WO2002015800A1 - Methode d'echantillonnage d'acide nucleique transdermique - Google Patents

Methode d'echantillonnage d'acide nucleique transdermique Download PDF

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Publication number
WO2002015800A1
WO2002015800A1 PCT/US2001/026102 US0126102W WO0215800A1 WO 2002015800 A1 WO2002015800 A1 WO 2002015800A1 US 0126102 W US0126102 W US 0126102W WO 0215800 A1 WO0215800 A1 WO 0215800A1
Authority
WO
WIPO (PCT)
Prior art keywords
skin
nucleic acid
microprojections
microprojection
piercing
Prior art date
Application number
PCT/US2001/026102
Other languages
English (en)
Inventor
James A. Matriano
Michel J. N. Cormier
Original Assignee
Alza Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alza Corporation filed Critical Alza Corporation
Priority to AU8346901A priority Critical patent/AU8346901A/xx
Priority to EP01962273A priority patent/EP1315459A1/fr
Priority to CA002420343A priority patent/CA2420343A1/fr
Priority to MXPA03001694A priority patent/MXPA03001694A/es
Priority to JP2002520715A priority patent/JP2004506914A/ja
Priority to AU2001283469A priority patent/AU2001283469B2/en
Priority to KR10-2003-7002654A priority patent/KR20030068127A/ko
Publication of WO2002015800A1 publication Critical patent/WO2002015800A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0045—Devices for taking samples of body liquids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
    • A61B17/20—Surgical instruments, devices or methods, e.g. tourniquets for vaccinating or cleaning the skin previous to the vaccination
    • A61B17/205—Vaccinating by means of needles or other puncturing devices
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0045—Devices for taking samples of body liquids
    • A61B2010/008—Interstitial fluid

Definitions

  • the sampling device used with this embodiment of the present invention can apply a partial vacuum (also referred to herein as "negative pressure") to the microcut skin.
  • the negative pressure causes interstitial fluid to efflux from the microcuts.
  • the interstitial fluid is collected, the nucleic acids contained therein is amplified using standard polymerase chain reaction (PCR) techniques, and then the amplified nucleic acids are analyzed.
  • the device for piercing the skin comprises a sheet having a plurality of openings therethrough, and a plurality of microprojections integral therewith and extending downward (i.e., in a direction toward the skin) therefrom.
  • the optional negative pressure driven device applies a partial vacuum (i.e., suction) to the microcuts through the openings in the sheet.
  • the microprojections should pierce into the skin to a depth of about 25 ⁇ m to about 400 ⁇ m, and preferably to a depth of about 50 ⁇ m to about 300 ⁇ m. Piercing to this depth with a plurality of microprojections ensures little if any bleeding, no significant sensation (pain) and further ensures that the blades will penetrate through the outermost o stratum corneum layer of dead cells and into the epidermis layer, or into both the epidermis and dermis layers, which contain live skin cells.
  • nucleic acid transfer/receiving layer coating the skin proximal side of plate 6.
  • transfer/receiving layers are disclosed in Theeuwes, et al. WO 98/28037, the disclosures of which are incorporated herein by reference.
  • the major barrier properties of the skin reside first with the walls of individual skin cells.
  • the microprojections in device 2 must be sized, shaped and configured to pierce through the stratum corneum layer and disrupt the cell walls of living skin cells in the layer of skin just below the stratum corneum layer, without piercing into the capillary beds within the skin so as to avoid significant bleeding.
  • metals and metal alloys include but are not limited to titanium, stainless steel, iron, steel, tin, zinc, copper, platinum, aluminum, germanium, nickel, zirconium, and titanium alloys consisting of nickel, molybdenum and chromium, metals plated with nickel, gold, rhodium, iridium, titanium, platinum, and the like.
  • glasses include silicon and a devitrified glass such as "Photoceram” available from Corning in Corning, NY.
  • rigid polymers include but are not limited to polystyrene, polymethylmethacrylate, polypropylene, polyethylene, "Bakelite", cellulose acetate, ethylcellulose, styrene/acrylonitrile copolymers, stryrenetbutadiene copolymers, acrylonitrile/butadiene/styrene (ABS) copolymers, polyvinyl chloride and acrylic acid polymers including polyacrylates and polymethacrylates.
  • ABS acrylonitrile/butadiene/styrene
  • the sheet and microprojections are made from titanium or stainless steel.

