WO2000031538A1 - Essais en ecoulement lateral ameliores - Google Patents

Essais en ecoulement lateral ameliores Download PDF

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Publication number
WO2000031538A1
WO2000031538A1 PCT/US1999/027497 US9927497W WO0031538A1 WO 2000031538 A1 WO2000031538 A1 WO 2000031538A1 US 9927497 W US9927497 W US 9927497W WO 0031538 A1 WO0031538 A1 WO 0031538A1
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WO
WIPO (PCT)
Prior art keywords
analyte
control
agent
test strip
binding
Prior art date
Application number
PCT/US1999/027497
Other languages
English (en)
Inventor
Alan J. Polito
Richard M. Thayer
Robert K. Dinello
George H. Sierra
Original Assignee
Praxsys Biosystems, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Praxsys Biosystems, Inc. filed Critical Praxsys Biosystems, Inc.
Priority to AU17390/00A priority Critical patent/AU1739000A/en
Publication of WO2000031538A1 publication Critical patent/WO2000031538A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nanotechnology (AREA)
  • Inorganic Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

La présente invention concerne des essais en écoulement latéral (papier réactif) caractérisés par: une ou plusieurs zones de contrôle possédant un agent de contrôle (dinitrophénol par exemple) immobilisé dans ces dernières; un agent de détection consistant en une conjugaison de liant d'analyte (extrait d'helicobacter pylori ou antigène d'enveloppe HIV par exemple), de liant de contrôle (anticorps dirigé contre le dinitrophénol) et d'un marqueur (or colloïdal 16 nm, permettant les mesures de réflectance).
PCT/US1999/027497 1998-11-23 1999-11-18 Essais en ecoulement lateral ameliores WO2000031538A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU17390/00A AU1739000A (en) 1998-11-23 1999-11-18 Improved lateral flow assays

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US19811898A 1998-11-23 1998-11-23
US09/198,118 1998-11-23
US09/442,383 1999-11-17

Publications (1)

Publication Number Publication Date
WO2000031538A1 true WO2000031538A1 (fr) 2000-06-02

Family

ID=22732055

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/027497 WO2000031538A1 (fr) 1998-11-23 1999-11-18 Essais en ecoulement lateral ameliores

Country Status (2)

Country Link
CN (1) CN1254844A (fr)
WO (1) WO2000031538A1 (fr)

