WO2001038637A1 - Produits en papier renfermant un agent de reticulation biologique - Google Patents

Produits en papier renfermant un agent de reticulation biologique Download PDF

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Publication number
WO2001038637A1
WO2001038637A1 PCT/IL2000/000775 IL0000775W WO0138637A1 WO 2001038637 A1 WO2001038637 A1 WO 2001038637A1 IL 0000775 W IL0000775 W IL 0000775W WO 0138637 A1 WO0138637 A1 WO 0138637A1
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WO
WIPO (PCT)
Prior art keywords
cbd
cellulose
polymer containing
containing product
carbohydrate polymer
Prior art date
Application number
PCT/IL2000/000775
Other languages
English (en)
Inventor
Abraham Tenenbaum
Original Assignee
American Israeli Paper Mills Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Israeli Paper Mills Ltd. filed Critical American Israeli Paper Mills Ltd.
Priority to AU15467/01A priority Critical patent/AU1546701A/en
Publication of WO2001038637A1 publication Critical patent/WO2001038637A1/fr

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Classifications

    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
    • D21H17/20—Macromolecular organic compounds
    • D21H17/21—Macromolecular organic compounds of natural origin; Derivatives thereof
    • D21H17/22—Proteins
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B15/00—Preparation of other cellulose derivatives or modified cellulose, e.g. complexes
    • C08B15/10—Crosslinking of cellulose
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M15/00—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment
    • D06M15/01—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment with natural macromolecular compounds or derivatives thereof
    • D06M15/15—Proteins or derivatives thereof
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
    • D21H17/005—Microorganisms or enzymes
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H21/00—Non-fibrous material added to the pulp, characterised by its function, form or properties; Paper-impregnating or coating material, characterised by its function, form or properties
    • D21H21/14—Non-fibrous material added to the pulp, characterised by its function, form or properties; Paper-impregnating or coating material, characterised by its function, form or properties characterised by function or properties in or on the paper
    • D21H21/18—Reinforcing agents

