WO2002033410A3 - Essais d'immuno-absorbant en microvolume avec détection électrochimique amplifiée - Google Patents

Essais d'immuno-absorbant en microvolume avec détection électrochimique amplifiée Download PDF

Info

Publication number
WO2002033410A3
WO2002033410A3 PCT/US2001/032552 US0132552W WO0233410A3 WO 2002033410 A3 WO2002033410 A3 WO 2002033410A3 US 0132552 W US0132552 W US 0132552W WO 0233410 A3 WO0233410 A3 WO 0233410A3
Authority
WO
WIPO (PCT)
Prior art keywords
electrochemical detection
microvolume
combined
assay techniques
assays
Prior art date
Application number
PCT/US2001/032552
Other languages
English (en)
Other versions
WO2002033410A2 (fr
Inventor
Ingrid Fritsch
Robert Beittle Jr
Original Assignee
Univ Arkansas
Ingrid Fritsch
Robert Beittle Jr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Arkansas, Ingrid Fritsch, Robert Beittle Jr filed Critical Univ Arkansas
Priority to EP01983168A priority Critical patent/EP1326712A2/fr
Priority to AU1461702A priority patent/AU1461702A/xx
Publication of WO2002033410A2 publication Critical patent/WO2002033410A2/fr
Publication of WO2002033410A3 publication Critical patent/WO2002033410A3/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3032—Micromixers using magneto-hydrodynamic [MHD] phenomena to mix or move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B17/00—Pumps characterised by combination with, or adaptation to, specific driving engines or motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00511—Walls of reactor vessels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B01J2219/00704—Processes involving means for analysing and characterising the products integrated with the reactor apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/607—Detection means characterised by use of a special device being a sensor, e.g. electrode

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Electrochemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne une structure et un procédé de détection chimique par micro-essais. En combinant aux microcavités ou aux micropores sont combinés des techniques d'essai, on arrive à une détection très rapide et très sensible de composés chimiques. On a fait évoluer les techniques d'essais pour qu'elles acceptent une espèce porteuse se liant à un ion métal convenant particulièrement en liaison avec la détection électrochimique. Cela permet de combiner à l'analyse électrochimique les essais, d'où la commodité de la grande vitesse et l'hypersensibilité de l'invention.
PCT/US2001/032552 2000-10-16 2001-10-16 Essais d'immuno-absorbant en microvolume avec détection électrochimique amplifiée WO2002033410A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01983168A EP1326712A2 (fr) 2000-10-16 2001-10-16 Essais d'immuno-absorbant en microvolume avec d tection lectrochimique amplifi e
AU1461702A AU1461702A (en) 2000-10-16 2001-10-16 Microvolume immunoabsorbant assays with amplified electrochemical detection

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US24069100P 2000-10-16 2000-10-16
US60/240,691 2000-10-16
US09/978,734 2001-10-15
US09/978,734 US6887714B2 (en) 2000-10-16 2001-10-15 Microvolume immunoabsorbant assays with amplified electrochemical detection

Publications (2)

Publication Number Publication Date
WO2002033410A2 WO2002033410A2 (fr) 2002-04-25
WO2002033410A3 true WO2002033410A3 (fr) 2002-07-04

Family

ID=26933626

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/032552 WO2002033410A2 (fr) 2000-10-16 2001-10-16 Essais d'immuno-absorbant en microvolume avec détection électrochimique amplifiée

Country Status (4)

