WO2000065330A1 - Procede d'analyse optique de milieu non homogene - Google Patents
Procede d'analyse optique de milieu non homogene Download PDFInfo
- Publication number
- WO2000065330A1 WO2000065330A1 PCT/JP2000/002638 JP0002638W WO0065330A1 WO 2000065330 A1 WO2000065330 A1 WO 2000065330A1 JP 0002638 W JP0002638 W JP 0002638W WO 0065330 A1 WO0065330 A1 WO 0065330A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- light
- medium
- function
- scattering
- equation
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 61
- 230000006870 function Effects 0.000 claims abstract description 47
- 238000005259 measurement Methods 0.000 claims abstract description 41
- 230000035515 penetration Effects 0.000 claims abstract description 13
- 238000010521 absorption reaction Methods 0.000 claims description 33
- 238000004364 calculation method Methods 0.000 claims description 32
- 238000004458 analytical method Methods 0.000 claims description 23
- 230000003287 optical effect Effects 0.000 claims description 12
- 238000000149 argon plasma sintering Methods 0.000 claims description 11
- 238000005457 optimization Methods 0.000 claims description 7
- 238000004204 optical analysis method Methods 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 5
- 235000012745 brilliant blue FCF Nutrition 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 description 67
- 230000009102 absorption Effects 0.000 description 32
- 239000011159 matrix material Substances 0.000 description 12
- 239000000126 substance Substances 0.000 description 12
- 230000003993 interaction Effects 0.000 description 10
- 238000000862 absorption spectrum Methods 0.000 description 8
- 230000008520 organization Effects 0.000 description 8
- 239000000523 sample Substances 0.000 description 8
- 238000001228 spectrum Methods 0.000 description 8
- 230000005540 biological transmission Effects 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000013076 target substance Substances 0.000 description 5
- 239000013598 vector Substances 0.000 description 5
- 102000001554 Hemoglobins Human genes 0.000 description 4
- 108010054147 Hemoglobins Proteins 0.000 description 4
- 239000000470 constituent Substances 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000003595 spectral effect Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000012937 correction Methods 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 239000010410 layer Substances 0.000 description 3
- 230000031700 light absorption Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 102000018832 Cytochromes Human genes 0.000 description 2
- 108010052832 Cytochromes Proteins 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 210000003323 beak Anatomy 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 238000001028 reflection method Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 238000000342 Monte Carlo simulation Methods 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 238000004422 calculation algorithm Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000003517 fume Substances 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000008241 heterogeneous mixture Substances 0.000 description 1
- 238000012886 linear function Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012067 mathematical method Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000010349 pulsation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000015541 sensory perception of touch Effects 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
Definitions
- the present invention relates to a method for optically analyzing a heterogeneous medium.
- Japanese Patent Application Laid-Open No. H4-297854 is known. This publication discloses a method for correcting a light diffusion effect in a medium, and uses an exponential function as an absorbance correction function.
- the medium when the medium exhibits light scattering and has an inhomogeneous structure, in other words, when the medium is not uniform and is buried in a translucent or milky white medium, a physical quantity analysis method is known. Absent. Natural products and organisms are typical of heterogeneous and dispersive media. Of course, an inhomogeneous medium can be analyzed approximately assuming it is homogeneous. Cannot be described. Furthermore, if the refractive index is different, the light speed is also different, and the analysis method based on the assumption that light propagates at a constant light speed cannot be established. Analyzes such as the microscopic Monte Carlo method cannot be solved unless the heterogeneous structure distribution is precisely assumed, and are not generally applicable analyses. Thus, the conventional method cannot perform accurate physical quantity analysis.
- the present invention enables accurate analysis of the physical quantity of a heterogeneous medium by separating the components of each constituent material and defining the relational expression with the light intensity for such a heterogeneous, multiphase, or mixed medium. It is intended to provide an optical analysis method for heterogeneous media.
