WO2001074990A1 - Procede pour cultiver des algues - Google Patents

Procede pour cultiver des algues Download PDF

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Publication number
WO2001074990A1
WO2001074990A1 PCT/NL2001/000273 NL0100273W WO0174990A1 WO 2001074990 A1 WO2001074990 A1 WO 2001074990A1 NL 0100273 W NL0100273 W NL 0100273W WO 0174990 A1 WO0174990 A1 WO 0174990A1
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WO
WIPO (PCT)
Prior art keywords
process according
algae
cultured
organisms
micro
Prior art date
Application number
PCT/NL2001/000273
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English (en)
Inventor
Johannes Henricus Reith
Cornelis Johannes Josef Marinus Hack
Ronald Kalwij
Lucas Roelof Mur
Original Assignee
Stichting Energieonderzoek Centrum Nederland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stichting Energieonderzoek Centrum Nederland filed Critical Stichting Energieonderzoek Centrum Nederland
Priority to AU2001246949A priority Critical patent/AU2001246949A1/en
Priority to EP01920003A priority patent/EP1272607A1/fr
Publication of WO2001074990A1 publication Critical patent/WO2001074990A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/18—Open ponds; Greenhouse type or underground installations
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel

Definitions

  • the invention relates to a process of culturing photosynthetic micro-organisms
  • Algae cultivation is an environmentally friendly and energetically efficient process for the production of organic material by photosynthesis from carbon dioxide and luminous energy.
  • use is made of gratis energy from sunlight, gratis carbon dioxide and water, which can be of low quality, including industrial process water, effluent of biological water treatment or other waste water streams.
  • Products of algae cultivation include algal biomass and purified water which can be used, for example, as industrial water. If the carbon dioxide stems from flue gas, this algae production also contributes to flue gas cleaning, not least because nitrogen compounds (NOx) too can be removed from the flue gas by the algae.
  • NOx nitrogen compounds
  • the algal biomass produced can - depending on the cultured species - be used in the extraction of a series of high-value-added substances such as: fatty acids (including polyunsaturated fatty acids), pigments, polysaccharides, and a number of other biologically active substances. These products can be used in nutrition and nutritional supplements, cosmetics and other "personal care" products and in clinical and pharmaceutical products.
  • the remainder of the biomass can be designated as animal feed, fertiliser or as a raw material for energy production. Integration of production functions and environmental functions is a beneficial feature of algae cultivation. Culturing algae requires the input of (sun)light (as an energy source for photosynthesis) and a sufficient supply of nutrients in dissolved form in the culture medium.
  • these are: carbon in the form of CO 2 , HCO " or CO 2 derived from mineralisation of organic substances in the feed water, a nitrogen source (generally NO 3 " , NH 4 + or urea), phosphate and a number of other nutrients including sulphur, potassium, magnesium and trace elements.
  • a nitrogen source generally NO 3 " , NH 4 + or urea
  • phosphate and a number of other nutrients including sulphur, potassium, magnesium and trace elements.
  • this organic substance in the algae system is mineralised by bacteria, thus making CO 2 available for uptake by the algae.
  • a number of photosynthetic microorganisms are capable of taking up organic substances directly and using them as a nutrient and an energy source, in combination with the photosynthetic process.
  • "clean" culturing is also possible by making use of, for example, surface water or tap water, to which the nutrients are added in the form of e.g. artificial fertiliser products and pure, technical-grade CO 2 .
  • the open systems in order to increase their efficiency, are generally designed as a continuous culture in which a fixed supply of culture medium or influent ensures constant dilution of the system.
  • the organisms adapt their growth rate to this dilution regime, the organism best adapted to the environment prevailing in the system winning the competition with the other organisms.
  • a drawback of the common open algae culture systems is the major risk of infection by undesirable photosynthetic micro-organisms which can be introduced via air or rain.