Definitions

  • This invention is related to the field of industrial paper production. More
  • the invention relates to improved paper products, such as
  • Paper products consist largely of cellulose fibers. The fibers are connected
  • Paper products such as paper for
  • hydrophobic material Usually, wax or derivatives thereof are used for this
  • Chemicals used in this process comprise e.g., polyamides and
  • cellulose binding agent is derived from Acetobacter and consists of
  • microfibrillated cellulose obtained by treatment by alkali, ph >13, at 60°C
  • EP 212,289 describes a cellulose fiber material with increased absorbent
  • This material involves beating plant material in order to break cell walls
  • cross-linking process involves treatment of cellulose fibers with
  • cross-linking agents such as glyoxal, formaldehyde, or the sodium
  • resulting material is used as tampon or as surgical dressing.
  • EP 243,151 relates to the cross-linking of cellulose
  • the so-treated cellulose materials are useful as wound covers,
  • the C. fimi endoglucanase-derived cellulose binding region exhibits a
  • the protein is dissociated substantially
  • Thermo monospora endoglucanase relates to cloning of Thermo monospora endoglucanase. This enzyme binds
  • CDB cellulose binding domain
  • CBD binds cellulose with high affinity. Once bound, CBD cannot be dissociated from the cellulose by water, but only by high concentrations
  • the cellulose matrix could serve as
  • CBD binding region of CBD
  • cellulose binding domains may be used as cross-linking agents to
  • the recycling is based on re-pulping of the waste paper under
  • the invention is directed to a carbohydrate polymer containing product
  • the carbohydrate substrate is a cellulose-based cross-linking agent (BCA).
  • BCA cross-linking agent
  • the carbohydrate substrate of the invention is N-(2-a carbohydrate substrate.
  • the carbohydrate preferably, the carbohydrate
  • substrate is also characterized by improved dry tensile strength.
  • the BCA comprises one or more cellulose binding domains.
  • binding domain is preferably derived from a bacterial source, more
  • the CBD binds crystalline
  • the CBD binds Cellulose I. Further preferably,
  • the CBD is preferably by a kd of between about 0.8 to about 1.5 ⁇ M. Also preferably, the CBD
  • the BCA preferably comprises two CBDs.
  • the CBDs preferably comprises two CBDs.
  • BCA comprises a chemical cross-linking moiety reactable with cellulose.
  • the chemical cross-linking moiety is preferably an aldehyde group, a
  • glyoxal group or a sulfone group.
  • the BCA comprises two CBDs
  • the linker may be derived from a bifunctional
  • cross-linking agent such as disuccinimidyl tartrate (DSS), dimethyl
  • DSG disuccinimidyl suberate
  • DSS disuccinimidyl suberate
  • CBD regions is a peptide linker.
  • CBD regions are recombinantly produced as a single chain protein.
  • the binding of the CBD to the cellulose is not disrupted by water.
  • the binding of the CBD to cellulose is not disrupted by high
  • the CBD is preferably derived from a cbdA gene of a bacterium. More
  • the CBD is derived from the cbdA gene of C. Cellulovorans. Most preferably, the CBD comprises 162 amino acids of the CBD as
  • the cellulose substrate is preferably, but not limitatively, crystalline
  • the cellulose is in the form of fibers. Most
  • the cellulose is a paper, paperboard, or the like material.
  • cellulose may also be a cotton, rayon, or similar type material.
  • the invention also relates to a process for the manufacture of an improved
  • the process of the invention comprises the steps of
  • a cellulose material such as paper or paperboard consists of a
  • Fiber source The fibers may be derived from several sources as follows:
  • the fibers may be used in dried form (for shipment). These fiber bales
  • Fibers from a recycling process may be used. Different waste paper
  • the waste paper is immersed in water and separated to
  • the water is heated and/or chemicals are added
  • containing a CBD may be added here as this provides maximum
  • Fibers from the different sources are "refined" prior to the paper sheet
  • the CBD -containing agent is preferably
  • CBD-fiber bonds that were developed prior to this stage.
  • the fibers are diluted with water to a low consistency (0.2%- 1.5%
  • additives are added to improve paper quality or to improve paper machine
  • CBD -containing agents may be
  • the sheet surface may be coated (size-press).
  • starch is used, but other materials may also be utilized, such as
  • CBD may
  • CBD be added in this stage.
  • the substrate is
  • cellulose more preferably cellulose derived from plants, most preferably
  • the substrate is
  • the invention is directed to the
  • the invention is directed to the
  • Fig. 1A shows a scheme of CBD-crosslinked cellulose fibers, wherein the
  • linear strands represent cellulose fibers, to which the CBD (square forms)
  • CBD molecules are attached. Two CBD molecules are bound to each other via a linker