- the intensity of light emitted from the inhomogeneous medium is detected, the detected light intensity is substituted for a predetermined function, and based on this function, the
- the solution of a system of differential equations that describes the situation in which light that meets light scattering between multiple tissues enters and exits is represented by a descriptive function.
- This function is dependent on the physical quantity of the inhomogeneous medium, and is a function that defines the relationship between the light intensity and the linear sum of exponential functions of the depth of light penetration with the base e. In this case, the physical quantity of the heterogeneous medium can be accurately analyzed.
- the physical quantity is defined by the absorption coefficient of the heterogeneous medium, the scattering coefficient of the heterogeneous medium, or the concentration of a predetermined component in the heterogeneous medium.
- I (e- at + e Z 2, where I is the intensity, t is the penetration depth, and unknown is the physical quantity dependent unknown.
- the light emitted by the reflection is the reflected light reflected by the inhomogeneous medium, and the amount (intensity) of the reflected light is represented by the linear sum of ( ⁇ +?) / ⁇ and 1 / ⁇ . It is a basic feature to be shown.
- Figure 1A shows the transmitted light intensity I obtained when the sample to be measured SM is irradiated with light LT.
- Figure 1B is a diagram for explaining the reflected light intensity u (e1), u (person 2), ..., u (person n) obtained when the sample SM to be measured is irradiated with light LT. is there.
- FIG. 2 is a diagram illustrating a method for analyzing a heterogeneous medium according to the embodiment.
- Fig. 1A is a basic example of transmission, and the continuous spectral intensities I (E1), I (People 2), ⁇ , I ( ⁇ ).
- Fig. 1 ⁇ is a basic example of the case of reflected light.
- the reflection obtained when the sample S ⁇ is irradiated with light L ⁇ It shows the continuous spectral intensity of light u (input 1), u (person 2), ⁇ , u ( ⁇ ).
- FIG. 2 is a diagram illustrating a method for analyzing a heterogeneous medium according to the embodiment.
- the continuous spectral data I input 1
- I person 2)
- ..., I ⁇
- u ⁇ 1
- u 2 u
- ⁇ u
- the above is a physical quantity measurement algorithm based on the assumption that the medium is homogeneous. Measurement and analysis of physical quantities in a heterogeneous medium requires a more accurate one.
- measurement and analysis of a physical quantity in a heterogeneous medium will be described.
- the case where the medium is heterogeneous is considered.
- the medium is a living tissue
- X tissue indicates a set of capillaries
- Y tissue indicates tissues other than blood vessels (such as fat and muscle tissue), and these are mixed.
- x, y is the volume defined by the product of the total cross-sectional area of the relevant tissue X, Y and the unit depth when considering the total cross-sectional area of the luminous flux probe being measured. It corresponds to the number of photons present in it. Or you can think of it as the photon density multiplied by the cross-sectional area of X, Y, and so on. Since the individual values of X and y are deleted later, there is no need to specify the density or cross section here.
- ⁇ a , ⁇ ax , ⁇ sx , i ay , sy,, E ⁇ P x , P y indicate the following.
- P y the concentration of the target substance in the Y tissue
- the light loss coefficient to other tissues is given by the product of the lateral light scattering coefficient // s ”and the structural coefficient E, s ” XE.
- the structural coefficient E defines the situation where light flows from the X organization to the Y organization and from the Y organization to the X organization.
- the mean free path of the photon is related to the sum of the absorption coefficient and the scattering coefficient. That is,
- Equation 5 the coefficients a, b, c, and d are given by the following equation (Equation 5). It should be noted that b and c are added independently of a and d.
- the suffix xy is related to each organization of X and Y It represents that.
- the cross-sectional area of each actual tissue is often sufficiently smaller than the cross-sectional area of the probe, and the light quantity I actually measured is always the sum of the light quantity X and the light quantity y.
- the measured light intensity I includes the effects of the X and Y tissues, and there is an interaction between the tissues. Therefore, assuming that the scattering is strong, the light amount is averaged between the two tissues.
