WO2001042432A3 - Procedes de production de nouvelles enzymes - Google Patents

Procedes de production de nouvelles enzymes Download PDF

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Publication number
WO2001042432A3
WO2001042432A3 PCT/GB2000/004661 GB0004661W WO0142432A3 WO 2001042432 A3 WO2001042432 A3 WO 2001042432A3 GB 0004661 W GB0004661 W GB 0004661W WO 0142432 A3 WO0142432 A3 WO 0142432A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzymes
subdomain
swaps
beta
substrate
Prior art date
Application number
PCT/GB2000/004661
Other languages
English (en)
Other versions
WO2001042432A2 (fr
Inventor
Alan Fersht
Myriam Altamirano
Original Assignee
Medical Res Council
Alan Fersht
Myriam Altamirano
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Res Council, Alan Fersht, Myriam Altamirano filed Critical Medical Res Council
Priority to AU17205/01A priority Critical patent/AU1720501A/en
Publication of WO2001042432A2 publication Critical patent/WO2001042432A2/fr
Publication of WO2001042432A3 publication Critical patent/WO2001042432A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Cette invention se rapporte à des procédés servant à obtenir des enzymes qui fixent des substrats cibles et qui catalysent des réactions souhaitées. Des protéines à barreau α/β sont classées en deux classes sur la base de la structure des couvercle catalytiques. Les couvercles peuvent être greffés sur des échafaudages, avec des modifications mineures additionnelles au niveau des résidus conservés et non conservés, pour former des enzymes de produits candidats permettant de trier les propriétés souhaitées. La conception d'une nouvelle enzyme qui fixe un substrat cible et qui catalyse une réaction choisie est facilitée par la sélection d'un échafaudage qui fixe le substrat et d'un couvercle catalytique de la bonne classe pour la réaction souhaitée. Une mutagenèse ciblée ou focalisée peut être utilisée pour affiner la fixation du substrat et la catalyse.
PCT/GB2000/004661 1999-12-08 2000-12-06 Procedes de production de nouvelles enzymes WO2001042432A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU17205/01A AU1720501A (en) 1999-12-08 2000-12-06 Methods of producing novel enzymes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9929061.1 1999-12-08
GBGB9929061.1A GB9929061D0 (en) 1999-12-08 1999-12-08 Methods of producing novel enzymes

Publications (2)

Publication Number Publication Date
WO2001042432A2 WO2001042432A2 (fr) 2001-06-14
WO2001042432A3 true WO2001042432A3 (fr) 2002-05-10

Family

ID=10865962

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/004661 WO2001042432A2 (fr) 1999-12-08 2000-12-06 Procedes de production de nouvelles enzymes

Country Status (3)

Country Link
AU (1) AU1720501A (fr)
GB (2) GB9929061D0 (fr)
WO (1) WO2001042432A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU3040101A (en) * 2000-02-03 2001-08-14 Domantis Limited Combinatorial protein domains
AU2001277614A1 (en) * 2000-08-07 2002-02-18 Domantis Limited Novel proteins
US20020183937A1 (en) * 2001-02-09 2002-12-05 Stephen Mayo Method for the generation of proteins with new enzymatic function
ES2373658T3 (es) * 2003-06-18 2012-02-07 Bayer Pharma Aktiengesellschaft Nuevas entidades biológicas y el uso de las mismas.
WO2007047995A2 (fr) 2005-10-21 2007-04-26 Catalyst Biosciences, Inc. Protéases modifiées qui inhibent l'activation du complément
US9493747B2 (en) 2008-01-03 2016-11-15 The General Hospital Corporation Engineered transglutaminase barrel proteins

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994005772A1 (fr) * 1992-09-08 1994-03-17 Rutgers, The State University Of New Jersey Enzymes ameliorees pour la production d'acide 2-ceto-l-gulonique

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994005772A1 (fr) * 1992-09-08 1994-03-17 Rutgers, The State University Of New Jersey Enzymes ameliorees pour la production d'acide 2-ceto-l-gulonique

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
ALTAMIRANO MYRIAM M ET AL: "Directed evolution of new catalytic activity using the alpha/beta-barrel scaffold.", NATURE (LONDON), vol. 403, no. 6770, 10 February 2000 (2000-02-10), pages 617 - 622, XP002173865, ISSN: 0028-0836 *
CHEN Z ET AL: "COMPLEMENTING AMINO ACID SUBSTITUTIONS WITHIN LOOP 6 OF THE ALPHA-BETA-BARREL ACTIVE SITE INFLUENCE THE CARBON DIOXIDE OXYGEN SPECIFICITY OF CHLOROPLAST RIBULOSE-1 5-BISPHOSPHATE CARBOXYLASE-OXYGENASE", BIOCHEMISTRY, vol. 30, no. 36, 1991, pages 8846 - 8850, XP002173864, ISSN: 0006-2960 *
DARIMONT BEATRICE ET AL: "Mutational analysis of the active site of indoleglycerol phosphate synthase from Escherichia coli.", PROTEIN SCIENCE, vol. 7, no. 5, May 1998 (1998-05-01), pages 1221 - 1232, XP000993562, ISSN: 0961-8368 *
FERSHT ALAN R ET AL: "Designing new enzymes.", BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 28, no. 3, 2000, 671st Meeting of the Biochemical Society.;England, UK; April 11-13, 2000, pages A53, XP000993578, ISSN: 0300-5127 *
HOPFNER K P ET AL: "New enzyme lineages by subdomain shuffling", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,NATIONAL ACADEMY OF SCIENCE. WASHINGTON,US, vol. 95, no. 17, August 1998 (1998-08-01), pages 9813 - 9818, XP002103693, ISSN: 0027-8424 *
O'BRIEN PATRICK J ET AL: "Catalytic promiscuity and the evolution of new enzymatic activities.", CHEMISTRY & BIOLOGY (LONDON), vol. 6, no. 4, April 1999 (1999-04-01), pages R91 - R105, XP000993553, ISSN: 1074-5521 *
PUJADAS GERARD ET AL: "TIM barrel fold: Structural, functional and evolutionary characteristics in natural and designed molecules.", BIOLOGIA (BRATISLAVA), vol. 54, no. 3, June 1999 (1999-06-01), pages 231 - 254, XP000993511, ISSN: 0006-3088 *
STEHLIN CATHERINE ET AL: "Deletion mutagenesis as a test of evolutionary relatedness of indoleglycerol phosphate synthase with other TIM barrel enzymes.", FEBS LETTERS, vol. 403, no. 3, 1997, pages 268 - 272, XP002173863, ISSN: 0014-5793 *
WILMANNS M ET AL: "THREE-DIMENSIONAL STRUCTURE OF THE BIFUNCTIONAL ENZYME PHOSPHORIBOSYLANTHRANILATE ISOMERASE INDOLEGLYCEROLPHOSPHATE SYNTHASE FROM ESCHERICHIA-COLI REFINED AT 2.0 A RESOLUTION", JOURNAL OF MOLECULAR BIOLOGY, vol. 223, no. 2, 1992, pages 477 - 508, XP000993512, ISSN: 0022-2836 *

Also Published As

Publication number Publication date
GB0029781D0 (en) 2001-01-17
WO2001042432A2 (fr) 2001-06-14
GB2358633A (en) 2001-08-01
AU1720501A (en) 2001-06-18
GB9929061D0 (en) 2000-02-02

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