WO2001091702A1 - Produit cosmetique - Google Patents

Produit cosmetique Download PDF

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Publication number
WO2001091702A1
WO2001091702A1 PCT/JP2000/003457 JP0003457W WO0191702A1 WO 2001091702 A1 WO2001091702 A1 WO 2001091702A1 JP 0003457 W JP0003457 W JP 0003457W WO 0191702 A1 WO0191702 A1 WO 0191702A1
Authority
WO
WIPO (PCT)
Prior art keywords
water
silk
protein
acid
cosmetic
Prior art date
Application number
PCT/JP2000/003457
Other languages
English (en)
Japanese (ja)
Inventor
Kiyoshi Hirabayashi
Original Assignee
Road Nijuuichi Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Road Nijuuichi Co., Ltd. filed Critical Road Nijuuichi Co., Ltd.
Priority to AU47828/00A priority Critical patent/AU4782800A/en
Priority to PCT/JP2000/003457 priority patent/WO2001091702A1/fr
Publication of WO2001091702A1 publication Critical patent/WO2001091702A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations

Definitions

  • the present invention relates to a cosmetic that contains a water-soluble protein derived from silk protein, contains an ultraviolet absorbent that does not irritate the skin, and has a good feeling in use.
  • the ultraviolet blocking substances contained in these cosmetics include fine particle ultraviolet scattering agents such as titanium dioxide and zinc oxide, P-aminobenzoic acids, hydroxybenzophenones, cinnamic acids, benzofurans, salicylic acids, coumarins. And ultraviolet absorbing compounds such as azoles are well known.
  • fine particle ultraviolet scattering agents such as titanium dioxide and zinc oxide
  • P-aminobenzoic acids such as hydroxybenzophenones, cinnamic acids, benzofurans, salicylic acids, coumarins.
  • ultraviolet absorbing compounds such as azoles are well known.
  • those that can be used for cosmetics are limited.
  • titanium dioxide, acid Fine particles, such as zinc oxide have a very high UV scattering effect.However, when the amount is too large, the feeling of use decreases, such as roughness and poor elongation, and the skin becomes whitish and finishes as a cosmetic. There is a problem. It may also act as a photocatalyst on the surface
  • UV-B having a shorter wavelength than 320 nm has a more adverse effect on the skin than UV-A having a longer wavelength than 320 nm.
  • the UV-absorbing compound is not necessarily sufficient in terms of UV-B absorption.
  • the present invention has been made in such a situation, and it is an object of the present invention to provide a cosmetic product that contains an ultraviolet ray Kaizumi absorbent that does not irritate the skin and has a good feeling of use.
  • the inventors of the present invention have conducted intensive studies to solve the above-mentioned problems, and have found that tyrosine and phenylalanine contained in silk protein are excellent in UV-UV absorption even among ultraviolet rays.
  • the hydrolyzed silk protein can also be used as food, and there is no need to worry about adverse effects on the human body.It is water-soluble and can be suitably used as cosmetics.
  • the present inventors have found that the above problems can be solved at once if a water-soluble protein derived from silk containing tyrosine is included in cosmetics, and have completed the present invention. That is, the present invention is a cosmetic product having an ultraviolet-ray-preventing effect and containing, as an active ingredient, a water-soluble protein containing at least tyrosine, which is a hydrolyzate of silk protein.
  • the average molecular weight of the water-soluble protein is usually about 150 to 500,000.
  • the water-soluble protein usually further contains phenylalanine.
  • the water-soluble protein is desirably contained in an amount of 3% by mass or more based on 100% by mass of the cosmetic.
  • the silk protein is preferably a silk-five mouth in-protein.
  • FIG. 1 is a diagram showing a test material for testing the ultraviolet absorption effect of silk protein.
  • FIG. 2 is a diagram showing test results of the ultraviolet absorption effect.
  • FIG. 3 is a diagram showing a UV-B ultraviolet absorption spectrum.
  • the cosmetic according to the present invention contains, as an active ingredient, a water-soluble protein containing at least tyrosine, which is a hydrolyzate of silk protein.
  • the hydrolyzate of the silk protein contained in the cosmetic of the present invention is obtained by hydrolyzing, preferably partially hydrolyzing the silk protein so that the amount of free amino acid produced is 1 to 70%.
  • the silk protein raw material to be subjected to the above hydrolysis cocoon balls, silk yarn, etc. can be used, and it is preferable to use yarn waste generated in the spinning, weaving process, etc. from the viewpoint of both waste treatment and economy.
  • Silk is a fibrous protein secreted from the silk glands of silkworms, and fibrous mouth fibers are hardened with sericin.
  • fiber-in fiber When fiber-in fiber is used as the silk protein, sericin can be removed by a known refining method to obtain fiber-in fiber.For example, treatment such as dissolution, precipitation, extraction, purification, and culture from cocoons or silk thread can be performed. It is obtained by performing.
  • Hydrolysis of silk proteins can be performed using acids, alkalis or enzymes.