- Equation 10 A useful expression that does not include the light penetration depth t can also be used as a theorem. Assuming that components of the coefficients a and d that do not escape to other organizations are m and n, m and n are given by the following (Equation 11).
- Equation 13 When the light quantity I is measured, the unknowns are two, and if the number of equations is two including (Equation 9), then Can be specified. This additional equation can be obtained, for example, by knowing the penetration depth t of light.
- the physical quantity of the target heterogeneous medium can be identified.
- all the information on heterogeneous media has been obtained from the above equation.
- the physical quantities include absorption coefficients, scattering coefficients, structure coefficients, and the concentration of the target substance (, ⁇ sx , ⁇ ay , ⁇ sy , E x , E y , P x , P y ) are listed. If the number of equations obtained from the experiment is equal to or larger than the number of unknowns, various physical quantities can be specified.
- the feature of this analysis method is that the properties such as the structure and interaction of the heterogeneous system are eliminated without being explicitly displayed, so that the solution can be obtained regardless of the values of b and c. Only Does not contain mathematical approximations, and can hold an exact solution representation. Therefore, a solution can be derived regardless of the inhomogeneous state without considering it. In other words, the present method can be applied to the case where the degree or cross-sectional area of each tissue fluctuates in the light traveling direction. The reason for this is that the structure and features (b, c) of the heterogeneous system are eventually eliminated without any constraints.
- each tissue-specific // a , ⁇ is the standard value of the absorption or scattering coefficient per unit molar concentration. Then, unknowns at each wavelength, for example, P x and P y can be determined.
- the measurement requires a minimum of three wavelengths, and preferably uses more wavelengths to reduce errors.
- the subsequent steps may be the same as described above.
- P x and P y to be obtained in the next stage may be directly used instead of hi and as unknowns.
- K-M Kube lka-Munk
- the reflected light that is, the intensity (u) of the return light
- Equation 14 the intensity of the return light
- Equation 14 L originally means the mean free path, but in the K-M method, it can be assumed that it corresponds to the normalized theoretical solution when returning in the opposite direction.
- S K is the reverse (backward) scattering coefficient may be displayed with ⁇ SK two ⁇ s g. In the case of the present invention, this is an averaged number for tissue X, Y. If this value is known, treat it as a known coefficient. If this value is unknown, it can be treated as an undetermined coefficient in the optimization numerical calculation described later.
- the reflected light is measured with light of at least three wavelengths, preferably with more wavelengths to reduce errors if possible.
- unknowns and Shitehi instead of 3, may be used [rho chi and P y to be obtained in the next step directly.
- the interaction term since the interaction term is included in a nonlinear display, it cannot be solved analytically until the end.
- b and c are constants independent of wavelength, and the spectra of all wavelengths are measured. Numerical meter considering the mixing ratio as an undetermined coefficient It can be solved by arithmetic.
- Equation 16 If the unknowns are P i and Pj and the undetermined coefficients are b and c, a solution can be obtained using at least four types of wavelength measurement values.
- Equation 20 This right side is approximated by a straight line excluding the origin, with s as the horizontal axis. This clearly shows that the method of the present invention is different from a simple exponential function in the homogeneous phase.
- u can be measured at a plurality of wavelengths, and these equations can be combined to solve.
- the expression of the solution is long, it is not described here.
- the method described has been applied to a steady state over time.
- the present patent is not limited to this, and can be applied to a case that changes with time.
- the time for light to travel from the input end to the output end itself is longer than the simple travel time due to the influence of multiple scattering. It is less than about a second. Therefore, particularly high-speed analysis corresponds to analysis of high-speed combustion reaction, explosion or destruction phenomenon. If the inside of the object is substantially at a low speed and the light source itself emits modulated light, all cases are applicable.
- Equation 21 The second term in the katakana on the right-hand side is a case where the absorption-scattering coefficient or the concentration changes.
