WO2001079537A3 - Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung - Google Patents

Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung Download PDF

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Publication number
WO2001079537A3
WO2001079537A3 PCT/EP2001/002234 EP0102234W WO0179537A3 WO 2001079537 A3 WO2001079537 A3 WO 2001079537A3 EP 0102234 W EP0102234 W EP 0102234W WO 0179537 A3 WO0179537 A3 WO 0179537A3
Authority
WO
WIPO (PCT)
Prior art keywords
detecting
test system
splicing reaction
intron
splicing
Prior art date
Application number
PCT/EP2001/002234
Other languages
English (en)
French (fr)
Other versions
WO2001079537A2 (de
Inventor
Bettina Bauer
Eberhard Schneider
Marion Bartel
Peter Wagner
Original Assignee
Aventis Res & Tech Gmbh & Co
Bettina Bauer
Eberhard Schneider
Marion Bartel
Peter Wagner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Res & Tech Gmbh & Co, Bettina Bauer, Eberhard Schneider, Marion Bartel, Peter Wagner filed Critical Aventis Res & Tech Gmbh & Co
Priority to EP01929371A priority Critical patent/EP1276908A2/de
Priority to AU2001256170A priority patent/AU2001256170A1/en
Priority to US10/257,554 priority patent/US20040038221A1/en
Publication of WO2001079537A2 publication Critical patent/WO2001079537A2/de
Publication of WO2001079537A3 publication Critical patent/WO2001079537A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein homogenes Testsystem enthaltend (a) mindestens eine oder mehrere, gleiche oder verschiedene mobile Nukleinsäuren mit mindestens einer spleissfähigen Nukleinsäure, enthaltend mindestens ein Intron, (b) mindestens zwei Sonden, enthaltend korrespondierende Fluorophore, welche vorzugsweise an den 5' und/oder 3' Enden eines Exon/Intron binden, (c) mindestens ein gelfreies Nachweissystem zum Nachweis einer Spleissreaktion, gegebenenfalls (d) mindestens eine Zusammensetzung enthaltend Spleisskomponenten, (e) weitere Hilfsmittel.
PCT/EP2001/002234 2000-04-14 2001-02-28 Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung WO2001079537A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01929371A EP1276908A2 (de) 2000-04-14 2001-02-28 Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung
AU2001256170A AU2001256170A1 (en) 2000-04-14 2001-02-28 Test system for detecting a splicing reaction and use thereof
US10/257,554 US20040038221A1 (en) 2000-04-14 2001-02-28 Test system for detecting a splicing reaction and use therof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10018465A DE10018465A1 (de) 2000-04-14 2000-04-14 Testsystem zum Nachweis einer Spleißreaktion, sowie dessen Verwendung
DE10018465.0 2000-04-14

Publications (2)

Publication Number Publication Date
WO2001079537A2 WO2001079537A2 (de) 2001-10-25
WO2001079537A3 true WO2001079537A3 (de) 2002-11-28

Family

ID=7638698

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/002234 WO2001079537A2 (de) 2000-04-14 2001-02-28 Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung

Country Status (5)

Country Link
US (1) US20040038221A1 (de)
EP (1) EP1276908A2 (de)
AU (1) AU2001256170A1 (de)
DE (1) DE10018465A1 (de)
WO (1) WO2001079537A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007031574B4 (de) 2007-07-06 2012-01-26 Karlsruher Institut für Technologie Minor-Spleißosom Testsystem zur Modulierung der Zellteilung

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992014841A1 (en) * 1991-02-14 1992-09-03 Baxter Diagnostics Inc. Novel lanthanide chelate-conjugated oligonucleotides
WO1997046714A1 (en) * 1996-06-04 1997-12-11 University Of Utah Research Foundation Monitoring hybridization during pcr
DE19909156A1 (de) * 1999-03-02 2000-09-07 Aventis Res & Tech Gmbh & Co Testsystem zum Nachweis einer Spleißreaktion, sowie dessen Verwendung

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992014841A1 (en) * 1991-02-14 1992-09-03 Baxter Diagnostics Inc. Novel lanthanide chelate-conjugated oligonucleotides
WO1997046714A1 (en) * 1996-06-04 1997-12-11 University Of Utah Research Foundation Monitoring hybridization during pcr
DE19909156A1 (de) * 1999-03-02 2000-09-07 Aventis Res & Tech Gmbh & Co Testsystem zum Nachweis einer Spleißreaktion, sowie dessen Verwendung

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AUSUBEL F M: "Preparation of protein extracts from yeast. In: Current protocols in molecular biology", 1994, JOHN WILEY & SONS, CANADA, XP002207202 *
ERSTER S H ET A.: "Use of RNase H and primer extension to analyze RNA splicing", NUCLEIC ACIDS RESEARCH, vol. 16, no. 13, 1988, pages 5999 - 6014, XP002207201 *
GUTHRIE C: "Messenger RNA splicing in yeast: Clues to why the spliceosome is a ribonucleoprotein", SCIENCE, vol. 253, 1991, pages 157, XP002207199 *
MORGAN W R ET AL: "THREE SPLICING PATTERNS ARE USED TO EXCISE THE SMALL INTRON COMMON TO ALL MINUTE VIRUS OF MICE RNAS", JOURNAL OF VIROLOGY, NEW YORK, US, US, vol. 60, no. 3, October 1986 (1986-10-01), pages 1170 - 1174, XP000961665, ISSN: 0022-538X *
VOLLOCH V ET AL: "INHIBITION OF PRE-MRNA SPLICING BY ANTISENSE RNA IN VITRO: EFFECT OF RNA CONTAINING SEQUENCES COMPLEMENTARY TO EXONS", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 179, no. 3, 30 September 1991 (1991-09-30), pages 1593 - 1599, XP001053533, ISSN: 0006-291X *
ZANELLI E ET AL.: "Evidence for an alternate splicing in the thyroperoxidase messenger from patients with Grave's disease", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 170, no. 2, 1990, pages 735 - 741, XP002207200 *

Also Published As

Publication number Publication date
DE10018465A1 (de) 2001-10-18
EP1276908A2 (de) 2003-01-22
US20040038221A1 (en) 2004-02-26
AU2001256170A1 (en) 2001-10-30
WO2001079537A2 (de) 2001-10-25

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