WO2000068363A2 - Variantes de l'elastase et substrats - Google Patents

Variantes de l'elastase et substrats Download PDF

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Publication number
WO2000068363A2
WO2000068363A2 PCT/US2000/006692 US0006692W WO0068363A2 WO 2000068363 A2 WO2000068363 A2 WO 2000068363A2 US 0006692 W US0006692 W US 0006692W WO 0068363 A2 WO0068363 A2 WO 0068363A2
Authority
WO
WIPO (PCT)
Prior art keywords
elastase
substrate
variant
residue
amino acid
Prior art date
Application number
PCT/US2000/006692
Other languages
English (en)
Other versions
WO2000068363A3 (fr
Inventor
Paul J. Carter
William Dall'acqua
Maria Rodriques
Original Assignee
Genentech, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech, Inc. filed Critical Genentech, Inc.
Priority to AU49709/00A priority Critical patent/AU4970900A/en
Publication of WO2000068363A2 publication Critical patent/WO2000068363A2/fr
Publication of WO2000068363A3 publication Critical patent/WO2000068363A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6448—Elastases, e.g. pancreatic elastase (3.4.21.36); leukocyte elastase (3.4.31.37)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des variantes de l'élastase présentant une ou plusieurs substitutions d'acides aminés à des positions sélectionnées d'une élastase précurseur. En particulier, un reste d'histidine de site actif, correspondant à l'histidine 43 présente dans l'élastase neutrophile humaine, peut être substitué par un acide aminé présentant un volume inférieur à celui de l'histidine. Dans des modes de réalisation préférés, les variantes de l'élastase selon l'invention présentent un substrat spécifique remarquable pour ce qui est des substrats contenant les restes d'histidine à la position correspondant à deux restes d'acides aminés du côté N-terminal du site de coupure. L'invention concerne également des substrats préférés pour des variantes de l'élastase. Les variantes de l'élastase et les substrats décrits dans la présente invention sont utilisés pour diverses applications thérapeutiques et non thérapeutiques.
PCT/US2000/006692 1999-05-05 2000-05-04 Variantes de l'elastase et substrats WO2000068363A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU49709/00A AU4970900A (en) 1999-05-05 2000-05-04 Elastase variants and substrates

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13264099P 1999-05-05 1999-05-05
US60/132,640 1999-05-05

Publications (2)

Publication Number Publication Date
WO2000068363A2 true WO2000068363A2 (fr) 2000-11-16
WO2000068363A3 WO2000068363A3 (fr) 2001-02-15

Family

ID=22454939

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/006692 WO2000068363A2 (fr) 1999-05-05 2000-05-04 Variantes de l'elastase et substrats

Country Status (2)

Country Link
AU (1) AU4970900A (fr)
WO (1) WO2000068363A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009079220A2 (fr) * 2007-12-04 2009-06-25 Proteon Therapeutics, Inc. Protéines élastases recombinantes et procédés de fabrication et d'utilisation de celles-ci

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0307478A1 (fr) * 1987-03-05 1989-03-22 Toray Industries, Inc. Protease de serine et gene de protease de serine
WO1998024886A1 (fr) * 1996-12-04 1998-06-11 Brigham And Women's Hospital, Inc. Protease issue de mastocytes clivant le fibrogene

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06303984A (ja) * 1993-04-19 1994-11-01 Japan Tobacco Inc 組換えｄｎａ、それによって形質転換された酵母及びそれを用いたアクアライシンｉの製造方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0307478A1 (fr) * 1987-03-05 1989-03-22 Toray Industries, Inc. Protease de serine et gene de protease de serine
WO1998024886A1 (fr) * 1996-12-04 1998-06-11 Brigham And Women's Hospital, Inc. Protease issue de mastocytes clivant le fibrogene