Country Link
US (1) US6887714B2 (fr)
EP (1) EP1326712A2 (fr)
AU (1) AU1461702A (fr)
WO (1) WO2002033410A2 (fr)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7348183B2 (en) 2000-10-16 2008-03-25 Board Of Trustees Of The University Of Arkansas Self-contained microelectrochemical bioassay platforms and methods
US7074519B2 (en) * 2001-10-26 2006-07-11 The Regents Of The University Of California Molehole embedded 3-D crossbar architecture used in electrochemical molecular memory device
GB0130684D0 (en) * 2001-12-21 2002-02-06 Oxford Biosensors Ltd Micro-band electrode
US9740817B1 (en) 2002-10-18 2017-08-22 Dennis Sunga Fernandez Apparatus for biological sensing and alerting of pharmaco-genomic mutation
US20070042366A1 (en) * 2003-02-28 2007-02-22 Brown University Nanopores, methods for using same, methods for making same and methods for characterizing biomolecules using same
US8346482B2 (en) * 2003-08-22 2013-01-01 Fernandez Dennis S Integrated biosensor and simulation system for diagnosis and therapy
US7001717B2 (en) * 2003-12-05 2006-02-21 Biofx Laboratories, Inc. Charcoal stabilization of phenyl phosphates
US20060008581A1 (en) * 2004-07-09 2006-01-12 Mark Hyland Method of manufacturing an electrochemical sensor
CA2513340A1 (fr) * 2004-08-25 2006-02-25 F. Hoffmann-La Roche Ag Substrat reutilisable pour la production de micropuces d'adn
US20070037232A1 (en) 2005-03-31 2007-02-15 Barasch Jonathan M Detection of NGAL in chronic renal disease
US20070190542A1 (en) * 2005-10-03 2007-08-16 Ling Xinsheng S Hybridization assisted nanopore sequencing
US20080305512A1 (en) * 2006-10-26 2008-12-11 Mattingly Phillip G Assay for cardiac troponin autoantibodies
US7776605B2 (en) * 2006-10-26 2010-08-17 Abbott Laboratories Assay for cardiac troponin autoantibodies
EP2097750A2 (fr) * 2006-10-26 2009-09-09 Abbott Laboratories Analyse immunologique d'analytes dans des echantillons contenant des anticorps anti-analytes endogenes
EP2118657B1 (fr) 2006-12-29 2014-05-21 Abbott Laboratories Reactif de lyse non denaturante destine a etre utilise en immunodosage de capture en solution
US7914999B2 (en) 2006-12-29 2011-03-29 Abbott Laboratories Non-denaturing lysis reagent
US20080220537A1 (en) * 2007-03-07 2008-09-11 Pacific Biosciences Of California, Inc. Substrates and methods for selective immobilization of active molecules
WO2009046094A1 (fr) 2007-10-01 2009-04-09 Nabsys, Inc. Séquençage de biopolymère par hybridation de sondes pour former des complexes ternaires et alignement de plage variable
GB0801142D0 (en) * 2008-01-22 2008-02-27 Imp Innovations Ltd Label-free molecule detection and measurement
EP2342362B1 (fr) * 2008-09-03 2017-03-01 Nabsys 2.0 LLC Utilisation d'électrodes nanométriques longitudinalement déplacées pour une détection de tension de biomolécules et autres analytes dans des canaux fluidiques
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8455260B2 (en) * 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
US20100243449A1 (en) * 2009-03-27 2010-09-30 Oliver John S Devices and methods for analyzing biomolecules and probes bound thereto
