WO2001020030A3 - Sequences nucleotidiques issues de genes codant pour la trimethylamine n-oxyde reductase, et leurs utilisations, notamment pour la detection de bacteries - Google Patents

Sequences nucleotidiques issues de genes codant pour la trimethylamine n-oxyde reductase, et leurs utilisations, notamment pour la detection de bacteries Download PDF

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Publication number
WO2001020030A3
WO2001020030A3 PCT/FR2000/002578 FR0002578W WO0120030A3 WO 2001020030 A3 WO2001020030 A3 WO 2001020030A3 FR 0002578 W FR0002578 W FR 0002578W WO 0120030 A3 WO0120030 A3 WO 0120030A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide sequences
trimethylamine
bacteria
sequences derived
genes coding
Prior art date
Application number
PCT/FR2000/002578
Other languages
English (en)
Other versions
WO2001020030A2 (fr
Inventor
Gerard Giordano
Santos Jean-Philippe Dos
Vincent Mejean
Original Assignee
Centre Nat Rech Scient
Gerard Giordano
Dos Santos Jean Philippe
Vincent Mejean
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient, Gerard Giordano, Dos Santos Jean Philippe, Vincent Mejean filed Critical Centre Nat Rech Scient
Priority to EP00966193A priority Critical patent/EP1212360A2/fr
Priority to CA002384637A priority patent/CA2384637A1/fr
Priority to JP2001523801A priority patent/JP2003509067A/ja
Priority to AU76669/00A priority patent/AU7666900A/en
Publication of WO2001020030A2 publication Critical patent/WO2001020030A2/fr
Publication of WO2001020030A3 publication Critical patent/WO2001020030A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

La présente invention a pour objet l'utilisation de séquences nucléotidiques choisies parmi celles comprenant une séquence codant pour une protéine du système triméthylamine N-oxyde réductase (TMAO réductase) chez les bactéries, ou un fragment ou une séquence dérivée de cette séquence, pour la mise en oeuvre d'une méthode de détection de la présence de toutes bactéries impliquées dans le processus de dégradation des chairs d'animaux aquatiques, chez un hôte susceptible d'être porteur de telles bactéries. L'invention a également pour objet des séquences nucléotidiques telles que définies ci-dessus, des méthodes de détection susmentionnées ainsi que les trousses pour la mise en oeuvre de ces méthodes.
PCT/FR2000/002578 1999-09-15 2000-09-15 Sequences nucleotidiques issues de genes codant pour la trimethylamine n-oxyde reductase, et leurs utilisations, notamment pour la detection de bacteries WO2001020030A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00966193A EP1212360A2 (fr) 1999-09-15 2000-09-15 Sequences nucleotidiques issues de genes codant pour la trimethylamine n-oxyde reductase, et leurs utilisations, notamment pour la detection de bacteries
CA002384637A CA2384637A1 (fr) 1999-09-15 2000-09-15 Sequences nucleotidiques issues de genes codant pour la trimethylamine n-oxyde reductase, et leurs utilisations, notamment pour la detection de bacteries
JP2001523801A JP2003509067A (ja) 1999-09-15 2000-09-15 トリメチルアミンｎ−オキシドレダクターゼをコードする遺伝子に由来するヌクレオチド配列、および特に細菌検出のためのその使用
AU76669/00A AU7666900A (en) 1999-09-15 2000-09-15 Nucleotide sequences derived from genes coding for trimethylamine n-oxide reductase, uses thereof in particular for detecting bacteria

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9911543A FR2798393B1 (fr) 1999-09-15 1999-09-15 Sequences nucleotidiques issues de genes codant pour la trimethylamine n-oxyde reductase et leurs utilisations, notamment pour la detection de bacteries
FR99/11543 1999-09-15

Publications (2)

Publication Number Publication Date
WO2001020030A2 WO2001020030A2 (fr) 2001-03-22
WO2001020030A3 true WO2001020030A3 (fr) 2001-11-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2000/002578 WO2001020030A2 (fr) 1999-09-15 2000-09-15 Sequences nucleotidiques issues de genes codant pour la trimethylamine n-oxyde reductase, et leurs utilisations, notamment pour la detection de bacteries

