WO2011036517A1 - Système et procédé pour faire croître un micro-organisme photosynthétique - Google Patents

Système et procédé pour faire croître un micro-organisme photosynthétique Download PDF

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Publication number
WO2011036517A1
WO2011036517A1 PCT/IB2009/054230 IB2009054230W WO2011036517A1 WO 2011036517 A1 WO2011036517 A1 WO 2011036517A1 IB 2009054230 W IB2009054230 W IB 2009054230W WO 2011036517 A1 WO2011036517 A1 WO 2011036517A1
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WO
WIPO (PCT)
Prior art keywords
micro
organism
water
culture
tank
Prior art date
Application number
PCT/IB2009/054230
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English (en)
Inventor
Harshvardhan Jaiswal
Original Assignee
Harshvardhan Jaiswal
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harshvardhan Jaiswal filed Critical Harshvardhan Jaiswal
Priority to PCT/IB2009/054230 priority Critical patent/WO2011036517A1/fr
Publication of WO2011036517A1 publication Critical patent/WO2011036517A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34—Chemical or biological purification of waste gases
    • B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84—Biological processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2257/00—Components to be removed
    • B01D2257/50—Carbon oxides
    • B01D2257/504—Carbon dioxide

Definitions

  • the present invention relates generally to micro-organism farming, and more particularly, to system and methods of growing photosynthetic micro-organism using a photo-bioreactor in order to achieve the fastest growth with high density per liter of water in a convenient, cost effective, and environmental friendly manner.
  • Algae are microscopic plants which use sunlight and carbon dioxide to grow and in turn release oxygen. They are the fastest growing plants on our planet. It has also been found that certain strains of algae contain a high lipid content of almost 50% which can be extracted using various methods and converted into a number of hydrocarbons. But creating the best conditions for algae growth has not been cost effective till date so that the process can compete with the traditional hydrocarbons.
  • Algae can be grown in open ponds but require constant agitation as sunlight cannot penetrate deep in a pond after an initial algae growth. This leads to very low density and thus requires a huge area. Artificial ponds can be made on land but they all face the same problem.
  • a number of closed loop systems have also been developed and are currently in use but they require a greenhouse in order to maintain the optimum conditions for algae growth. Traditional closed loop systems are highly efficient in growing algae but require a huge energy input and cost to maintain the temperature during the various climates in a year.
  • the conventional means for growing photosynthetic micro-organism fail to achieve the fastest growth with high density per liter of water in a convenient, cost effective, and environmental friendly manner. Therefore, there exists a need for effective means for growing photosynthetic micro-organism to achieve the fastest growth with high density per liter of water in a convenient, cost effective, and environmental friendly manner.
  • the general purpose of the present invention is: to provide system and methods of growing photosynthetic micro-organism by utilizing a photo-bioreactor in order to achieve the fastest growth with high density per liter of water in a convenient, cost effective, and environmental friendly manner; to include advantages of the conventional systems and methods for growing photosynthetic micro-organisms; and overcome the drawbacks inherent therein.
  • the present invention provides a system for growing photosynthetic microorganism.
  • the system comprises: a culture tank capable of retaining a micro-organism culture and the water; a cooling tank connected with the culture tank for controlling the temperature of the water of the culture tank; a holding tank capable of holding the microorganism at a required temperature and light; and at least a bioreactor capable of providing maximum exposure of sunlight to the micro-organisms and maintaining continuous flow of the micro-organism.
  • the holding tank is equipped with atleast a light source to continue micro-organism growth in the absence of adequate sunlight and temperature during the daytime and at night.
  • the present invention provides a method of growing photosynthetic micro-organism.
