *
MARKOVIC B ET AL: "Quantitation of Fc-gamma-RII mRNA in platelets and megakaryoblastic cell lines by a new method of in situ hybridization.", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 172, no. 1, 1994, pages 105 - 114, XP002160428, ISSN: 0022-1759 *
SNAIDR J ET AL: "Application of the 16S rRNA approach to industrial sewage treatment plants: Progress towards a complete community analysis.", MEDEDELINGEN FACULTEIT LANDBOUWKUNDIGE EN TOEGEPASTE BIOLOGISCHE WETENSCHAPPEN UNIVERSITEIT GENT, vol. 63, no. 4B, 1998, pages 1741 - 1746, XP000982316 *

Also Published As

Publication number Publication date
AU6825500A (en) 2001-02-13
WO2001007649A2 (en) 2001-02-01
DE19934510A1 (en) 2001-01-25
DE19934510B4 (en) 2009-04-16

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