WO2001079537A3 - Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung - Google Patents

Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung Download PDF

Info

Publication number
WO2001079537A3
WO2001079537A3 PCT/EP2001/002234 EP0102234W WO0179537A3 WO 2001079537 A3 WO2001079537 A3 WO 2001079537A3 EP 0102234 W EP0102234 W EP 0102234W WO 0179537 A3 WO0179537 A3 WO 0179537A3
Authority
WO
WIPO (PCT)
Prior art keywords
detecting
test system
splicing reaction
intron
splicing
Prior art date
Application number
PCT/EP2001/002234
Other languages
English (en)
French (fr)
Other versions
WO2001079537A2 (de
Inventor
Bettina Bauer
Eberhard Schneider
Marion Bartel
Peter Wagner
Original Assignee
Aventis Res & Tech Gmbh & Co
Bettina Bauer
Eberhard Schneider
Marion Bartel
Peter Wagner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Res & Tech Gmbh & Co, Bettina Bauer, Eberhard Schneider, Marion Bartel, Peter Wagner filed Critical Aventis Res & Tech Gmbh & Co
Priority to US10/257,554 priority Critical patent/US20040038221A1/en
Priority to EP01929371A priority patent/EP1276908A2/de
Priority to AU2001256170A priority patent/AU2001256170A1/en
Publication of WO2001079537A2 publication Critical patent/WO2001079537A2/de
Publication of WO2001079537A3 publication Critical patent/WO2001079537A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein homogenes Testsystem enthaltend (a) mindestens eine oder mehrere, gleiche oder verschiedene mobile Nukleinsäuren mit mindestens einer spleissfähigen Nukleinsäure, enthaltend mindestens ein Intron, (b) mindestens zwei Sonden, enthaltend korrespondierende Fluorophore, welche vorzugsweise an den 5' und/oder 3' Enden eines Exon/Intron binden, (c) mindestens ein gelfreies Nachweissystem zum Nachweis einer Spleissreaktion, gegebenenfalls (d) mindestens eine Zusammensetzung enthaltend Spleisskomponenten, (e) weitere Hilfsmittel.
PCT/EP2001/002234 2000-04-14 2001-02-28 Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung WO2001079537A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/257,554 US20040038221A1 (en) 2000-04-14 2001-02-28 Test system for detecting a splicing reaction and use therof
EP01929371A EP1276908A2 (de) 2000-04-14 2001-02-28 Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung
AU2001256170A AU2001256170A1 (en) 2000-04-14 2001-02-28 Test system for detecting a splicing reaction and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10018465.0 2000-04-14
DE10018465A DE10018465A1 (de) 2000-04-14 2000-04-14 Testsystem zum Nachweis einer Spleißreaktion, sowie dessen Verwendung

Publications (2)

Publication Number Publication Date
WO2001079537A2 WO2001079537A2 (de) 2001-10-25
WO2001079537A3 true WO2001079537A3 (de) 2002-11-28

Family

ID=7638698

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/002234 WO2001079537A2 (de) 2000-04-14 2001-02-28 Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung

Country Status (5)

Country Link
US (1) US20040038221A1 (de)
EP (1) EP1276908A2 (de)
AU (1) AU2001256170A1 (de)
DE (1) DE10018465A1 (de)
WO (1) WO2001079537A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007031574B4 (de) 2007-07-06 2012-01-26 Karlsruher Institut für Technologie Minor-Spleißosom Testsystem zur Modulierung der Zellteilung

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992014841A1 (en) * 1991-02-14 1992-09-03 Baxter Diagnostics Inc. Novel lanthanide chelate-conjugated oligonucleotides
WO1997046714A1 (en) * 1996-06-04 1997-12-11 University Of Utah Research Foundation Monitoring hybridization during pcr
DE19909156A1 (de) * 1999-03-02 2000-09-07 Aventis Res & Tech Gmbh & Co Testsystem zum Nachweis einer Spleißreaktion, sowie dessen Verwendung

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992014841A1 (en) * 1991-02-14 1992-09-03 Baxter Diagnostics Inc. Novel lanthanide chelate-conjugated oligonucleotides
WO1997046714A1 (en) * 1996-06-04 1997-12-11 University Of Utah Research Foundation Monitoring hybridization during pcr
DE19909156A1 (de) * 1999-03-02 2000-09-07 Aventis Res & Tech Gmbh & Co Testsystem zum Nachweis einer Spleißreaktion, sowie dessen Verwendung

