WO2001021838A3 - Systeme tridimensionnel de micro-reseau servant au genotypage en parallele du polymorphisme de nucleotides isoles - Google Patents

Systeme tridimensionnel de micro-reseau servant au genotypage en parallele du polymorphisme de nucleotides isoles Download PDF

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Publication number
WO2001021838A3
WO2001021838A3 PCT/US2000/025988 US0025988W WO0121838A3 WO 2001021838 A3 WO2001021838 A3 WO 2001021838A3 US 0025988 W US0025988 W US 0025988W WO 0121838 A3 WO0121838 A3 WO 0121838A3
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WO
WIPO (PCT)
Prior art keywords
single nucleotide
nucleotide polymorphisms
microarray system
dimensional microarray
parallel genotyping
Prior art date
Application number
PCT/US2000/025988
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English (en)
Other versions
WO2001021838A2 (fr
Inventor
Walter Klimecki
Original Assignee
Motorola Inc
Walter Klimecki
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Motorola Inc, Walter Klimecki filed Critical Motorola Inc
Priority to JP2001525396A priority Critical patent/JP2003510054A/ja
Priority to EP00966797A priority patent/EP1222310A2/fr
Priority to CA002385851A priority patent/CA2385851A1/fr
Priority to AU77085/00A priority patent/AU7708500A/en
Publication of WO2001021838A2 publication Critical patent/WO2001021838A2/fr
Publication of WO2001021838A3 publication Critical patent/WO2001021838A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B01J2219/00587—High throughput processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00644—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being present in discrete locations, e.g. gel pads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00664—Three-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Abstract

L'invention porte sur des méthodes de détection des mutations d'acide nucléique et de polymorphismes génétiques par analyse de l'extension d'une base isolée, et spécifiquement sur un appareil et des procédés de détection de l'extension d'une base isolée d'un oligonucléotide particulier d'un micro-réseau d'oligonucléotides suite à une hybridation entre des oligonucléotides fixés à des régions définies d'un micro-réseau polymérique à base d'hydrogel et aux acides nucléiques d'un échantillon biologique.
PCT/US2000/025988 1999-09-22 2000-09-22 Systeme tridimensionnel de micro-reseau servant au genotypage en parallele du polymorphisme de nucleotides isoles WO2001021838A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2001525396A JP2003510054A (ja) 1999-09-22 2000-09-22 単一ヌクレオチド多形性の平行遺伝子型決定のための三次元ミクロアレイシステム
EP00966797A EP1222310A2 (fr) 1999-09-22 2000-09-22 Systeme tridimensionnel de micro-reseau servant au genotypage en parallele du polymorphisme de nucleotides isoles
CA002385851A CA2385851A1 (fr) 1999-09-22 2000-09-22 Systeme tridimensionnel de micro-reseau servant au genotypage en parallele du polymorphisme de nucleotides isoles
AU77085/00A AU7708500A (en) 1999-09-22 2000-09-22 Three-dimensional microarray system for parallel genotyping of single nucleotide polymorphisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15543199P 1999-09-22 1999-09-22
US60/155,431 1999-09-22

Publications (2)

Publication Number Publication Date
WO2001021838A2 WO2001021838A2 (fr) 2001-03-29
WO2001021838A3 true WO2001021838A3 (fr) 2001-10-25

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PCT/US2000/025988 WO2001021838A2 (fr) 1999-09-22 2000-09-22 Systeme tridimensionnel de micro-reseau servant au genotypage en parallele du polymorphisme de nucleotides isoles

Country Status (5)

Country Link
EP (1) EP1222310A2 (fr)
JP (1) JP2003510054A (fr)
AU (1) AU7708500A (fr)
CA (1) CA2385851A1 (fr)
WO (1) WO2001021838A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002353997A1 (en) * 2001-11-01 2003-05-12 Rensselaer Polytechnic Institute In vitro metabolic engineering on microscale devices
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008014485A2 (fr) 2006-07-28 2008-01-31 California Institute Of Technology Ensembles de pcr-q multiplex
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US20120141986A1 (en) * 2007-03-27 2012-06-07 Kenneth Kuhn Multivalent substrate elements for detection of nucleic acid sequences
WO2011129710A1 (fr) * 2010-04-15 2011-10-20 Digital Sensing Limited Microréseaux
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017155858A1 (fr) 2016-03-07 2017-09-14 Insilixa, Inc. Identification de séquence d'acide nucléique à l'aide d'une extension de base unique cyclique en phase solide
EP3577233B1 (fr) 2017-03-31 2021-07-14 Marcella Chiari Génotypage de mutations par combinaison d'hybridation en tube et de microréseau d'étiquettes universelles
JP2022525322A (ja) 2019-03-14 2022-05-12 インシリクサ， インコーポレイテッド 時間ゲート蛍光ベースの検出のための方法およびシステム
CN113943324B (zh) * 2021-10-25 2023-07-28 中元汇吉生物技术股份有限公司 一种修饰的核苷酸，组合物及试剂