US8246799B2 (en) * 2009-05-28 2012-08-21 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
US8835120B2 (en) 2009-12-02 2014-09-16 Abbott Laboratories Assay for cardiac troponin-T (cTnT)
US8652788B2 (en) * 2009-12-02 2014-02-18 Abbott Laboratories Assay for diagnosis of cardiac myocyte damage
US8183002B2 (en) * 2009-12-03 2012-05-22 Abbott Laboratories Autoantibody enhanced immunoassays and kits
US20110136141A1 (en) 2009-12-03 2011-06-09 Abbott Laboratories Peptide reagents and method for inhibiting autoantibody antigen binding
US8901433B2 (en) 2010-09-10 2014-12-02 Hitachi Chemical Co., Ltd. Individually addressable band electrode arrays and methods to prepare the same
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
US8859201B2 (en) 2010-11-16 2014-10-14 Nabsys, Inc. Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
WO2012109574A2 (fr) 2011-02-11 2012-08-16 Nabsys, Inc. Procédés de dosage à l'aide de protéines de liaison à l'adn
JP5355618B2 (ja) * 2011-03-10 2013-11-27 三星ディスプレイ株式會社 可撓性表示装置及びこの製造方法
EP2684048B1 (fr) 2011-03-11 2018-10-03 Board of Regents of the University of Nebraska Biomarqueur pour la maladie coronarienne
JP2014515476A (ja) 2011-05-20 2014-06-30 アボットジャパン株式会社 非特異的結合を減少させるためのイムノアッセイ方法及び試薬
GB2510752B (en) * 2011-12-28 2018-05-30 Intel Corp Nanogap transducers with selective surface immobilization sites
US9910008B2 (en) 2012-07-06 2018-03-06 Robert Bosch Gmbh Methods for generating pH/ionic concentration gradient near electrode surfaces for modulating biomolecular interactions
US9874538B2 (en) 2012-07-06 2018-01-23 Robert Bosch Gmbh Methods for generating pH/ionic concentration gradient near electrode surfaces for modulating biomolecular interactions
US9075041B2 (en) 2012-07-06 2015-07-07 Robert Bosch Gmbh Methods for generating pH/ionic concentration gradient near electrode surfaces for modulating biomolecular interactions
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
WO2014113557A1 (fr) 2013-01-18 2014-07-24 Nabsys, Inc. Liaison améliorée d'une sonde
WO2015121206A1 (fr) 2014-02-13 2015-08-20 Robert Bosch Gmbh Détection capacitive de bulles
WO2016191603A1 (fr) * 2015-05-26 2016-12-01 OncoGenesis Inc. Système, procédé et kit permettant la détection d'analytes par la production d'une espèce électrochimique
US11867660B2 (en) 2015-07-06 2024-01-09 Robert Bosch Gmbh Electronic control of the pH of a solution close to an electrode surface
US10011549B2 (en) 2015-07-06 2018-07-03 Robert Bosch Gmbh Electrochemically active agents for pH modulation in biological buffers
US10379080B2 (en) 2015-07-06 2019-08-13 Robert Bosch Gmbh Electronic control of the pH of a solution close to an electrode surfaces
JP6273315B2 (ja) * 2016-05-16 2018-01-31 インテル コーポレイション 選択的表面固定化部位を有するナノギャップ・トランスデューサ
CN106513068B (zh) * 2016-10-25 2018-10-30 清华大学 用于聚合物材质的微流控芯片键合与表面改性的溶液及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5554339A (en) * 1988-11-14 1996-09-10 I-Stat Corporation Process for the manufacture of wholly microfabricated biosensors
WO1997021094A1 (fr) * 1995-12-01 1997-06-12 Innogenetics N.V. Systeme de detection par mesure de l'impedance et procede pour le fabriquer

