WO2002016652A3 - Dispositif de traitement rapide d'echantillons d'adn, par manipulation, thermocyclage et purification integrees de liquides - Google Patents

Dispositif de traitement rapide d'echantillons d'adn, par manipulation, thermocyclage et purification integrees de liquides Download PDF

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Publication number
WO2002016652A3
WO2002016652A3 PCT/US2001/026611 US0126611W WO0216652A3 WO 2002016652 A3 WO2002016652 A3 WO 2002016652A3 US 0126611 W US0126611 W US 0126611W WO 0216652 A3 WO0216652 A3 WO 0216652A3
Authority
WO
WIPO (PCT)
Prior art keywords
needle
sample chamber
thermocycling
purification
sample processing
Prior art date
Application number
PCT/US2001/026611
Other languages
English (en)
Other versions
WO2002016652A2 (fr
Inventor
James Benn
Jean-Francois Manchec
Original Assignee
Genome Therapeutics Corp
James Benn
Jean-Francois Manchec
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genome Therapeutics Corp, James Benn, Jean-Francois Manchec filed Critical Genome Therapeutics Corp
Priority to AU2001286784A priority Critical patent/AU2001286784A1/en
Publication of WO2002016652A2 publication Critical patent/WO2002016652A2/fr
Publication of WO2002016652A3 publication Critical patent/WO2002016652A3/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4005—Concentrating samples by transferring a selected component through a membrane
    • G01N2001/4016—Concentrating samples by transferring a selected component through a membrane being a selective membrane, e.g. dialysis or osmosis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • General Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

L'invention concerne un système et une unité d'ultrafiltration et de dialyse à plaques horizontales, comportant une chambre d'échantillons, un orifice de positionnement de seringue, pourvu d'un joint capable de constituer un scellement étanche aux fluides après son percement à l'aide d'une aiguille, un arrêt d'aiguille empêchant une aiguille de trop pénétrer dans la chambre d'échantillons, ainsi qu'un guide d'aiguille, du type entonnoir, formé dans l'orifice de positionnement de seringue, de manière à guider une aiguille en direction de la chambre d'échantillons. Cette chambre est également dotée, le long d'une de ses portions, d'une membrane de dialyse ou d'ultrafiltration et elle est couplée de manière fluidique à une extrémité distale de l'orifice de positionnement de seringue et d'un trou d'aération.
PCT/US2001/026611 2000-08-25 2001-08-24 Dispositif de traitement rapide d'echantillons d'adn, par manipulation, thermocyclage et purification integrees de liquides WO2002016652A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001286784A AU2001286784A1 (en) 2000-08-25 2001-08-24 Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US22823900P 2000-08-25 2000-08-25
US60/228,239 2000-08-25
US26603501P 2001-02-02 2001-02-02
US60/266,035 2001-02-02

Publications (2)

Publication Number Publication Date
WO2002016652A2 WO2002016652A2 (fr) 2002-02-28
WO2002016652A3 true WO2002016652A3 (fr) 2003-07-10

Family

ID=26922164

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2001/026555 WO2002015949A2 (fr) 2000-08-25 2001-08-24 Dispositif pour l'identification d'une sequence nucleotidique dans un echantillon d'adn
PCT/US2001/026611 WO2002016652A2 (fr) 2000-08-25 2001-08-24 Dispositif de traitement rapide d'echantillons d'adn, par manipulation, thermocyclage et purification integrees de liquides

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2001/026555 WO2002015949A2 (fr) 2000-08-25 2001-08-24 Dispositif pour l'identification d'une sequence nucleotidique dans un echantillon d'adn

Country Status (2)

Country Link
AU (2) AU2001286756A1 (fr)
WO (2) WO2002015949A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9062342B2 (en) 2012-03-16 2015-06-23 Stat-Diagnostica & Innovation, S.L. Test cartridge with integrated transfer module

