WO2000027365A8 - Nanocristaux fonctionnalises et leur utilisation dans des systemes de detection - Google Patents

Nanocristaux fonctionnalises et leur utilisation dans des systemes de detection

Info

Publication number
WO2000027365A8
WO2000027365A8 PCT/US1999/026487 US9926487W WO0027365A8 WO 2000027365 A8 WO2000027365 A8 WO 2000027365A8 US 9926487 W US9926487 W US 9926487W WO 0027365 A8 WO0027365 A8 WO 0027365A8
Authority
WO
WIPO (PCT)
Prior art keywords
functionalized nanocrystals
substrate
nanocrystals
functionalized
complexes
Prior art date
Application number
PCT/US1999/026487
Other languages
English (en)
Other versions
WO2000027365A1 (fr
WO2000027365A9 (fr
Inventor
Emilio Barbera-Guillem
Stephanie Castro
Original Assignee
Biocrystal Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/372,729 external-priority patent/US6114038A/en
Application filed by Biocrystal Ltd filed Critical Biocrystal Ltd
Priority to EP99965776A priority Critical patent/EP1128818A4/fr
Priority to AU21470/00A priority patent/AU2147000A/en
Publication of WO2000027365A1 publication Critical patent/WO2000027365A1/fr
Publication of WO2000027365A8 publication Critical patent/WO2000027365A8/fr
Publication of WO2000027365A9 publication Critical patent/WO2000027365A9/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G01N23/22—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material
    • G01N23/223—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material by irradiating the sample with X-rays or gamma-rays and by measuring X-ray fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2223/00—Investigating materials by wave or particle radiation
    • G01N2223/07—Investigating materials by wave or particle radiation secondary emission
    • G01N2223/076—X-ray fluorescence

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nanotechnology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Materials Engineering (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne des compositions renfermant des nanocristaux hydrosolubles fonctionnalisés. Ces nanocristaux hydrosolubles fonctionnalisés comprennent des points quantiques coiffés d'une couche d'un composé de coiffage, ainsi qu'un ou plusieurs composés additionnels, liés par la suite de manière opérationnelle. Un composé additionnel contient de préférence de l'acide diaminocarboxylique lié de manière opérationnelle audit composé de coiffage, ainsi qu'un acide aminé, un ligand d'affinité, ou une combinaison de ceux-ci. Cette invention concerne par ailleurs des procédés d'utilisation des nanocristaux fonctionnalisés susmentionnés comprenant un ligand d'affinité, ces procédés étant destinés à détecter la présence ou l'absence d'un substrat cible dans un échantillon. Ces procédés consistent tout d'abord à mettre lesdits nanocristaux fonctionnalisés en présence de cet échantillon, afin de former des complexes entre les nanocristaux et le substrat, si ce dernier est présent, puis à exposer ces complexes du système de détection à une source de lumière d'excitation, et enfin à détecter le pic de fluorescence émise.
PCT/US1999/026487 1998-11-10 1999-11-10 Nanocristaux fonctionnalises et leur utilisation dans des systemes de detection WO2000027365A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP99965776A EP1128818A4 (fr) 1998-11-10 1999-11-10 Nanocristaux fonctionnalises et leur utilisation dans des systemes de detection
AU21470/00A AU2147000A (en) 1998-11-10 1999-11-10 Functionalized nanocrystals and their use in detection systems

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US10962698P 1998-11-24 1998-11-24
US60/109,626 1998-11-24
US09/372,729 1999-08-11
US09/372,729 US6114038A (en) 1998-11-10 1999-08-11 Functionalized nanocrystals and their use in detection systems

Publications (3)

Publication Number Publication Date
WO2000027365A1 WO2000027365A1 (fr) 2000-05-18
WO2000027365A8 true WO2000027365A8 (fr) 2000-09-14
WO2000027365A9 WO2000027365A9 (fr) 2000-11-23

Family

ID=26807171

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/026487 WO2000027365A1 (fr) 1998-11-10 1999-11-10 Nanocristaux fonctionnalises et leur utilisation dans des systemes de detection

Country Status (1)