  • undesirable photosynthetic micro-organisms which can be introduced via air or rain.
  • the composition and quality of the biomass produced (and thus the yield of the desired product) cannot be controlled.
  • Such infections can be prevented only by choosing a culture medium which is unfavourable for infectious and other undesirable micro-organisms and favourable to growth of the desired alga species, so that the latter can win the competition. In a limited number of cases this is possible.
  • a high pH and high alkalinity are selective for this alga species.
  • Chlorella species such as C. pyrenoidosa and C. vulgaris
  • Dunaliella species inter alia Dunaliella salina
  • the selective advantages which make this possible for these groups of algae are, respectively: the high growth rate which allows the competition from other organisms to be won (in the case of Chlorella sp.) and the salt water environment (in the case of Dunaliella sp.).
  • An alternative to the outlined problems could be to carry out algae cultivation in closed photobioreactors.
  • the process conditions can be accurately controlled, and no infections carrying undesirably alga species will occur.
  • a major drawback of the closed photobioreactors resides in the high investment costs which lead to high production costs.
  • the technology of the photobioreactors is as yet not sufficiently developed for large-scale application.
  • the process of culturing algae according to the invention is based on the use of a series of open reactors connected in series.
  • the total system behaves as a plug-flow reactor, increasingly so as the number of connected reactors increases.
  • This plug-flow reactor is inoculated, preferably continuously or alternatively periodically, with a large quantity of algae which have been precultured under controlled conditions in a closed photobioreactor and/or some other culture system sealed off from the outside air or adequately covered (e.g. a greenhouse).
  • a closed reactor here therefore refers to a reactor which is adequately sealed from possible sources of infection; depending on circumstances, this can also be a semi-closed reactor.
  • the large quantity of algae with which the plug-flow reactor is inoculated from the closed preculture is at least 10 5 cells per charge; on the other hand, this quantity can be expressed in terms of the ultimately recovered quantity of algae or photosynthetic bacteria, preferably at least 100 ppm of that ultimate quantity.
  • a characteristic of the reactor system of the plug-flow type is that no macro-back- mixing takes place, thus ensuring that any contamination which may occur cannot develop to higher densities and will be flushed out.
  • the underlying principle of bulk inoculation with a controlled preculture is illustrated within the following frame.
  • the biomass after a period t can be calculated by the following equation
  • the biomass formed after a period t is therefore defined by N 0 and the generation time T
  • N 240 1.024 . 10 9 cells
  • An infection with species B which enters at the start of the system and has a generation time which is half that of organism A will, after 240 hours, have a biomass of:
  • N 240 1*049 . 10 6 cells
  • a open system based on bulk inoculation and a constant stream in one direction can be used to produce relatively slow-growing alga species without problems with aerial infections. In principle, it is thus possible to culture any alga species desired.
  • the series of connected open reactors numbers at least three reactors, preferably at least four reactors.
  • the connection is such that as the contents of the one reactor are transferred to the next reactor, not more than 5% backmixing takes place, i.e. that not more than 5% of the contents of the reactor which are passed on to the downstream reactor are mixed with the next contents, from the upstream reactor.
  • this percentage is lower, for example at most 2% or rather at most 1%. It will be evident that the lower this backmixing percentage, the smaller the series of reactors which is sufficient with regard to restricting infections.
  • a conceivable design of such a system involves a cascade of connected tubular or trough reactors (troughs, panels, tubes or comparable) or a cascade of ideal stirred reactor systems (CISRTs).
  • the latter can be either ideal stirred tank reactors or tubular reactors comprising static mixers or equivalent systems.
