WO2001009352A3 - Use of aspergillus sojae as host for recombinant protein production - Google Patents

Use of aspergillus sojae as host for recombinant protein production Download PDF

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Publication number
WO2001009352A3
WO2001009352A3 PCT/NL2000/000544 NL0000544W WO0109352A3 WO 2001009352 A3 WO2001009352 A3 WO 2001009352A3 NL 0000544 W NL0000544 W NL 0000544W WO 0109352 A3 WO0109352 A3 WO 0109352A3
Authority
WO
WIPO (PCT)
Prior art keywords
aspergillus sojae
exhibiting
fluoro
orotic acid
phytase
Prior art date
Application number
PCT/NL2000/000544
Other languages
French (fr)
Other versions
WO2001009352A2 (en
Inventor
Margreet Heerikhuisen
Den Hondel Cornelis Van
Peter Punt
Biezen Nick Van
Alwin Albers
Kurt Vogel
Original Assignee
Tno
Hoffmann La Roche
Margreet Heerikhuisen
Den Hondel Cornelis Van
Peter Punt
Biezen Nick Van
Alwin Albers
Kurt Vogel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tno, Hoffmann La Roche, Margreet Heerikhuisen, Den Hondel Cornelis Van, Peter Punt, Biezen Nick Van, Alwin Albers, Kurt Vogel filed Critical Tno
Priority to KR1020027001321A priority Critical patent/KR20020059350A/en
Priority to AU63238/00A priority patent/AU782116B2/en
Priority to EP00950088A priority patent/EP1204758A2/en
Priority to CA002378016A priority patent/CA2378016A1/en
Priority to JP2001514144A priority patent/JP2003518921A/en
Priority to BR0012856-2A priority patent/BR0012856A/en
Priority to MXPA02001061A priority patent/MXPA02001061A/en
Publication of WO2001009352A2 publication Critical patent/WO2001009352A2/en
Publication of WO2001009352A3 publication Critical patent/WO2001009352A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A recombinant Aspergillus sojae comprising an introduced acetamidase S (amdS) gene as selectable marker is disclosed. An Aspergillus sojae exhibiting growth with medium comprising uracil and fluoro-orotic acid, said Aspergillus sojae further not exhibiting growth on medium comprising uridine and fluoro-orotic acid i.e. said Aspergillus sojae exhibiting uracil auxotrophy, said Aspergillus sojae being unable to utilize uridine, said Aspergillus sojae being pyrG negative, said Aspergillus sojae exhibiting resistance to fluoro-orotic acid, said uracil auxotrophy and said fluoro-orotic acid resistance being relievable upon complementation with an active introduced pyrG gene, is described. The Aspergillus sojae further comprises a nucleic acid sequence encoding a phytase or a protein having phytase activity or any other heterologous protein or polypeptide and can be used for the biotechnological production of said phytase or said other heterologous proteins or polypeptides. Additional mutants exhibiting amended morphology are also disclosed. Methods of producing such expression hosts are described.
PCT/NL2000/000544 1999-07-30 2000-07-28 Use of aspergillus sojae as host for recombinant protein production WO2001009352A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
KR1020027001321A KR20020059350A (en) 1999-07-30 2000-07-28 Use of aspergillus sojae as host for recombinant protein producion
AU63238/00A AU782116B2 (en) 1999-07-30 2000-07-28 Novel means of transformation of fungi and their use for heterologous protein production
EP00950088A EP1204758A2 (en) 1999-07-30 2000-07-28 Use of aspergillus sojae as host for recombinant protein production
CA002378016A CA2378016A1 (en) 1999-07-30 2000-07-28 Use of aspergillus sojae as host for recombinant protein production
JP2001514144A JP2003518921A (en) 1999-07-30 2000-07-28 Use of Aspergillus sojae as a host for recombinant protein production
BR0012856-2A BR0012856A (en) 1999-07-30 2000-07-28 Recombinant Aspergillus soybean, methods for introducing a nucleic acid sequence into aspergillus soybean, to select transformed or transfected aspergillus soybean, to produce recombinant aspergillus soybean, mutant or recombinant aspergillus soybean, recombinant fungus, expression of an introduced nucleic acid sequence, for the expression of a protein or polypeptide, for the production of a protein or polypeptide, and, to produce a phytase or a protein having phytase activity
MXPA02001061A MXPA02001061A (en) 1999-07-30 2000-07-28 Use of aspergillus.

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP99202516 1999-07-30
EP99202517 1999-07-30
EP99202517.1 1999-07-30
EP99202516.3 1999-07-30

Publications (2)

Publication Number Publication Date
WO2001009352A2 WO2001009352A2 (en) 2001-02-08
WO2001009352A3 true WO2001009352A3 (en) 2001-11-08

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2000/000544 WO2001009352A2 (en) 1999-07-30 2000-07-28 Use of aspergillus sojae as host for recombinant protein production

Country Status (10)

Country Link
EP (1) EP1204758A2 (en)
JP (1) JP2003518921A (en)
KR (1) KR20020059350A (en)
CN (1) CN1369016A (en)
AR (1) AR024989A1 (en)
AU (1) AU782116B2 (en)
BR (1) BR0012856A (en)
CA (1) CA2378016A1 (en)
MX (1) MXPA02001061A (en)
WO (1) WO2001009352A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101993939B1 (en) 2011-04-22 2019-06-27 다니스코 유에스 인크. Filamentous fungi having an altered viscosity phenotype