  • Fig. IB schematically shows the attachment of 'a functional group or
  • Fig. 2 shows a scheme of the expression vector used for CBD, wherein Ori
  • bla denotes the antibiotic
  • the empty box 3' to the cbdA gene denotes an optional
  • T7 denotes the T7 polymerase promoter
  • Fig. 3 shows a scheme of the expression vector for a bifunctional CBD
  • polymers according to the invention include wood-derived materials
  • wood pulp such as wood pulp, plant-derived materials such as paper, paperboard,
  • a structured cellulose fiber material such as
  • microcrystalline cellulose microcrystalline cellulose, microfiber-containing cellulose, pulp, and the
  • BCA - biological cross-linking agent
  • the BCA may be a cross-linking agent, being
  • CBD it comprises to another substance, such as a
  • the BCA may comprise the substance that it is
  • cellulose product desired to add to the cellulose product, and may bind said substance to
  • a specimen such as a paper specimen that is compressed between two
  • the sample e.g., then paper sample, is soaked in water before
  • the invention is directed to a carbohydrate polymer containing product
  • the carbohydrate substrate is a cellulose-based cross-linking agent (BCA).
  • BCA cross-linking agent
  • the carbohydrate substrate of the invention is N-(2-a carbohydrate substrate.
  • the carbohydrate substrate is also characterized by improved
  • the BCA comprises one or more cellulose binding domains from any
  • the cellulose binding domain (CBD) can be, for instance,
  • C. cellulo ⁇ orans derived from a bacterial source, such as C. cellulo ⁇ orans.
  • the CBD binds crystalline
  • the CBD binds Cellulose I. Further preferably,
  • the binding affinity of the CBD to the cellulose substrate is characterized by a kd of between about 0.8 to about 1.5 ⁇ M.
  • the BCA preferably comprises two CBDs.
  • the BCA preferably comprises two CBDs.
  • cross-linking agent is used, this is due to the fact that the cross-linking
  • the present invention overcomes all of these problems associated with
  • cross-linking material of the present invention is a
  • CBD a protein
  • the biological cross-linking agent (BCA) comprises two CBD
  • cross-linking CBD protein by cross-linking CBD protein.
  • Cross-linking proteins is well known in the
  • Proteins may be cross-linked by their functional
  • Chemical groups that react with SH groups include e.g., dithio
  • pyridyldithio groups including pyridyldithio groups, haloacetamido groups, including
  • iodoacetamido groups maleimido groups, including alkylmaleimido
  • Amino groups may be coupled using optionally sulfonated N-hydroxysuccinimide ester
  • imidoester groups including methyl pimelimidate and methyl
  • a protein may be used for cross-linking, e.g. using an amino group such as
  • the preferred CBD is the CBD derived from C. cellulovorans, disclosed in
  • residues may be advantageously used in cross-linking the CBD molecule.
  • the CBD may be cross-linked to a second CBD. In that case, the
  • cross-linker should be bifunctional.
  • the cross-linker may be any organic compound
  • cross-linker may
  • cleavable cross-linker may be any suitable cleavable cross-linker.
  • DSS disuccinimidyl suberate
  • DSG disuccinimidyl glutarate
  • DMS dimethyl suberimidate
  • cross-linkers include m-maleimideobenzoyl-N-hydroxysuccinimide ester
  • MBS N-gamma-maleimidobutyryloxy-succinimide ester
  • DST disuccinimidyl tartrate
  • a cross-linker is capable of reacting unspecifically with proteins
  • photoactivation for instance by photoactivation.
  • photoreactive groups are examples of photoreactive groups
  • photoreactive cross-linkers examples include
  • the CBD domain may also be cross-linked directly to cellulose. In this
  • a carbohydrate-reactive cross-linker is used.
  • Carbohydrate reactive groups include e.g., the aldehyde group, the glyoxal
  • MPBH 4-(4-N-maleimidophenyl)-butyric acid hydrazide
  • CBD domain of the invention does not comprise cysteine
  • M2C2H or MPBH e.g., 4-(4- (succimmido-N-oxo)-phenyl)-butyric acid
  • the protein may then be coupled via the second functionality
  • amino groups such as an activated N-hydroxy succinimide ester group.
  • cross-linkers are commercially available, e.g., from the
  • the cross-linker should advantageously comprise a spacer.
  • the spacer is
  • cross-linkers of US 5,002,883 may be used to cross-link the
  • CBD domain of the present invention to a second CBD domain.
  • US 5,399,501 describes the conjugation of immunologically active proteins
  • thiols e.g. maleimide
  • cross-linker that binds to
  • thiols e.g. maleimide
  • This set of cross-linkers may also be considered cross-linkers.
  • CBD protein comprises amino groups.
  • the CBD protein is cross-linked
  • cross-linker capable of reacting with carbohydrates on the one hand
  • ABS hydrazide
  • this "activated" CBD may then be reacted with cellulose fibers
  • CBD-cellulose bond may then be allowed to form, depending upon the
  • reaction conditions such as concentration of salt, presence of caustic solutions, reaction time, and the like, to form the desired product
