WO2001035068A1 - Dispositif de depot, du type a anneau et a tige, destine a une solution echantillon, - Google Patents

Dispositif de depot, du type a anneau et a tige, destine a une solution echantillon, Download PDF

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Publication number
WO2001035068A1
WO2001035068A1 PCT/JP2000/007908 JP0007908W WO0135068A1 WO 2001035068 A1 WO2001035068 A1 WO 2001035068A1 JP 0007908 W JP0007908 W JP 0007908W WO 0135068 A1 WO0135068 A1 WO 0135068A1
Authority
WO
WIPO (PCT)
Prior art keywords
pin
ring
sample solution
vertical arm
sample
Prior art date
Application number
PCT/JP2000/007908
Other languages
English (en)
Japanese (ja)
Inventor
Junichi Mineno
Haruhisa Sawaragi
Yoshiteru Kora
Minoru Ueda
Masanori Takayama
Kiyozo Asada
Ikunoshin Kato
Original Assignee
Takara Bio Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc. filed Critical Takara Bio Inc.
Priority to KR1020027005493A priority Critical patent/KR20020064890A/ko
Priority to AU13049/01A priority patent/AU1304901A/en
Publication of WO2001035068A1 publication Critical patent/WO2001035068A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0244—Drop counters; Drop formers using pins
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0244—Drop counters; Drop formers using pins
    • B01L3/0251—Pin and ring type or pin in tube type dispenser
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00387—Applications using probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Definitions