- the b and c terms are almost constant, as mentioned above.
- Equation 23 In general, it can be treated as a perturbation. In other words, if a solution of the criterion equation is obtained, it can be used as an initial value and repeated calculations can be performed to optimize the (3a / 3) SX 5 dr, and the desired reaction parameter SaZS r is determined. I do.
- wavelength spectrum data may be used as needed to increase the calculation accuracy.
- the target solution can be reached without explicitly treating them. It is extremely effective for practical use.
- the heart pulsation status can be extracted by this optical property measurement, so that information on blood circulation can be obtained.
- the flow can be monitored.
- blood oxygen concentration is represented by the concentration of oxidized and reduced hemoglobin.
- it can be applied to quantitative measurement of blood glucose, red blood cells, cholesterol, neutral fat, etc. in blood, and can replace conventional blood tests by blood sampling. Absorption spectra of these components are well known and range from the visible to the infrared.
- the absorption coefficient and the scattering coefficient work in the medium in a linear additive manner.
- the X tissue is the intravascular blood portion
- the Y tissue is the other tissue portion
- x and y are the light amounts contained therein.
- the measurement light is emitted from the light source through the optical fiber and the wavelength filter to the inside of the living body.
- a large number of receiving fibers are placed around the outgoing fiber, and the reflected output is measured collectively. This is so that a wide area of, for example, about 1 cm 2 can be received so that the sum of the outputs from the X and y tissues can be measured.
- the signal-to-noise ratio can also be improved by intermittently intermitting the light at regular intervals as necessary and synchronously detecting the light receiving side.
- the calculation can be simplified by measuring the oxygen saturation etc. by another method only in the first case and using it as a comparison standard thereafter. However, it is not required.
- the portion to which the measurement probe is applied is the same for the measurement of required plural wavelengths. Since the above interaction terms are guaranteed to be common, the calculation accuracy and the like are improved. But it is not required. This is because the interaction term can be eliminated for each wavelength.
- the output light quantity is measured for each wavelength, and the result is made into a data list as a normalized number and stored in the storage device.
- Wavelength measurement is performed at multiple wavelengths, but for accurate measurement, the wavelength range is, for example, 450 to 150 nm, that is, it is accurate to perform measurement in the visible and near-infrared regions. Is preferred.
- the absorption spectrum of the wavelength range is acquired, and from the plurality of measured values, To determine the concentrations P x and P y at each wavelength using the above method, assign the measurement spectrum by the least square method based on oxidized hemoglobin, hemoglobin, cytochrome, and other known absorption spectra. Obtain the concentrations P x and P y .
- the concentration can be determined because the standard value of the absorption coefficient to be determined is known, but in general, the value at a certain wavelength is Since it is highly likely that the sum of the absorptions of a plurality of components is obtained, it is necessary to separate them. As described above, each component can be separated from the spectrum profile of the measured data, and then, at each wavelength, each beak value is compared with the expected absorption coefficient.
- the calculation proceeds as follows.
- the spectra of these light absorbers can be predicted in advance. For example, in the case of the human body, there are reduced and oxidized tissues such as cytochrome and hemoglobin. Since the light absorption coefficient and scattering coefficient for these single phases are known, they are stored in a storage device as a database, and this is used as a reference data to determine the matrix elements of the linear programming method. , Used for subsequent calculations.
- Equation 24 Equation 24
- z is the vector of the measured light amount for each wavelength
- ⁇ is the vector of the unknown concentration to be obtained. It is a kutor.
- each element of the Jacobi matrix is the differential coefficient between the vectors, but Since it can handle, the differential element of each matrix element can also be displayed analytically, and calculation can be performed. This is a major feature of the method.
- the absorption coefficient is obtained for each tissue at each wavelength, since the individual absorption spectrum is known, it is used as standard waveform data to match each component concentration measurement value. Apply linear programming to search for the ratio.