  • an exponential increase in the biomass takes place (as explained within the frame above).
  • This optimum density is a function of the incident light intensity and the depth of the culture.
  • the elements of the system will preferably show an exponential increase in surface area.
  • the increase in surface area can be obtained by an increase in the number of elements in the downstream direction. Instead, an increase in surface area can be obtained by an increase in the reactor volume in the downstream direction.
  • the volume increase is achieved by additional culture medium or waste water containing nutrients being fed in at two or more locations, thereby maintaining the algae concentration at a constant level. This admixture is quantified in terms of the algae concentration and consequently of the growth rate of the algae in the system. Consequently, the flow velocity is likewise a function of the instantaneous growth rate of the cultured alga.
  • the algae are separated from the culture medium, thereby affording as products: purified water and algal biomass as a raw material for the extraction of products.
  • Figure 1 illustrates the principle with reference to a system consisting of a closed tubular bioreactor for continuous inoculation of the open system section, which - in this example - consists of open troughs.
  • the system is suitable, in principle, for large-scale culture of all desired species and strains of photosynthetic micro-organisms, including those species and strains which in the present state of the art can be cultured successfully only in closed reactor systems, because of the above-described contamination problems.
  • This ensures that - by virtue of the "free" choice of species and/or strains - it is also possible to make a "free” choice of the desired products from the range of components which are produced by photosynthetic micro-organisms, and that considerably lower production costs per unit of biomass and per unit of product are achieved, as the system found requires considerably lower investment than closed bioreactor systems on a comparable scale.
  • the process and the system according to the invention are suitable for any species or strain from the group of photosynthetic micro-organisms.
  • This comprises photosynthetic bacteria and micro-algae from the order Cyanobacteria (formerly sometimes also referred to as algae order Cyanophyta), the order Chlorophyta (green algae), the order Chromophyta, the order Cryptophyta, the order Pyrrhophyta (dinoflagellates), the order Euglenophyta and the order Rhodophyta (red micro-algae).
  • the Chromophyta inter alia include the classes Bacillariophyceae (diatoms), Chrysophyceae (golden algae), Eustigmatophyceae and Xanthophyceae (yellow-green algae). According to older classifications, some of these classes form orders of their own (Bacillariophyta, Xanthophyta and the like).
  • Examples are the culture of Monodus species (Eustimatophyceae) for the production of polyunsaturated fatty acids. To be mentioned among these is the species Monodus subterraneus, a freshwater species having an optimum culture temperature of about 25°C and an optimum growth rate ( ⁇ ) of about 0.04 h "1 .
  • Other examples are species and strains from the micro-algae genera Porphyridium sp. (Rhodophyta) for the production of phycobiliproteins and Chlorella sp. (Chlorophyta) for the production of carotenoids and bioactive substances, and the cyanobacteria genera Nostoc sp. , (for the production of phycobiliproteins) and Calothrix sp. (for phycobiliproteins and byproducts).
  • FIG 1 gives an overview of an integral culture system according to the invention.
  • FIG. 2 is a schematic depiction of the principle of a combination of a closed photobioreactor for producing the inoculum (A) and the open system section, the Multiplier (B) in which the algae reside for a number of generations (in this example 4 generations).
  • the bottom section of the figure shows the principle of the open system section in which the surface area increases exponentially, as indicated by the exponential increase in the number of boxes.
  • these boxes will consist either of (connected) tubular or trough reactors (troughs, channels, tubes or the like) or (connected) ideal stirred reactor systems (CISTRs).
  • these individual elements of the system will preferably have a larger surface area than the boxes as drawn, on within the condition that the surface area of the system as a whole increases exponentially in the direction of the plug flow.