  • the method comprises the steps of: putting water and photosynthetic micro-organism in a culture tank; controlling the temperature of the water containing the photosynthetic micro-organism in the culture tank; adding required nutrients from a nutrient tank in the culture tank depending on the photosynthetic micro-organism to grow; pumping out the culture of micro-organism and water from the culture tank to at least a bioreactor; circulating the culture of micro-organism and water in the bioreactor; holding and storing the micro-organism culture and water in a holding tank at a required temperature and light; harvesting and drying the micro-organism if desired density of culture is achieved; and sending the culture of micro-organism back to culture tank if desired density of micro-organism is not reached.
  • the density of micro-organism culture is measured by the sensor on the basis of required time for the micro-organism to double.
  • the present invention provides a system for growing algae.
  • the system comprises: a culture tank capable of retaining algae culture and a algae growing media, the culture tank comprises a filter which is capable of separating the water holding the micro-organism and filtered water by allowing the water holding the micro-organism to automatically move to the filtered water side; a cooling tank connected with the culture tank for controlling the temperature of the water of the culture tank at a required level and keeping the water of the culture tank clean; a holding tank equipped with atleast a light source to continue micro-organism growth in the absence of adequate sunlight and temperature during the daytime and at night; and at least a bioreactor having a plurality of vertical channels capable of providing maximum exposure of sunlight to the microorganisms and maintaining continuous flow of the micro-organism, the vertical channels are configured to allow all the water and micro-organism culture from the bioreactor to completely flush out into the holding tank when air is pumped into the bioreactor from an air source.
  • FIG. 1 is a system diagram illustrating a system for growing photosynthetic microorganism, according to an exemplary embodiment of the present invention
  • FIG. 1A illustrates a bioreactor having a plurality of vertical channels in descending order for growing photosynthetic micro-organism, according to an exemplary embodiment of the present invention
  • FIG. IB illustrates a bioreactor having a plurality of vertical channels of same height for growing photosynthetic micro-organism, according to an exemplary embodiment of the present invention.
  • the terms 'a' , 'an', 'atleast' do not denote a limitation of quantity, but rather denote the presence of at least one of the referenced item, and 'a plurality' denotes the presence of more than one referenced items.
  • the present invention provides a system and method for growing algae to produce hydrocarbons and capture carbon dioxide in a cost effective, secure, and environment friendly manner; making the system secure and reliable; to include advantages of the conventional photosynthetic micro-organism growing systems and methods; and to overcome the drawbacks inherent therein.
  • the system for growing photo-synthetic micro-organism (also referred to as 'algae') to produce hydrocarbons and capture carbon dioxide is a closed loop system which does not require greenhouse to cover the whole area of the farm and is one of the most efficient process requiring minimum energy input and workforce.
  • the system according to the present invention does not use a greenhouse, the light transmittance is maximum due to unobstructed solar rays, on the contrary the minimum of 8-10% of the solar rays are absorbed by the greenhouse covers which decreases the efficient of algae growth.
  • the present invention provides a system for growing photosynthetic micro-organism.
  • the system comprises: a culture tank 20 capable of retaining a micro-organism culture and the water; a cooling tank connected with the culture tank 20 for controlling the temperature of the water of the culture tank 20; a holding tank 40 capable of holding the micro-organism at a required temperature and light; and at least a bioreactor 10 capable of providing maximum exposure of sunlight to the micro-organisms and maintaining continuous flow of the micro-organism.
  • the holding tank 40 is equipped with atleast a light source to continue micro-organism growth in the absence of adequate sunlight and temperature during the daytime and at night.
  • the present invention provides a method of growing photosynthetic micro-organism.