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
KR20020026456A (en) 2000-04-13 2002-04-10 에말파브 마크 아론 High-throughput screening of expressed dna libraries in filamentous fungi
WO2009033071A2 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
JP2011239681A (en) * 2008-09-19 2011-12-01 Kikkoman Corp Koji mold alkaline protease promoter
CN101503660B (en) * 2009-02-10 2011-07-27 汪兵 Engineering bacteria expressing thermophilic saccharifying enzyme and use thereof
CA2833539C (en) * 2011-04-22 2021-04-27 Danisco Us Inc. Filamentous fungi having an altered viscosity phenotype

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02234666A (en) * 1989-03-09 1990-09-17 Kikkoman Corp Aspergillus-sojae transformant
WO1997004108A1 (en) * 1995-07-20 1997-02-06 Röhm Gmbh Leucine aminopeptidases produced recombinantly from aspergillus soyae
EP0758020A2 (en) * 1995-08-03 1997-02-12 Gist-Brocades B.V. The use of homologous amdS genes as selectable markers
EP0967286A2 (en) * 1998-06-12 1999-12-29 Kikkoman Corporation Leucine aminopeptidase gene, recombinant dna, and process for producing leucine aminopeptidase

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02234666A (en) * 1989-03-09 1990-09-17 Kikkoman Corp Aspergillus-sojae transformant
WO1997004108A1 (en) * 1995-07-20 1997-02-06 Röhm Gmbh Leucine aminopeptidases produced recombinantly from aspergillus soyae
EP0758020A2 (en) * 1995-08-03 1997-02-12 Gist-Brocades B.V. The use of homologous amdS genes as selectable markers
EP0967286A2 (en) * 1998-06-12 1999-12-29 Kikkoman Corporation Leucine aminopeptidase gene, recombinant dna, and process for producing leucine aminopeptidase

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BROEKHUIJSEN M P ET AL: "Secretion of heterologous proteins by Aspergillus niger: Production of active human interleukin-6 in a protease-deficient mutant by KEX2-like processing of a glucoamylase-hIL6 fusion protein.", JOURNAL OF BIOTECHNOLOGY, vol. 31, no. 2, 1993, pages 135 - 145, XP002167253, ISSN: 0168-1656 *
D'ENFERT CHRISTOPHE: "Selection of multiple disruption events in Aspergillus fumigatus using the orotidine-5'-decarboxylase gene, pyrG, as a unique transformation marker.", CURRENT GENETICS, vol. 30, no. 1, 1996, pages 76 - 82, XP002107254, ISSN: 0172-8083 *
ENDERLIN CAROL S ET AL: "Cloning, nucleotide sequence and functions of XP R6, which codes for a dibasic processing endoprotease from the yeast Yarrowia lipolytica.", YEAST, vol. 10, no. 1, 1994, pages 67 - 79, XP000993444, ISSN: 0749-503X *
GOMI K ET AL: "TRANSFORMATION OF THE INDUSTRIAL STRAIN OF ASPERGILLUS ORYZAE WITH THE HOMOLOGOUS AMDS GENE AS A DOMINANT SELECTABLE MARKER", JOURNAL OF FERMENTATION AND BIOENGINEERING,JP,SOCIETY OF FERMENTATION TECHNOLOGY, vol. 74, no. 6, 1992, pages 389 - 391, XP000609456, ISSN: 0922-338X *
HATAMOTO OSAMU ET AL: "Cloning and expression of a cDNA encoding the laccase from Schizophyllum commune.", BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 63, no. 1, January 1999 (1999-01-01), pages 58 - 64, XP002160357, ISSN: 0916-8451 *
JALVING RUUD ET AL: "Characterization of the kexin-like maturase of Aspergillus niger.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 66, no. 1, January 2000 (2000-01-01), pages 363 - 368, XP002167252, ISSN: 0099-2240 *
PATENT ABSTRACTS OF JAPAN vol. 014, no. 548 (C - 0785) 5 December 1990 (1990-12-05) *
UNKLES S E ET AL: "THE DEVELOPMENT OF A HOMOLOGOUS TRANSFORMATION SYSTEM FOR ASPERGILLUS ORYZAE BASED ON THE NITRATE ASSIMILATION PATHWAY: A CONVENIENT AND GENERAL SELECTION SYSTEM FOR FILAMENTOUS FUNGAL TRANSFORMATION", MOLECULAR AND GENERAL GENETICS,DE,SPRINGER VERLAG, BERLIN, vol. 218, no. 1, 1 July 1989 (1989-07-01), pages 99 - 104, XP000025322, ISSN: 0026-8925 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101993939B1 (en) 2011-04-22 2019-06-27 다니스코 유에스 인크. Filamentous fungi having an altered viscosity phenotype

Also Published As

Publication number Publication date
AU6323800A (en) 2001-02-19
AR024989A1 (en) 2002-11-06
MXPA02001061A (en) 2003-04-10
WO2001009352A2 (en) 2001-02-08
CA2378016A1 (en) 2001-02-08
KR20020059350A (en) 2002-07-12
CN1369016A (en) 2002-09-11
AU782116B2 (en) 2005-07-07
JP2003518921A (en) 2003-06-17
BR0012856A (en) 2002-06-11
EP1204758A2 (en) 2002-05-15

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