Country Link
WO (1) WO2000027365A1 (fr)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6607829B1 (en) 1997-11-13 2003-08-19 Massachusetts Institute Of Technology Tellurium-containing nanocrystalline materials
US6207392B1 (en) 1997-11-25 2001-03-27 The Regents Of The University Of California Semiconductor nanocrystal probes for biological applications and process for making and using such probes
US6617583B1 (en) 1998-09-18 2003-09-09 Massachusetts Institute Of Technology Inventory control
US6251303B1 (en) 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
US6306610B1 (en) 1998-09-18 2001-10-23 Massachusetts Institute Of Technology Biological applications of quantum dots
US6261779B1 (en) 1998-11-10 2001-07-17 Bio-Pixels Ltd. Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system
JP2003531477A (ja) 2000-03-14 2003-10-21 マサチューセッツ インスティテュート オブ テクノロジー 光学増幅器およびレーザー
CA2403620C (fr) 2000-03-20 2012-01-10 Massachusetts Institute Of Technology Bioconjugues chromophore inorganique
WO2001090717A2 (fr) * 2000-05-24 2001-11-29 Vanderbilt University Bras de liaison pour nanocristaux et composes correspondants
EP1348034B1 (fr) * 2000-11-15 2016-07-20 Minerva Biotechnologies Corporation Identifieurs d'oligonucleotides
US20020110180A1 (en) 2001-02-09 2002-08-15 Barney Alfred A. Temperature-sensing composition
EP1366347B8 (fr) 2001-03-09 2010-02-03 Univ Reims Champagne Ardenne L Nanocristaux ultrasensibles, non isotopes, solubles dans l'eau
GB0113772D0 (en) * 2001-06-06 2001-07-25 Dynal Biotech Asa Process
ATE431567T1 (de) 2001-09-17 2009-05-15 Massachusetts Inst Technology Halbleiternanokristall-verbundstoff
US7205048B2 (en) * 2001-09-17 2007-04-17 Invitrogen Corporation Functionalized fluorescent nanocrystal compositions and methods of making
CA2466821A1 (fr) 2001-11-21 2003-06-05 Applera Corporation Dosage biologique numerique
US7998923B2 (en) 2002-05-07 2011-08-16 The Regents Of The University Of California Bioactivation of particles
US7319709B2 (en) 2002-07-23 2008-01-15 Massachusetts Institute Of Technology Creating photon atoms
US7160613B2 (en) 2002-08-15 2007-01-09 Massachusetts Institute Of Technology Stabilized semiconductor nanocrystals
US7181266B2 (en) 2003-03-04 2007-02-20 Massachusetts Institute Of Technology Materials and methods for near-infrared and infrared lymph node mapping
US7917298B1 (en) 2003-04-17 2011-03-29 Nanosys, Inc. Nanocrystal taggants
EP1664777A1 (fr) * 2003-09-09 2006-06-07 Koninklijke Philips Electronics N.V. Nanoparticules destinees a la detection d'analytes
US7943396B2 (en) 2004-06-22 2011-05-17 The Regents Of The University Of California Peptide-coated nanoparticles with graded shell compositions
US7229690B2 (en) 2004-07-26 2007-06-12 Massachusetts Institute Of Technology Microspheres including nanoparticles
US20110129944A1 (en) * 2005-01-17 2011-06-02 Agency For Science, Technology And Research Water-soluble nanocrystals and methods of preparing them
CN101208605A (zh) * 2005-05-04 2008-06-25 新加坡科技研究局 含有低分子量涂布剂的新型水溶性纳米晶及其制备方法
WO2007120762A2 (fr) * 2006-04-14 2007-10-25 Cambrios Technologies Corporation Particules fluorescentes liées à des ossatures multifonctionnelles et leurs utilisations
WO2008070459A2 (fr) * 2006-11-22 2008-06-12 Alnis Biosciences, Inc. Nanoparticules enrobées pour des systèmes aqueux
GB0813273D0 (en) * 2008-07-19 2008-08-27 Nanoco Technologies Ltd Method for producing aqueous compatible nanoparticles

