DK0645449T3 - Process for Specific Cloning of Nucleic Acids - Google Patents

Process for Specific Cloning of Nucleic Acids

Info

Publication number
DK0645449T3
DK0645449T3 DK94114675T DK94114675T DK0645449T3 DK 0645449 T3 DK0645449 T3 DK 0645449T3 DK 94114675 T DK94114675 T DK 94114675T DK 94114675 T DK94114675 T DK 94114675T DK 0645449 T3 DK0645449 T3 DK 0645449T3
Authority
DK
Denmark
Prior art keywords
nucleic acids
specific cloning
ligated
cloning
strand
Prior art date
Application number
DK94114675T
Other languages
Danish (da)
Inventor
Wolf Dr Bertling
Original Assignee
November Ag Molekulare Medizin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by November Ag Molekulare Medizin filed Critical November Ag Molekulare Medizin
Application granted granted Critical
Publication of DK0645449T3 publication Critical patent/DK0645449T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

The invention relates to a method for the cloning of nucleic acids, characterised in that two partly complementary, double-stranded primers are ligated to the 3' end of a single-stranded DNA strand. The second strand is synthesised by means of a DNA polymerase, with the primers used being at least partly complementary with the oligonucleotide which is ligated on. The target DNA obtained in this way can then be specifically amplified with two specific oligonucleotides. <IMAGE>
DK94114675T 1993-09-24 1994-09-17 Process for Specific Cloning of Nucleic Acids DK0645449T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4332463A DE4332463A1 (en) 1993-09-24 1993-09-24 Process for the specific cloning of nucleic acids

Publications (1)

Publication Number Publication Date
DK0645449T3 true DK0645449T3 (en) 2002-05-21

Family

ID=6498466

Family Applications (1)

Application Number Title Priority Date Filing Date
DK94114675T DK0645449T3 (en) 1993-09-24 1994-09-17 Process for Specific Cloning of Nucleic Acids

Country Status (6)

Country Link
EP (1) EP0645449B1 (en)
JP (1) JPH07177885A (en)
AT (1) ATE214096T1 (en)
DE (2) DE4332463A1 (en)
DK (1) DK0645449T3 (en)
ES (1) ES2173897T3 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2206451C (en) * 1994-12-09 2002-11-26 Wakunaga Seiyaku Kabushiki Kaisha Method for suppressing nonspecific hybridization in primer extension method
US5753462A (en) * 1995-06-07 1998-05-19 Zymogenetics, Inc. Secretion leader trap cloning method
JPH11506939A (en) * 1995-06-07 1999-06-22 ザイモジェネティクス，インコーポレイティド Secretory leader trap cloning
DE19601385A1 (en) * 1996-01-16 1997-07-17 Mueller Manfred W Method for amplifying nucleic acid
WO1998051699A1 (en) * 1997-05-12 1998-11-19 Life Technologies, Inc. Methods for production and purification of nucleic acid molecules
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
US6368834B1 (en) * 1999-04-06 2002-04-09 Genome Technologies, Llc PCR genome walking with synthetic primer
US6083727A (en) * 1999-06-30 2000-07-04 Incyte Pharmaceuticals Inc. Methods and compositions for producing 5' enriched cDNA libraries
EP1325118B1 (en) 2000-10-05 2016-05-25 Riken Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers.

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4666839A (en) * 1982-12-01 1987-05-19 Amgen Methods and materials for obtaining microbial expression of polypeptides including bovine prolactin
DE3929030A1 (en) * 1989-09-01 1991-03-07 Boehringer Mannheim Gmbh METHOD FOR PROPELLING NUCLEIC ACIDS
GB9011454D0 (en) * 1990-05-22 1990-07-11 Medical Res Council Polynucleotide amplification
FR2678639B1 (en) * 1991-07-03 1993-09-17 Rhone Poulenc Rorer Sa PROCESS FOR CLONING NUCLEIC ACIDS.

Also Published As

Publication number Publication date
DE59410068D1 (en) 2002-04-11
ES2173897T3 (en) 2002-11-01
JPH07177885A (en) 1995-07-18
EP0645449A1 (en) 1995-03-29
EP0645449B1 (en) 2002-03-06
ATE214096T1 (en) 2002-03-15
DE4332463A1 (en) 1995-03-30

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