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1143248A2 (fr) * 2000-02-29 2001-10-10 Boehringer Mannheim Gmbh Méthode pour l'immobilisation de conjugates dans des épreuves diagnostiques
WO2003048771A2 (fr) * 2001-12-04 2003-06-12 Lattec I/S Dispositif permettant d'analyser des analytes et utilisation correspondante
EP1416275A1 (fr) * 2002-10-31 2004-05-06 Oy Reagena Ltd Procédé de test rapide et dispositiv de contrôle
EP1507148A1 (fr) * 2003-08-25 2005-02-16 MTM Laboratories AG Procédé de détection de carcinomes dans des échantillons corporels cervicaux solubilisés
WO2005111607A2 (fr) 2004-05-12 2005-11-24 Roche Diagnostics Gmbh Procede pour elargir la plage de mesure dynamique d'elements servant a realiser des tests notamment immunologiques, bases sur des reactions de fixation specifiques
WO2006003394A1 (fr) * 2004-07-01 2006-01-12 Central Science Laboratory (Csl) Representing The Secretary Of State For Environment, Food And Rural Affairs Systeme de detection d'analytes
WO2006036247A1 (fr) * 2004-09-28 2006-04-06 Kimberly-Clark Worldwide, Inc. Detection d'infections a levures par dosage a ecoulement lateral
EP1808696A1 (fr) * 2006-01-14 2007-07-18 Roche Diagnostics GmbH Elément de test immunologique doté d'une zone de contrôle améliorée
US20090180928A1 (en) * 2005-04-22 2009-07-16 Alverix, Inc. Assay test strips with multiple labels and reading same
GB2475630A (en) * 2006-03-11 2011-05-25 Central Science Lab Representing The Sec Dep For Environment Food And Rural Affairs Two-stage detection assay
US8043811B2 (en) 2006-03-11 2011-10-25 The Food & Environment Research Agency (FERA) representing the Secretary of State for Environment, Food and Rural Affairs Purification method and kits
WO2013040435A3 (fr) * 2011-09-16 2013-05-23 Credo Biomedical Pte Ltd Dispositif de dosage de diagnostic moléculaire et procédé d'utilisation
EP2271939B1 (fr) * 2008-04-09 2015-06-17 Becton Dickinson and Company Immuno-essais sensibles utilisant des nanoparticules revêtues
CN106841602A (zh) * 2017-01-21 2017-06-13 上海源紊新能源科技有限公司 一种邻苯二甲酸二丙烯酯胶体金免疫层析检测试纸
CN107907679A (zh) * 2017-11-06 2018-04-13 南京诺唯赞医疗科技有限公司 一种免疫层析试纸条及其制作方法与应用
US9989527B2 (en) 2004-04-01 2018-06-05 Alverix, Inc. Lateral flow assay systems and methods
JP6426872B1 (ja) * 2018-08-02 2018-11-21 積水メディカル株式会社 イムノクロマト用試験片及びイムノクロマト検出キット
CN111157721A (zh) * 2020-01-15 2020-05-15 珠海丽珠试剂股份有限公司 用于提高肺炎衣原体抗原/支原体抗原在免疫层析试剂中稳定性的包被液及其制备方法
CN111413501A (zh) * 2018-01-30 2020-07-14 深圳市伯劳特生物制品有限公司 一种检测试纸条在制备检测thsd7a抗体的试剂盒中的应用
CN111413506A (zh) * 2018-01-30 2020-07-14 深圳市伯劳特生物制品有限公司 一种检测试纸条在制备检测pla2r抗体的试剂盒中的应用

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008105814A2 (fr) 2006-08-22 2008-09-04 Los Alamos National Security, Llc Dispositif à écoulement latéral miniaturisé pour détection rapide et sensible de protéines ou d'acides nucléiques
US8980561B1 (en) 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
JP2011522521A (ja) 2008-05-05 2011-08-04 ロスアラモス ナショナル セキュリティ，エルエルシー 高度に単純化された側方流動ベースの核酸サンプル調製および受動的流体流動制御
KR101027036B1 (ko) * 2009-05-28 2011-04-11 주식회사 인포피아 금 이온의 환원에 의한 측방유동 분석에서의 신호 증폭 방법 및 이를 이용한 측방유동 분석 디바이스
CA2856304C (fr) 2011-04-20 2017-05-16 Mesa Tech International, Inc. Reaction d'amplification d'oscillation pour des acides nucleiques
CN102879571A (zh) * 2012-09-25 2013-01-16 中华人民共和国淮安出入境检验检疫局 一种快速检测铜离子的胶体金增敏层析试纸条
CN104251908B (zh) * 2013-06-28 2016-03-30 广州万孚生物技术股份有限公司 样品垫处理液、h7亚型禽流感病毒胶体金试纸条及其制备方法
CN105044366A (zh) * 2015-07-15 2015-11-11 宁波高新区绿邦科技发展有限公司 新型盐酸克伦特罗检测试剂卡
CN105092567B (zh) * 2015-07-15 2019-01-29 宁波高新区绿邦科技发展有限公司 盐酸克伦特罗-莱克多巴胺-沙丁胺醇三联检测试剂卡
CN105203723A (zh) * 2015-07-15 2015-12-30 宁波高新区绿邦科技发展有限公司 快速定量检测盐酸克伦特罗的检测装置及其检测方法
CN107037211B (zh) * 2016-11-04 2019-03-01 苏州海苗生物科技有限公司 一种定量检测化合物的测试条及侧向免疫层析的检测方法
CN112946253B (zh) * 2019-12-11 2022-11-08 广东菲鹏生物有限公司 用于免疫诊断试剂中固相载体的封闭阶段处理剂及应用和产品
CN112362864B (zh) * 2021-01-12 2021-04-30 广州科方生物技术股份有限公司 一种降低免疫诊断试剂背景发光值的处理剂及其制备方法
CN116930513B (zh) * 2023-09-19 2023-11-17 北京索莱宝科技有限公司 一种用于蛋白检测的无蛋白快速封闭液及其应用