- a well-known numerical calculation program may be installed in a computer to perform the calculation in order to optimize the solution by solving the inverse matrix. In the inverse matrix calculation at this time, since the number of rows and the number of columns generally do not match as described above, it is necessary to combine the transposed matrix.
- the main composition is a single phase, it depends on the medium. Since it is generally known, it is easy to determine and subtract the background.
- component analysis for each component is performed by the multivariable nonlinear optimization method, and then the optimal solution can be approximately converged to obtain unknowns for the target component. At this time, the convergence at a high speed can be achieved by starting from a value of t which is almost expected as a starting value.
- the transmission thickness t is a position distant from the light incident point of the object to be measured.However, when measuring by emitting light from the same plane as the light incident surface, the principal ray path is not linear but significantly bent And a typical t value cannot be determined. In such a case, the method of assuming an average t-value in advance is that the attenuation is exponential to the path length, and the ray path is complicated. The route determination itself is also uncertain and should not be used because it introduces significant errors.
- the most accurate value can be determined. Furthermore, as described in the previous section, taking advantage of the fact that the measured values can be decomposed from the spectrum into the expected components, P x and P y are adjusted to match the most probable component values. Can be determined.
- the theory has an analytical expression.
- the interaction terms b and c are eliminated by combining the expression of Z and the above theorems, and (hi, ⁇ ) or (a, d) is solved. Since these are the linear sums of the absorption coefficients of the constituents in each tissue, they can be solved by linear programming. Also in this case, the accuracy is higher when the least square method is applied to all the measured wavelengths.
- the method of the present invention considers that the light quantity can be described by a linear combination of two exponential functions with power exponent and a function derived from those exponents. There is essence in using. Through this expression, it is possible to express the optical constant of a medium that exhibits inhomogeneous and strong scattering, and a structure related to inhomogeneity. There is an advantage that uncertain factors such as construction need not be dealt with explicitly.
- Objects in living organisms, plants, leaves, fruits, wood, powders, solid-liquid mixtures, and emulsions, sewage, seawater, and fumes have strong scattering characteristics as well as non-uniformity, and are important in practical use. Note that many errors occur in these optical measurements. Since these heterogeneous internal structures show various and complicated situations, it is almost impossible to measure these situations while specifying them.
- the method of the present invention proposes a mathematical method to reach the result without explicitly treating these complicated elements.
- the method is based on completely mathematically rigorous principles and is suitable for dealing with complex problems. Therefore, the method of the present invention is suitable for the measurement of a material with inhomogeneous and strong scattering as exemplified above.
- the method described above utilizes the fact that the amount of light is the sum of exponential functions due to the non-uniform structure.
- the measurement result was corrected for the homogeneous phase
- a correct value can be calculated from the above combination equation.
- the intensity of light emitted from the heterogeneous medium is detected, and the intensity of the detected light depends on the physical quantity of the heterogeneous medium.
- the physical quantity of the heterogeneous medium is determined based on a function represented by a linear sum of exponential functions of the penetration depth with base e and a relational expression derived from this function. In this case, the optical analysis of the heterogeneous medium can be accurately performed.
- this method when the heterogeneous medium is heterogeneous or has a multiphase or multiphase-separated structure such as a mixture, this method is applied to separate each phase component in each tissue and calculate independently. be able to.
- the system is reduced from the simultaneous equations including the number of mediating elements according to the number of tissue types, and this is applied to the above analysis method when there are two tissues X and Y. be able to.
- the method according to the above-described embodiment is an optical analysis method for determining a physical quantity of a medium from the intensity of light transmitted or reflected through the medium, and particularly has optical characteristics different from those of the medium.
- Light intensity A function defined as the solution of a system of differential equations describing distribution, which is dependent on the physical quantity of the inhomogeneous medium and is described by the linear sum of the exponential function of the depth of penetration of light with base e and the function derived from this It is characterized by that. According to this method, The physical quantity of the medium can be accurately analyzed.
