WO2001084146A3 - Biocapteur multiplex sensible a spectrometrie de masse base sur l'immuno-pcr - Google Patents

Biocapteur multiplex sensible a spectrometrie de masse base sur l'immuno-pcr Download PDF

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Publication number
WO2001084146A3
WO2001084146A3 PCT/IB2001/000957 IB0100957W WO0184146A3 WO 2001084146 A3 WO2001084146 A3 WO 2001084146A3 IB 0100957 W IB0100957 W IB 0100957W WO 0184146 A3 WO0184146 A3 WO 0184146A3
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WO
WIPO (PCT)
Prior art keywords
spectrometry
immuno
pcr
sensitive
mass
Prior art date
Application number
PCT/IB2001/000957
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English (en)
Other versions
WO2001084146B1 (fr
WO2001084146A2 (fr
Inventor
Curt T Reimann
Original Assignee
Forskarpatent I Syd Ab
Curt T Reimann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forskarpatent I Syd Ab, Curt T Reimann filed Critical Forskarpatent I Syd Ab
Priority to AU2001258700A priority Critical patent/AU2001258700A1/en
Publication of WO2001084146A2 publication Critical patent/WO2001084146A2/fr
Publication of WO2001084146A3 publication Critical patent/WO2001084146A3/fr
Publication of WO2001084146B1 publication Critical patent/WO2001084146B1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Wood Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

La présente invention concerne un procédé de détection de substances, selon lequel on procède à la détection immunologique d'une substance, on amplifie ensuite un acide nucléique marqueur lié à un anticorps, et on identifie le marqueur amplifié. Le procédé de détection de l'invention fait appel à la spectrométrie de masse qui, en décelant la présence et en identifiant la masse de l'acide nucléique marqueur, permet de détecter la liaison spécifique de l'anticorps avec un antigène.
PCT/IB2001/000957 2000-05-04 2001-05-04 Biocapteur multiplex sensible a spectrometrie de masse base sur l'immuno-pcr WO2001084146A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001258700A AU2001258700A1 (en) 2000-05-04 2001-05-04 A sensitive, multiplexed mass-spectrometry-based biosensor based on immuno-pcr

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE0001670A SE0001670D0 (sv) 2000-05-04 2000-05-04 Mass-spectrometry-based biosensor
SE0001670-9 2000-05-04

Publications (3)

Publication Number Publication Date
WO2001084146A2 WO2001084146A2 (fr) 2001-11-08
WO2001084146A3 true WO2001084146A3 (fr) 2002-04-11
WO2001084146B1 WO2001084146B1 (fr) 2002-07-04

Family

ID=20279569

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2001/000957 WO2001084146A2 (fr) 2000-05-04 2001-05-04 Biocapteur multiplex sensible a spectrometrie de masse base sur l'immuno-pcr

Country Status (3)

Country Link
AU (1) AU2001258700A1 (fr)
SE (1) SE0001670D0 (fr)
WO (1) WO2001084146A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001078652A2 (fr) 2000-04-14 2001-10-25 Metabolon, Inc. Procedes permettant de decouvrir des medicaments, de traiter des maladies et de poser des diagnostics a l'aide de la metabolomique
US7329489B2 (en) 2000-04-14 2008-02-12 Matabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
DE10200374A1 (de) * 2002-01-08 2003-07-24 Friz Biochem Gmbh Verfahren zur qualitativ und quantitativ vergleichenden Detektion chemischer Substanzen
WO2004094456A2 (fr) * 2003-04-18 2004-11-04 Becton, Dickinson And Company Amplification immunologique
EP1753873A4 (fr) 2004-03-05 2008-11-05 Ohio Med College Procedes et compositions pour l'evaluation d'acides nucleiques et d'alleles
EP1745157A4 (fr) * 2004-04-12 2008-06-11 Ohio Med College Methodes et compositions pour analyser des analytes
WO2006128010A2 (fr) * 2005-05-26 2006-11-30 The Trustees Of Boston University Quantification d'acides nucleiques et de proteines au moyen d'etiquettes de masse d'oligonucleotides
US8849577B2 (en) 2006-09-15 2014-09-30 Metabolon, Inc. Methods of identifying biochemical pathways
JP2009219388A (ja) * 2008-03-14 2009-10-01 Jsr Corp イムノｐｃｒ用磁性粒子、標的物質の検出方法、および標的物質の検出キット
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
KR20220038550A (ko) * 2013-03-13 2022-03-28 메소 스케일 테크놀러지즈, 엘엘시 개선된 분석 방법
WO2015175856A1 (fr) 2014-05-15 2015-11-19 Meso Scale Technologies, Llc. Méthodes de dosage améliorées
CA3043639A1 (fr) 2016-12-09 2018-06-14 Ultivue, Inc. Procedes ameliores d'imagerie multiplex utilisant des agents d'imagerie d'acides nucleiques marques
WO2018183876A1 (fr) 2017-03-31 2018-10-04 Ultivue, Inc. Échange adn-antigène et amplification
WO2020131182A2 (fr) * 2018-09-26 2020-06-25 The University Of North Carolina At Chapel Hill Composés, compositions et méthodes d'amélioration de dosages

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996029432A1 (fr) * 1995-03-22 1996-09-26 Trustees Of Boston University Procedes de detection de paracoccidioides
WO1999038013A2 (fr) * 1998-01-23 1999-07-29 Xerion Pharmaceuticals Gmbh Procede permettant d'identifier simultanement des proteines et leurs partenaires de liaison
WO2000057183A1 (fr) * 1999-03-23 2000-09-28 Biovation Limited Isolement et analyse de proteine

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996029432A1 (fr) * 1995-03-22 1996-09-26 Trustees Of Boston University Procedes de detection de paracoccidioides
WO1999038013A2 (fr) * 1998-01-23 1999-07-29 Xerion Pharmaceuticals Gmbh Procede permettant d'identifier simultanement des proteines et leurs partenaires de liaison
WO2000057183A1 (fr) * 1999-03-23 2000-09-28 Biovation Limited Isolement et analyse de proteine

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE BIOSIS, PREV199800180103 January 1998 (1998-01-01), JURINKE CHRISTINA ET AL: "Application of nested PCR and mass spectrometry for DNA-based virus detection: HBV-DNA detected in the majority of isolated anti-HBc positive sera", XP002901936 *
MARIAN C CASE ET AL: "Enhanced ultrasensitive detection of structurally diverse antigens using a single immuno-PCR assay protocol", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 223, 1999, pages 93 - 106, XP002901935 *

Also Published As

Publication number Publication date
WO2001084146B1 (fr) 2002-07-04
AU2001258700A1 (en) 2001-11-12
WO2001084146A2 (fr) 2001-11-08
SE0001670D0 (sv) 2000-05-04

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