  • the cross-linking of CBD proteins may be carried out using a peptide linker.
  • the peptide linker is a peptide of suitable amino acid sequence which is
  • the linker peptide may be connected to the CBD protein by a
  • cross-linker as described above for linking proteins.
  • Lysine or Arginine is chosen when it is desired to
  • Cysteine residues are chosen when it is
  • carboxylic acid may be chosen when it is desired to use carboxylic acid groups for
  • the linker is preferably between 10 and 50 amino acids in length.
  • the linker comprises small, uncharged amino acids, such as
  • the linker contains preferably
  • residues is preferably about 3:1 to 4:1.
  • Glycine/Serine linker is GGGGSGGGGSGGGGSGG.
  • two CBD peptides may be linked via a peptide, by using
  • the coding sequence for CBD is fused with
  • the entire cassette may be
  • the CBD protein may be expressed as a fusion protein with
  • Such domains comprise e.g.,
  • yeast two-hybrid screening emthod which allow the
  • transcription factor proteins such as the jun and fos
  • the avidin molecule is a peptide originally isolated
  • This peptide may be expressed as a fusion
  • the fusion protein with the CBD domain.
  • the fusion protein or alternatively,
  • unfused CBD protein may then be cross-linked to biotin, which is a small
  • the present invention is directed to a substrate cross-linked with a
  • the substrate is a cellulose substrate.
  • the cellulose substrate is preferably crystalline cellulose, more preferably
  • the substrate is characterized by
  • Wet strength may be measured under various conditions, which are
  • the tensile modulus of the material is determined according to
  • paper materials are used as paperboard containers, e.g., for
  • temperatures including cold conditions, such as 5-10 degrees Celsius.
  • the BCA comprises a cellulose binding domain.
  • CBD is preferably derived from a bacterial source, more preferably from C. cellulo ⁇ orans. Most preferably, the CBD is the 162
  • the CBD binds crystalline
  • the CBD binds Cellulose I. Further preferably,
  • the CBD does
  • CBD is preferably produced by molecular biology methods. Such methods, e.g.,
  • CBD gene is cloned into an expression vector, such as the pET
  • the promoter used is preferably a bacterial phage promoter, such as
  • T3 or T7 promoter require the presence in the
  • CBD protein or CBD -linker- CBD faces no
  • mRNA can be produced, using these conditions.
  • CBD is purified from bacterial extracts by methods as known in the art.
  • the protein readily is solubilized by addition of 6M
  • CBD protein may be used directly as crude bacterial extract, which may be
  • solubilization agent at least to a point that allows the CBD to regain its
  • the binding of CBD to cellulose is measured preferably by methods known
  • CBD -cellulose binding constant and cellulose binding capacity of CBD are examples of CBD.
  • purified CBD is produced as described in the above US
  • the protein (about 1-50 ⁇ g) is added to a solution containing
  • cellulose (1 mg) in phosphate buffer at neutral pH, e.g., PBS or 50 mM
  • the assay tubes are
  • the pellet may then be determined by a protein quantification method as known in the art. A residual amount of BSA will be detected in the pellet.
  • the binding of the CBD to the cellulose is not disrupted by water.
  • the binding of the CBD to cellulose is not disrupted by high
  • the CBD is preferably derived from a cbdA gene of a bacterium. More
  • the CBD is derived from the cbdA gene of C. Cellulovorans.
  • the CBD comprises 162 amino acids of the CBD as
  • the cellulose substrate is preferably crystalline cellulose. More preferably,
  • the cellulose is in the form of fibers. Most preferably, the cellulose is a
  • the cellulose may also be a cotton
  • the invention also relates to a process for the manufacture of an improved
  • the process of the invention comprises the steps of contacting a cellulose
  • a cellulose substrate such as
  • CBD is a cellulose substrate incubated with CBD
  • activated CBD is coupled to the cellulose, is carried out.
  • the incubation buffer is preferably a low-salt buffer adjusted to neutral
  • protein may be derived from the bacterial extract, if crude bacterial
  • CBD extract is used, or if partially purified CBD is used. Alternatively, the
  • carrier protein may be added to the incubation solution.
  • concentrations for the carrier protein range from about 0.2 to about 2.5
  • the carrier protein is preferably
  • the cellulose substrate may comprise long fibers of cellulose, or short
  • fibers such as microfibrils, obtained by beating the plant source material
  • Microfibrils may also be added to a cellulose
  • microfibrils to a cellulose
  • the substrate is
  • cellulose more preferably cellulose derived from plants, most preferably
  • the substrate is
  • the invention is directed to the
  • the invention is directed to the
  • the invention thus provides a cellulose substrate wherein cellulose