Abstract

L'invention concerne une méthode d'échantillonnage d'acide nucléique transdermique peu invasive, qui consiste à percer la couche supérieure de la peau (20) pour atteindre l'épiderme sous-jacent, à l'aide de plusieurs microprojections (4). Des cellules cutanées vivantes de l'épiderme sous-jacent sont désorganisées, ce qui les amène à libérer leurs contenus, notamment leurs acides nucléiques (c.-à-d. l'ADN, l'ARN, leurs fragments ou autres matières d'acide polynucléique trouvés dans les noyaux et/ou les mitochondries de cellules). L'acide nucléique est recueilli sur les surfaces des microprojections et/ou dans un réservoir de collecte d'acide nucléique séparé. L'acide nucléique recueilli est ensuite analysé par des techniques de réaction en chaîne de la polymérase (PCR). Facultativement, un dispositif d'aspiration (10) opère une dépression partielle à travers des ouvertures (8) ménagés dans l'élément à microprojections (6), jusqu'aux microcoupures pratiquées dans la peau (20), afin d'améliorer l'écoulement de liquides (c.-à-d. organiques) intracellulaires et extracellulaires contenant l'acide nucléique.
PCT/US2001/026102 2000-08-24 2001-08-20 Methode d'echantillonnage d'acide nucleique transdermique WO2002015800A1 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU8346901A AU8346901A (en) 2000-08-24 2001-08-20 Method for transdermal nucleic acid sampling
EP01962273A EP1315459A1 (fr) 2000-08-24 2001-08-20 Methode d'echantillonnage d'acide nucleique transdermique
CA002420343A CA2420343A1 (fr) 2000-08-24 2001-08-20 Methode d'echantillonnage d'acide nucleique transdermique
MXPA03001694A MXPA03001694A (es) 2000-08-24 2001-08-20 Metodo para toma de muestra transdermal de acido nucleico.
JP2002520715A JP2004506914A (ja) 2000-08-24 2001-08-20 経皮的核酸サンプリング方法
AU2001283469A AU2001283469B2 (en) 2000-08-24 2001-08-20 Method for transdermal nucleic acid sampling
KR10-2003-7002654A KR20030068127A (ko) 2000-08-24 2001-08-20 경피적 핵산 샘플링 방법

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22767500P 2000-08-24 2000-08-24
US60/227,675 2000-08-24

Publications (1)

Publication Number Publication Date
WO2002015800A1 true WO2002015800A1 (fr) 2002-02-28

Family

ID=22854028

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/026102 WO2002015800A1 (fr) 2000-08-24 2001-08-20 Methode d'echantillonnage d'acide nucleique transdermique

Country Status (8)

Country Link
EP (1) EP1315459A1 (fr)
JP (1) JP2004506914A (fr)
KR (1) KR20030068127A (fr)
CN (1) CN1471378A (fr)
AU (2) AU2001283469B2 (fr)
CA (1) CA2420343A1 (fr)
MX (1) MXPA03001694A (fr)
WO (1) WO2002015800A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005002453A1 (fr) * 2003-07-02 2005-01-13 Alza Corporation Timbre transdermique et methode d'immunisation mettant en oeuvre un reseau de microprojections
WO2009014766A2 (fr) * 2007-07-26 2009-01-29 The University Of Utah Research Foundation Récupération non invasive d'arn et analyse de l'expression génique dans la peau

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100367906C (zh) * 2004-12-08 2008-02-13 圣美迪诺医疗科技(湖州)有限公司 皮下植入式生物传感器
KR101032978B1 (ko) * 2009-04-29 2011-05-09 한국과학기술원 모션센서를 이용한 운동 피드백 기능과 운동기록이 가능한 아령세트

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3964482A (en) 1971-05-17 1976-06-22 Alza Corporation Drug delivery device
US5161532A (en) 1990-04-19 1992-11-10 Teknekron Sensor Development Corporation Integral interstitial fluid sensor
US5250023A (en) 1989-10-27 1993-10-05 Korean Research Institute on Chemical Technology Transdermal administration method of protein or peptide drug and its administration device thereof
US5279544A (en) 1990-12-13 1994-01-18 Sil Medics Ltd. Transdermal or interdermal drug delivery devices
US5320607A (en) 1992-02-13 1994-06-14 Kabushiki Kaisya Advance Simple blood sampling device
US5582184A (en) 1993-10-13 1996-12-10 Integ Incorporated Interstitial fluid collection and constituent measurement
US5682233A (en) 1995-09-08 1997-10-28 Integ, Inc. Interstitial fluid sampler
WO1997048440A1 (fr) 1996-06-18 1997-12-24 Alza Corporation Dispositif d'amelioration d'apport ou d'echantillonnage d'agents transdermiques
WO1998028037A1 (fr) * 1996-12-20 1998-07-02 Alza Corporation Dispositif et procede permettant d'ameliorer le flux d'agent transdermique
WO2000035530A1 (fr) * 1998-12-18 2000-06-22 Minimed Inc. Ensembles d'injection pourvus d'elements de micro-perforation, utilises avec des dispositifs medicaux, et leurs procedes d'utilisation
US6080172A (en) * 1997-05-30 2000-06-27 Nec Corporation Device for stabbing a corneum layer
US6091975A (en) 1998-04-01 2000-07-18 Alza Corporation Minimally invasive detecting device
WO2001041863A1 (fr) 1999-12-10 2001-06-14 Alza Corporation Dispositif et procede d'amelioration de percage de peau par micro-protuberances