- the exponential function when the emitted light is transmitted light from the heterogeneous medium, the exponential function is: the intensity is I, the penetration depth is t, and the exponent of the exponential function is ⁇ .
- the emitted light is reflected light reflected by the inhomogeneous medium, and when a function related to the amount of reflected light is composed of two tissues, (h + ⁇ ) / h and It is preferably a linear sum of 1 Z.
- the physical quantity is preferably an absorption coefficient of the heterogeneous medium, a scattering coefficient of the heterogeneous medium, or a concentration of a predetermined component in the heterogeneous medium.
- the method includes making use of the fact that the mutual scattering coefficient defining the light quantity redistribution is equal in many cases, and using a method that facilitates the calculation.
- This invention can be utilized for the optical analysis method of a heterogeneous medium.
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU38422/00A AU3842200A (en) | 1999-04-21 | 2000-04-21 | Method for optically analyzing inhomogeneous medium |
EP00917419A EP1182444B1 (en) | 1999-04-21 | 2000-04-21 | Method for optically analyzing an inhomogeneous medium |
DE60035983T DE60035983T2 (de) | 1999-04-21 | 2000-04-21 | Verfahren zur optischen analyse eines inhomogenen mediums |
US09/982,022 US6643020B2 (en) | 1999-04-21 | 2001-10-19 | Optical analysis method for inhomogeneous turbid media |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP11370899 | 1999-04-21 | ||
JP11/113708 | 1999-04-21 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09/982,022 Continuation-In-Part US6643020B2 (en) | 1999-04-21 | 2001-10-19 | Optical analysis method for inhomogeneous turbid media |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2000065330A1 true WO2000065330A1 (fr) | 2000-11-02 |
Family
ID=14619160
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2000/002638 WO2000065330A1 (fr) | 1999-04-21 | 2000-04-21 | Procede d'analyse optique de milieu non homogene |
Country Status (5)
Country | Link |
---|---|
US (1) | US6643020B2 (ja) |
EP (1) | EP1182444B1 (ja) |
AU (1) | AU3842200A (ja) |
DE (1) | DE60035983T2 (ja) |
WO (1) | WO2000065330A1 (ja) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003056307A1 (fr) * | 2001-12-26 | 2003-07-10 | Hamamatsu Photonics K.K. | Technique d'analyse optique pour milieu heterogene |
JP2003202287A (ja) * | 2002-01-08 | 2003-07-18 | Hamamatsu Photonics Kk | 散乱吸収体測定方法及び装置 |
JP2008157832A (ja) * | 2006-12-26 | 2008-07-10 | National Institute Of Advanced Industrial & Technology | 生体光計測装置 |
JP2010266377A (ja) * | 2009-05-15 | 2010-11-25 | Yoshihiko Mizushima | 不均一物質の光分析方法 |
WO2014087825A1 (ja) * | 2012-12-06 | 2014-06-12 | 国立大学法人北海道大学 | 非侵襲型生体脂質濃度計測器、非侵襲型生体脂質代謝機能計測器、非侵襲による生体脂質濃度計測方法および非侵襲による生体脂質代謝機能検査方法 |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007119202A1 (en) * | 2006-04-18 | 2007-10-25 | Koninklijke Philips Electronics N.V. | Optical measurement device |
JP4858617B2 (ja) * | 2007-11-29 | 2012-01-18 | 株式会社島津製作所 | 断層撮影装置 |
CN101292875B (zh) * | 2008-06-06 | 2010-07-14 | 天津市先石光学技术有限公司 | 利用基准波长测量成分浓度的方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH06343625A (ja) * | 1993-06-10 | 1994-12-20 | Hamamatsu Photonics Kk | 散乱媒質内吸光物質の濃度測定方法および装置 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4972331A (en) | 1989-02-06 | 1990-11-20 | Nim, Inc. | Phase modulated spectrophotometry |