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990009455A1 (fr) * 1989-02-13 1990-08-23 Geneco Pty Ltd Detection d'une sequence d'acides nucleiques ou d'un changement dans celle-ci
EP0535242A1 (fr) * 1991-03-18 1993-04-07 Institut Molekulyarnoi Biologii Imeni V.A. Engelgardta Akademii Nauk Sssr Procede et dispositif de determination de la sequence de nucleotides d'adn
WO1993025563A1 (fr) * 1992-06-17 1993-12-23 City Of Hope Procede de detection de sequences d'acide nucleique et de discrimination entre ces sequences
US5770721A (en) * 1993-08-11 1998-06-23 University Of Chicago Method of manufacturing a matrix for the detection of mismatches
WO1998028438A1 (fr) * 1996-12-20 1998-07-02 Diatech Pty. Ltd. Procede de detection de mutations ponctuelles dans lequel l'amorce est liee a un support solide et l'extension ne se produit pas si le(s) nucleotide(s) terminal/aux presentent un defaut de complementarite
WO1998044152A1 (fr) * 1997-04-01 1998-10-08 Glaxo Group Limited Methode de sequençage d'acide nucleique
WO1999018242A1 (fr) * 1997-10-07 1999-04-15 Motorola Inc. Capteurs permettant de detecter electriquement les phenomenes de liaison relatifs aux recepteurs moleculaires sur support
WO2000031148A2 (fr) * 1998-11-25 2000-06-02 Motorola, Inc. Hydrogels de polyacrylamide obtenus a partir de prepolymeres reactifs

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990009455A1 (fr) * 1989-02-13 1990-08-23 Geneco Pty Ltd Detection d'une sequence d'acides nucleiques ou d'un changement dans celle-ci
EP0535242A1 (fr) * 1991-03-18 1993-04-07 Institut Molekulyarnoi Biologii Imeni V.A. Engelgardta Akademii Nauk Sssr Procede et dispositif de determination de la sequence de nucleotides d'adn
WO1993025563A1 (fr) * 1992-06-17 1993-12-23 City Of Hope Procede de detection de sequences d'acide nucleique et de discrimination entre ces sequences
US5770721A (en) * 1993-08-11 1998-06-23 University Of Chicago Method of manufacturing a matrix for the detection of mismatches
WO1998028438A1 (fr) * 1996-12-20 1998-07-02 Diatech Pty. Ltd. Procede de detection de mutations ponctuelles dans lequel l'amorce est liee a un support solide et l'extension ne se produit pas si le(s) nucleotide(s) terminal/aux presentent un defaut de complementarite
WO1998044152A1 (fr) * 1997-04-01 1998-10-08 Glaxo Group Limited Methode de sequençage d'acide nucleique
WO1999018242A1 (fr) * 1997-10-07 1999-04-15 Motorola Inc. Capteurs permettant de detecter electriquement les phenomenes de liaison relatifs aux recepteurs moleculaires sur support
WO2000031148A2 (fr) * 1998-11-25 2000-06-02 Motorola, Inc. Hydrogels de polyacrylamide obtenus a partir de prepolymeres reactifs

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Title
DUBILEY ET AL.: "POLYMORPHISM ANALYSIS AND GENE DETECTION BY MINISEQUENCING ON AN ARRAY OF GEL-IMMOBILIZED PRIMERS", NUCLEIC ACIDS RESEARCH/ONLINE, vol. 27, no. 18, August 1999 (1999-08-01), pages e19, XP002153648 *
MCKENZIE STEVEN E ET AL: "Parallel molecular genetic analysis.", EUROPEAN JOURNAL OF HUMAN GENETICS, vol. 6, no. 5, 1998, pages 417 - 429, XP000953351, ISSN: 1018-4813 *
SYVANEN ANN-CHRISTINE: "From gels to chips: "minisequencing" primer extension for analysis of point mutations and single nucleotide polymorphisms.", HUMAN MUTATION, vol. 13, no. 1, 6 April 1999 (1999-04-06), pages 1 - 10, XP000953256, ISSN: 1059-7794 *
YERSHOV G ET AL: "DNA ANALYSIS AND DIAGNOSTICS ON OLIGONUCLEOTIDE MICROCHIPS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 93, 1 May 1996 (1996-05-01), pages 4913 - 4918, XP002064987, ISSN: 0027-8424 *

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Publication number Publication date
AU7708500A (en) 2001-04-24
EP1222310A2 (fr) 2002-07-17
WO2001021838A2 (fr) 2001-03-29
CA2385851A1 (fr) 2001-03-29
JP2003510054A (ja) 2003-03-18

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