WO2002006469A3 - Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids - Google Patents

Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids Download PDF

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Publication number
WO2002006469A3
WO2002006469A3 PCT/US2001/022640 US0122640W WO0206469A3 WO 2002006469 A3 WO2002006469 A3 WO 2002006469A3 US 0122640 W US0122640 W US 0122640W WO 0206469 A3 WO0206469 A3 WO 0206469A3
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WIPO (PCT)
Prior art keywords
oligonucleotides
populations
methods
nucleic acids
population
Prior art date
Application number
PCT/US2001/022640
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French (fr)
Other versions
WO2002006469A2 (en
Inventor
Wayne M Coco
Lance P Encell
Joseph J Arensdorf
Original Assignee
Enchira Biotechnology Corp
Wayne M Coco
Lance P Encell
Joseph J Arensdorf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Enchira Biotechnology Corp, Wayne M Coco, Lance P Encell, Joseph J Arensdorf filed Critical Enchira Biotechnology Corp
Priority to AU2001273559A priority Critical patent/AU2001273559A1/en
Publication of WO2002006469A2 publication Critical patent/WO2002006469A2/en
Publication of WO2002006469A3 publication Critical patent/WO2002006469A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention is drawn to a method for forming at least one chimeric polynucleotide, methods for directed evolution, chimeric polynucleotides and libraries of chimeric polynucleotides. One method comprises contacting a first population of single-stranded oligonucleotides wherein the oligonucleotides share minimal complementarity with each other with a second population of oligonucleotides, under conditions wherein the oligonucleotides of the first and second populations hybridize to each other, forming at least one hybridized complex, comprising at least one polynucleotide from the first population hybridized to at least two oligonucleotides from the second population. Single-stranded regions are filled in using polymerase. The filled-in hybridized complex is treated such that the adjacent nucleic acids are ligated, forming at least one chimeric polynucleotide.
PCT/US2001/022640 2000-07-18 2001-07-18 Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids WO2002006469A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001273559A AU2001273559A1 (en) 2000-07-18 2001-07-18 Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US21892100P 2000-07-18 2000-07-18
US21908500P 2000-07-18 2000-07-18
US60/218,921 2000-07-18
US60/219,085 2000-07-18
US69187300A 2000-10-19 2000-10-19
US69273200A 2000-10-19 2000-10-19
US09/692,732 2000-10-19
US09/691,873 2000-10-19

Publications (2)

Publication Number Publication Date
WO2002006469A2 WO2002006469A2 (en) 2002-01-24
WO2002006469A3 true WO2002006469A3 (en) 2002-08-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/022640 WO2002006469A2 (en) 2000-07-18 2001-07-18 Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids

Country Status (2)

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AU (1) AU2001273559A1 (en)
WO (1) WO2002006469A2 (en)

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US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US6991922B2 (en) 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US20030036641A1 (en) * 2001-01-31 2003-02-20 Padgett Hal S. Methods for homology-driven reassembly of nucleic acid sequences
US20040132101A1 (en) 2002-09-27 2004-07-08 Xencor Optimized Fc variants and methods for their generation
GB2388604B (en) * 2002-05-17 2005-01-26 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
BRPI0314814C1 (en) 2002-09-27 2021-07-27 Xencor Inc antibody comprising an fc variant
JP2006500959A (en) * 2002-09-30 2006-01-12 パラレル バイオサイエンス， インコーポレイテッド Polynucleotide synthesis and labeling by dynamic sampling binding
JP2007527206A (en) * 2003-04-04 2007-09-27 ユニバーシティ オブ ローザンヌ Peptabody for cancer treatment
US8399618B2 (en) 2004-10-21 2013-03-19 Xencor, Inc. Immunoglobulin insertions, deletions, and substitutions
US8883147B2 (en) 2004-10-21 2014-11-11 Xencor, Inc. Immunoglobulins insertions, deletions, and substitutions
EP2221315A1 (en) 2003-12-04 2010-08-25 Xencor, Inc. Methods of generating variant proteins with increased host string content and compositions thereof
EP2053062A1 (en) 2004-03-24 2009-04-29 Xencor, Inc. Immunoglobin variants outside the Fc region
CN102746404B (en) 2004-11-12 2016-01-20 赞科股份有限公司 To FcRn in conjunction with reformed Fc variant
ATE425186T1 (en) 2005-01-05 2009-03-15 F Star Biotech Forsch & Entw SYNTHETIC IMMUNOGLOBULIN DOMAINS WITH BINDING PROPERTIES MODIFIED IN REGIONS OF THE MOLECULE DIFFERENT FROM THE AREAS DETERMINING COMPLEMENTARITY
GB0500703D0 (en) * 2005-01-14 2005-02-23 Bioinvent Int Ab Molecular biology method
AT503889B1 (en) 2006-07-05 2011-12-15 Star Biotechnologische Forschungs Und Entwicklungsges M B H F MULTIVALENT IMMUNE LOBULINE
JP5602625B2 (en) 2007-06-26 2014-10-08 エフ−スター ビオテヒノロギッシェ フォルシュングス− ウント エントヴィッケルングスゲゼルシャフト ミット ベシュレンクテル ハフツング Binding substance display
US7682809B2 (en) 2008-01-11 2010-03-23 Agilent Technologies, Inc. Direct ATP release sequencing
EP2113255A1 (en) 2008-05-02 2009-11-04 f-star Biotechnologische Forschungs- und Entwicklungsges.m.b.H. Cytotoxic immunoglobulin
EP3347466B1 (en) 2015-09-08 2024-01-03 Cold Spring Harbor Laboratory Genetic copy number determination using high throughput multiplex sequencing of smashed nucleotides