  • the method comprises the steps of: putting water and photosynthetic micro-organism in a culture tank 20; controlling the temperature of the water containing the photosynthetic micro-organism in the culture tank 20; adding required nutrients from a nutrient tank in the culture tank 20 depending on the photosynthetic micro-organism to grow; pumping out the culture of microorganism and water from the culture tank 20 to at least a bioreactor 10; circulating the culture of micro-organism and water in the bioreactor 10; holding and storing the microorganism culture and water in a holding tank 40 at a required temperature and light; harvesting and drying the micro-organism if desired density of culture is achieved; and sending the culture of micro-organism back to culture tank 20 if desired density of micro-organism is not reached.
  • the density of micro-organism culture is measured by the sensor on the basis of required time for the micro-organism to
  • the present invention provides a system for growing algae.
  • the system comprises: a culture tank 20 capable of retaining algae culture and a algae growing media, the culture tank 20 comprises a filter which is capable of separating the water holding the micro-organism and filtered water by allowing the water holding the micro-organism to automatically move to the filtered water side; a cooling tank connected with the culture tank 20 for controlling the temperature of the water of the culture tank 20 at a required level and keeping the water of the culture tank 20 clean; a holding tank 40 equipped with atleast a light source to continue micro-organism growth in the absence of adequate sunlight and temperature during the daytime and at night; and at least a bioreactor 10 having a plurality of vertical channels capable of providing maximum exposure of sunlight to the micro-organisms and maintaining continuous flow of the micro-organism, the vertical channels are configured to allow all the water and micro-organism culture from the bioreactor 10 to completely flush out into the holding tank 40 when air is pumped into the bioreactor 10 from an air
  • the system comprises atleast an improved photo-bioreactor 10, atleast a culture tank 20, atleast a nutrient tank, atleast a holding tank 40 (also referred to as 'night time holding tank 40'), atleast a cooling tank, an algae harvester, a solar dryer, a conveyor, valves & tubing, flue gas/C0 2 pipeline, logic controller, measurement sensors and probes, air blower/compressor, water pumps, and peristaltic pump.
  • an improved photo-bioreactor 10 atleast a culture tank 20, atleast a nutrient tank, atleast a holding tank 40 (also referred to as 'night time holding tank 40'), atleast a cooling tank, an algae harvester, a solar dryer, a conveyor, valves & tubing, flue gas/C0 2 pipeline, logic controller, measurement sensors and probes, air blower/compressor, water pumps, and peristaltic pump.
  • a photo-bioreactor 10 (also referred to as 'flat plate bioreactor' or 'bioreactor') has been separated by a plurality of vertical columns.
  • the algae culture enter the bioreactor 10 from the top on one end and then travels through the various vertical columns and exits through the bottom opening at the other end.
  • the photo-bioreactor 10 may be manufactured using any transparent material including glass and plastic of any kind as long as it has sufficient tensile strength. The material must be UV resistant or treated to prolong its use in open environment. The thickness may vary from 1 inch to 5 inches depending on the strain of algae chosen.
  • the height of the vertical compartment in the photo-bioreactor 10 may be descending as shown in the figure or may be same throughout.
  • the photo-bioreactor 10 is capable of providing the culture maximum exposure to sunlight during the day time and then to flush the culture completely out of the bioreactor 10 during night to store the culture in the holding tank 40.
  • algae may be stored in temperature controlled holding tank 40 which require very low energy input/consumption due to having less area in comparison with the very large area of the green house.
  • atleast a culture tank 20 is used in order to maintain the properties of the growing medium.
  • the culture tank 20 has two sections, one for the water holding the algae and other for filtered water.
  • the culture tank 20 is separated by a filter which allows the water to automatically move to the filtered water side.
  • the culture tank 20 As a cooling tank is used to cool the water during the summer months, the culture tank 20 needs clean water to avoid clogging.
  • the culture tank 20 is also equipped with various sensors which continuously monitor the nutrient levels and CO 2 levels in the water.
