WO2008010737A1 - Photobioréacteur pour une culture de microorganismes photosynthétiques - Google Patents

Photobioréacteur pour une culture de microorganismes photosynthétiques Download PDF

Info

Publication number
WO2008010737A1
WO2008010737A1 PCT/PT2006/000018 PT2006000018W WO2008010737A1 WO 2008010737 A1 WO2008010737 A1 WO 2008010737A1 PT 2006000018 W PT2006000018 W PT 2006000018W WO 2008010737 A1 WO2008010737 A1 WO 2008010737A1
Authority
WO
WIPO (PCT)
Prior art keywords
culture medium
photobioreactor
cells
module
biomass
Prior art date
Application number
PCT/PT2006/000018
Other languages
English (en)
Inventor
Antonio Manuel Cordoso Marques Ferreira
Original Assignee
Tecnia Processos E Equipamentos Industriais E Ambintais
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tecnia Processos E Equipamentos Industriais E Ambintais filed Critical Tecnia Processos E Equipamentos Industriais E Ambintais
Priority to PCT/PT2006/000018 priority Critical patent/WO2008010737A1/fr
Publication of WO2008010737A1 publication Critical patent/WO2008010737A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate

Definitions

  • the invention relates to a new photobioreactor for producing biomass.
  • Photobioreactors are fermenters in which phototrophic microorganisms such as algae and cyanobacteria are cultivated, where their growth and propagation are promoted at the same time as various substances are produced by the photosynthetic cells.
  • Microalgae blue-green algae and green algae are photoautotrophic microorganisms able to use sunlight to metabolise carbon dioxide (CO 2 ) in energy-rich organic - 3 -
  • Microalgae including cyanobacteria, have the capacity to absorb light energy (photons) and store it as chemical energy through the formation of chemical bonds .
  • the basic unit of the photosynthetic apparatus is the photosystem.
  • Light energy i.e. photons
  • carotenoids and chlorophyll pigments of the photosystem antenna complex Excitation energy passes to the pigment bed towards the reaction centre.
  • Microalgae are rich in many specific and attractive compounds, some of which are very interesting as nutritional supplements, such as long-chain polyunsaturated fatty acids.
  • Other nutriceuticals derived from microalgae are vitamins and antioxidants, such as /S-carotene and astaxanthin.
  • microalgae are also used in the pharmaceutical market as they contain sterols, which can be used as building blocks for pharmaceuticals (hormones) .
  • cyanobacteria are a potential source of compounds with biomedical applications, such as antimicrobial, antiviral and anticancer compounds.
  • microalgae can also be used in environmental engineering, in the treatment of waste water, as they help to purify water. As well as reducing the concentration of nutrients in wastewaters, such as nitrogen and phosphorus, microalgae can also be used to purify an effluent containing pollutants like metals and/or radionuclides.
  • the metals in the effluent are in the form of free metal ions or they are combined in chemical bonds. The ionic form is generally the most toxic.
  • the metals can - 5 -
  • microalgae are very powerful metal ion absorbers, particularly as a result of their large negatively charged cellular surface.
  • Examples of the open systems are outdoor ponds which usually has the disadvantage of imprecise control over process parameters. These systems offer little or no control over temperature and incident light intensity, and they also have low CO 2 (carbon dioxide) utilisation efficiency due to the lack of turbulent flow and the escape of gases from the culture medium. Contamination by other microorganisms also occurs and influences the growth of the culture, as well as decreasing the quality of the product.
  • CO 2 carbon dioxide
  • Patent US3955318 refers to a method of producing an algae product and of purifying aqueous organic waste material to provide clean water.
  • Starting algae and aqueous waste are admixed in sufficient amount to provide nutrients for the algae, which are grown in symbiotic relationship with aerobic bacteria present.
  • the mixture is aerated and, if desired, carbon dioxide added, and is exposed to alternate periods of light (e.g. 1/2 second to ten seconds) and darkness (about ten times as long as the light period) to accelerate growth of the algae, harvesting the algae product to maintain the growth rate at a very high level .
  • a device for effecting agitation and intermittent light exposure includes a reaction tank having a generally vertical wall in the form of a parabolic curve, injectors debouching into the bottom of the tank opposite the parabolic-curve wall and causing circulation so that injected and other material is forced rapidly upwardly, and a baffle transversely across the upper portion of the tank.
  • the baffle supports and directs the flow of a selected - 7 -
  • a system for carrying out the method is provided.
  • Patent SE8903801 refers to an apparatus for microalgae cultivation in a fluid-based medium, which involves the medium being made to move so that objects with a solid structure and abrasive surface rest against vessel walls.
  • the vessel involved is formed to create one or more closed vertical circulation circuits for the medium with the microalgae.
  • One or more mouthpieces for gas or liquid are arranged at the bottom of each circuit, and the medium contains objects with a hard structure and abrasive surfaces, and with the same compactness as the medium and algae themselves.