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AUSUBEL F M: "Preparation of protein extracts from yeast. In: Current protocols in molecular biology", 1994, JOHN WILEY & SONS, CANADA, XP002207202 *
ERSTER S H ET A.: "Use of RNase H and primer extension to analyze RNA splicing", NUCLEIC ACIDS RESEARCH, vol. 16, no. 13, 1988, pages 5999 - 6014, XP002207201 *
GUTHRIE C: "Messenger RNA splicing in yeast: Clues to why the spliceosome is a ribonucleoprotein", SCIENCE, vol. 253, 1991, pages 157, XP002207199 *
MORGAN W R ET AL: "THREE SPLICING PATTERNS ARE USED TO EXCISE THE SMALL INTRON COMMON TO ALL MINUTE VIRUS OF MICE RNAS", JOURNAL OF VIROLOGY, NEW YORK, US, US, vol. 60, no. 3, October 1986 (1986-10-01), pages 1170 - 1174, XP000961665, ISSN: 0022-538X *
VOLLOCH V ET AL: "INHIBITION OF PRE-MRNA SPLICING BY ANTISENSE RNA IN VITRO: EFFECT OF RNA CONTAINING SEQUENCES COMPLEMENTARY TO EXONS", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 179, no. 3, 30 September 1991 (1991-09-30), pages 1593 - 1599, XP001053533, ISSN: 0006-291X *
ZANELLI E ET AL.: "Evidence for an alternate splicing in the thyroperoxidase messenger from patients with Grave's disease", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 170, no. 2, 1990, pages 735 - 741, XP002207200 *

Also Published As

Publication number Publication date
WO2001079537A2 (de) 2001-10-25
US20040038221A1 (en) 2004-02-26
DE10018465A1 (de) 2001-10-18
EP1276908A2 (de) 2003-01-22
AU2001256170A1 (en) 2001-10-30

Similar Documents

Publication Publication Date Title
DE69901714D1 (de) Elektrochemischer affinitätsassay
WO2002034946A3 (en) Cotton event pv-ghgt07(1445) and compositions and methods for detection thereof
WO2002020569A3 (en) Mammalian genes; related reagents and methods
DE69921199D1 (de) Zweirichtungsquerflussteststreifen und verfahren
BR9811039A (pt) Citocina de mamìferos: interleucina-b30 e reagentes relacionados
DE69730820D1 (de) Polyazomacrocyclofluoromonoalkylphosphensäure, ihre komplexe zur verwendung als kontrastmittel
ATE278186T1 (de) Elektrochemischer sensor zur bestimmung der blutgerinnung, ein entsprechendes blutgerinnungsmesssystem sowie ein verfahren zur bestimmung der blutgerinnung
DE69828827D1 (de) Verbessertes Prüfsystem für Proben
WO2002036831A3 (en) Canola event pv-bngt04(rt73) and compositions and methods for detection thereof
DE69918590D1 (de) Substituierte Karbazole, Verfahren zu ihrer Herstellung und ihre Verwendung als sPLA2-Inhibitoren
SE0000538D0 (sv) Methods and kits for proximity probing
WO2000046407A3 (en) Methods of utilizing the tt virus
DE60030145D1 (de) Nachweisverfahren für kurze sequenzvarianten
DE69609161D1 (de) Amplifikationsoligonukleotide und sonden für borrelia, die mit lyme kranheit assoziiert sind
DE69501228D1 (de) Ascorbatoxidase, dafür kodierendes Gen, Verfahren zu ihrer Herstellung und dieselbe verwendende Reagens-Zusammensetzung
FI953990A (fi) Menetelmä nukleiinihappomonistusreaktiotuotteen tekemiseksi kykenemät-tömäksi olemaan lisämonistuksen kohteena, diagnostinen määritysmenetelmä, jossa tätä menetelmää käytetään, ja menetelmän tai määrityksen toteutukseen sopiva testipakkaus ja säiliö
DE50210166D1 (de) Selektive Farbveränderung keratinischer Fasern
CA2339821A1 (en) Enzymatic measurement of mycophenolic acid
WO2001079537A3 (de) Testsystem zum nachweis einer spleissreaktion, sowie dessen verwendung
DE69913325D1 (de) Nephrin gen und protein
DE69804047D1 (de) Drehmomentprüfer für Lager
EP1180549A4 (de) Verfahren zum messen von polymorphismen
EP1030931A4 (de) Harnsäure-versuchsanordnung mit stabilisiertem urikasereagenz
CA2131719A1 (en) Nucleic acid sequences coding for or complementary to nucleic acid sequences coding for interleukin 9 receptor
WO1998026097A3 (en) NUCLEIC ACID PRIMERS AND PROBES FOR DETECTING $i(LEGIONELLA PNEUMOPHILA)

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWE Wipo information: entry into national phase

Ref document number: 2001929371

Country of ref document: EP

AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWP Wipo information: published in national office

Ref document number: 2001929371

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 10257554

Country of ref document: US

WWW Wipo information: withdrawn in national office

Ref document number: 2001929371

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP