WO2001081396A1 - Nouveau polypeptide, proteine ribosomale s7 humaine 14, et polynucleotide codant pour ce polypeptide - Google Patents

Nouveau polypeptide, proteine ribosomale s7 humaine 14, et polynucleotide codant pour ce polypeptide Download PDF

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Publication number
WO2001081396A1
WO2001081396A1 PCT/CN2001/000602 CN0100602W WO0181396A1 WO 2001081396 A1 WO2001081396 A1 WO 2001081396A1 CN 0100602 W CN0100602 W CN 0100602W WO 0181396 A1 WO0181396 A1 WO 0181396A1
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Prior art keywords
protein
polypeptide
polynucleotide
human
sequence
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PCT/CN2001/000602
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English (en)
Chinese (zh)
Inventor
Yumin Mao
Yi Xie
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Biowindow Gene Development Inc. Shanghai
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Application filed by Biowindow Gene Development Inc. Shanghai filed Critical Biowindow Gene Development Inc. Shanghai
Priority to AU76231/01A priority Critical patent/AU7623101A/en
Publication of WO2001081396A1 publication Critical patent/WO2001081396A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Definitions

  • the present invention belongs to the field of biotechnology. Specifically, the present invention describes a new polypeptide, a human ribosome S 7 protein 14, and a polynucleotide sequence encoding the polypeptide. The invention also relates to a preparation method and application of the polynucleotide and polypeptide. Background technique
  • the ribosome size subunits are often free in the cytoplasmic matrix within the cell. Only when the small subunits are combined with mRNA do the large subunits bind to the small subunits to form a complete ribosome. After the peptide synthesis is terminated, the large and small subunits dissociate and then exist freely in the cytoplasmic matrix.
  • the invention also relates to a method for screening compounds that mimic, activate, antagonize or inhibit the activity of human ribosomal S 7 protein 14 protein, which comprises using the polypeptide of the invention.
  • the invention also relates to compounds obtained by this method.
  • this polypeptide has a similar expression profile to human ribosomal S 7 protein 9, and it can be deduced that the human ribosome S7 protein 14 has similar functions to human ribosomal S 7 protein 9.
  • genomic DNA isolation is the least commonly used. Direct chemical synthesis of DNA sequences is often the method of choice. The more commonly used method is the isolation of cDNA sequences.
  • the standard method for isolating cDNA of interest is to isolate mRNA from donor cells that overexpress the gene and perform reverse transcription to form a plasmid or phage cDNA library. There are many mature techniques for extracting mRNA, and kits are also commercially available (Qiagene).
  • the construction of cDNA libraries is also a common method (Sambrook, et al., Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory. New York, 1989).
  • Commercially available cDNA libraries are also available, such as different cDNA libraries from Clontech. When polymerase reaction technology is used in combination, even very small expression products can be cloned.
  • polynucleotide sequence of the present invention can be used to express or produce recombinant human ribosome S7 protein 14 (Science, 1984; 224: 1431). Generally there are the following steps:
  • 1 indicates fetal kidney
  • 2 indicates fetal large intestine
  • 3 indicates fetal small intestine
  • 4 indicates fetal muscle
  • 5 indicates fetal brain
  • 6 indicates fetal bladder
  • 7 indicates non-starved L02
  • 8 indicates L02 +, lhr, As 3+
  • 9 indicates ECV304 PMA-
  • 10 means ECV304 PMA +
  • 11 means fetal liver
  • 12 means normal liver
  • 13 means thyroid
  • I 4 means skin
  • 15 means fetal lung
  • 16 means lung
  • 17 means lung cancer
  • 18 means fetal spleen
  • 19 means spleen
  • 20 is the prostate
  • 21 is the fetal heart
  • 22 is the heart
  • 23 is the muscle
  • 24 is the testis
  • 25 is the fetal thymus
  • 26 is the thymus.
  • the determined cDNA sequence was compared with the existing public DNA sequence database (Genebank), and the cDM sequence of one of the clones 0212g08 was found to be new DNA.
  • the inserted cDNA fragment contained in this clone was determined in both directions by synthesizing a series of primers.
  • the results show that the full-length cDNA contained in the 0212g08 clone is 1328bp (as shown in Seq ID NO: 1), and there is a 374bp open reading frame (0RF) from 16bp to 390bp, which encodes a new protein (such as Seq ID NO : Shown in 2).
  • This clone pBS-0212g08 and the encoded protein was named human ribosome S7 protein 14.
  • Example 2 Cloning of a gene encoding human ribosomal S 7 protein 14 by RT-PCR
  • Primer 3 5'-CCCCATATGATGATGATCATTTATAGGAGAAAA-3 '(Seq ID No: 5)
  • peptides specific to human ribosomal S7 protein 14 were synthesized using a peptide synthesizer (product of PE company): NH2-Met-Met-Ile-Ile-Tyr-Arg-Arg-Lys-Lys-Gly-I le-Gly- Cys-Lys-Gly- C00H (SEQ ID NO: 7).
  • the polypeptide was coupled to hemocyanin and bovine serum albumin to form a complex, respectively. For the method, see: Avrameas, et a 1. I unochemistry, 1969; 6: 43.
  • oligonucleotide fragments for use as hybridization probes from the polynucleotide SEQ ID NO: 1 of the present invention should follow the following principles and several aspects to be considered:
  • PBS phosphate buffered saline
  • the abnormal expression of the human ribosome S7 protein 14 of the present invention will produce various diseases, especially embryonic developmental disorders, growth and development disorders, various tumors, and inflammations. These diseases include, but are not limited to:
  • Tumors of various tissues gastric cancer, liver cancer, lung cancer, esophageal cancer, breast cancer, leukemia, lymphoma, thyroid tumor, uterine fibroids, neuroblastoma, astrocytoma, ependymoma, glioblastoma, Colon cancer, malignant histiocytosis, bladder cancer, bone cancer, osteosarcoma, myeloma, bone marrow cancer, brain cancer, Uterine cancer, endometrial cancer, gallbladder cancer, colon cancer, thymic tumor, nasal cavity and sinus tumor, nasopharyngeal cancer, laryngeal cancer, tracheal tumor, pleural mesothelioma, fibroma, fibrosarcoma, lipoma, liposarcoma, leiomyoma Myoma
  • PCR primers (preferably 15-35bp) are prepared based on cDNA, and the sequences can be located on chromosomes. These primers were then used for PCR screening of somatic hybrid cells containing individual human chromosomes. Only those heterozygous cells containing the human gene corresponding to the primer will produce amplified fragments.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un nouveau polypeptide, une protéine ribosomale S7 humaine 14, et un polynucléotide codant pour ce polypeptide ainsi qu'un procédé d'obtention de ce polypeptide par des techniques recombinantes d'ADN. L'invention concerne en outre les applications de ce polypeptide dans le traitement de maladies, notamment des tumeurs malignes, de l'hémopathie, de l'infection par VIH, de maladies immunitaires et de diverses inflammations. L'invention concerne aussi l'antagoniste agissant contre le polypeptide et son action thérapeutique ainsi que les applications de ce polynucléotide codant pour la protéine ribosomale S7 humaine 14.
PCT/CN2001/000602 2000-04-27 2001-04-23 Nouveau polypeptide, proteine ribosomale s7 humaine 14, et polynucleotide codant pour ce polypeptide WO2001081396A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU76231/01A AU7623101A (en) 2000-04-27 2001-04-23 A new polypeptide - human ribosomal s7 protein 14 and the polynucleotide encoding it

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN00115450.8 2000-04-27
CN00115450A CN1320613A (zh) 2000-04-27 2000-04-27 一种新的多肽——人核糖体s7蛋白14和编码这种多肽的多核苷酸

Publications (1)

Publication Number Publication Date
WO2001081396A1 true WO2001081396A1 (fr) 2001-11-01

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PCT/CN2001/000602 WO2001081396A1 (fr) 2000-04-27 2001-04-23 Nouveau polypeptide, proteine ribosomale s7 humaine 14, et polynucleotide codant pour ce polypeptide

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CN (1) CN1320613A (fr)
AU (1) AU7623101A (fr)
WO (1) WO2001081396A1 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4865970A (en) * 1986-02-28 1989-09-12 Hoffmann-La Roche Inc. Method of detecting ribosomal protein antibodies in systemic lupus erythematosus
WO2000028079A2 (fr) * 1998-11-09 2000-05-18 Gemini Genomics Ab Variation genetique associee a l'anemie aplasique, et applications diagnostiques et therapeutiques basees sur cette variation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4865970A (en) * 1986-02-28 1989-09-12 Hoffmann-La Roche Inc. Method of detecting ribosomal protein antibodies in systemic lupus erythematosus
WO2000028079A2 (fr) * 1998-11-09 2000-05-18 Gemini Genomics Ab Variation genetique associee a l'anemie aplasique, et applications diagnostiques et therapeutiques basees sur cette variation

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK [online] 24 July 1998 (1998-07-24), BIRREN B. ET AL.: "Homo sapiens chromosome 17, clone hRPC.1081_P_3", Database accession no. AC005207 *
DECKERT G. ET AL.: "The complete genome of the hyperthermophilic bacterium aquifex aeolicus", NATURE, vol. 392, no. 6674, 1998, pages 353 - 358 *
IVENS A.C. ET AL.: "A physical map of the leishmania major Friedlin genome", GENOME RESEARCH, vol. 8, no. 2, 1998, pages 135 - 145 *
KIKUNO R. ET AL.: "Prediction of the coding sequences of unidentified human genes XIV. The complete sequences of 100 new cDNA clones from brain which code for large protein in vitro", DNA RES., vol. 6, no. 3, 1999, pages 197 - 205, XP000852618 *

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Publication number Publication date
CN1320613A (zh) 2001-11-07
AU7623101A (en) 2001-11-07

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