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US5661019A (en) * 1987-09-30 1997-08-26 Beckman Instruments, Inc. Trifunctional conjugates
EP0833157A1 (fr) * 1996-09-27 1998-04-01 Unilever Plc Réactifs et dispositifs d'analyse

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US5661019A (en) * 1987-09-30 1997-08-26 Beckman Instruments, Inc. Trifunctional conjugates
WO1997009620A1 (fr) * 1995-09-07 1997-03-13 Agen Biomedical Limited Procede et appareil destines a l'evaluation quantitative et semi-quantitative d'une substance a analyser
EP0833157A1 (fr) * 1996-09-27 1998-04-01 Unilever Plc Réactifs et dispositifs d'analyse

Cited By (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7241418B2 (en) 2000-02-29 2007-07-10 Roche Diagnostics Corporation Method for immobilizing conjugates in diagnostic tests
EP1143248A3 (fr) * 2000-02-29 2004-02-11 Boehringer Mannheim Gmbh Méthode pour l'immobilisation de conjugates dans des épreuves diagnostiques
EP1143248A2 (fr) * 2000-02-29 2001-10-10 Boehringer Mannheim Gmbh Méthode pour l'immobilisation de conjugates dans des épreuves diagnostiques
US6998246B2 (en) 2000-02-29 2006-02-14 Roche Diagnostics Corporation Method for immobilizing conjugates in diagnostic tests
US7241628B2 (en) 2000-02-29 2007-07-10 Roche Diagnostics Corporation Method for immobilizing conjugates in diagnostic tests
WO2003048771A2 (fr) * 2001-12-04 2003-06-12 Lattec I/S Dispositif permettant d'analyser des analytes et utilisation correspondante
WO2003048771A3 (fr) * 2001-12-04 2003-11-27 Lattec I S Dispositif permettant d'analyser des analytes et utilisation correspondante
EP1416275A1 (fr) * 2002-10-31 2004-05-06 Oy Reagena Ltd Procédé de test rapide et dispositiv de contrôle
EP1507148A1 (fr) * 2003-08-25 2005-02-16 MTM Laboratories AG Procédé de détection de carcinomes dans des échantillons corporels cervicaux solubilisés
WO2005088311A1 (fr) * 2003-08-25 2005-09-22 Mtm Laboratories Ag Methode de detection des troubles neoplasiques a partir d'un echantillon corporel solubilise
US7932047B2 (en) 2003-08-25 2011-04-26 Mtm Laboratories, Ag Method for detecting neoplastic disorders in a solubilized body sample
US9989527B2 (en) 2004-04-01 2018-06-05 Alverix, Inc. Lateral flow assay systems and methods
US8354235B2 (en) 2004-05-12 2013-01-15 Roche Diagnostics Operations, Inc. Method for increasing the dynamic measuring range of test elements based on specific binding reactions
WO2005111607A2 (fr) 2004-05-12 2005-11-24 Roche Diagnostics Gmbh Procede pour elargir la plage de mesure dynamique d'elements servant a realiser des tests notamment immunologiques, bases sur des reactions de fixation specifiques
GB2433989A (en) * 2004-07-01 2007-07-11 Central Science Lab Analyte delection system
GB2433989B (en) * 2004-07-01 2009-04-08 Central Science Lab Analyte detection system
WO2006003394A1 (fr) * 2004-07-01 2006-01-12 Central Science Laboratory (Csl) Representing The Secretary Of State For Environment, Food And Rural Affairs Systeme de detection d'analytes
US7763433B2 (en) 2004-07-01 2010-07-27 Forsite Diagnostics Limited Analyte detection system