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Selon l'invention, des algues et d'autres micro-organismes à effet photosynthétique peuvent être cultivés de manière opportune dans un système ouvert, par inoculation d'une substance aqueuse avec une ou plusieurs espèces ou souches dudit groupe de micro-organismes, par mise en culture desdits micro-organismes et par récupération de la biomasse produite. Selon l'invention, les micro-organismes à effet photosynthétique devraient être mis en culture dans une série de réacteurs ouverts dont le premier est inoculé avec une grande quantité d'inoculum mis en culture préalable dans un photobioréacteur fermé.
PCT/NL2001/000273 2000-04-03 2001-04-03 Procede pour cultiver des algues WO2001074990A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2001246949A AU2001246949A1 (en) 2000-04-03 2001-04-03 Process of culturing algae
EP01920003A EP1272607A1 (fr) 2000-04-03 2001-04-03 Procede pour cultiver des algues

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL1014825A NL1014825C2 (nl) 2000-04-03 2000-04-03 Werkwijze voor het kweken van algen.
NL1014825 2000-04-03

Publications (1)

Publication Number Publication Date
WO2001074990A1 true WO2001074990A1 (fr) 2001-10-11

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PCT/NL2001/000273 WO2001074990A1 (fr) 2000-04-03 2001-04-03 Procede pour cultiver des algues

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EP (1) EP1272607A1 (fr)
AU (1) AU2001246949A1 (fr)
NL (1) NL1014825C2 (fr)
WO (1) WO2001074990A1 (fr)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6509188B1 (en) * 1999-04-13 2003-01-21 Fraunhofer-Gesellschaft Zur Photobioreactor with improved supply of light by surface enlargement, wavelength shifter bars or light transport
ES2193845A1 (es) * 2001-07-16 2003-11-01 Seaweed Canarias S L Procedimiento para la obtencion de un multiextracto para aplicacion en agroecosistemas.
WO2007013899A2 (fr) * 2005-06-07 2007-02-01 Hr Biopetroleum, Inc. Procede hybride continu de production d'huile et autres produits utiles a partir de microbes photosynthetiques
WO2009077087A1 (fr) * 2007-12-14 2009-06-25 Eni S.P.A. Procédé de production d'une biomasse algale ayant une teneur lipidique élevée
US7980024B2 (en) 2007-04-27 2011-07-19 Algae Systems, Inc. Photobioreactor systems positioned on bodies of water
WO2011113974A1 (fr) * 2010-03-17 2011-09-22 Universidad De Alicante Système de réacteur ouvert pour la culture de micro-algues
US8110395B2 (en) 2006-07-10 2012-02-07 Algae Systems, LLC Photobioreactor systems and methods for treating CO2-enriched gas and producing biomass
EP2584884A1 (fr) * 2010-06-23 2013-05-01 General Atomics Méthode et système de culture de microalgues dans un réacteur à écoulement piston à expansion
US8507253B2 (en) 2002-05-13 2013-08-13 Algae Systems, LLC Photobioreactor cell culture systems, methods for preconditioning photosynthetic organisms, and cultures of photosynthetic organisms produced thereby
WO2013153402A1 (fr) * 2012-04-12 2013-10-17 Johna Ltd Procédé de culture d'algues
US10723987B2 (en) 2015-06-17 2020-07-28 Siftex Equipment Company, Inc. Pure algae growth system and method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1579556A (fr) * 1968-02-09 1969-08-29
US3763824A (en) * 1971-11-30 1973-10-09 Minnesota Mining & Mfg System for growing aquatic organisms
US4065875A (en) * 1976-09-17 1978-01-03 University Of Delaware Selective destruction of certain algae
DE4317006A1 (de) * 1993-05-17 1994-11-24 Vieh & Fleisch Gmbh Verfahren zur Kultivierung und Verwendung von Mikroalgen

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1579556A (fr) * 1968-02-09 1969-08-29
US3763824A (en) * 1971-11-30 1973-10-09 Minnesota Mining & Mfg System for growing aquatic organisms
US4065875A (en) * 1976-09-17 1978-01-03 University Of Delaware Selective destruction of certain algae
DE4317006A1 (de) * 1993-05-17 1994-11-24 Vieh & Fleisch Gmbh Verfahren zur Kultivierung und Verwendung von Mikroalgen