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048616A (en) * 1993-04-21 2000-04-11 Philips Electronics N.A. Corp. Encapsulated quantum sized doped semiconductor particles and method of manufacturing same
WO1996014206A1 (fr) * 1994-11-08 1996-05-17 Spectra Science Corporation Substances d'affichage constituees de nanocristaux semi-conducteurs et dispositif d'affichage faisant appel a ces substances
US5908608A (en) * 1996-11-08 1999-06-01 Spectra Science Corporation Synthesis of metal chalcogenide quantum
JPH1187689A (ja) * 1997-09-04 1999-03-30 Fujitsu Ltd 量子ドットの製造方法
JP2980175B2 (ja) * 1997-11-20 1999-11-22 日本電気株式会社 量子ドット構造の製造方法及びそれを用いた半導体発光素子の製造方法
US5990479A (en) * 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes

Also Published As

Publication number Publication date
WO2000027365A1 (fr) 2000-05-18
WO2000027365A9 (fr) 2000-11-23

Similar Documents

Publication Publication Date Title
WO2000027365A8 (fr) Nanocristaux fonctionnalises et leur utilisation dans des systemes de detection
AU2001237057A1 (en) Functionalized encapsulated fluorescent nanocrystals
US6770220B1 (en) Production and use of luminescent microparticles and nanoparticles
AU8198091A (en) Method for amplifying a signal emitted by a luminescent compound
WO2001086299A3 (fr) Nanoparticules dopees servant de marqueurs biologiques
EP1418419A3 (fr) Cuve à circulation à fluorescence et a efficacité élevée
CA2433211A1 (fr) Determination de la condensation et de la pression du debut de cette condensation dans le condensat retrograde de champ petrolifere
EP2248912B8 (fr) Dosage biologique grande sensibilité permettant de détecter des pathogènes par fluorescence ameliorée par métal
ATE433498T1 (de) Detektion von neoplasien durch speichelanalyse
AU4701200A (en) A method of detecting an analyte using semiconductor nanocrystals
AU2001289853A1 (en) Multianalyte determination system and methods
EP0770969A3 (fr) Système et méthode d'amélioration du rapport signal sur bruit de balayeurs de codes à barres et de signes utilisant de l'encre fluorescente
SE428332B (sv) Forfarande for fluorescensspektroskopisk bestemning av biologiskt aktivt emne, sasom hapten, antikropp eller antigen
CA2223576A1 (fr) Piege optique de detection et de quantification de quantites subzeptomolaires d'analytes
WO2001044811A3 (fr) Particules utilisees a des fins diagnostiques et therapeutiques
WO2002057788A3 (fr) Detection de glucose dans des solutions contenant egalement un acide alpha-hydroxy ou une beta-diketone
AU7532501A (en) Method for increasing luminescence assay sensitivity
ES2163140T3 (es) Perfeccionamientos en sistemas de seguridad.
AU1717500A (en) Nanocrystals having polynucleotide strands and their use to form dendrimers in asignal amplification system
WO2003038408A3 (fr) Detection d'usure sur un substrat par utilisation d'un indicateur fluorescent
WO2006014576A3 (fr) Imagerie et detection biologique par fluorescence au moyen de nanocristaux semi-conducteurs stables dans l'eau
WO2003096014A3 (fr) Determination d'un analyte dans un support liquide
WO2004053467A3 (fr) Capteur optique pour determiner les concentrations en colorants et/ou en particules dans des milieux liquides ou gazeux et procede d'exploitation correspondant
SE9703780D0 (sv) Capillary based immunoassay
AU5382800A (en) Method for conducting chemiluminescent binding assay

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: C1

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: C1

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

CFP Corrected version of a pamphlet front page
CR1 Correction of entry in section i

Free format text: PAT. BUL. 20/2000 UNDER (30) REPLACE "NOT FURNISHED" BY "09/372729"

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: C2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

COP Corrected version of pamphlet

Free format text: PAGES 1/3-3/3, DRAWINGS, REPLACED BY NEW PAGES 1/3-3/3; DUE TO LATE TRANSMITTAL BY THE RECEIVING OFFICE

WWE Wipo information: entry into national phase

Ref document number: 1999965776

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1999965776

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWW Wipo information: withdrawn in national office

Ref document number: 1999965776

Country of ref document: EP