  • Two parallel vertical light -permeable tubes form the vessel, the tubes being connected at the top and bottom to form a closed circuit, and one or more light sources are arranged at least between the tubes.
  • the vessel has a base and a light-permeable outer cylindrical container wall, and a similar inner wall.
  • a cylindrical screen wall is fitted concentrically between the outer and inner walls with its edges located above the base and below the surface of the medium.
  • One or more light sources are arranged at least inside the inner container wall .
  • the invention relates to a photobioreactor for cultivating algae comprising a disposable element for holding the cultivation medium and a - 8 -
  • the algae may comprise both marine and fresh water algae .
  • the photobioreactor is especially advantageous for algae which are difficult to cultivate due to contamination problems.
  • Patent JP2002262858 intends to provide a method for industrially efficiently cultivating a large amount of blue-green algae at a low cost.
  • the blue-green algae are, for example, Spirulina platensis, Spirulina maxima, Spirulina ienneri, Spirulina flavorience, Spirula laxima or Spirulina maiol .
  • the agricultural greenhouse is made of metal frames and transparent resin films cover the frames.
  • patent US4438591 the invention relates to algal cell growth, modification and harvesting, and more particularly to systems, apparatus and methods for growing, enhancing the growth of and harvesting of motile swimming microorganisms, especially unicellular algae, such as Dunaliella, which multiply by cell division. - 9 -
  • the objective of the present invention is to develop an enclosed photobioreactor, for the production of biomass, which is able to overcome the problems associated with open systems such as ponds and raceways, and which at the same time is more economic than the artificial enclosed photoreactors that already exist.
  • the present invention comprises a photobioreactor for biomass production, as defined in claim 1. It is essentially characterised in that it contains a set of transparent tubes (cells) grouped into units (modules) , each unit having a certain number of cells through which the culture medium flows vertically in an upflow direction. The total number of cells depends on the daily flow of culture medium to be processed.
  • the culture medium is provided with the necessary nutrients, carbon dioxide and - 10 -
  • the invention also relates to a process for the production of photosynthetic microorganisms, according to claim 9.
  • FIG. 1 represents one unit of the photobioreactor (one module) comprising a set of cells (tubes) aligned vertically;
  • FIG. 2 is a schematic drawing of the photobioreactor indicating the inlet, outlet and recirculation streams of the system (culture medium) .
  • the system culture medium
  • FIG. 2 is a schematic drawing of the photobioreactor indicating the inlet, outlet and recirculation streams of the system (culture medium) .
  • four units of cells are represented.
  • the photobioreactor of the present invention is proposed for the culture of photosynthetic microorganisms and it can be used for the culture of any type of photosynthetic organism having light requirements.
  • a further objective of the invention is a process - 11 -
  • the unit (3) of the photobioreactor (figure 1) essentially comprises a set of tubular cells (5) .
  • a collector (4) is connected to the bottom of all the cells (4) and another collector (6) is connected to the top of all the cells (5) .
  • the culture medium (1) enters the unit (3) by the action of the pump (2) and feeds the cells (5) from collector (4) in an upflow direction.
  • the culture medium is collected in the collector (6) .
  • the cells (5) are arranged vertically in parallel at a distance which allows light, either artificial or natural, to reach the whole surface of the cells.
  • Each of the cells is manufactured in a transparent material permitting the passage of light and the material used must be resistant to external aggressions and tension forces.
  • Figure 2 illustrates the assembly of a possible embodiment of the present invention comprising four modules
  • the culture medium (1) is pumped (2) to the first unit or module (3) of the photobioreactor through the collector (4) located at the base of the respective unit and is distributed simultaneously through all the cells (5) in an upflow direction, being collected in the collector (6) at the top of the module from where it is discharged through the tubular connection (7) to the next module (12) .
  • the culture medium is always fed to each unit from the bottom and is collected at the top. From the moment the culture medium is fed to the first module (3) until it is discharged into the last module (14) in the present case, it has to pass through all the cells (5) in all the modules
  • the culture medium is recirculated (11) by a pump (10) to the first module (3) of cells (5) and repeats the same path as before.
  • the cycles are repeated as many times as needed for the culture medium to achieve the desired concentration of products (substances) .
  • the purpose of the culture is to produce biomass of a certain microalgae, the culture medium is discharged when the desired concentration has been reached (maximum production possible) .
  • the culture medium effluent to be treated
  • the culture medium will be recirculated until the nutrient concentration has been reduced to a minimum amount .