  • the present invention relates to a pin-and-ring type sample solution spotting apparatus for spotting a large number of sample solutions, for example, a DNA solution, on a surface of a substrate such as a glass plate.
  • the spotting apparatus 1 generally includes a solution holding mechanism 2 for holding a predetermined amount of a DNA solution, and a pin mechanism 3 for spotting the solution held in the solution holding mechanism 2 onto a substrate. It is configured.
  • the former solution holding mechanism 2 includes a vertical arm 4 extending in the vertical direction, a ring 5 ′ fixed to the lower end of the vertical arm 4, and a first lifting mechanism 6 for lifting and lowering the vertical arm 4.
  • the ring 5 has, for example, an inner diameter of about 2 mm.
  • the latter pin mechanism 3 is a vertical axis passing through the center of the ring 5.
  • the vertical arm 4 When spotting a DNA solution on a substrate using the spotting apparatus 1, first, the vertical arm 4 is lowered by the first lifting mechanism 6, and the ring 5 is immersed in the DNA solution 9 (step (a) in FIG. 4). (See (b)). Thereafter, the vertical arm 4 is raised by the first lifting mechanism 6, and the ring 5 is removed from the DNA solution 9 (see step (c) in FIG. 4). With the ring 5 removed from the DNA solution 9, A predetermined amount of the DNA solution 9 is held in the space inside the ring 5 by the surface tension of the DNA solution 9. In the steps (a) to (c) so far, the pin 7 of the pin mechanism 3 has the ring 5 held at the raised position, and the pin 7 is kept out of contact with the DNA solution 9.
  • the spotting device 1 is moved onto the base 10 (see step (d) in FIG. 4), and the pin 7 is lowered by the second lifting mechanism 8.
  • the pin 7 penetrates the DNA solution 9 held on the ring 5, and at this time, a small amount of the DNA solution 9 having a substantially constant amount is held at the lower end of the pin 7 (step (e) in FIG. 4). See).
  • the pin 7 is further lowered by the second lifting / lowering mechanism 8 so that the lower end of the pin 7 comes into contact with the base 10, and a small amount of the DNA solution 9 held at the lower end of the pin 7 is transferred to the base 1. (See step (f) in Fig. 4).
  • the pin 7 is raised by the second lifting mechanism 8, returns to the raised position shown in FIG. 4D again, and waits until the same DNA solution 9 is spotted on another substrate.
  • the DNA solution 9 that has entered the gap between the pin 7 and the vertical arm 4 rises by capillary action, and as a result, valuable DNA remains in the internal space of the ring 5. In some cases, the amount of the DNA solution 9 was substantially reduced so that the desired number of spottings could not be performed.
  • the DNA solution 9 remaining in the vertical arm 4 due to the capillary action may be mixed into another DNA solution held in the ring 5 later.
  • the vertical arms 4 etc. are usually washed after the required number of spottings are completed, so not all of the DNA solution that has risen along the vertical arm 4 will be mixed into another DNA solution. In some cases, the washing may be incomplete. In such a case, it is highly likely that the remaining DNA solution is newly added to the ring or mixed into the DNA solution.
  • the present invention provides a pin-and-ring type sample solution spotting apparatus in which a precious sample solution held in a ring is not lost by capillary action.
  • the purpose is to provide.
  • a pin-and-ring type sample solution spotting apparatus is provided with a lower end portion of a vertical arm adjacent to a ring and the vertical arm when the pin is in the lowered position.
  • a vertical gap of at least 0.3 mm or a vertical length of 5 mm is formed between the lower end of the arm and the lower end of the pin facing the lower end of the arm. It is characterized in that the size of the arm and the pin is determined.
  • a lower end portion of the vertical arm has a small cross section having a small horizontal cross section at a position adjacent to the ring.
  • the vertical arm and the ring are formed by cutting a tube having the same cross section as the ring, and a lower end of the vertical arm facing a lower end portion of the pin The portion has a plane parallel to a vertical plane that contacts the inner surface of the tube.
  • FIG. 1 (a) is a front view of a vertical arm and a ring used in a pin-and-ring type sample solution spotting apparatus according to the present invention.
  • FIG. 1 (b) is a side view of a vertical arm and a ring used in the sample solution spotting apparatus according to the pin & ring method according to the present invention.
  • FIG. 1 (c) is an end view of a vertical arm and a ring used in the sample solution spotting apparatus according to the pin & ring method according to the present invention.
  • FIGS. 1 (d) and 1 (e) are front views of a vertical arm and a ring used in a sample solution spotting apparatus according to the present invention, respectively.
  • FIG. 2 is a front view of a pin used in a sample solution spotting apparatus using a pin and ring method according to the present invention.
  • Figures 3 (a) and (b) show the use of the vertical arm and ring of Figure 1 and the pins shown in Figure 2. It is a figure showing a state.
  • FIGS. 4 (a) to 4 (g) are diagrams illustrating a sample solution spotting apparatus using a pin and ring method and a method of using the same.
  • the sample solution spotting apparatus of the pin & ring type according to the present invention is characterized by a vertical arm 4 and a ring 5 of a solution holding mechanism 2 and a pin 7 of a pin mechanism 3. It is the same as the spotting device described above. Therefore, in the following description, only the configuration of the vertical arm 4, the ring 5, and the pin 7 will be described, and the overall configuration and operation of the spotting device will not be referred to.
  • FIG. 1 (a) to 1 (e) show enlarged views of the vertical arm 4 of the spotting device.
  • the vertical arm 4 and the ring 5 are formed by cutting a single straight stainless steel tube 11.
  • the machined vertical arm 4 moves from one end (upper end) to the other end (lower end) with respect to the central axis 12 of the tube 11 (Fig. 1
  • the middle section 14 where most of the cross section of the pipe is cut off at the plane parallel to the pipe, and the cross section of most of the pipe section at the plane parallel to the central axis 12 of the pipe as shown in Fig. 1 (e). It has a lower end portion 15 having a smaller cross section than the intermediate portion 14.
  • the cross section of the portion 14 ′ cut off to form the intermediate portion 14 is at least half of the tube cross section.