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8402058D0 (en) * 1984-01-26 1984-02-29 Serono Diagnostics Ltd Methods of assay
US4779031A (en) 1985-12-30 1988-10-18 Intellico, Inc. Motor system
JPH0797696B2 (ja) 1986-07-05 1995-10-18 株式会社豊田自動織機製作所 ハイブリツドｉｃ基板と回路パタ−ン形成方法
US4961806A (en) 1986-12-10 1990-10-09 Sanders Associates, Inc. Method of making a printed circuit
US4972470A (en) 1987-08-06 1990-11-20 Steven Farago Programmable connector
US5634267A (en) 1991-06-04 1997-06-03 Micron Technology, Inc. Method and apparatus for manufacturing known good semiconductor die
US5053920A (en) 1989-06-09 1991-10-01 Digital Equipment Corporation Integrated power conversion
JP2835116B2 (ja) 1989-09-29 1998-12-14 株式会社東芝 電力用ｉｃおよびその製造方法
JPH0834709B2 (ja) 1990-01-31 1996-03-29 株式会社日立製作所 半導体集積回路及びそれを使つた電動機制御装置
JP3280394B2 (ja) 1990-04-05 2002-05-13 ロックヒード マーティン コーポレーション 電子装置
JPH0513562A (ja) 1991-07-05 1993-01-22 Hitachi Ltd 駆動制御装置
US5355301A (en) 1992-02-28 1994-10-11 Fuji Electric Co., Ltd. One-chip type switching power supply device
DE4219031C2 (de) 1992-06-10 1994-11-10 Siemens Ag Multi-Chip-Modul mit Kondensator, der auf dem Träger aus Silizium (monokristalines Substrat) realisiert ist
IT1255190B (it) 1992-06-30 1995-10-20 Giuseppe Marchisi Dispositivo di interfaccia di comando per un motore elettrico
US5544017A (en) 1992-08-05 1996-08-06 Fujitsu Limited Multichip module substrate
US5384691A (en) 1993-01-08 1995-01-24 General Electric Company High density interconnect multi-chip modules including embedded distributed power supply elements
US5410107A (en) 1993-03-01 1995-04-25 The Board Of Trustees Of The University Of Arkansas Multichip module
EP0645810A4 (fr) 1993-04-06 1997-04-16 Tokuyama Corp Boitier pour puce de semiconducteur.
US5413939A (en) * 1993-06-29 1995-05-09 First Medical, Inc. Solid-phase binding assay system for interferometrically measuring analytes bound to an active receptor
JP3325351B2 (ja) 1993-08-18 2002-09-17 株式会社東芝 半導体装置
US5432675A (en) 1993-11-15 1995-07-11 Fujitsu Limited Multi-chip module having thermal contacts
JP3123343B2 (ja) 1994-05-11 2001-01-09 富士電機株式会社 安定化電源装置とその製造方法
JP3307506B2 (ja) 1994-07-13 2002-07-24 三菱電機株式会社 車両用交流発電機の制御装置および車両用交流発電機
US5434745A (en) 1994-07-26 1995-07-18 White Microelectronics Div. Of Bowmar Instrument Corp. Stacked silicon die carrier assembly
JP3230953B2 (ja) 1994-07-28 2001-11-19 富士通株式会社 多層薄膜配線基板
US5532512A (en) 1994-10-03 1996-07-02 General Electric Company Direct stacked and flip chip power semiconductor device structures
US5608617A (en) 1996-05-03 1997-03-04 Zecal Incorporated High power miniature demand power supply
US5495394A (en) 1994-12-19 1996-02-27 At&T Global Information Solutions Company Three dimensional die packaging in multi-chip modules
TW555852B (en) * 1995-03-10 2003-10-01 Meso Scale Technologies Llc Electrochemiluminescence assay apparatuses, cassettes and kits and methods of making and using the same
US5616888A (en) 1995-09-29 1997-04-01 Allen-Bradley Company, Inc. Rigid-flex circuit board having a window for an insulated mounting area
US5641944A (en) 1995-09-29 1997-06-24 Allen-Bradley Company, Inc. Power substrate with improved thermal characteristics
US5753517A (en) * 1996-03-29 1998-05-19 University Of British Columbia Quantitative immunochromatographic assays
US5958791A (en) * 1996-09-27 1999-09-28 Innovative Biotechnologies, Inc. Interdigitated electrode arrays for liposome-enhanced immunoassay and test device
US7014992B1 (en) * 1996-11-05 2006-03-21 Clinical Micro Sensors, Inc. Conductive oligomers attached to electrodes and nucleoside analogs
US6740518B1 (en) * 1998-09-17 2004-05-25 Clinical Micro Sensors, Inc. Signal detection techniques for the detection of analytes
US6485983B1 (en) * 1999-05-05 2002-11-26 Intec Science, Inc. System for electrochemical quantitative analysis of analytes within a solid phase and affinity chromatographic test strip
WO2000075792A2 (fr) * 1999-06-03 2000-12-14 Lockheed Martin Corporation Sonde d'agent biologique tres sensible