AU2005259012B2 (en) * 2004-07-01 2011-01-20 Forsite Diagnostics Limited Analyte detection system
WO2006036247A1 (fr) * 2004-09-28 2006-04-06 Kimberly-Clark Worldwide, Inc. Detection d'infections a levures par dosage a ecoulement lateral
US10753931B2 (en) 2005-04-22 2020-08-25 Alverix, Inc. Assay test strips with multiple labels and reading same
US10191043B2 (en) 2005-04-22 2019-01-29 Alverix, Inc. Methods and systems for calibrating illumination source of diagnostic test system
US20090180928A1 (en) * 2005-04-22 2009-07-16 Alverix, Inc. Assay test strips with multiple labels and reading same
US10041941B2 (en) * 2005-04-22 2018-08-07 Alverix, Inc. Assay test strips with multiple labels and reading same
US11782058B2 (en) 2005-04-22 2023-10-10 Alverix, Inc. Diagnostic test system using measurement obtained from reference feature to modify operational parameter of reader
US9891217B2 (en) 2005-04-22 2018-02-13 Alverix, Inc. Assay test strips with multiple labels and reading same
US9528986B2 (en) 2006-01-14 2016-12-27 Roche Diagnostics Operations, Inc. Immunological test element with control zone for identifying hook effect
US10429382B2 (en) 2006-01-14 2019-10-01 Roche Diagnostics Operations, Inc. Immunological test element with control zone for identifying hook effect
EP1808696A1 (fr) * 2006-01-14 2007-07-18 Roche Diagnostics GmbH Elément de test immunologique doté d'une zone de contrôle améliorée
US8951806B2 (en) 2006-01-14 2015-02-10 Roche Diagnostics Operations, Inc. Immunological test element with improved control zone
US8043811B2 (en) 2006-03-11 2011-10-25 The Food & Environment Research Agency (FERA) representing the Secretary of State for Environment, Food and Rural Affairs Purification method and kits
GB2475630A (en) * 2006-03-11 2011-05-25 Central Science Lab Representing The Sec Dep For Environment Food And Rural Affairs Two-stage detection assay
EP2271939B1 (fr) * 2008-04-09 2015-06-17 Becton Dickinson and Company Immuno-essais sensibles utilisant des nanoparticules revêtues
EP2952897A1 (fr) * 2008-04-09 2015-12-09 Becton, Dickinson and Company Immunoessais sensibles à l'aide de nanoparticules revêtues
WO2013040435A3 (fr) * 2011-09-16 2013-05-23 Credo Biomedical Pte Ltd Dispositif de dosage de diagnostic moléculaire et procédé d'utilisation
US10598656B2 (en) 2011-09-16 2020-03-24 Credo Biomedical Pte Ltd. Method of selecting analyte to samples using a lateral flow device
CN106841602A (zh) * 2017-01-21 2017-06-13 上海源紊新能源科技有限公司 一种邻苯二甲酸二丙烯酯胶体金免疫层析检测试纸
CN107907679A (zh) * 2017-11-06 2018-04-13 南京诺唯赞医疗科技有限公司 一种免疫层析试纸条及其制作方法与应用
CN111413506A (zh) * 2018-01-30 2020-07-14 深圳市伯劳特生物制品有限公司 一种检测试纸条在制备检测pla2r抗体的试剂盒中的应用
CN111413501A (zh) * 2018-01-30 2020-07-14 深圳市伯劳特生物制品有限公司 一种检测试纸条在制备检测thsd7a抗体的试剂盒中的应用
JP6426872B1 (ja) * 2018-08-02 2018-11-21 積水メディカル株式会社 イムノクロマト用試験片及びイムノクロマト検出キット
JP2020020724A (ja) * 2018-08-02 2020-02-06 積水メディカル株式会社 イムノクロマト用試験片及びイムノクロマト検出キット
CN111157721A (zh) * 2020-01-15 2020-05-15 珠海丽珠试剂股份有限公司 用于提高肺炎衣原体抗原/支原体抗原在免疫层析试剂中稳定性的包被液及其制备方法
CN111157721B (zh) * 2020-01-15 2022-12-09 珠海丽珠试剂股份有限公司 用于提高肺炎衣原体抗原/支原体抗原在免疫层析试剂中稳定性的包被液及其制备方法

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