  • the culture medium is pumped (2) through the cells (5) at a relatively high velocity, usually between 8 and 25 cm/s.
  • the culture medium should be fed (1) to the photobioreactor with a flow permitting a velocity through the cells which makes it possible to generate the turbulence necessary to prevent sedimentation of the microalgae and to ensure that there is no self-shadowing by - 13 -
  • Turbulence helps to prevent microalgae from adhering to the walls of the tubes and maximises the use of sunlight in photosynthesis, as it allows the cells to receive intermittent exposure to sunlight, which is known to improve photosynthesis efficiency.
  • the nutrients and carbon source necessary for microalgae growth such as carbon dioxide (CO 2 ) , phosphorus
  • the dilution rate of the system must be controlled in order to - 14 -
  • microalgae concentration cannot exceed a specific value, otherwise part of the culture has to be discharged (9) and fresh medium will have to be fed to the system. If the microalgae concentration reaches a very high level, problems of self- shadowing may arise leading to the creation of dark zones where the light cannot penetrate, resulting in a low efficiency of the photosynthetic process.
  • the culture unit should preferably comprise means for performing this separation, which can be achieved by various processes.
  • the biomass can be separated from the liquid phase by centrifugation or with a filter unit. For example, if the photobioreactor is applied in wastewater treatment, once the cycles in the photobioreactor have been - 15 -
  • the effluent is discharged to an ultrafiltration membrane module where the biomass is concentrated and the permeate is pure water, ready to be reused.
  • the concentrated biomass can be reintroduced into the system depending on the concentration of the culture in suspension in the photobioreactor . If the culture medium is very dense the culture must be diluted and no biomass should be reintroduced into the system, but if the culture is very diluted and needs to be concentrated, more biomass must be introduced into the system.
  • the photobioreactor of the present invention is easy to assemble and since it consists of a set of modules, each with a set of cells, if a cell is damaged there is no need to change the whole structure but only the cell in the module involved. Therefore the photobiorector can be repaired without stopping.
  • the type of structure proposed also makes it possible to introduce into or withdraw from the system nutrients or products that are lacking or are in excess.
  • the system proposed for the photobioreactor of the present invention can have automatic control, meaning that all the modules can be computer controlled, which can save time and labour.
  • Another advantage of this multi-unit system is its flexibility, since it is possible to switch from the production of one product to another without many - 16 -
  • a further objective of the invention is a process for the production of photosynthetic microorganisms which is characterised in that it comprises the following steps: a) cultivation of photosynthetic microorganisms in a photobioreactor as previously defined, in order to obtain a culture medium containing photosynthetic microorganisms; b) removal of the culture medium from the photobioreactor when the desired concentration of biomass is reached or, if necessary, for ensuring the optimum functioning of the system; c) recirculation of the remaining portion of the culture medium to the beginning of the process in order to guarantee maximum exposure to sunlight and thus increase the efficiency of the photosynthesis; d) separation of the culture medium of step b) into a solid phase containing photosynthetic microorganisms and into a liquid phase; e) feeding of the photobioreactor with the liquid phase obtained in step d) if the culture medium is too dense and needs to be diluted; - 17 -
  • step d) discharge of the liquid obtained in step d) if the culture medium in the photobioreactor is correctly diluted; g) if the photoreactor is used in the treatment of wastewater effluents, in order to reduce the concentration of nutrients such as nitrogen and phosphorus, the liquid phase obtained in step d) can be reused for different purposes outside of the system and the solid phase obtained in step c) , which corresponds to concentrated biomass, can be recirculated into the photobioreactor in order to maintain the desired concentration level in the culture medium of the photobioreactor, or it can be discharged if the concentration in the biomass culture medium is above the necessary level .
  • All the stages of the process can be controlled through means for automatically controlling the variables which affect the photobioreactor, namely computer means.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un photobioréacteur pour la culture de microorganismes photosynthétiques. La production d'une biomasse, par exemple des algues, est réalisée dans un ensemble de cellules tubulaires transparentes (5), à travers lesquelles le milieu de culture coule, lesdits tubes étant exposés à la lumière du soleil afin de favoriser la photosynthèse.
PCT/PT2006/000018 2006-07-21 2006-07-21 Photobioréacteur pour une culture de microorganismes photosynthétiques WO2008010737A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/PT2006/000018 WO2008010737A1 (fr) 2006-07-21 2006-07-21 Photobioréacteur pour une culture de microorganismes photosynthétiques