  • the above silk fiber mouth-in fiber is heated and dissolved in a concentrated solution of a mineral acid selected from hydrochloric acid (hereinafter sometimes referred to as a hydrochloric acid method), sulfuric acid, phosphoric acid, and the like, and then hydrolyzed. After neutralization and desalting, a water-soluble protein derived from silk protein can be obtained.
  • a hydrochloric acid method hydrochloric acid
  • sulfuric acid sulfuric acid
  • phosphoric acid sulfuric acid
  • a water-soluble protein derived from silk protein can be obtained.
  • the above silk fibroin fiber is mixed with a 45% calcium chloride solution or a 1: 2: 8 (weight ratio) solution of calcium chloride, ethanol and water (these are referred to as calcium chloride method).
  • this solution can be obtained by desalting.
  • a water-soluble protein derived from a silk protein having a small molecular weight can be obtained.
  • water-soluble protein obtained above is dried and powdered by a method such as spray drying or freeze drying, a water-soluble protein powder derived from silk protein can be obtained.
  • the hydrolysis is preferably partially hydrolyzed so that the average molecular weight of the protein is usually about 150 to 500,000, preferably about 300 to 300.000. If the average molecular weight is larger than 500, 000, it has a gelling ability, so that the processability in preparing the cosmetic composition tends to be inferior.
  • a water-soluble protein having an average molecular weight of about 300 it is particularly preferable to use a water-soluble protein having an average molecular weight of about 300, and a cosmetic having a good feeling in use can be obtained.
  • the amount of free amino acids produced after hydrolysis is usually preferably about 1 to 70% (by mass) of the fibroin fiber as a raw material.
  • the amount of free amino acids generated in the water-soluble protein solution was determined by treating the water-soluble protein solution with a basic protein solution with basic copper carbonate, converting the amino acid and the peptide into a copper complex, and adsorbing this to an anion exchange resin. It can be analyzed by quantifying free amino acids eluted with a 0.05 M borate buffer using an automatic amino acid analyzer. Acidic amino acids that are not released by the borate buffer can be analyzed by using an amino acid analyzer with the water-soluble protein solution as it is.
  • amino acid composition of silk fibroin fiber is shown below.
  • the water-soluble protein used in the present invention contains the amino acid component of the silk protein and at least tyrosine. Further, it is desirable to include fenilalanine.
  • the cosmetic of the present invention contains a water-soluble protein containing at least tyrosine as described above as an active ingredient.
  • the content of the water-soluble protein varies depending on the form of use of the cosmetic, but is usually 3% by mass or more in the total 100% by mass of the cosmetic.
  • the cosmetics according to the present invention may be used in the form of a liquid (lotion), an emulsion, an oily liquid, a paste, an oil-in-water emulsion, a water-in-oil emulsion, a cream, a wax, a gel, a solid, It may be in any of a powder form, a powder solid form, an oily solid form, and a multilayer form, and is not particularly limited.
  • sunscreen cosmetics such as sunscreen creams, moisturizing jewels such as lotions, lotions, and serums, white powder, foundations, shampoos
  • Hair care products such as hair care, hair conditioners, hair treatments, hair creams, hair sprays, hair styling agents, stones, liquid stones, cleansing creams, cleansing jewels, etc.
  • hair styling agents such as hairs, massage creams and lipsticks.
  • the cosmetic of the present invention can optionally contain various additives commonly used in cosmetics as long as the effects of the present invention are not impaired, and optional components are appropriately used according to each use. be able to.
  • Examples of general components to be added when forming such cosmetics include, more specifically, oil components, aqueous components, humectants, amino acids and their derivatives, anti-fading agents, antioxidants, and surfactants. , Defoamer, beauty and whitening ingredients, other UV absorbers, powder ingredients, preservatives, coloring agents, fragrances, moisturizers, enzymes, anti-inflammatory agents, bactericides, preservatives, antioxidants, chelating agents, Antiperspirants, pigments, inorganic compounds, pH regulators, pearlizing agents, wetting agents, astringents, cosmetics varieties, standardization of quasi-drug ingredients, Japanese Pharmacopoeia, food additives Can be used.
  • the oil component may be any of solid, semi-solid, liquid, volatile oil, etc., and if it can be used for cosmetics, it may be animal, plant, etc. It may be any of synthetic or synthetic, such as hydrocarbons, oils and fats, waxes, hardened oils, ester oils, fatty acids, higher alcohols, silicone oils, fluorine-based oils, lanolin derivatives, Oily gelling agents and the like can be used.
  • aqueous component examples include water and water-soluble components that can be used for cosmetics.
  • water-soluble component examples include alcohols such as ethyl alcohol and isopropyl alcohol, propylene glycol, 1,3-butylene glycol, and dipropylene glycol.
  • surfactant those which can be used for cosmetics can be widely used.
  • nonionic surfactants anionic surfactants, cationic surfactants, amphoteric surfactants, and the like can be used.