  • the surface 16 facing the central axis 12 is a tube. It is formed by a plane parallel to the surface that is in contact with the inner surface of 11.
  • the surface 16 may be a plane that is in contact with the inner surface of the tube 11 described above (in other words, a plane that includes a tangent to the inner surface of the tube).
  • the lower end portion 15 has a reduced cross section 17 formed by cutting out a portion adjacent to the ring 5 from both sides.
  • This reduced cross-section 17 is perpendicular to the reduced cross-section 17.
  • Various shapes for example, a shape in which a notch is formed from only one of the left side and the right side of one of FIG. 1 (a)
  • the size of the ring 5 in the direction of the central axis 12 is determined so that a required amount of the sample solution can be held in the internal space.
  • FIG. 2 shows an enlarged view of pin 7.
  • the pin 7 is formed by processing a rod made of stainless steel, and moves from one end (upper end) to the other end (lower end) with respect to the center axis 20 of the pin 7 (see FIG. 2). From top to bottom), upper end gripper (first part) 2 1, upper base (second part) 2 2, taper part (third part) 2
  • lower base (fourth part) 24, small diameter part (fifth part) 25, extra small diameter part (sixth part) 26, minimum diameter part (seventh part) 27 I have.
  • the lower end surface of the minimum diameter portion 27 is formed with fine irregularities by blasting using ceramic particles having a diameter of about 30 m so that a predetermined amount of the sample solution can be reliably held on the lower end surface. I have.
  • the vertical arm 4 and the ring 5 configured as described above and the pin 7 are connected to the center axis 12 of the vertical arm 4 and the ring 5 by the center of the pin 7.
  • L fixed to the first and second elevating mechanisms 6, 8 (see FIG. 4) with the axis 20 aligned.
  • the pins 7 are penetrated into the DNA solution in the ring 5, and at this time, the minimum diameter
  • the sample solution held on the lower end face of part 27 is spotted on the substrate.
  • the dimensions of the vertical arm 4 and the pin 7 and especially the size of the lower end 15 of the vertical arm 4 and the extra small diameter part 26 of the pin 7 are as shown in Fig. 3.
  • a space 28 of a predetermined size is formed between the lower end portion 15 and the space 28 so as to prevent the sample solution from rising along the vertical arm in the space 28 due to capillary action.
  • the rise was slight, and there was no practical problem. Also, when the horizontal size of the space 28 is 0.3 mm and the vertical length of the ultra-small diameter portion 26 (the vertical size of the space 28) is 5 mm or more, the sample solution due to the capillary phenomenon is generated. The rise has further declined. In the most preferable combination, when the distance between the micro-diameter portion 26 and the vertical arm 4 is 0.45 mm or more and the vertical length of the micro-diameter portion 26 is 7.5 mm or more, the sample solution due to the capillary phenomenon is obtained. Was almost completely suppressed. It was also confirmed that the reduced cross-section 17 formed at the lower end 15 of the vertical arm 4 was effective in suppressing the rise of the sample solution due to capillary action.
  • the distance between the very small diameter portion 26 and the vertical arm 4 is 0.3 mm or more, preferably 0.45 mm or more, and the vertical length of the very small diameter portion 26 is 5 mm or more, preferably 7.5. It can be understood that it should be at least mm.
  • the substrate on which the sample solution is spotted using the spotting apparatus of the present invention is not particularly limited as long as it is a material that is practically insoluble, and examples thereof include paper, nylon, cellulose, and nitrocellulose. Plastics and plastic derivatives such as cellulose derivatives, polycarbonate, polystyrene and polypropylene, magnetic or non-magnetic metals, quartz and glass and the like are included.
  • the shape is not particularly limited as long as the sample solution can be spotted by the above-mentioned device, but a plate having a uniform thickness or a substrate on a film is suitable for using the device of the present invention. You.
  • the sample solution spotted using the spotting apparatus of the present invention is not particularly limited, and may be a solution containing any sample desired to be spotted on a substrate.
  • the device of the present invention is particularly suitable for immobilizing biological materials in the fields of biochemistry, molecular biology and the like.
  • the sample solution used in the operation include a solution containing a biologically-related material such as a nucleic acid, a protein, a peptide, a carbohydrate, and a drug.
  • the substrate on which such a biological material is immobilized is useful for searching for and detecting a substance having an affinity for the material.
  • the vertical arm, ring, and pin are each made of stainless steel.
  • the pipes and rods were formed by processing, respectively, but the material constituting these is not limited to stainless steel, and may be formed of non-ferrous metals, non-metals including plastics and ceramics.
  • the pin-and-ring type sample solution spotting apparatus enables spotless precious sample solutions held in the ring to be spotted on the substrate without use.
  • the spot size of the sample solution spotted on the substrate has a substantially constant size and shape. Furthermore, since the sample solution does not rise to the vertical arm and remains there due to the capillary action, another DNA solution does not mix with the sample solution held in the ring.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Ce dispositif de dépôt (1), du type à anneau et à tige, destiné à une solution échantillon, peut déposer une solution échantillon précieuse, sans perte, sur un substrat, et il est caractérisé en ce qu'un espace (28) se forme entre une portion d'extrémité inférieure (15) d'un segment vertical (4) adjacent à un anneau (5) et une portion d'extrémité inférieure (26) d'une tige (7), opposée à la portion d'extrémité inférieure (15) du segment vertical (4), lorsque la tige (7) est placée au niveau d'une position abaissée, ledit espace (28) possédant, horizontalement, une longueur de l'ordre de 0,3 mm ou plus, ou verticalement, une longueur de 5 m ou davantage.
PCT/JP2000/007908 1999-11-10 2000-11-10 Dispositif de depot, du type a anneau et a tige, destine a une solution echantillon, WO2001035068A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
KR1020027005493A KR20020064890A (ko) 1999-11-10 2000-11-10 핀 및 링 방식에 의한 시료 용액의 스폿팅 장치
AU13049/01A AU1304901A (en) 1999-11-10 2000-11-10 Pin and ring type spotting device for sample solution