Cited By (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6509188B1 (en) * 1999-04-13 2003-01-21 Fraunhofer-Gesellschaft Zur Photobioreactor with improved supply of light by surface enlargement, wavelength shifter bars or light transport
ES2193845A1 (es) * 2001-07-16 2003-11-01 Seaweed Canarias S L Procedimiento para la obtencion de un multiextracto para aplicacion en agroecosistemas.
US8507253B2 (en) 2002-05-13 2013-08-13 Algae Systems, LLC Photobioreactor cell culture systems, methods for preconditioning photosynthetic organisms, and cultures of photosynthetic organisms produced thereby
EP2270132A3 (fr) * 2005-06-07 2012-07-18 HR Biopetroleum, Inc. Procédé hybride par lots et en continu pour la production d'huile et d'autres produits utiles à partir de microbes photosynthétiques
WO2007013899A2 (fr) * 2005-06-07 2007-02-01 Hr Biopetroleum, Inc. Procede hybride continu de production d'huile et autres produits utiles a partir de microbes photosynthetiques
WO2007013899A3 (fr) * 2005-06-07 2007-03-29 Hr Biopetroleum Inc Procede hybride continu de production d'huile et autres produits utiles a partir de microbes photosynthetiques
JP2008545441A (ja) * 2005-06-07 2008-12-18 エイチアール・バイオペトロリウム・インコーポレーテッド 光合成微生物からの、油および他の有用な産物の製造のための連続−バッチ・ハイブリッド法
US7770322B2 (en) 2005-06-07 2010-08-10 Hr Biopetroleum, Inc. Continuous-batch hybrid process for production of oil and other useful products from photosynthetic microbes
AU2006272954B2 (en) * 2005-06-07 2010-12-02 Hr Biopetroleum, Inc. Continuous-batch hybrid process for production of oil and other useful products from photosynthetic microbes
EP2270132A2 (fr) * 2005-06-07 2011-01-05 HR Biopetroleum, Inc. Procédé hybride par lots et en continu pour la production d'huile et d'autres produits utiles à partir de microbes photosynthétiques
US8507264B2 (en) 2006-07-10 2013-08-13 Algae Systems, LLC Photobioreactor systems and methods for treating CO2-enriched gas and producing biomass
US8110395B2 (en) 2006-07-10 2012-02-07 Algae Systems, LLC Photobioreactor systems and methods for treating CO2-enriched gas and producing biomass
US8877488B2 (en) 2006-07-10 2014-11-04 Algae Systems, LLC Photobioreactor systems and methods for treating CO2-enriched gas and producing biomass
US8859262B2 (en) 2007-04-27 2014-10-14 Algae Systems, LLC Photobioreactor systems positioned on bodies of water
US7980024B2 (en) 2007-04-27 2011-07-19 Algae Systems, Inc. Photobioreactor systems positioned on bodies of water
ES2362917A1 (es) * 2007-12-14 2011-07-15 Eni S.P.A. "procedimiento para la producción de biomasa de algas con un elevado contenido en lípidos".
CN101918534A (zh) * 2007-12-14 2010-12-15 艾尼股份公司 生产具有高脂质含量的藻生物质的方法
WO2009077087A1 (fr) * 2007-12-14 2009-06-25 Eni S.P.A. Procédé de production d'une biomasse algale ayant une teneur lipidique élevée
US9085759B2 (en) 2007-12-14 2015-07-21 Eni S.P.A. Process for the production of algal biomass with a high lipid content
ES2368282A1 (es) * 2010-03-17 2011-11-16 Universidad De Alicante Sistema de reactor abierto para el cultivo de microalgas.
WO2011113974A1 (fr) * 2010-03-17 2011-09-22 Universidad De Alicante Système de réacteur ouvert pour la culture de micro-algues
EP2584884A1 (fr) * 2010-06-23 2013-05-01 General Atomics Méthode et système de culture de microalgues dans un réacteur à écoulement piston à expansion
EP2584884A4 (fr) * 2010-06-23 2015-02-18 Gen Atomics Méthode et système de culture de microalgues dans un réacteur à écoulement piston à expansion
AU2011271149B2 (en) * 2010-06-23 2015-08-20 General Atomics Method and system for growing microalgae in an expanding plug flow reactor
WO2013153402A1 (fr) * 2012-04-12 2013-10-17 Johna Ltd Procédé de culture d'algues
US10723987B2 (en) 2015-06-17 2020-07-28 Siftex Equipment Company, Inc. Pure algae growth system and method

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Publication number Publication date
NL1014825C2 (nl) 2001-10-04
AU2001246949A1 (en) 2001-10-15
EP1272607A1 (fr) 2003-01-08

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