  • the culture tank 20 also equipped with nozzles on the bottom which feed the flue gasses/ CO 2 continuously to the growing medium.
  • a nutrient tank maintains a stock of premixed nutrients required by the chosen algae strain.
  • a holding tank 40 is used to flush out the algae at night when the temperatures drop and store the algae in the temperature controlled atmosphere.
  • the holding tank 40 may be equipped with a stirring mechanism to stir the water continuously so that the algae get appropriate air and don't get accumulated on the bottom.
  • the holding tank 40 may be equipped with lights to continue algae growth depending on the light cycle chosen. This reduces the cost of installing lights over a large area of land where the light dissipates in the environment rather than getting absorbed by the algae.
  • a cooling tank is used to cool the water to the desired level.
  • cooling tanks are principally based on the principle of evaporative cooling, the cooling tanks tend to work very efficiently in dry and hot weathers when the cost to cool a greenhouse is a lot more.
  • algae breeds in water only the water needs to be maintained at the required temperature and not the whole area.
  • an algae harvester is used to remove the water from the algae as much as possible and the water is re-circulated back into the system.
  • the harvester may be of various designs depending on the size and strain of algae chosen.
  • atleast a dryer is used to dry the water out from the algae.
  • the dryer may be any dryer, including but not limiting to a solar dryer.
  • the dryer may also be of various designs including an automated dryer with a conveyor bed.
  • the solar dryer may be equipped with humidity sensor and ventilation fans so that if humidity reaches a particular level, the air inside may be automatically ventilated.
  • a dispersing mechanism is chosen so that the algae are evenly distributed on the surface of a conveyor.
  • the use of this given mechanism makes the process automated so that the wet algae enter on one side of the conveyor and dry algae gets out from the other end. This keeps the process efficient and economical as very little energy input goes in the drying of the algae and very less labour hours are required to maintain the process.
  • Multiple conveyors of different sizes are used to transport algae from the harvester to the dryer and then finally for any number of operations to get the desired outcome of cultivating algae.
  • a plurality of valves and tubing may be required to control the flow of algae growth medium from the different tanks to the photo-bioreactor 10 and then to the harvester.
  • the valves and tubing will be also used to control the flow of flue gasses or CO 2 and also to evacuate the algae at night by using air to flush the bioreactor 10 at night.
  • a flue gas/CCh pipeline is used to transfer the gas from a nearby source of rich CO 2 gas to the culture tank 20 via a heat exchanger to lower the temperature of the incoming gas.
  • CO 2 is one of the most important elements to algae growth, this is a necessary requirement to the process.
  • a logic controller is used to continuously monitor all the aspects and variables for farming of algae, including temperature of water, temperature of flue gasses, nutrient levels, time of day, amount of sunlight available and various other process requirements.
  • the logic controller is capable of controlling all the valves and the complete system to automatically take the appropriate step as programmed by the user.
  • the logic controller is the most important component to make the process automated.
  • the logic controller may be a PLC or a computer or any system which may interact with the different sensors and take appropriate measures.
  • a plurality of measurement sensors and probes may be used to measure various variables to achieve an optimum algae growth. All the sensors and probes are to be connected with the logic controller.
  • an air blower is used to flush out the algae during dark hours to the holding tank 40.
  • the pressure will be controlled as per the operating specifications of the material chosen for the photo- bioreactor 10 and the tubing which carries the air.
  • a plurality of water pumps may be used to transport the algae to the photo-bioreactor 10 from the nutrient tank and to the holding tank 40 as shown in the FIG. 1.
  • a Peristaltic Pump is used to accurately dispense the nutrient amount in the culture tank 20 so that the growing media doesn't get saturated with a particular nutrient by uncontrolled dispensing of nutrients.