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030077611A1 (en) 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
US7445893B2 (en) 2002-04-12 2008-11-04 Primera Biosystems, Inc. Sampling method for amplification reaction analysis
US7081339B2 (en) 2002-04-12 2006-07-25 Primera Biosystems, Inc. Methods for variation detection
CA2509769A1 (fr) * 2002-11-21 2004-06-10 Primera Biosystems Procede et dispositif d'echantillonnage permettant d'analyser des reactions d'amplification
EP2392402A3 (fr) * 2004-02-18 2012-03-21 Life Technologies Corporation Essais biologiques a etapes multiples sur des plates-formes d'applications micro-fluidiques modulaires
DE102005010096B3 (de) 2005-03-04 2006-11-09 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Assay mit osmotisch induzierter Abtrennung und Anreicherung hochmolekularer nachzuweisender Substanzen und fluidisches Mikrosystem zu seiner Durchführung
EP2012923A1 (fr) * 2006-04-07 2009-01-14 Corning Incorporated Microplaque fermée à écoulement continu et modes d'utilisation et procédés de fabrication de celle-ci
DE102007011866A1 (de) * 2007-03-08 2008-09-11 Friedrich-Schiller-Universität Jena Vorrichtung zur Aufnahme, Behandlung und Aufbewahrung kleinvolumiger Proben
WO2011009073A1 (fr) * 2009-07-16 2011-01-20 Abbott Laboratories Procédé d'amplification d'acides nucléiques
US20210316305A1 (en) * 2018-10-29 2021-10-14 Nok Corporation Microfluidic chip and microfluidic device
US20240100528A1 (en) * 2021-02-05 2024-03-28 Quantoom Biosciences France Sas Improved Thermocycled Multistep Reactions Device

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3591493A (en) * 1968-06-17 1971-07-06 Rashid A Zeineh Method for the treatment of biological fluids and apparatus therefor
EP0434149A2 (fr) * 1989-12-22 1991-06-26 Eastman Kodak Company Dispositif pour le transfert de liquides
US5672319A (en) * 1993-04-29 1997-09-30 Danfoss A/S Device for analyzing a fluid medium
US5733442A (en) * 1990-12-07 1998-03-31 Shukla; Ashok K. Microdialysis/Microelectrodialysis system
DE10005024A1 (de) * 2000-02-04 2001-08-23 Eppendorf Ag Dialysestopfen

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8629740D0 (en) * 1986-12-12 1987-01-21 Iq Bio Ltd Immunoassay
US5188963A (en) * 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
US5632957A (en) * 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
DE19950385A1 (de) * 1999-01-29 2000-08-03 Max Planck Gesellschaft Verfahren zur Isolation von Apoptose-induzierenden DNA-Sequenzen und Detektionssystem
AU2634600A (en) * 1999-01-29 2000-08-18 Genomic Instrumentation Services, Inc. Pipetter

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3591493A (en) * 1968-06-17 1971-07-06 Rashid A Zeineh Method for the treatment of biological fluids and apparatus therefor
EP0434149A2 (fr) * 1989-12-22 1991-06-26 Eastman Kodak Company Dispositif pour le transfert de liquides
US5733442A (en) * 1990-12-07 1998-03-31 Shukla; Ashok K. Microdialysis/Microelectrodialysis system
US5672319A (en) * 1993-04-29 1997-09-30 Danfoss A/S Device for analyzing a fluid medium
DE10005024A1 (de) * 2000-02-04 2001-08-23 Eppendorf Ag Dialysestopfen

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9062342B2 (en) 2012-03-16 2015-06-23 Stat-Diagnostica & Innovation, S.L. Test cartridge with integrated transfer module
US9334528B2 (en) 2012-03-16 2016-05-10 Stat-Diagnostica & Innovation, S.L. Test cartridge with integrated transfer module

Also Published As

Publication number Publication date
WO2002015949A3 (fr) 2002-10-03
WO2002015949A8 (fr) 2002-07-04
WO2002015949A2 (fr) 2002-02-28
WO2002015949A9 (fr) 2003-03-27
AU2001286756A1 (en) 2002-03-04
WO2002016652A2 (fr) 2002-02-28
AU2001286784A1 (en) 2002-03-04

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