JP2539707B2 (ja) | 1991-03-27 | 1996-10-02 | 大塚電子株式会社 | 吸光スペクトルの補正方法およびその方法を用いた光拡散物質の分光測定装置 |
JP3577335B2 (ja) * | 1993-06-02 | 2004-10-13 | 浜松ホトニクス株式会社 | 散乱吸収体計測方法及び装置 |
JP3844815B2 (ja) * | 1996-08-30 | 2006-11-15 | 浜松ホトニクス株式会社 | 散乱体の吸収情報計測方法及び装置 |
JP3887486B2 (ja) * | 1998-05-26 | 2007-02-28 | 浜松ホトニクス株式会社 | 散乱吸収体の内部特性分布の計測方法及び装置 |
DE19831424C2 (de) | 1998-07-14 | 2000-12-28 | Mbr Gmbh | Spektroskopisches Verfahren zur Bestimmung der Konzentration eines in einem streuenden Medium verteilten Stoffes |
-
2000
- 2000-04-21 AU AU38422/00A patent/AU3842200A/en not_active Abandoned
- 2000-04-21 WO PCT/JP2000/002638 patent/WO2000065330A1/ja active IP Right Grant
- 2000-04-21 DE DE60035983T patent/DE60035983T2/de not_active Expired - Fee Related
- 2000-04-21 EP EP00917419A patent/EP1182444B1/en not_active Expired - Lifetime
-
2001
- 2001-10-19 US US09/982,022 patent/US6643020B2/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH06343625A (ja) * | 1993-06-10 | 1994-12-20 | Hamamatsu Photonics Kk | 散乱媒質内吸光物質の濃度測定方法および装置 |
Non-Patent Citations (2)
Title |
---|
See also references of EP1182444A4 * |
UEDA Y. ET AL.: "Progress in biomedical optics", THE INTERNATIONAL BIOMEDICAL OPTICS SOCIETY, vol. 2979, 1997, pages 795 - 806, XP002930761 * |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003056307A1 (fr) * | 2001-12-26 | 2003-07-10 | Hamamatsu Photonics K.K. | Technique d'analyse optique pour milieu heterogene |
EP1460412A1 (en) * | 2001-12-26 | 2004-09-22 | Hamamatsu Photonics K. K. | Optical analysis method for heterogeneous medium |
EP1460412A4 (en) * | 2001-12-26 | 2009-12-02 | Hamamatsu Photonics Kk | OPTICAL ANALYSIS METHOD FOR HETEROGENE MEDIUM |
JP2003202287A (ja) * | 2002-01-08 | 2003-07-18 | Hamamatsu Photonics Kk | 散乱吸収体測定方法及び装置 |
JP2008157832A (ja) * | 2006-12-26 | 2008-07-10 | National Institute Of Advanced Industrial & Technology | 生体光計測装置 |
JP2010266377A (ja) * | 2009-05-15 | 2010-11-25 | Yoshihiko Mizushima | 不均一物質の光分析方法 |
WO2014087825A1 (ja) * | 2012-12-06 | 2014-06-12 | 国立大学法人北海道大学 | 非侵襲型生体脂質濃度計測器、非侵襲型生体脂質代謝機能計測器、非侵襲による生体脂質濃度計測方法および非侵襲による生体脂質代謝機能検査方法 |
JPWO2014087825A1 (ja) * | 2012-12-06 | 2017-01-05 | 国立大学法人北海道大学 | 非侵襲型生体脂質濃度計測器、非侵襲型生体脂質代謝機能計測器、非侵襲による生体脂質濃度計測方法および非侵襲による生体脂質代謝機能検査方法 |
US10188328B2 (en) | 2012-12-06 | 2019-01-29 | National University Corporation Hokkaido University | Non-invasive biolipid concentration meter, non-invasive biolipid metabolism measuring device, non-invasive method for measuring biolipid concentration, and non-invasive method for examining biolipid metabolism |
US11331012B2 (en) | 2012-12-06 | 2022-05-17 | Medical Photonics Co., Ltd. | Non-invasive biolipid concentration meter, non-invasive biolipid metabolism measuring device, non-invasive method for measuring biolipid concentration, and non-invasive method for examining biolipid metabolism |
Also Published As
Publication number | Publication date |
---|---|
EP1182444A4 (en) | 2004-06-23 |
US6643020B2 (en) | 2003-11-04 |
AU3842200A (en) | 2000-11-10 |
EP1182444B1 (en) | 2007-08-15 |
EP1182444A1 (en) | 2002-02-27 |
DE60035983T2 (de) | 2008-05-15 |
DE60035983D1 (de) | 2007-09-27 |