  • Nonionic surfactants include, for example, dariserin fatty acid esters and their alkylene dalicol adducts, polyglycerin fatty acid esters and their alkylene glycol adducts, propylene glycol fatty acid esters and their alkylene glycol adducts, sorbitan fatty acid esters and their Alkylene glycol adducts, fatty acid esters of sorbitol and their alkylene diglycol adducts, polyalkylene glycol fatty acid esters, sucrose fatty acid esters, polyoxyalkylene alkyl ethers, glycerin alkyl ethers, polyoxyethylene alkyl phenyl ethers, poly Oxyethylene hydrogenated castor oil, adduct of lanolin with alkylenedaricol, polyoxyalkylene-modified silicone, polyether And modified silicone.
  • anionic surfactants include inorganic and organic salts of fatty acids such as stearic acid and lauric acid, alkylbenzene sulfates, alkyl sulfonates, monoolefin sulfonates, dialkyl sulfosuccinates, ⁇ -sulfonates Fatty acid salts, acyl methyl taurine salts, polymethyl mono-alkyl taurine salts, polyoxetylene alkyl ether sulfates, polyoxetylene alkyl phenyl ether sulfates, alkyl phosphates, polyoxyethylene alkyl ethers Phosphate, polyoxyethylene alkyl phenyl ether phosphate, ⁇ -amino acid salt, ⁇ -amino acid salt, 0-alkyl-substituted malate, alkyls And rufosuccinate.
  • fatty acids such as stearic acid and lauric acid
  • Examples of the cationic surfactant include an alkylamine salt, a polyamine and an alkanolamine fatty acid derivative, an alkyl quaternary ammonium salt, and a cyclic quaternary ammonium salt.
  • Examples of amphoteric surfactants include phospholipid-containing components such as lecithin and lysolecithin and their hydrogenated products, carboxylic acid type, amino acid type, and bayone type, sulfate ester type, sulfonate type, and phosphate ester type. There are things that can be used for humans.
  • N N-dimethyl-1-N-alkyl-1-N-hydroxypropylammonium betaine
  • N N-dialkylaminoalkylenecarbonic acid
  • N N, N, N-trialkyl-1-N-sulfoalkyleneammonium betaine Tyne
  • N, N-dialkyl-N N-bis (polyoxyethylene sulfate) ammonium betaine
  • 2-alkyl-1-hydroxyethyl-1 lipoxymethylimidazolinium betaine etc.
  • UV absorbers for cosmetics may be used.
  • para-aminobenzoic acid ethyl para-aminobenzoate, glyceryl para-aminobenzoate, para-dimethylamino PABAs such as amyl benzoate, paradimethylamino-1-ethylhexyl benzoate, para-dihydroxypropyl ethyl benzoate, etc.
  • p-methoxy cinnamate 1-2-ethyl hexyl 4-methoxy cinnamate-2-ethoxyxetil, etc.
  • Salicylic acids such as citric acid, 2-ethylhexyl salicylate, phenyl salicylate, homomenthyl salicylate, 2-hydroxy-14-methoxybenzophenone, 2-hydroxy-14-methoxybenzophenone-15-sulfonic acid, 2-Hydroxy-1-methoxybenzofenone 5-Sulfo Sodium acid, 2, 2 '- dihydroxy - 4, 4' - Dimethoxybenzophenone, 2,2'-dihydroxy-1,4'-dimethoxybenzophenone-15-sodium sulfonate, 2,4-dihydroxybenzophenone, 2,2 ', 4,4'-tetrahydroxybenzo Benzophenone-based compounds such as phenone, 2_ (2-hydroxy_5-methylphenyl) benzotriazole, 4_tert-butyl-4'-methoxydibenzoylmethane, oxybenzone and the like can be mentioned.
  • amino acid derivatives such as sodium pyrrolidonecarboxylate, other proteins, moisturizing agents such as mucopolysaccharide, collagen, elastin, and keratin; antioxidants such as tocopherol, ascorbic acid and esters or salts thereof; , Anti-inflammatory agents, herbal medicines and other beauty and whitening ingredients, paraoxybenzoic acid esters, phenoxyethanol and other preservatives, magnesium silicate, aluminum magnesium silicate, talc, kaolin, bentonite, my power, potato black,
  • examples include inorganic pigments such as calamine, astringents such as lactic acid, tartaric acid, succinic acid, cunic acid, and allantoin, and anti-inflammatory agents such as guaiazulene and methyl salicylate.
  • a test material as shown in Fig. 1 was prepared, and the UV absorption test of the hydrolyzate of silk protein was done.
  • test sample cream was prepared by adding 10% (mass ratio) of each powder of silk, wool, chitin and chitosan to cold cream (commercially available product).
  • the silk here is a powder obtained by drying a relatively high molecular weight water-soluble silk protein obtained by hydrolyzing silk fibroin by a calcium chloride method.
  • a silk cloth (Habuta) was fixed on cardboard, and the above-mentioned test sample cream of the same mass was applied to each area of 3 cm x 3 cm.
  • test material was irradiated with ultraviolet light (1.5 kW) of 254 nm from the upper lcm for 24 hours. After irradiation, the cream on the silk cloth was washed off, and the yellowing degree was measured using a measuring instrument (manufactured by Tokyo Kogaku).
  • the yellowed silk cloth was hydrolyzed with hydrochloric acid to obtain amino acids. After standing, a tan precipitate was separated from the clear, colorless supernatant and dried. Amino acid analysis revealed that 70% or more was tyrosine. In addition, feniralanine was also confirmed.
  • the ultraviolet absorption effect of silk was at least due to tyrosine. It is particularly effective in absorbing ultraviolet rays B (below 300 nm), which are harmful to the skin.
  • the solid line is the ultraviolet absorption spectrum of silk
  • the dotted line is the ultraviolet absorption spectrum of tyrosine.
  • the present invention it is possible to provide a cosmetic product having an ultraviolet ray preventing effect, excellent usability and a feeling of use, and having no skin irritation.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Zoology (AREA)
  • Dermatology (AREA)
  • Cosmetics (AREA)