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  • Bioinformatics & Cheminformatics (AREA)
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Abstract

Cette invention concerne un système et un procédé pour faire croître un micro-organisme photosynthétique. Le système selon l'invention comprend : une cuve de culture capable de retenir une culture de micro-organisme et l'eau, une cuve de refroidissement reliée à la cuve de culture pour ajuster la température de l'eau de la cuve de culture ; une cuve de rétention capable de garder le micro-organisme à une température et sous une lumière requises ; et au moins un bioréacteur capable de fournir une exposition maximale à la lumière du soleil aux micro-organismes et de maintenir le flux continu du micro-organisme.
PCT/IB2009/054230 2009-09-28 2009-09-28 Système et procédé pour faire croître un micro-organisme photosynthétique WO2011036517A1 (fr)

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PCT/IB2009/054230 WO2011036517A1 (fr) 2009-09-28 2009-09-28 Système et procédé pour faire croître un micro-organisme photosynthétique

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2009/054230 WO2011036517A1 (fr) 2009-09-28 2009-09-28 Système et procédé pour faire croître un micro-organisme photosynthétique

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USD661164S1 (en) 2011-06-10 2012-06-05 Heliae Development, Llc Aquaculture vessel
US8341877B2 (en) 2011-05-31 2013-01-01 Heliae Development, Llc Operation and control of V-trough photobioreactor systems
WO2013017723A1 (fr) * 2011-08-01 2013-02-07 Algaenergy, S. A. Photobioréacteur destiné à la culture de microorganismes photoautotrophes
USD679965S1 (en) 2011-06-10 2013-04-16 Heliae Development, Llc Aquaculture vessel
USD682637S1 (en) 2011-06-10 2013-05-21 Heliae Development, Llc Aquaculture vessel
CN103897979A (zh) * 2012-12-29 2014-07-02 骆野鸣 一种内置式循环温控系统的管道式光生物反应器
EP2737046A4 (fr) * 2011-07-25 2015-08-12 Gen Atomics Système et procédé de culture d'algues utilisant la circulation d'un flux pulsé
FR3139479A1 (fr) * 2022-09-14 2024-03-15 Pierre Calleja Dispositif de captation du dioxyde de carbone comprenant au moins un support de microorganismes placé dans une atmosphère présentant un taux d’humidité élevé

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998045405A2 (fr) * 1997-04-10 1998-10-15 Preussag Ag Installation pour effectuer des reactions photochimiques et photocatalytiques et des processus photoinductifs
WO2003006629A1 (fr) * 2001-07-11 2003-01-23 Toshirou Sekine Procede et dispositif de culture de microbes de photosynthese
WO2008010737A1 (fr) * 2006-07-21 2008-01-24 Tecnia Processos E Equipamentos Industriais E Ambintais Photobioréacteur pour une culture de microorganismes photosynthétiques
WO2008051865A2 (fr) * 2006-10-20 2008-05-02 Arizona Board Of Regents For And On Behalf Of Arizona State University Systeme et procede permettant la croissance de cellules photosynthetiques
CN201102956Y (zh) * 2007-08-22 2008-08-20 上海日泰医药设备工程有限公司 一种外循环光合反应生物发酵装置

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998045405A2 (fr) * 1997-04-10 1998-10-15 Preussag Ag Installation pour effectuer des reactions photochimiques et photocatalytiques et des processus photoinductifs
WO2003006629A1 (fr) * 2001-07-11 2003-01-23 Toshirou Sekine Procede et dispositif de culture de microbes de photosynthese
WO2008010737A1 (fr) * 2006-07-21 2008-01-24 Tecnia Processos E Equipamentos Industriais E Ambintais Photobioréacteur pour une culture de microorganismes photosynthétiques
WO2008051865A2 (fr) * 2006-10-20 2008-05-02 Arizona Board Of Regents For And On Behalf Of Arizona State University Systeme et procede permettant la croissance de cellules photosynthetiques
CN201102956Y (zh) * 2007-08-22 2008-08-20 上海日泰医药设备工程有限公司 一种外循环光合反应生物发酵装置

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8341877B2 (en) 2011-05-31 2013-01-01 Heliae Development, Llc Operation and control of V-trough photobioreactor systems
US8365462B2 (en) 2011-05-31 2013-02-05 Heliae Development, Llc V-Trough photobioreactor systems
USD661164S1 (en) 2011-06-10 2012-06-05 Heliae Development, Llc Aquaculture vessel
USD679965S1 (en) 2011-06-10 2013-04-16 Heliae Development, Llc Aquaculture vessel
USD682637S1 (en) 2011-06-10 2013-05-21 Heliae Development, Llc Aquaculture vessel
EP2737046A4 (fr) * 2011-07-25 2015-08-12 Gen Atomics Système et procédé de culture d'algues utilisant la circulation d'un flux pulsé
WO2013017723A1 (fr) * 2011-08-01 2013-02-07 Algaenergy, S. A. Photobioréacteur destiné à la culture de microorganismes photoautotrophes
CN103897979A (zh) * 2012-12-29 2014-07-02 骆野鸣 一种内置式循环温控系统的管道式光生物反应器
CN103897979B (zh) * 2012-12-29 2016-06-01 昆明藻井泉香生物科技有限公司 一种内置式循环温控系统的管道式光生物反应器
FR3139479A1 (fr) * 2022-09-14 2024-03-15 Pierre Calleja Dispositif de captation du dioxyde de carbone comprenant au moins un support de microorganismes placé dans une atmosphère présentant un taux d’humidité élevé

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