  • strands including fibrils are cross-linked by CBD domains.
  • the invention provides a towel comprising cellulose substrate
  • the invention further provides a paper, particularly a paper label for a
  • the invention also provides a paper, paperboard, or corrugated paperboard
  • the corrugated paperboard preferably consists of outer
  • the inner (corrugated) layer may be cross-linked by a
  • paperboard, or corrugated paperboard is used preferably in the
  • packages are for transport of goods by sea.
  • the packages are for use in transporting
  • the packages are for use as storage
  • freezers preferably provide a temperature of at least from —20 to —40
  • the packages are
  • the packages are for the transportation and storage of
  • the packages are for use in the transportation and storage of
  • products that require chilling such as medicaments, biochemical reagents,
  • the invention provides cellulose substrate
  • the invention provides cellulose substrate
  • CBD protein is produced and purified, preferably as
  • spacer is then attached to the purified CBD, via the succinimide group.
  • sulfo-SMPB is used.
  • Cross-linkers containing other spacers may also be used.
  • SMPB short spacer arm (14.5 Angstrom)
  • CBD protein in PBS phosphate-buffered saline
  • the CBD solution is then adjusted to its original volume, and
  • DSS disuccinimidyl suberate
  • the mixture is incubated for 2 hrs at RT and tilted gently
  • CBD protein binds to the amino groups of the CBD protein, thereby cross-linking CBD
  • the buffer using ultrafiltration or dialysis.
  • the buffer using ultrafiltration or dialysis.
  • the buffer using ultrafiltration or dialysis.
  • reaction is incubated with gentle tilting either overnight at 4°C or for l-2h
  • cross-linker used contained maleimide or other labile groups
  • Maleimide groups may be quenched by
  • reactive groups may be quenched by incubation with amino acids such as
  • the quenching reagent is added at a concentration of
  • the cellulose substrate is then washed, optionally sonicated or treated
  • 5,837,814 is cloned into an expression vector, preferably the pET vector.
  • preferred amino acid linker is the sequence GGGGSGGGGSGGGGSGG.
  • the CBD coding sequence is inserted a
  • CBD coding sequence derived from a
  • fusion protein is then expressed in a bacterial host, preferably a high-yield
  • protease-deficient bacterial host such as the E. coli strain BL-21.
  • CBD fusion protein The production and purification of CBD fusion protein is carried out
  • the CBD domain Briefly, the CBD fusion protein is then introduced into
  • plasmid are identified by selection of colonies under selective pressure
  • Luria-Bertani or other suitable medium preferably NZCYM medium
  • CBD fusion protein is induced by the addition of IPTG to ImM final
  • the cells are then lysed by repeated sonication on ice.
  • the precipitated protein is
  • the CBD fusion protein and remove traces of guanidinium salt.
  • the purified CBD fusion protein is then tested for its ability to bind to
  • Bacterial strains producing high amounts of highly active CBD fusion protein are selected for large-scale
  • the bacterial are grown in a rich medium, such as Luria-Bertani, 2YT, or
  • bacterial culture is allowed to grow to a density that is most suitable for
  • This density will generally be between 0.2 and 1.0
  • the media used (bacteria grown in 2YT or TB may be grown to higher
  • IPTG IPTG
  • the bacterial cell wall may now be broken by mechanical force, e.g., using
  • the extract may be used directly for incubation with the
  • extract may be treated with 6 M guanidinium salt, in order to dissolve
  • This treated extract may then
  • the treated and diluted extract may be used
  • the fusion protein may be
  • This Example describes the preparation of a paper sheet according to the
  • Virgin fibers derived from wood pulp may be derived from various sources.
  • Virgin fibers derived from wood pulp may be derived from various sources.
  • Dried fiber bales are suspended in water by immersion and
  • Fibers from a recycling process may also be used as a recycling process.
  • Fibers that were prepared from recycled old corrugated boxes are
  • Fibers derived from recycled labels are used in production of tissue
  • the waste paper is immersed in water and
  • the water is optionally heated
  • suspended fibers are then screened to remove contaminants (see G.A.
  • Fibers from different sources are "refined" in order to increase the
  • the fibers are then diluted with water to a low consistency (0.2%-1.5%
  • additives are added to improve paper quality or to improve paper machine
  • the water is then further removed from the sheet. While the drying
  • the sheet surface may be coated (size-press) (see G.A. Smook, ibid, Chapter 18, Surface Treatments, Paragraph 18.1, Surface
  • a BCA containing a CBD may be added, as this provides
  • the refining is done under high intensity conditions that may
  • stage (d) is best for applications that require mainly improved surface

