AR024989A1 - MEANS OF FUNCTION TRANSFORMATION AND USE OF THE SAME FOR THE PRODUCTION OF HETEROLOGICAL PROTEINS. - Google Patents

MEANS OF FUNCTION TRANSFORMATION AND USE OF THE SAME FOR THE PRODUCTION OF HETEROLOGICAL PROTEINS.

Info

Publication number
AR024989A1
AR024989A1 ARP000103931A ARP000103931A AR024989A1 AR 024989 A1 AR024989 A1 AR 024989A1 AR P000103931 A ARP000103931 A AR P000103931A AR P000103931 A ARP000103931 A AR P000103931A AR 024989 A1 AR024989 A1 AR 024989A1
Authority
AR
Argentina
Prior art keywords
aspergillus sojae
strain
fluoro
uracil
phytase
Prior art date
Application number
ARP000103931A
Other languages
Spanish (es)
Original Assignee
Tno
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tno, Hoffmann La Roche filed Critical Tno
Publication of AR024989A1 publication Critical patent/AR024989A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Se describe una cepa de recombinante de Aspergillus sojae que comprende un gen de acetamidasa S (amdS)introducido como marcador seleccionable. Se describeuna cepa de Aspergillus sojae que crece en un medio que contiene uracilo y ácido fluoro-orotico,donde además dicha Aspergillus sojae no crece sobre un medioque contiene uridina y ácido fluoro-orotico, es decir, dicha cepa de Aspergillus sojae presenta auxotrofia por uracilo, siendo dicha Aspergillus sojae incapazde utilizar uridina, donde dicha Aspergillus sojae es pyrG negativa, donde dicha cepa de Aspergillus sojae presenta resistencia al ácido fluoro orotico, dondedicha auxotrofia por uracilo y dicha resistencia al ácido fluoro-orotico se pueden resolver después de complementar con laintroduccion de un gen pyrG activo.Dicha cepa de Aspergillus sojae comprende además una secuencia de ácidos nucleicos que codifica una fitasa o una proteína que presenta actividad fitasa ocualquier otra proteína o polipéptido heterologo y se puede utilizar para la produccion biotecnologica de dicha fitasa o de dichas proteínas o polipéptidosheterologos. También se describen otros mutantes. Se describen los métodos para producir dichos huéspedes de expresion.A recombinant strain of Aspergillus sojae comprising an acetamidase S (amdS) gene introduced as a selectable marker is described. A strain of Aspergillus sojae is described that grows in a medium that contains uracil and fluoro-orotic acid, where in addition said Aspergillus sojae does not grow on a medium that contains uridine and fluoro-orotic acid, that is, said strain of Aspergillus sojae presents auxotrophy by uracil , said Aspergillus sojae being unable to use uridine, where said Aspergillus sojae is pyrG negative, where said Aspergillus sojae strain exhibits resistance to orotic fluoro acid, where uracil auxotrophy can be resolved and said resistance to fluoro-orotic acid can be resolved after supplementing with the introduction of an active pyrG gene. Such strain of Aspergillus sojae further comprises a nucleic acid sequence encoding a phytase or a protein that exhibits phytase activity or any other heterologous protein or polypeptide and can be used for the biotechnological production of said phytase or said proteins or heterologous polypeptides. Other mutants are also described. Methods for producing such expression hosts are described.

ARP000103931A 1999-07-30 2000-07-28 MEANS OF FUNCTION TRANSFORMATION AND USE OF THE SAME FOR THE PRODUCTION OF HETEROLOGICAL PROTEINS. AR024989A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99202516 1999-07-30
EP99202517 1999-07-30

Publications (1)

Publication Number Publication Date
AR024989A1 true AR024989A1 (en) 2002-11-06

Family

ID=26153350

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP000103931A AR024989A1 (en) 1999-07-30 2000-07-28 MEANS OF FUNCTION TRANSFORMATION AND USE OF THE SAME FOR THE PRODUCTION OF HETEROLOGICAL PROTEINS.

Country Status (10)

Country Link
EP (1) EP1204758A2 (en)
JP (1) JP2003518921A (en)
KR (1) KR20020059350A (en)
CN (1) CN1369016A (en)
AR (1) AR024989A1 (en)
AU (1) AU782116B2 (en)
BR (1) BR0012856A (en)
CA (1) CA2378016A1 (en)
MX (1) MXPA02001061A (en)
WO (1) WO2001009352A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
KR20020026456A (en) 2000-04-13 2002-04-10 에말파브 마크 아론 High-throughput screening of expressed dna libraries in filamentous fungi
CA2736661A1 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
JP2011239681A (en) * 2008-09-19 2011-12-01 Kikkoman Corp Koji mold alkaline protease promoter
CN101503660B (en) * 2009-02-10 2011-07-27 汪兵 Engineering bacteria expressing thermophilic saccharifying enzyme and use thereof
EP2699588B1 (en) 2011-04-22 2016-11-30 Danisco US Inc. Filamentous fungi having an altered viscosity phenotype
US9868958B2 (en) * 2011-04-22 2018-01-16 Danisco Us Inc. Filamentous fungi having an altered viscosity phenotype

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2748347B2 (en) * 1989-03-09 1998-05-06 キッコーマン 株式会社 Aspergillus soya transformant
DE19526485A1 (en) * 1995-07-20 1997-01-23 Roehm Gmbh Recombinantly produced leucine aminopeptidase from Aspergillus sojae
US6548285B1 (en) * 1995-08-03 2003-04-15 Dsm N.V. Polynucleotides encoding Aspergillus Niger and Penicillium Chrysogenum acetamidases and methods of use as selectable markers
JP3727780B2 (en) * 1998-06-12 2005-12-14 キッコーマン株式会社 Leucine aminopeptidase gene, recombinant DNA, and method for producing leucine aminopeptidase

Also Published As

Publication number Publication date
CA2378016A1 (en) 2001-02-08
KR20020059350A (en) 2002-07-12
MXPA02001061A (en) 2003-04-10
AU782116B2 (en) 2005-07-07
AU6323800A (en) 2001-02-19
BR0012856A (en) 2002-06-11
EP1204758A2 (en) 2002-05-15
JP2003518921A (en) 2003-06-17
WO2001009352A2 (en) 2001-02-08
CN1369016A (en) 2002-09-11
WO2001009352A3 (en) 2001-11-08

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