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/PT2006/000018 WO2008010737A1 (fr) 2006-07-21 2006-07-21 Photobioréacteur pour une culture de microorganismes photosynthétiques

Publications (1)

Publication Number Publication Date
WO2008010737A1 true WO2008010737A1 (fr) 2008-01-24

Family

ID=37563755

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/PT2006/000018 WO2008010737A1 (fr) 2006-07-21 2006-07-21 Photobioréacteur pour une culture de microorganismes photosynthétiques

Country Status (1)

Country Link
WO (1) WO2008010737A1 (fr)

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2319376A1 (es) * 2008-11-10 2009-05-06 Juan Luis Ripolles Romeu "fotobiorreactor".
WO2011036517A1 (fr) * 2009-09-28 2011-03-31 Harshvardhan Jaiswal Système et procédé pour faire croître un micro-organisme photosynthétique
WO2012019539A1 (fr) * 2010-08-10 2012-02-16 中国科学院青岛生物能源与过程研究所 Procédé de culture en milieu semi-sec et semi-solide pour la production industrialisée de micro-algues
CN102373150A (zh) * 2010-08-24 2012-03-14 新奥科技发展有限公司 光生物反应器和光生物培养系统
WO2012045133A1 (fr) * 2010-10-04 2012-04-12 Universidade Federal Do Rio De Janeiro - Ufrj Photobioréacteur et kit pour la culture de micro-organismes photosynthétiques, obtention de biomasse, piégeage et utilisation de gaz polluants comme source nutritionnelle pour micro-organismes photosynthétiques
ITPD20100320A1 (it) * 2010-10-22 2012-04-23 Microlabs S R L Apparato per la produzione di microorganismi fotosintetici in coltura liquida
EP2496684A2 (fr) * 2009-11-02 2012-09-12 William R. Kassebaum Système de photobioréacteur et son procédé d'utilisation
US8304209B2 (en) 2008-12-11 2012-11-06 Joule Unlimited Technologies, Inc. Solar biofactory, photobioreactors, passive thermal regulation systems and methods for producing products
US8304232B2 (en) 2009-07-28 2012-11-06 Joule Unlimited Technologies, Inc. Photobioreactors, solar energy gathering systems, and thermal control methods
US8318478B2 (en) 2009-09-09 2012-11-27 Microa As Photobioreactor
WO2013011448A1 (fr) * 2011-07-18 2013-01-24 Suez Environnement Photo-bioréacteur pour la production d'une solution algale concentrée et dispositif d'exposition de la solution, en particulier pour un tel photo-bioréacteur
CN103396938A (zh) * 2013-08-05 2013-11-20 青岛恒生源生态农业有限公司 一种光合细菌悬挂式批次管道培养装置
WO2014133793A1 (fr) 2013-02-26 2014-09-04 Heliae Development, Llc Bioréacteur tubulaire modulaire
EP2585407A4 (fr) * 2010-06-23 2015-12-30 Algevolve Llc Traitement biologique avancé de l'eau à l'aide d'algues
US10533148B2 (en) * 2014-04-25 2020-01-14 Xiamen University Membrane photobioreactor for treating nitrogen and phosphorus that are out of limits in biogas slurry and treating method thereof
CN111511892A (zh) * 2017-12-22 2020-08-07 苏伊士集团 光生物反应器
CN112961771A (zh) * 2014-12-16 2021-06-15 纳米及先进材料研发院有限公司 用于空气净化的光生物反应器系统

