WO2002029405A3 - Verfahren zum testen von substanzen mit lytischer wirkung, anordnung zur durchführung und anwendung des verfahrens - Google Patents

Verfahren zum testen von substanzen mit lytischer wirkung, anordnung zur durchführung und anwendung des verfahrens Download PDF

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Publication number
WO2002029405A3
WO2002029405A3 PCT/DE2001/003803 DE0103803W WO0229405A3 WO 2002029405 A3 WO2002029405 A3 WO 2002029405A3 DE 0103803 W DE0103803 W DE 0103803W WO 0229405 A3 WO0229405 A3 WO 0229405A3
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WO
WIPO (PCT)
Prior art keywords
carrying
allows
lytic effect
inventive method
testing substances
Prior art date
Application number
PCT/DE2001/003803
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English (en)
French (fr)
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WO2002029405A2 (de
Inventor
Erik Eschbach
Crawford Linda Medlin
Original Assignee
Stiftung A Wegener Inst Polar
Erik Eschbach
Crawford Linda Medlin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stiftung A Wegener Inst Polar, Erik Eschbach, Crawford Linda Medlin filed Critical Stiftung A Wegener Inst Polar
Priority to AU2002223463A priority Critical patent/AU2002223463A1/en
Publication of WO2002029405A2 publication Critical patent/WO2002029405A2/de
Publication of WO2002029405A3 publication Critical patent/WO2002029405A3/de

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)

Abstract

Das Verfahren (ELA) basiert auf dem Lyse-Detektions-Prinzip, führt aber eine hocheffiziente Parallelverarbeitung der zu testenden Substanz (TS, AP) in νl-kleinen Einzelvolumina unterschiedlicher Ausbildungsformen (CV, SV) ein, die matrizenförmig (MPC, MPV) angeordnet sein können. Dadurch ergibt sich ein hoher Probendurchsatz verbunden mit einer entsprechend hohen Verfahrensökonomie und -sicherheit. In Ausführungsformen arbeitet das erfindungsgemässe Verfahren bei einer Absorptionswellenlänge von 414 nm, wodurch eine gegenüber bekannten Verfahren um ca. das Zehnfache gesteigerte Empfindlichkeit erreicht wird. Das erfindungsgemässe Verfahren lässt sich einfach automatisieren und damit kommerziell in Anwendungen sowohl als Test-, als Frühwarn- oder auch als Screeningsystem umsetzen.
PCT/DE2001/003803 2000-09-30 2001-09-30 Verfahren zum testen von substanzen mit lytischer wirkung, anordnung zur durchführung und anwendung des verfahrens WO2002029405A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002223463A AU2002223463A1 (en) 2000-09-30 2001-09-30 Method for testing substances that have a lytic effect, system for carrying out and using said method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2000149732 DE10049732C2 (de) 2000-09-30 2000-09-30 Verfahren zum Testen von Substanzen mit lytischer Wirkung, Anordnung zur Durchführung und Anwendung des Verfahrens
DE10049732.2 2000-09-30

Publications (2)

Publication Number Publication Date
WO2002029405A2 WO2002029405A2 (de) 2002-04-11
WO2002029405A3 true WO2002029405A3 (de) 2002-09-06

Family

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PCT/DE2001/003803 WO2002029405A2 (de) 2000-09-30 2001-09-30 Verfahren zum testen von substanzen mit lytischer wirkung, anordnung zur durchführung und anwendung des verfahrens

Country Status (3)

Country Link
AU (1) AU2002223463A1 (de)
DE (1) DE10049732C2 (de)
WO (1) WO2002029405A2 (de)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5135850A (en) * 1989-01-27 1992-08-04 Spiral Recherche Et Developpment Process for the determination by means of free radicals of the antioxidant properties of a living organism or a potentially aggressive age
US5955295A (en) * 1992-10-30 1999-09-21 Micro-Med, Inc. Micro lysis-analysis process to measure cell characteristics and diagnose diseases

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3916595A1 (de) * 1989-05-22 1990-11-29 Boehringer Mannheim Gmbh Verfahren zur nichtradioaktiven messung der nucleinsaeuresynthese in eukaryontischen zellen
DE4238497A1 (de) * 1992-11-14 1994-05-19 Slt Labinstruments Gmbh Automatische Erfassung von Haemolyse
DE4338731A1 (de) * 1993-11-12 1995-05-18 Slt Labinstruments Deutschland Methode zur Bestimmung von Festphasen-Immunassays

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5135850A (en) * 1989-01-27 1992-08-04 Spiral Recherche Et Developpment Process for the determination by means of free radicals of the antioxidant properties of a living organism or a potentially aggressive age
US5955295A (en) * 1992-10-30 1999-09-21 Micro-Med, Inc. Micro lysis-analysis process to measure cell characteristics and diagnose diseases

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ARZUL G. ET AL: "A Haemolytic Test to assay Toxins excreted by the Marine Dinoflagellate Gyrodinium cf. Aureolum", WATER RESEARCH, vol. 28, no. 4, 1994, pages 961 - 965, XP002192609 *
COOPER TERRANCE G: "Biochemische Arbeitsmethoden", 1980, WALTER DE GRYTER & CO, BERLIN, NEW YORK, XP002192610 *
ESCHBACH ERIK ET AL: "Improved Erythrocyte Lysis Assay in Microtitre Plates for Senistive Detection and Efficient Measurement of Haemolytic Compounds from Ichthyotoxic Algae", JOURNAL OF APPLIED TOXICOLOGY, November 2001 (2001-11-01), john wiley & sons, pages 513-513 - 519, XP001061951 *
KLERX J P A M ET AL: "Microassay for Colorimetric Estimation of Complement Activity in Guinea Pig, Human and Mouse Serum", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 63, 1983, elsevier, pages 215 - 220, XP001062146 *
NAKAJIMA I ET AL: "Toxicity of Benthic Dinoflagellates in Okinawa Japan", BULLTETIN OF THE JAPANESE SOCIETY OF SCIENTIFIC FISHERIES, vol. 47, no. 8, 1981, Laboratory of Food Hygiene, Faculty of Agriculture, Tohoku Univeristy, Tsutsumi-dori, Sendai 980, pages 1029 - 1034, XP001062261 *
PIKE B L ET AL: "A Simple Semi Automated Plaque Method for the Detection of Antibody Forming Cell Clones in Micro Cultures", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 52, no. 1, 1982, elsevier, pages 25 - 37, XP002192606 *
SIMPSON M A ET AL: "Spectrophotometric Determination of Lymphocyte Mediated Sheep Red Blood Cell Hemolysis In-Vitro", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 21, no. 1-2, 1978, elsevier, pages 159 - 165, XP002192607 *

Also Published As

Publication number Publication date
DE10049732C2 (de) 2003-06-26
AU2002223463A1 (en) 2002-04-15
DE10049732A1 (de) 2002-04-18
WO2002029405A2 (de) 2002-04-11

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