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5554339A (en) * 1988-11-14 1996-09-10 I-Stat Corporation Process for the manufacture of wholly microfabricated biosensors
WO1997021094A1 (fr) * 1995-12-01 1997-06-12 Innogenetics N.V. Systeme de detection par mesure de l'impedance et procede pour le fabriquer

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DENYER M C T ET AL: "PRELIMINARY STUDY ON THE SUITABILITY OF A PHARMACOLOGICAL BIO-ASSAYBASED ON CARDIAC MYOCYTES CULTURED OVER MICROFABRICATED MICROELECTRODE ARRAYS", MEDICAL AND BIOLOGICAL ENGINEERING AND COMPUTING, PETER PEREGRINUS LTD. STEVENAGE, GB, vol. 36, no. 5, 1 September 1998 (1998-09-01), pages 638 - 644, XP000777560, ISSN: 0140-0118 *
HINTSCHE R ET AL: "MICROBIOSENSORS USING ELECTRODES MADE IN SI-TECHNOLOGY", EXS, BIRKHAEUSER VERLAG, BASEL, CH, PAGE(S) 267-283, ISSN: 1023-294X, XP000863543 *
KONIG A ET AL: "Multimicrobial sensor using microstructured three-dimensional electrodes based on silicon technology.", ANALYTICAL CHEMISTRY, (2000 MAY 1) 72 (9) 2022-8., XP002194343 *
KOSSEK S ET AL: "IMMOBILIZATION OF STREPTAVIDIN FOR IMMUNOSENSORS ON NANOSTRUCTURED SURFACES", JOURNAL OF MOLECULAR RECOGNITION, HEYDEN & SON LTD., LONDON, GB, vol. 9, 1996, pages 485 - 487, XP000886890, ISSN: 0952-3499 *
VAN GERWEN P ET AL: "Nanoscaled interdigitated electrode arrays for biochemical sensors", 1997 INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS AND ACTUATORS. DIGEST OF TECHNICAL PAPERS. TRANSDUCERS 97. CHICAGO, IL, JUNE 16 - 19, 1997. SESSIONS 3A1 - 4D3. PAPERS NO. 3A1.01 - 4D3.14P, INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS AND ACTU, vol. 2, 16 June 1997 (1997-06-16), pages 907 - 910, XP010240622, ISBN: 0-7803-3829-4 *

Also Published As

Publication number Publication date
AU1461702A (en) 2002-04-29
US20020058279A1 (en) 2002-05-16
WO2002033410A2 (fr) 2002-04-25
EP1326712A2 (fr) 2003-07-16
US6887714B2 (en) 2005-05-03

Similar Documents

Publication Publication Date Title
WO2002033410A3 (fr) Essais d'immuno-absorbant en microvolume avec détection électrochimique amplifiée
AU1038402A (en) Assay for directly detecting a rs virus related biological cell in a body fluid sample
WO2006117161A3 (fr) Nouvelles strategies d'etiquetage pour detection sensible d'analytes
WO2005106036A3 (fr) Methodes et compositions pour analyser des analytes
WO2002077604A3 (fr) Cellules marquees servant de temoin fonctionnel interne dans des essais de detection de cellules rares
WO2003073817A3 (fr) Detection rapide et sensible de cellules et de virus
WO2006076567A3 (fr) Dispositif de detection microfluidique de cellules rares
WO2002080647A3 (fr) Methodes et reactifs de capture multiplxee d'analyte, autoassemblage de reseau de surface, et analyse d'echantillons biologiques complexes
EP1318399A3 (fr) Détection passive d'un échantillon pour commencer une analyse
WO2006078289A3 (fr) Detection d'analytes cibles fondee sur des codes a barres biologiques
WO2002079490A3 (fr) Codes-barres biologiques bases sur des particules a modification oligonucleotidique
WO2006137891A8 (fr) Detection de substances chimiques, de particules et de biocomposes faisant appel a la nanotechnologie
WO2007046825A3 (fr) Systeme de detection optoelectronique
WO2005021731A3 (fr) Detection et amplification d'analytes solubles
WO2002061129A3 (fr) Identifieurs d'oligonucleotides
EP2484781A3 (fr) Détection d'analytes et d'acides nucléiques
WO2007005666A3 (fr) Systeme et methode de detection d'analytes au moyen de recepteurs differentiels
WO2007084192A3 (fr) Dosage colorimétrique par amplification de codes-barres biochimiques pour détecter un analyte
WO2010065781A3 (fr) Dosage à bande d'écoulement latéral avec un conjugué immobilisé
WO2001040757A3 (fr) Cavites optiques resonnantes destinees a des biodetecteurs a grande sensibilite et a haut rendement et procedes utilisant ces biodetecteurs
WO2005003394A3 (fr) Detection d'analytes cible a base de codes a barres biochimiques
WO2003020878A3 (fr) Amplification et detection de reactifs pour le vih-1
WO2003050291A3 (fr) Capteurs utilises dans le test d'echantillons biologiques chimiques ou environnementaux
WO2003014697A3 (fr) Amplification de detection d'analyte au moyen de surfaces amplificatrices passivees equipees de recepteurs
SG164389A1 (en) Detection of a therapeutic antibody in an experimental animal

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2001983168

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2001983168

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: JP