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995006111A1 (fr) * 1993-08-27 1995-03-02 Consiglio Nazionale Delle Ricerche Systeme mettant en ×uvre des reacteurs photobiologiques tubulaires dans la culture a l'echelle industrielle de micro-organismes photosynthetiques
DE29706379U1 (de) * 1997-04-10 1997-07-17 Preussag AG, 30625 Hannover Vorrichtung zur Durchführung von photochemischen und photokatalytischen Reaktionen und photoinduzierbaren Prozessen
GB2335199A (en) * 1998-03-11 1999-09-15 Applied Photosynthetics Limite Photobioreactor apparatus

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995006111A1 (fr) * 1993-08-27 1995-03-02 Consiglio Nazionale Delle Ricerche Systeme mettant en ×uvre des reacteurs photobiologiques tubulaires dans la culture a l'echelle industrielle de micro-organismes photosynthetiques
DE29706379U1 (de) * 1997-04-10 1997-07-17 Preussag AG, 30625 Hannover Vorrichtung zur Durchführung von photochemischen und photokatalytischen Reaktionen und photoinduzierbaren Prozessen
GB2335199A (en) * 1998-03-11 1999-09-15 Applied Photosynthetics Limite Photobioreactor apparatus

Cited By (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2319376A1 (es) * 2008-11-10 2009-05-06 Juan Luis Ripolles Romeu "fotobiorreactor".
US8304209B2 (en) 2008-12-11 2012-11-06 Joule Unlimited Technologies, Inc. Solar biofactory, photobioreactors, passive thermal regulation systems and methods for producing products
US8304232B2 (en) 2009-07-28 2012-11-06 Joule Unlimited Technologies, Inc. Photobioreactors, solar energy gathering systems, and thermal control methods
US8318478B2 (en) 2009-09-09 2012-11-27 Microa As Photobioreactor
WO2011036517A1 (fr) * 2009-09-28 2011-03-31 Harshvardhan Jaiswal Système et procédé pour faire croître un micro-organisme photosynthétique
EP2496684A4 (fr) * 2009-11-02 2015-04-15 William R Kassebaum Système de photobioréacteur et son procédé d'utilisation
EP2496684A2 (fr) * 2009-11-02 2012-09-12 William R. Kassebaum Système de photobioréacteur et son procédé d'utilisation
EP2585407A4 (fr) * 2010-06-23 2015-12-30 Algevolve Llc Traitement biologique avancé de l'eau à l'aide d'algues
WO2012019539A1 (fr) * 2010-08-10 2012-02-16 中国科学院青岛生物能源与过程研究所 Procédé de culture en milieu semi-sec et semi-solide pour la production industrialisée de micro-algues
CN102373156A (zh) * 2010-08-10 2012-03-14 中国科学院青岛生物能源与过程研究所 一种用于微藻工业化生产的半干固态培养方法
CN102373150B (zh) * 2010-08-24 2015-02-11 新奥科技发展有限公司 光生物反应器和光生物培养系统
CN102373150A (zh) * 2010-08-24 2012-03-14 新奥科技发展有限公司 光生物反应器和光生物培养系统
WO2012045133A1 (fr) * 2010-10-04 2012-04-12 Universidade Federal Do Rio De Janeiro - Ufrj Photobioréacteur et kit pour la culture de micro-organismes photosynthétiques, obtention de biomasse, piégeage et utilisation de gaz polluants comme source nutritionnelle pour micro-organismes photosynthétiques
ITPD20100320A1 (it) * 2010-10-22 2012-04-23 Microlabs S R L Apparato per la produzione di microorganismi fotosintetici in coltura liquida
WO2013011448A1 (fr) * 2011-07-18 2013-01-24 Suez Environnement Photo-bioréacteur pour la production d'une solution algale concentrée et dispositif d'exposition de la solution, en particulier pour un tel photo-bioréacteur
FR2978159A1 (fr) * 2011-07-18 2013-01-25 Suez Environnement Photo-bioreacteur pour la production d'une solution algale concentree et dispositif d'exposition de la solution, en particulier pour un tel photo-bioreacteur
WO2014133793A1 (fr) 2013-02-26 2014-09-04 Heliae Development, Llc Bioréacteur tubulaire modulaire
US10053659B2 (en) 2013-02-26 2018-08-21 Heliae Development Llc Modular tubular bioreactor
US10876087B2 (en) 2013-02-26 2020-12-29 Heliae Development Llc Modular tubular bioreactor
CN103396938A (zh) * 2013-08-05 2013-11-20 青岛恒生源生态农业有限公司 一种光合细菌悬挂式批次管道培养装置
US10533148B2 (en) * 2014-04-25 2020-01-14 Xiamen University Membrane photobioreactor for treating nitrogen and phosphorus that are out of limits in biogas slurry and treating method thereof
CN112961771A (zh) * 2014-12-16 2021-06-15 纳米及先进材料研发院有限公司 用于空气净化的光生物反应器系统
CN111511892A (zh) * 2017-12-22 2020-08-07 苏伊士集团 光生物反应器

