WO2002034775A3 - Nucleotide sequences encoding the hemd and hemb genes - Google Patents

Nucleotide sequences encoding the hemd and hemb genes Download PDF

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Publication number
WO2002034775A3
WO2002034775A3 PCT/EP2001/011705 EP0111705W WO0234775A3 WO 2002034775 A3 WO2002034775 A3 WO 2002034775A3 EP 0111705 W EP0111705 W EP 0111705W WO 0234775 A3 WO0234775 A3 WO 0234775A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotide
amino acid
acid sequence
hemd
polypeptide
Prior art date
Application number
PCT/EP2001/011705
Other languages
French (fr)
Other versions
WO2002034775A2 (en
Inventor
Mike Farwick
Klaus Huthmacher
Walter Pfefferle
Natalie Schischka
Achim Marx
Original Assignee
Degussa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10145585A external-priority patent/DE10145585A1/en
Application filed by Degussa filed Critical Degussa
Priority to AU2002218223A priority Critical patent/AU2002218223A1/en
Publication of WO2002034775A2 publication Critical patent/WO2002034775A2/en
Publication of WO2002034775A3 publication Critical patent/WO2002034775A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01024—Porphobilinogen synthase (4.2.1.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01075—Uroporphyrinogen-III synthase (4.2.1.75)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to an isolated polynucleotide containing a polynucleotide sequence, selected from the group a) polynucleotide that is at least 70 % identical with a polynucleotide encoding a polypeptide that contains the amino acid sequence of SEQ ID No. 2, b) polynucleotide that is at least 70 % identical with a polynucleotide encoding a polypeptide that contains the amino acid sequence of SEQ ID No. 3, c) polynucleotide encoding a polypeptide that contains an amino acid sequence that is at least 70 % identical with the amino acid sequence of SEQ ID No. 2, d) polynucleotide encoding a polypeptide that contains an amino acid sequence that is at least 70 % identical with the amino acid sequence of SEQ ID No. 3, e) polynucleotide that is complementary to the polynucleotides of a), b), c) and d), and f) polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of a), b), c), d) and e), and a method for the fermentative production of L-amino acids using coryneform bacteria, in which at least the hemD-gene and/or the hemB-gene is/are amplified, and the use, as Hybridization probes, of polynucleotides that contain the sequences according to the invention.
PCT/EP2001/011705 2000-10-28 2001-10-10 Nucleotide sequences encoding the hemd and hemb genes WO2002034775A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002218223A AU2002218223A1 (en) 2000-10-28 2001-10-10 Nucleotide sequences encoding the hemd and hemb genes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10053708 2000-10-28
DE10053708.1 2000-10-28
DE10145585A DE10145585A1 (en) 2000-10-28 2001-09-15 For the genes hemD and hemB coding nucleotide sequences
DE10145585.2 2001-09-15

Publications (2)

Publication Number Publication Date
WO2002034775A2 WO2002034775A2 (en) 2002-05-02
WO2002034775A3 true WO2002034775A3 (en) 2002-09-19

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/011705 WO2002034775A2 (en) 2000-10-28 2001-10-10 Nucleotide sequences encoding the hemd and hemb genes

Country Status (2)

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AU (1) AU2002218223A1 (en)
WO (1) WO2002034775A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110819615B (en) * 2020-01-07 2020-04-07 中国科学院天津工业生物技术研究所 Uroporphyrinogen III synthetase mutant, mutant gene and application of mutant gene in preparation of vitamin B12

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0219027A2 (en) * 1985-10-04 1987-04-22 Kyowa Hakko Kogyo Co., Ltd. Process for producing amino acids
EP0387527A1 (en) * 1989-03-14 1990-09-19 Degussa Aktiengesellschaft Process for the fermentative production of L-lysine
WO2001000843A2 (en) * 1999-06-25 2001-01-04 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding metabolic pathway proteins
EP1108790A2 (en) * 1999-12-16 2001-06-20 Kyowa Hakko Kogyo Co., Ltd. Novel polynucleotides

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0219027A2 (en) * 1985-10-04 1987-04-22 Kyowa Hakko Kogyo Co., Ltd. Process for producing amino acids
EP0387527A1 (en) * 1989-03-14 1990-09-19 Degussa Aktiengesellschaft Process for the fermentative production of L-lysine
WO2001000843A2 (en) * 1999-06-25 2001-01-04 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding metabolic pathway proteins
EP1108790A2 (en) * 1999-12-16 2001-06-20 Kyowa Hakko Kogyo Co., Ltd. Novel polynucleotides

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 12 April 1994 (1994-04-12), XP002199778 *
DATABASE EMBL 15 January 1997 (1997-01-15), XP002193453 *
DATABASE EMBL 17 July 1997 (1997-07-17), XP002199777 *
DATABASE EMBL 17 June 1999 (1999-06-17), XP002199775 *
DATABASE EMBL 22 January 1994 (1994-01-22), XP002193454 *
DATABASE EMBL 30 January 1995 (1995-01-30), XP002199776 *
DATABASE WPI Section Ch Week 200114, Derwent World Patents Index; Class B04, AN 2001-137957, XP002193456 *
KENYON S H ET AL: "EFFECT OF HYDRAZINE UPON VITAMIN B12-DEPENDENT METHIONINE SYNTHASE ACTIVITY AND THE SULPHUR AMINO ACID PATHWAY IN ISOLATED RAT HEPATOCYTES", 1999, BIOCHEMICAL PHARMACOLOGY, VOL. 57, PAGE(S) 1311-1319, XP001062746 *
STAMFORD ET AL: "BIOSYNTHESIS OF VITAMIN B12: THE PREPARATIVE MULTI-ENZYME SYNTHESIS OF PRECORRIN-3A AND 20-METHYLSIROHYDROCHLORIN (A 2,7,20-TRIMETHYLISOBACTERIOCHLORIN)", 1996, BIOCHEM. J. VOL. 313, PAGE(S) 335-342, XP001062747 *
WEISSBACH H ET AL: "REGULATION OF METHIONINE SYNTHESIS IN ESCHERICHIA COLI", MOLECULAR MICROBIOLOGY, BLACKWELL SCIENTIFIC, OXFORD, GB, VOL. 5, NR. 7, PAGE(S) 1593-1597, ISSN: 0950-382X, XP001037155 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110819615B (en) * 2020-01-07 2020-04-07 中国科学院天津工业生物技术研究所 Uroporphyrinogen III synthetase mutant, mutant gene and application of mutant gene in preparation of vitamin B12

Also Published As

Publication number Publication date
AU2002218223A1 (en) 2002-05-06
WO2002034775A2 (en) 2002-05-02

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