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5250067A (en) * 1992-11-30 1993-10-05 Ala Gelfer Body treatment pad having a multiple number of sharpened skin-penetration protuberances
US6063082A (en) * 1997-11-04 2000-05-16 Scimed Life Systems, Inc. Percutaneous myocardial revascularization basket delivery system and radiofrequency therapeutic device
US6551338B1 (en) * 1999-09-01 2003-04-22 Mcgill University Method and device for myogenesis and angiogenesis of the heart

Patent Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3964482A (en) 1971-05-17 1976-06-22 Alza Corporation Drug delivery device
US5250023A (en) 1989-10-27 1993-10-05 Korean Research Institute on Chemical Technology Transdermal administration method of protein or peptide drug and its administration device thereof
US5161532A (en) 1990-04-19 1992-11-10 Teknekron Sensor Development Corporation Integral interstitial fluid sensor
US5279544A (en) 1990-12-13 1994-01-18 Sil Medics Ltd. Transdermal or interdermal drug delivery devices
US5320607A (en) 1992-02-13 1994-06-14 Kabushiki Kaisya Advance Simple blood sampling device
US5746217A (en) 1993-10-13 1998-05-05 Integ Incorporated Interstitial fluid collection and constituent measurement
US5582184A (en) 1993-10-13 1996-12-10 Integ Incorporated Interstitial fluid collection and constituent measurement
US5820570A (en) 1993-10-13 1998-10-13 Integ Incorporated Interstitial fluid collection and constituent measurement
US5682233A (en) 1995-09-08 1997-10-28 Integ, Inc. Interstitial fluid sampler
WO1997048441A1 (fr) 1996-06-18 1997-12-24 Alza Corporation Dispositif d'amelioration de prise d'echantillons transdermiques
WO1997048440A1 (fr) 1996-06-18 1997-12-24 Alza Corporation Dispositif d'amelioration d'apport ou d'echantillonnage d'agents transdermiques
WO1998028037A1 (fr) * 1996-12-20 1998-07-02 Alza Corporation Dispositif et procede permettant d'ameliorer le flux d'agent transdermique
US6080172A (en) * 1997-05-30 2000-06-27 Nec Corporation Device for stabbing a corneum layer
US6091975A (en) 1998-04-01 2000-07-18 Alza Corporation Minimally invasive detecting device
WO2000035530A1 (fr) * 1998-12-18 2000-06-22 Minimed Inc. Ensembles d'injection pourvus d'elements de micro-perforation, utilises avec des dispositifs medicaux, et leurs procedes d'utilisation
WO2001041863A1 (fr) 1999-12-10 2001-06-14 Alza Corporation Dispositif et procede d'amelioration de percage de peau par micro-protuberances

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1315459A1 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005002453A1 (fr) * 2003-07-02 2005-01-13 Alza Corporation Timbre transdermique et methode d'immunisation mettant en oeuvre un reseau de microprojections
WO2009014766A2 (fr) * 2007-07-26 2009-01-29 The University Of Utah Research Foundation Récupération non invasive d'arn et analyse de l'expression génique dans la peau
WO2009014766A3 (fr) * 2007-07-26 2009-04-09 Univ Utah Res Found Récupération non invasive d'arn et analyse de l'expression génique dans la peau
US8389215B2 (en) 2007-07-26 2013-03-05 University Of Utah Research Foundation Non-invasive recovery of RNA and analysis of gene expression in skin

Also Published As

Publication number Publication date
AU8346901A (en) 2002-03-04
CN1471378A (zh) 2004-01-28
JP2004506914A (ja) 2004-03-04
MXPA03001694A (es) 2004-11-01
KR20030068127A (ko) 2003-08-19
EP1315459A1 (fr) 2003-06-04
AU2001283469B2 (en) 2006-07-06
CA2420343A1 (fr) 2002-02-28

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