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DALL'ACQUA WILLIAM ET AL: "Elastase substrate specificity tailored through substrate-assisted catalysis and phage display." PROTEIN ENGINEERING, vol. 12, no. 11, November 1999 (1999-11), pages 981-987, XP000960584 ISSN: 0269-2139 *
DAMAK S ET AL: "EXPRESSION OF THE HUMAN NEUTROPHIL ELASTASE GENE IN THE LUNGS OF TRANSGENIC MICE" JOURNAL OF CELLULAR BIOCHEMISTRY. SUPPLEMENT,US,A.R. LISS, NEW YORK, NY, no. SUPPL, 3 April 1992 (1992-04-03), page 40 XP002910419 ISSN: 0733-1959 *
DATABASE WPI Section Ch, Week 199503 Derwent Publications Ltd., London, GB; Class B04, AN 1995-018277 XP002152475 & JP 06 303984 A (JAPAN TOBACCO INC), 1 November 1994 (1994-11-01) *
EDER JORG ET AL: "Folding of subtilisin BPN': Characterization of a folding intermediate." BIOCHEMISTRY, vol. 32, no. 1, 1993, pages 18-26, XP000960578 ISSN: 0006-2960 *
REHEMTULLA A ET AL: "REGULATION OF PACE PROPEPTIDE-PROCESSING ACTIVITY REQUIREMENT FOR A POST-ENDOPLASMIC RETICULUM COMPARTMENT AND AUTOPROTEOLYTIC ACTIVATION" PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 89, no. 17, 1992, pages 8235-8239, XP000960579 1992 ISSN: 0027-8424 *
TAKAHASHI H ET AL: "STRUCTURE OF THE HUMAN NEUTROPHIL ELASTASE GENE" JOURNAL OF BIOLOGICAL CHEMISTRY,US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, vol. 263, no. 29, 15 October 1988 (1988-10-15), pages 14739-14746, XP002911716 ISSN: 0021-9258 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009079220A2 (fr) * 2007-12-04 2009-06-25 Proteon Therapeutics, Inc. Protéines élastases recombinantes et procédés de fabrication et d'utilisation de celles-ci
WO2009079220A3 (fr) * 2007-12-04 2009-08-06 Proteon Therapeutics Inc Protéines élastases recombinantes et procédés de fabrication et d'utilisation de celles-ci
US8501449B2 (en) 2007-12-04 2013-08-06 Proteon Therapeutics, Inc. Recombinant elastase proteins and methods of manufacturing and use thereof
EP2666854A1 (fr) * 2007-12-04 2013-11-27 Proteon Therapeutics, Inc. Protéines élastases recombinantes, procédés de fabrication et leur utilisation
CN101918547B (zh) * 2007-12-04 2015-01-14 普罗特昂治疗公司 重组的弹性蛋白酶蛋白质及其制备方法和用途
US9057060B2 (en) 2007-12-04 2015-06-16 Proteon Therapeutics, Inc. Recombinant elastase proteins and methods of manufacturing and use thereof
CN104711243A (zh) * 2007-12-04 2015-06-17 普罗特昂治疗公司 重组的弹性蛋白酶蛋白质及其制备方法和用途
EP3176262A1 (fr) * 2007-12-04 2017-06-07 Proteon Therapeutics, Inc. Protéines élastases recombinantes, procédés de fabrication et leur utilisation
KR101824350B1 (ko) 2007-12-04 2018-01-31 프로테온 테라퓨틱스, 인코포레이티드 재조합 엘라스타아제 단백질, 및 이의 제조 방법 및 용도
US10301612B2 (en) 2007-12-04 2019-05-28 Proteon Therapeutics, Inc. Recombinant elastase proteins and methods of manufacturing and use thereof
US10308924B2 (en) 2007-12-04 2019-06-04 Proteon Therapeutics, Inc. Nucleic acid molecules encoding autoactivating type I pancreatic proelastase proteins
CN109893647A (zh) * 2007-12-04 2019-06-18 普罗特昂治疗公司 重组的弹性蛋白酶蛋白质及其制备方法和用途

Also Published As

Publication number Publication date
WO2000068363A3 (fr) 2001-02-15
AU4970900A (en) 2000-11-21

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