US20020082504A1 (en) | 2002-06-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11274967B2 (en) | Method and apparatus for quantifying solutions comprised of multiple analytes | |
Matousek et al. | Subsurface probing in diffusely scattering media using spatially offset Raman spectroscopy | |
CN104958075B (zh) | 使用拉曼光谱非侵入性测量皮肤厚度和血糖浓度及其校准方法 | |
CN105510238B (zh) | 多位置漫射光谱数据的处理、建模、预测方法和处理装置 | |
Xu et al. | A calibration method free of optimum factor number selection for automated multivariate analysis. Experimental and theoretical study | |
Zhang et al. | Discussion on the validity of NIR spectral data in non-invasive blood glucose sensing | |
Shih et al. | Intrinsic Raman spectroscopy for quantitative biological spectroscopy part I: theory and simulations | |
Cécillon et al. | Spectral fingerprinting of soil organic matter composition | |
Bricklemyer et al. | Improved intact soil-core carbon determination applying regression shrinkage and variable selection techniques to complete spectrum laser-induced breakdown spectroscopy (LIBS) | |
WO2000065330A1 (fr) | Procede d'analyse optique de milieu non homogene | |
Vogt et al. | A UV spectroscopic method for monitoring aromatic hydrocarbons dissolved in water | |
Tian et al. | WSPXY combined with BP-ANN method for hemoglobin determination based on near-infrared spectroscopy | |
Yang et al. | Space-enhanced time-domain diffuse optics for determination of tissue optical properties in two-layered structures | |
Cano-Trujillo et al. | Differentiation of blood and environmental interfering stains on substrates by Chemometrics-Assisted ATR FTIR spectroscopy | |
Mauricio-Iglesias et al. | Application of FTIR and Raman microspectroscopy to the study of food/packaging interactions | |
Li et al. | Reagent-free simultaneous determination of glucose and cholesterol in whole blood by FTIR-ATR | |
US9823185B1 (en) | NDIR reflection sampling in liquids | |
Lübbers et al. | Absolute reflection photometry at organ surfaces | |
US9726601B1 (en) | NDIR interference control in liquids | |
JP4084307B2 (ja) | 不均質媒質の光学的分析方法 | |
Pereira et al. | A flow system for generation of concentration perturbation in two-dimensional correlation near-infrared spectroscopy: application to variable selection in multivariate calibration | |
Yao et al. | Wavelength selection method based on absorbance value optimization to near-infrared spectroscopic analysis | |
Lin et al. | An improved system for noninvasive detection of lymphocytes by dynamic spectroscopy | |
Luo et al. | Reducing collinearity by reforming spectral lines with two-dimensional variable selection method | |
Li et al. | A combined multi-pathlength and wavelength optimization method for accurate detection of platelet count |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
ENP | Entry into the national phase |
Ref country code: JP Ref document number: 2000 614019 Kind code of ref document: A Format of ref document f/p: F |
|
WWE | Wipo information: entry into national phase |
Ref document number: 09982022 Country of ref document: US |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2000917419 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWP | Wipo information: published in national office |
Ref document number: 2000917419 Country of ref document: EP |
|
WWG | Wipo information: grant in national office |
Ref document number: 2000917419 Country of ref document: EP |