Country Status (6)

Country Link
EP (1) EP1212360A2 (fr)
JP (1) JP2003509067A (fr)
AU (1) AU7666900A (fr)
CA (1) CA2384637A1 (fr)
FR (1) FR2798393B1 (fr)
WO (1) WO2001020030A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6632632B1 (en) * 2001-12-14 2003-10-14 Colifast As Rapid method of detection and enumeration of sulfide-producing bacteria in food products
US7811783B2 (en) 2001-12-14 2010-10-12 Colifast As Rapid method of detection and enumeration of sulfide-producing bacteria in food products
CN105525019B (zh) * 2016-01-31 2019-04-09 浙江工商大学 荧光定量pcr对鲐鱼中腐败微生物的检测法及所用引物
CN110616163B (zh) * 2018-06-20 2021-07-02 南方医科大学珠江医院 能够降低三甲胺浓度的粪肠球菌及其制备方法和应用

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CHEN S ET AL: "A rapid, sensitive and automated method for detection of Salmonella species in food using AG-9600 AmpliSensor analyzer", JOURNAL OF APPLIED MICROBIOLOGY,GB,OXFORD, vol. 83, no. 3, September 1997 (1997-09-01), pages 314 - 321, XP002099375, ISSN: 1364-5072 *
DALGAARD P: "QUALITATIVE AND QUANTITATIVE CHARACTERIZATION OF SPOILAGE BACTERIA FROM PACKED FISH", INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM,NL, vol. 26, no. 3, 1995, pages 319 - 333, XP000929951, ISSN: 0168-1605 *
GRAM L ET AL: "MICROBIOLOGICAL SPOILAGE OF FISH AND FISH PRODUCTS", INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM,NL, vol. 33, 1996, pages 121 - 137, XP000929949, ISSN: 0168-1605 *
JOURLIN ET AL: "CONSERVATION OF CIS-ACTING ELEMENTS WITHIN THE TOR REGULATORY REGION AMONG DIFFERENT ENTEROBACTERIACEAE", GENE,ELSEVIER BIOMEDICAL PRESS. AMSTERDAM,NL, vol. 152, 1995, pages 53 - 57, XP002144796, ISSN: 0378-1119 *
OSORIO ET AL: "16S rRNA GENE SEQUENCE ANALYSIS OF PHOTOBACTERIUM DAMSELAE AND NESTED PCR METHOD FOR RAPID DETECTION OF THE CAUSATIVE AGENT OF FISH PASTEURELLOSIS", APPLIED AND ENVIRONMENTAL MICROBIOLOGY,WASHINGTON,DC,US, vol. 65, no. 7, July 1999 (1999-07-01), pages 2942 - 2946, XP002144797, ISSN: 0099-2240 *
READ S C ET AL: "POLYMERASE CHAIN REACTION FOR DETECTION OF VEROCYTOTOXIGENIC ESCHERICHIA COLI ISOLATED FROM ANIMAL AND FOOD SOURCES", MOLECULAR AND CELLULAR PROBES, 1992, XP002048514 *
SANTOS DOS J P ET AL: "MOLECULAR ANALYSIS OF THE TRIMETHYLAMINE N-OXIDE (TMAO) REDUCTASE RESPIRATORY SYSTEM FROM A SHEWANELLA SPECIES", JOURNAL OF MOLECULAR BIOLOGY,LONDON,GB, vol. 284, no. 2, 1998, pages 421 - 433, XP000929681, ISSN: 0022-2836 *

Also Published As

Publication number Publication date
JP2003509067A (ja) 2003-03-11
AU7666900A (en) 2001-04-17
FR2798393A1 (fr) 2001-03-16
CA2384637A1 (fr) 2001-03-22
EP1212360A2 (fr) 2002-06-12
FR2798393B1 (fr) 2005-07-29
WO2001020030A2 (fr) 2001-03-22

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