Citations (9)

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WO1994016090A1 (en) * 1993-01-15 1994-07-21 Molecular Tool, Inc. Method for generating single-stranded dna molecules
WO1995022625A1 (en) * 1994-02-17 1995-08-24 Affymax Technologies N.V. Dna mutagenesis by random fragmentation and reassembly
WO1998001581A1 (en) * 1996-07-09 1998-01-15 Recombinant Biocatalysis, Inc. Method of dna shuffling with polynucleotides produced by blockingor interrupting a synthesis or amplification process
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WO1998042832A1 (en) * 1997-03-25 1998-10-01 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
FR2782323A1 (en) * 1998-08-12 2000-02-18 Proteus In vitro recombination of polynucleotide fragments to obtain sequences with improved properties involves ligation on an assembly matrix
WO2000042561A2 (en) * 1999-01-19 2000-07-20 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
WO2001029212A1 (en) * 1999-10-19 2001-04-26 Enchira Biotechnology Corporation Methods for chimeragenesis of whole genomes or large polynucleotides
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Patent Citations (10)

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WO1994016090A1 (en) * 1993-01-15 1994-07-21 Molecular Tool, Inc. Method for generating single-stranded dna molecules
WO1995022625A1 (en) * 1994-02-17 1995-08-24 Affymax Technologies N.V. Dna mutagenesis by random fragmentation and reassembly
WO1998001581A1 (en) * 1996-07-09 1998-01-15 Recombinant Biocatalysis, Inc. Method of dna shuffling with polynucleotides produced by blockingor interrupting a synthesis or amplification process
WO1998027230A1 (en) * 1996-12-18 1998-06-25 Maxygen, Inc. Methods and compositions for polypeptide engineering
WO1998042832A1 (en) * 1997-03-25 1998-10-01 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
WO1998042728A1 (en) * 1997-03-25 1998-10-01 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
FR2782323A1 (en) * 1998-08-12 2000-02-18 Proteus In vitro recombination of polynucleotide fragments to obtain sequences with improved properties involves ligation on an assembly matrix
WO2000042561A2 (en) * 1999-01-19 2000-07-20 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
WO2001029212A1 (en) * 1999-10-19 2001-04-26 Enchira Biotechnology Corporation Methods for chimeragenesis of whole genomes or large polynucleotides
WO2001029211A2 (en) * 1999-10-19 2001-04-26 Enchira Biotechnology Corporation Method for directed evolution by random chimeragenesis on transient templates

Non-Patent Citations (5)

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Title
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CRAMERI A ET AL: "DNA SHUFFLING OF A FAMILY OF GENES FROM DIVERSE SPECIES ACCELERATESDIRECTED EVOLUTION", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 391, 15 January 1998 (1998-01-15), pages 288 - 291, XP000775869, ISSN: 0028-0836 *
KIKUCHI M ET AL: "Novel family shuffling methods for the in vitro evolution of enzymes", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 236, no. 1, 5 August 1999 (1999-08-05), pages 159 - 167, XP004175459, ISSN: 0378-1119 *
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WO2002006469A2 (en) 2002-01-24

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