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Textile Engineering (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Materials Engineering (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

L'invention concerne un produit qui renferme un polymère d'hydrates de carbone, tel qu'un produit en papier doté de propriété mécanique améliorée, et qui contient un substrat d'hydrates de carbone qui est un substrat d'hydrates de carbone à base de cellulose réticulé avec un agent de réticulation biologique (BCA) contenant un domaine de liaison de cellulose (CBD) ou lié à un groupe caractéristique de fonctionnalité contenant un domaine de liaison à la cellulose (CBD). La propriété mécanique peut être la résistance à la traction, la résistance à l'état humide, la résistance à l'éclatement, la résistance au déchirement, la résistance d'écrasement à l'anneau, la résistance de compression de courte portée et le frottement humide.
PCT/IL2000/000775 1999-11-25 2000-11-20 Produits en papier renfermant un agent de reticulation biologique WO2001038637A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU15467/01A AU1546701A (en) 1999-11-25 2000-11-20 Paper products comprising a biological cross-linking agent

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL13313499A IL133134A0 (en) 1999-11-25 1999-11-25 Improved paper products
IL133134 1999-11-25

Publications (1)

Publication Number Publication Date
WO2001038637A1 true WO2001038637A1 (fr) 2001-05-31

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PCT/IL2000/000775 WO2001038637A1 (fr) 1999-11-25 2000-11-20 Produits en papier renfermant un agent de reticulation biologique

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IL (1) IL133134A0 (fr)
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009043854A1 (fr) * 2007-10-01 2009-04-09 Novozymes A/S Polypeptides dotés d'une activité de protéase et polynucléotides codant ceux-ci
JP2015144610A (ja) * 2007-11-26 2015-08-13 イッサム リサーチ ディベロップメント カンパニー オブ ザ ヘブリュー ユニバーシティー オブ エルサレム リミテッド 繊維状ポリペプチドおよび多糖を含む組成物
WO2018200460A1 (fr) * 2017-04-24 2018-11-01 Structured I, Llc Procédé de réduction de peluches de tissus et de serviettes
KR20190122120A (ko) * 2017-10-18 2019-10-29 울산대학교 산학협력단 동형2기능성 이미도에스터를 이용한 병원체 농축 방법

Citations (8)

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US5202247A (en) * 1988-07-08 1993-04-13 University Of British Columbia Cellulose binding fusion proteins having a substrate binding region of cellulase
WO1997007203A1 (fr) * 1995-08-16 1997-02-27 Hercules Incorporated Procedes et composes chimiques permettant de modifier des polymeres
WO1998018905A1 (fr) * 1996-10-28 1998-05-07 Novo Nordisk A/S Expression extracellulaire de domaines de fixation de cellulose (cbd) au moyen de bacillus
US5837814A (en) * 1993-04-14 1998-11-17 Yissum Research Development Co. Of Hebrew University Of Jeruslame Cellulose binding domain proteins
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US10883228B2 (en) 2017-04-24 2021-01-05 Structured I, Llc Process for reducing lint from tissue and towel products
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KR102136696B1 (ko) 2017-10-18 2020-07-22 주식회사 인퓨전텍 동형2기능성 이미도에스터를 이용한 병원체 농축 방법
US11753636B2 (en) 2017-10-18 2023-09-12 Infusion Tech Method for enriching pathogen, using homobifunctional imidoester

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