WO2001042494A3 - Testsystem zum nachweis von analyten sowie ein verfahren zur herstellung und verwendung - Google Patents

Testsystem zum nachweis von analyten sowie ein verfahren zur herstellung und verwendung Download PDF

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Publication number
WO2001042494A3
WO2001042494A3 PCT/EP2000/010336 EP0010336W WO0142494A3 WO 2001042494 A3 WO2001042494 A3 WO 2001042494A3 EP 0010336 W EP0010336 W EP 0010336W WO 0142494 A3 WO0142494 A3 WO 0142494A3
Authority
WO
WIPO (PCT)
Prior art keywords
production
test system
detecting analytes
analytes
conjugates
Prior art date
Application number
PCT/EP2000/010336
Other languages
English (en)
French (fr)
Other versions
WO2001042494A2 (de
Inventor
Petra Burgstaller
Dirk Konz
Original Assignee
Aventis Res & Tech Gmbh & Co
Petra Burgstaller
Dirk Konz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Res & Tech Gmbh & Co, Petra Burgstaller, Dirk Konz filed Critical Aventis Res & Tech Gmbh & Co
Priority to AU13859/01A priority Critical patent/AU1385901A/en
Priority to EP00975889A priority patent/EP1240356A2/de
Priority to JP2001544366A priority patent/JP2003528298A/ja
Priority to CA002393703A priority patent/CA2393703A1/en
Publication of WO2001042494A2 publication Critical patent/WO2001042494A2/de
Publication of WO2001042494A3 publication Critical patent/WO2001042494A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies

Abstract

Die Erfindung betrifft einen in-vitro Assay zum empfindlichen und spezifischen direkten Nachweis von Analyten. Hierbei werden als Detektor-Amplifikator-System Polypeptid-Nukleinsäure-Konjugate vorteilhaft verwendet und deren Signale mittels PCR verstärkt.
PCT/EP2000/010336 1999-12-10 2000-10-20 Testsystem zum nachweis von analyten sowie ein verfahren zur herstellung und verwendung WO2001042494A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU13859/01A AU1385901A (en) 1999-12-10 2000-10-20 Test system for detecting analytes, a method for the production thereof and its use
EP00975889A EP1240356A2 (de) 1999-12-10 2000-10-20 Testsystem zum nachweis von analyten sowie ein verfahren zur herstellung und verwendung
JP2001544366A JP2003528298A (ja) 1999-12-10 2000-10-20 分析物を検出するためのアッセイシステムおよびその調整方法およびその使用
CA002393703A CA2393703A1 (en) 1999-12-10 2000-10-20 Assay system for detecting analytes and a method for the preparation thereof and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19959857.6 1999-12-10
DE19959857A DE19959857C1 (de) 1999-12-10 1999-12-10 Testsystem zum Nachweis von Analyten sowie ein Verfahren zur Herstellung und Verwendung

Publications (2)

Publication Number Publication Date
WO2001042494A2 WO2001042494A2 (de) 2001-06-14
WO2001042494A3 true WO2001042494A3 (de) 2002-05-02

Family

ID=7932340

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/010336 WO2001042494A2 (de) 1999-12-10 2000-10-20 Testsystem zum nachweis von analyten sowie ein verfahren zur herstellung und verwendung

Country Status (6)

Country Link
EP (1) EP1240356A2 (de)
JP (1) JP2003528298A (de)
AU (1) AU1385901A (de)
CA (1) CA2393703A1 (de)
DE (1) DE19959857C1 (de)
WO (1) WO2001042494A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005081908A2 (en) 2004-02-20 2005-09-09 The Trustees Of The University Of Pennsyvania Reagents, kits and methods for immunodetection of epitopes on molecules
GB201218909D0 (en) * 2012-10-22 2012-12-05 Univ Singapore Assay for the parallel detection of biological material based on PCR

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5665539A (en) * 1991-07-12 1997-09-09 The Regents Of The University Of California Immuno-polymerase chain reaction system for antigen detection
WO1998031700A1 (en) * 1997-01-21 1998-07-23 The General Hospital Corporation Selection of proteins using rna-protein fusions
WO1999051773A1 (en) * 1998-04-03 1999-10-14 Phylos, Inc. Addressable protein arrays
WO2001016352A1 (en) * 1999-08-27 2001-03-08 Phylos, Inc. Methods for encoding and sorting in vitro translated proteins

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6083689A (en) * 1990-10-16 2000-07-04 Bayer Corporation Sensitive immunoassays utilizing antibody conjugates with replicable DNA templates
US5922553A (en) * 1996-11-21 1999-07-13 Trustees Of The University Of Pennsylvania Method of detecting protein by immuno RNA

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5665539A (en) * 1991-07-12 1997-09-09 The Regents Of The University Of California Immuno-polymerase chain reaction system for antigen detection
WO1998031700A1 (en) * 1997-01-21 1998-07-23 The General Hospital Corporation Selection of proteins using rna-protein fusions
WO1999051773A1 (en) * 1998-04-03 1999-10-14 Phylos, Inc. Addressable protein arrays
WO2001016352A1 (en) * 1999-08-27 2001-03-08 Phylos, Inc. Methods for encoding and sorting in vitro translated proteins

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
NIEMEYER C M ET AL: "OLIGONUCLEOTIDE-DIRECTED SELF-ASSEMBLY OF PROTEINS: SEMISYNTHETIC DNA-STREPTAVIDIN HYBRID MOLECULES AS CONNECTORS FOR THE GENERATION OF MACROSCOPIC ARRAYS AND THE CONSTRUCTION OF SUPRAMOLECULAR BIOCONJUGATES", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 22, no. 25, 1994, pages 5530 - 5539, XP000645135, ISSN: 0305-1048 *
ROBERTS R W ET AL: "RNA-PEPTIDE FUSIONS FOR THE IN VITRO SELECTION OF PEPTIDES AND PROTEINS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 94, no. 23, November 1997 (1997-11-01), pages 12297 - 12302, XP002913434, ISSN: 0027-8424 *
TAKESHI SANO ET AL: "IMMUNO-PCR: VERY SENSITIVE ANTIGEN DETECTION BY MEANS OF SPECIFIC ANTIBODY-DNA CONJUGATES", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 258, no. 5079, 2 October 1992 (1992-10-02), pages 120 - 122, XP000384402, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
AU1385901A (en) 2001-06-18
DE19959857C1 (de) 2001-06-28
JP2003528298A (ja) 2003-09-24
WO2001042494A2 (de) 2001-06-14
CA2393703A1 (en) 2001-06-14
EP1240356A2 (de) 2002-09-18

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