Abstract

L'invention concerne un produit cosmétique possédant un effet protecteur contre les rayons ultraviolets. Ce produit présente d'excellentes caractéristiques d'utilisation, une texture agréable, et n'irrite pas la peau. Ce produit contient en tant que principe actif une protéine hydrosoluble qui est un produit d'hydrolyse de protéines de soie, de la tyrosine et éventuellement d'autres composants.
PCT/JP2000/003457 2000-05-29 2000-05-29 Produit cosmetique WO2001091702A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU47828/00A AU4782800A (en) 2000-05-29 2000-05-29 Cosmetic
PCT/JP2000/003457 WO2001091702A1 (fr) 2000-05-29 2000-05-29 Produit cosmetique

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2000/003457 WO2001091702A1 (fr) 2000-05-29 2000-05-29 Produit cosmetique

Publications (1)

Publication Number Publication Date
WO2001091702A1 true WO2001091702A1 (fr) 2001-12-06

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PCT/JP2000/003457 WO2001091702A1 (fr) 2000-05-29 2000-05-29 Produit cosmetique

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AU (1) AU4782800A (fr)
WO (1) WO2001091702A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112932989A (zh) * 2021-03-17 2021-06-11 广州花出见生物科技有限公司 一种蚕丝蛋白滋润粉底液及其制备方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06292595A (ja) * 1993-04-05 1994-10-21 Kiyoshi Hirabayashi 低分子量フィブロインの製造方法
JP2000053551A (ja) * 1998-08-10 2000-02-22 Nikko:Kk 紫外線吸収剤および化粧品用配合剤

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06292595A (ja) * 1993-04-05 1994-10-21 Kiyoshi Hirabayashi 低分子量フィブロインの製造方法
JP2000053551A (ja) * 1998-08-10 2000-02-22 Nikko:Kk 紫外線吸収剤および化粧品用配合剤

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112932989A (zh) * 2021-03-17 2021-06-11 广州花出见生物科技有限公司 一种蚕丝蛋白滋润粉底液及其制备方法

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Publication number Publication date
AU4782800A (en) 2001-12-11

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