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP11/319500 1999-11-10
JP31950099 1999-11-10

Publications (1)

Publication Number Publication Date
WO2001035068A1 true WO2001035068A1 (fr) 2001-05-17

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ID=18110925

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2000/007908 WO2001035068A1 (fr) 1999-11-10 2000-11-10 Dispositif de depot, du type a anneau et a tige, destine a une solution echantillon,

Country Status (4)

Country Link
KR (1) KR20020064890A (fr)
CN (1) CN1390300A (fr)
AU (1) AU1304901A (fr)
WO (1) WO2001035068A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004089530A1 (fr) * 2003-04-10 2004-10-21 Infineon Technologies Ag Dispositif permettant l'application sans dommage de biomolecules en solution sur des substrats sensibles
US7160496B2 (en) 2002-11-01 2007-01-09 Delphi Technologies, Inc. Thermoplastic compositions containing metal material
WO2022050399A1 (fr) * 2020-09-03 2022-03-10 セルスペクト株式会社 Instrument de collecte de liquide, procédé de fabrication d'un instrument de collecte de liquide et kit d'inspection

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100685892B1 (ko) 2005-06-07 2007-02-26 동부일렉트로닉스 주식회사 씨모스 이미지 센서 및 그 제조방법

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997046319A1 (fr) * 1996-06-05 1997-12-11 Molecular Dynamics, Inc. Dispositif de formation tres rapide d'un depot de liquide en vue de la formation de matrices de molecules biologiques
JP2000287670A (ja) * 1999-02-03 2000-10-17 Kaken Kogyo:Kk 液の転写部材及び転写装置

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997046319A1 (fr) * 1996-06-05 1997-12-11 Molecular Dynamics, Inc. Dispositif de formation tres rapide d'un depot de liquide en vue de la formation de matrices de molecules biologiques
JP2000287670A (ja) * 1999-02-03 2000-10-17 Kaken Kogyo:Kk 液の転写部材及び転写装置

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7160496B2 (en) 2002-11-01 2007-01-09 Delphi Technologies, Inc. Thermoplastic compositions containing metal material
WO2004089530A1 (fr) * 2003-04-10 2004-10-21 Infineon Technologies Ag Dispositif permettant l'application sans dommage de biomolecules en solution sur des substrats sensibles
DE10316580A1 (de) * 2003-04-10 2004-11-04 Infineon Technologies Ag Vorrichtung zum beschädigungsfreien Aufbringen von Bio-Molekülen in Lösung auf empfindlichen Substraten
WO2022050399A1 (fr) * 2020-09-03 2022-03-10 セルスペクト株式会社 Instrument de collecte de liquide, procédé de fabrication d'un instrument de collecte de liquide et kit d'inspection

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KR20020064890A (ko) 2002-08-10
AU1304901A (en) 2001-06-06
CN1390300A (zh) 2003-01-08

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