Similar Documents

Publication Publication Date Title
WO2008010737A1 (fr) Photobioréacteur pour une culture de microorganismes photosynthétiques
Assunção et al. Enclosed “non-conventional” photobioreactors for microalga production: A review
Wang et al. Closed photobioreactors for production of microalgal biomasses
Ting et al. Progress in microalgae cultivation photobioreactors and applications in wastewater treatment: A review
Pulz et al. Photobioreactors: design and performance with respect to light energy input
SundarRajan et al. A review on cleaner production of biofuel feedstock from integrated CO2 sequestration and wastewater treatment system
Chaumont Biotechnology of algal biomass production: a review of systems for outdoor mass culture
Pires et al. Carbon dioxide capture from flue gases using microalgae: engineering aspects and biorefinery concept
Pulz Photobioreactors: production systems for phototrophic microorganisms
EP3328985B1 (fr) Bioréacteurs à diodes électroluminescentes et procédés d'utilisation
Tredici Mass production of microalgae: photobioreactors
Xu et al. Microalgal bioreactors: challenges and opportunities
KR101197244B1 (ko) 하폐수의 질소 및 인을 처리하는 장치 및 방법
Chisti MICROALGAE AS SUSTAINABLE CELL FACTORIES.
US20120214198A1 (en) Algaculture method
WO2007129327A1 (fr) Photobioréacteur destiné à cultiver et à recueillir une biomasse et son procédé
CN103789195A (zh) 一种实现原位固液分离的膜微藻光生物反应器及其培养方法
WO2010138571A1 (fr) Photobioréacteur et procédé de culture et de récolte de micro-organismes
Dębowski et al. Microalgae–cultivation methods
Pulz et al. IGV GmbH experience report, industrial production of microalgae under controlled conditions: innovative prospects
Catarina Guedes et al. Photobioreactors for cyanobacterial culturing
KR101378481B1 (ko) 혐기소화조 및 ｍｂｒ조를 이용한 미세조류 배양장치 및 방법
Rorrer Bioprocess engineering of phototrophic marine organisms
US20100136676A1 (en) Modular continuous production of micro-organisms
KR20120073432A (ko) 폐수를 이용한 미세조류 생산장치

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 06757920

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

NENP Non-entry into the national phase

Ref country code: RU

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC, DATED 22.4.09

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC, EPO FORM 1205A DATED 22.04.09

122 Ep: pct application non-entry in european phase

Ref document number: 06757920

Country of ref document: EP

Kind code of ref document: A1